Roberta Kelly de Faria Souza, Isabel Chaves Silva Carvalho, Carolina Genúncio da Cunha Menezes Costa, Newton Soares da Silva, Cristina Pacheco-Soares
{"title":"光动力治疗后表面糖蛋白的改变。","authors":"Roberta Kelly de Faria Souza, Isabel Chaves Silva Carvalho, Carolina Genúncio da Cunha Menezes Costa, Newton Soares da Silva, Cristina Pacheco-Soares","doi":"10.1089/pho.2018.4464","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cell membranes have been identified as an important intracellular cancer treatment target, since the glycoconjugates present on the cell surface are involved in numerous cell functions. Photodynamic therapy (PDT) is a therapeutic modality employed in the treatment of tumors that uses visible light to activate a photosensitizer.</p><p><strong>Objective: </strong>This study analyzed the expression of surface carbohydrates after PDT with two different photosensitizers, 5-aminolevulinic acid (ALA) and Photosan-3.</p><p><strong>Methods: </strong>Mice were injected subcutaneously with 2 × 10<sup>5</sup> B16 cells. After 7-10 days, the presence of a tumor with a diameter of 3.6 mm was observed. Photosan-3<sup>®</sup> and 5-aminolevulinic acid-ALA were used in the PDT treatment. Control animals (not submitted to either laser treatment or photosensitizer injection) and treated animals were euthanized 15 days post-treatment. The tumors were irradiated with a red diode laser, λ = 655 nm, energy density of 10 J.cm<sup>-2</sup>, and power density of 45 mW.cm<sup>-2</sup>. After 2 weeks of treatment with PDT, the mice were euthanized, the tumors were collected, and the cell surfaces were labeled with lectins concanavalin A (ConA) and wheat germ agglutinin (WGA).</p><p><strong>Results: </strong>Fluorescence microscopy analysis of the cell surfaces with lectins ConA and WGA showed the presence of α-mannose and α-glucose.</p><p><strong>Conclusions: </strong>The combined effects of either Photosan-3 or ALA and red laser light on melanoma suggest an inhibitory glycosylation action from PDT on the surface of B16-F10 cells.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"452-456"},"PeriodicalIF":0.0000,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4464","citationCount":"1","resultStr":"{\"title\":\"Alteration of Surface Glycoproteins After Photodynamic Therapy.\",\"authors\":\"Roberta Kelly de Faria Souza, Isabel Chaves Silva Carvalho, Carolina Genúncio da Cunha Menezes Costa, Newton Soares da Silva, Cristina Pacheco-Soares\",\"doi\":\"10.1089/pho.2018.4464\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cell membranes have been identified as an important intracellular cancer treatment target, since the glycoconjugates present on the cell surface are involved in numerous cell functions. Photodynamic therapy (PDT) is a therapeutic modality employed in the treatment of tumors that uses visible light to activate a photosensitizer.</p><p><strong>Objective: </strong>This study analyzed the expression of surface carbohydrates after PDT with two different photosensitizers, 5-aminolevulinic acid (ALA) and Photosan-3.</p><p><strong>Methods: </strong>Mice were injected subcutaneously with 2 × 10<sup>5</sup> B16 cells. After 7-10 days, the presence of a tumor with a diameter of 3.6 mm was observed. Photosan-3<sup>®</sup> and 5-aminolevulinic acid-ALA were used in the PDT treatment. Control animals (not submitted to either laser treatment or photosensitizer injection) and treated animals were euthanized 15 days post-treatment. The tumors were irradiated with a red diode laser, λ = 655 nm, energy density of 10 J.cm<sup>-2</sup>, and power density of 45 mW.cm<sup>-2</sup>. After 2 weeks of treatment with PDT, the mice were euthanized, the tumors were collected, and the cell surfaces were labeled with lectins concanavalin A (ConA) and wheat germ agglutinin (WGA).</p><p><strong>Results: </strong>Fluorescence microscopy analysis of the cell surfaces with lectins ConA and WGA showed the presence of α-mannose and α-glucose.</p><p><strong>Conclusions: </strong>The combined effects of either Photosan-3 or ALA and red laser light on melanoma suggest an inhibitory glycosylation action from PDT on the surface of B16-F10 cells.</p>\",\"PeriodicalId\":20117,\"journal\":{\"name\":\"Photomedicine and laser surgery\",\"volume\":\"36 8\",\"pages\":\"452-456\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1089/pho.2018.4464\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photomedicine and laser surgery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/pho.2018.4464\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2018/7/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photomedicine and laser surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/pho.2018.4464","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/7/18 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
Alteration of Surface Glycoproteins After Photodynamic Therapy.
Background: Cell membranes have been identified as an important intracellular cancer treatment target, since the glycoconjugates present on the cell surface are involved in numerous cell functions. Photodynamic therapy (PDT) is a therapeutic modality employed in the treatment of tumors that uses visible light to activate a photosensitizer.
Objective: This study analyzed the expression of surface carbohydrates after PDT with two different photosensitizers, 5-aminolevulinic acid (ALA) and Photosan-3.
Methods: Mice were injected subcutaneously with 2 × 105 B16 cells. After 7-10 days, the presence of a tumor with a diameter of 3.6 mm was observed. Photosan-3® and 5-aminolevulinic acid-ALA were used in the PDT treatment. Control animals (not submitted to either laser treatment or photosensitizer injection) and treated animals were euthanized 15 days post-treatment. The tumors were irradiated with a red diode laser, λ = 655 nm, energy density of 10 J.cm-2, and power density of 45 mW.cm-2. After 2 weeks of treatment with PDT, the mice were euthanized, the tumors were collected, and the cell surfaces were labeled with lectins concanavalin A (ConA) and wheat germ agglutinin (WGA).
Results: Fluorescence microscopy analysis of the cell surfaces with lectins ConA and WGA showed the presence of α-mannose and α-glucose.
Conclusions: The combined effects of either Photosan-3 or ALA and red laser light on melanoma suggest an inhibitory glycosylation action from PDT on the surface of B16-F10 cells.
期刊介绍:
Photobiomodulation, Photomedicine, and Laser Surgery (formerly Photomedicine and Laser Surgery) is the essential journal for cutting-edge advances and research in phototherapy, low-level laser therapy (LLLT), and laser medicine and surgery. The Journal delivers basic and clinical findings and procedures to improve the knowledge and application of these techniques in medicine.