Parasitology International最新文献_第5页

Exploring tick-borne pathogens in community dogs in Nepal 探索尼泊尔社区犬的蜱传病原体。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-30 DOI: 10.1016/j.parint.2024.103003

Gita Sadaula Pandey , Chet Raj Pathak , Sunil Thapa , Amir Sadaula , Prajwol Manandhar , Abdelbaset Eweda Abdelbaset , Yongjin Qiu , Mackenzie L. Kwak , Naoki Hayashi , Nariaki Nonaka , Ryo Nakao

{"title":"Exploring tick-borne pathogens in community dogs in Nepal","authors":"Gita Sadaula Pandey , Chet Raj Pathak , Sunil Thapa , Amir Sadaula , Prajwol Manandhar , Abdelbaset Eweda Abdelbaset , Yongjin Qiu , Mackenzie L. Kwak , Naoki Hayashi , Nariaki Nonaka , Ryo Nakao","doi":"10.1016/j.parint.2024.103003","DOIUrl":"10.1016/j.parint.2024.103003","url":null,"abstract":"<div><div>Tick-borne pathogens (TBPs) in dogs are a major global health concern, with their zoonotic importance often being neglected in developing countries due to a lack of surveillance. This study aimed to highlight the incidence of six important TBPs belonging to the genera <em>Babesia, Theileria, Hepatozoon, Anaplasma, Ehrlichia,</em> and <em>Rickettsia</em> in a total of 230 community dogs from two sites: Lumbini and the Kathmandu Valley, of Nepal. A total of 75 (32.6 %) dogs were found to be infected with at least one TBP, with 11 (4.7 %) being co-infected with more than one TBP. The detection rates of TBPs were 13.9 % (<em>n</em> = 32) for <em>Ehrlichia canis,</em> 9.1 % (<em>n</em> = 21) for <em>Anaplasma platys,</em> 8.6 % (<em>n</em> = 20) for <em>Babesia vogeli</em>, and 6.5 % (<em>n</em> = 15) for <em>Babesia gibsoni</em>. None of the samples were positive for <em>Theileria, Hepatozoon,</em> or <em>Rickettsia</em>. There was a significant association between <em>A. platys</em> and <em>E. canis</em> infections, respectively, with the locations from which the samples were collected. Infections of TBPs in community dogs might be the source of infection for pet dogs or even humans in shared habitats. Further studies are needed to determine the prevalence and diversity of TBPs in dogs in other regions of Nepal. As some of these parasites are zoonotic, concerted efforts are required to raise awareness of, and control efforts for, these tick-borne pathogens.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"106 ","pages":"Article 103003"},"PeriodicalIF":1.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gnathostoma doloresi in domestic pigs in the Republic of Palau, 2020–2022 帕劳共和国家猪中的 Gnathostoma doloresi，2020-2022 年。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-26 DOI: 10.1016/j.parint.2024.103001

Ryo Suzuki , Toshihiro Tokiwa , Takuma Kasahara , Kashgar Rengulbai

引用次数: 0

Description of a new species of sucking louse Hoplopleura kuhnludwigi (Phthiraptera: Anoplura: Hoplopleuridae) from the Cape York rat Rattus leucopus cooktownensis (Rodentia: Muridae) in Australia using conventional methodology and novel epiflourescence microscopy 用常规方法和新型附着荧光显微镜对澳大利亚约克角大鼠（鼠目：鼠尾科）一新种吸虱的描述（翅翅目：鼠尾科）。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-26 DOI: 10.1016/j.parint.2024.103002

Constantin Constantinoiu , Wei Wang , Cadhla Firth , Richard Duffy , Jaqueline Picard , Bruce Gummow

{"title":"Description of a new species of sucking louse Hoplopleura kuhnludwigi (Phthiraptera: Anoplura: Hoplopleuridae) from the Cape York rat Rattus leucopus cooktownensis (Rodentia: Muridae) in Australia using conventional methodology and novel epiflourescence microscopy","authors":"Constantin Constantinoiu , Wei Wang , Cadhla Firth , Richard Duffy , Jaqueline Picard , Bruce Gummow","doi":"10.1016/j.parint.2024.103002","DOIUrl":"10.1016/j.parint.2024.103002","url":null,"abstract":"<div><div>A new species of sucking louse, <em>Hoplopleura kuhnludwigi</em> (Phthiraptera: Hoplopleuridae), from the Cape York rat <em>Rattus leucopus cooktownensis</em> Tate, 1951 (Rodentia: Muridae) is described and illustrated with photos and drawings. The host rat(s) were trapped in Northeast Queensland, Australia and identified using morphological characters and DNA sequencing. <em>Hoplopleura kuhnludwigi</em> is the first louse species to be described on <em>R. leucopus</em>. The morphology of the new louse species is contrasted with the morphology of <em>Hoplopleura</em> spp. previously described in Australian murids and shown to be different from other louse species that infest Australian native murids. Both conventional lice processing and staining methodology and a novel method, based on autofluorescence of chitin, have been used to describe and illustrate the diagnostic characters of <em>H. kuhnludwigi</em>. Processing of <em>H. kuhnludwigi</em> for epifluorescence microscopy (EM) is fast and easy, and the examination of the lice processed by this method can improve the accuracy of the description and identification of the lice species from genus <em>Hoplopleura</em> and potentially other louse species.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"106 ","pages":"Article 103002"},"PeriodicalIF":1.5,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology of Leishmaniasis: Risk factors for its pathology and infection 利什曼病的流行病学：病理和感染的风险因素

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-26 DOI: 10.1016/j.parint.2024.102999

Hirotomo Kato

{"title":"Epidemiology of Leishmaniasis: Risk factors for its pathology and infection","authors":"Hirotomo Kato","doi":"10.1016/j.parint.2024.102999","DOIUrl":"10.1016/j.parint.2024.102999","url":null,"abstract":"<div><div>Leishmaniasis is a neglected tropical disease caused by protozoan parasites of the genus <em>Leishmania</em>. About 20 species of <em>Leishmania</em> are pathogenic to humans, with the specific infecting species playing a crucial role in determining clinical outcomes. There are three main forms of disease: cutaneous, mucocutaneous and visceral leishmaniasis. In addition to the infecting species, it has recently been suggested that parasite strains and genetic factors affect disease manifestation and response to treatment. This suggests that infecting parasites are a crucial risk factor for the pathology of leishmaniasis. These parasites are transmitted by sand flies, of which more than 1000 species have been recorded. However, only approximately 10 % of these species are responsible for transmitting <em>Leishmania</em>, with each sand fly species typically transmitting specific species of <em>Leishmania</em>. Most <em>Leishmania</em> species are zoonotically transmitted by sand flies, with reservoir animals playing a crucial role in disease transmission and endemicity. This aspect of the disease ecology highlights the importance of considering both vectors and reservoir animals in endemic areas as risk factors for leishmaniasis. Our epidemiological studies on leishmaniasis focus mainly on South American countries. This review describes the epidemiological aspects of leishmaniasis in Ecuador and Peru, with a focus on pathological and infectious risks.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"105 ","pages":"Article 102999"},"PeriodicalIF":1.5,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitic helminths and protozoa: Treasure boxes of disease modifying anti-rheumatic drugs 寄生蠕虫和原生动物：改良抗风湿药物的宝库。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-26 DOI: 10.1016/j.parint.2024.103000

Yoshio Osada , Shoichi Shimizu, Kentaro Morita

引用次数: 0

A new focus of Taenia asiatica taeniasis in North Sumatra, Indonesia: Molecular confirmation and prevalence 印度尼西亚北苏门答腊的亚洲疟原虫新病灶：分子确认和流行率。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-23 DOI: 10.1016/j.parint.2024.102996

Dewi Masyithah Darlan , Hemma Yulfi , Sunna Vyatra Hutagalung , Merina Pangabean , Yunilda Andriyani , Irma Sepala Sari Siregar , Inke Nadia Diniyanti Lubis , Toni Wandra , Ivan Elisabeth Purba , Hiroshi Yamasaki , Yasuhito Sako

{"title":"A new focus of Taenia asiatica taeniasis in North Sumatra, Indonesia: Molecular confirmation and prevalence","authors":"Dewi Masyithah Darlan , Hemma Yulfi , Sunna Vyatra Hutagalung , Merina Pangabean , Yunilda Andriyani , Irma Sepala Sari Siregar , Inke Nadia Diniyanti Lubis , Toni Wandra , Ivan Elisabeth Purba , Hiroshi Yamasaki , Yasuhito Sako","doi":"10.1016/j.parint.2024.102996","DOIUrl":"10.1016/j.parint.2024.102996","url":null,"abstract":"<div><div>In the Samosir Island of North Sumatra, Indonesia, the prevalence of taeniasis caused by <em>Taenia asiatica</em> was reported as 2.2 %–20.7 % during 1972–2005. In 2014, another <em>T. asiatica</em>-endemic area was confirmed in the Silau Kahean sub-district of Simalungun District, which is geographically distant from Samosir Island. This study was conducted in the Raya Kahean sub-district of Simalungun District adjacent to the Silau Kahean sub-district in September 2023. The aim was to identify a new <em>T. asiatica</em>-endemic area based on molecular confirmation in addition to determining the prevalence of taeniasis. Men aged ≥18 years and palm plantation male farmers showed a higher prevalence of taeniasis, with an overall prevalence of 21.7 % (93/428). A total of 14 proglottids obtained from 14 tapeworm carriers were identified as <em>T. asiatica</em> by COX1-multiplex PCR analysis, and a new <em>T. asiatica</em>-endemic area was detected in the Raya Kahean sub-district. Genetic analyses of COX1 and <em>pold</em> revealed that <em>T. asiatica</em> from North Sumatra has a poor genetic diversity and is a descendant of the hybrids of <em>T. asiatica</em> and <em>T. saginata</em>. Infections were associated with a habitual consumption of raw pig and wild boar livers. Basic sanitary education, improvement of traditional food habits, and implementation of sentinel surveillance and treatment for tapeworm carriers in endemic areas are required to prevent and control this parasitic disease.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"105 ","pages":"Article 102996"},"PeriodicalIF":1.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dr. Jekyll and Mr. Hyde in sand fly saliva 沙蝇唾液中的杰基尔博士和海德先生。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-23 DOI: 10.1016/j.parint.2024.102998

Hirotomo Kato

{"title":"Dr. Jekyll and Mr. Hyde in sand fly saliva","authors":"Hirotomo Kato","doi":"10.1016/j.parint.2024.102998","DOIUrl":"10.1016/j.parint.2024.102998","url":null,"abstract":"<div><div>Phlebotomine sand flies are very small hematophagous insects, and some species transmit human pathogens, such as <em>Leishmania</em> protozoa. Similar to other hematophagous insects, sand flies possess unique bioactive substances in their saliva to facilitate blood feeding. Active transcriptome and proteome analyses revealed that sand flies have unique molecules in their saliva that are structurally different from those of other arthropods. These components exert anticoagulant, antiplatelet, vasodilator, and anti-inflammatory effects on the host, and the unique bioactivities of each molecule are currently being characterized. Several bioactivities of salivary components have been associated with the exacerbation of <em>Leishmania</em> infection, and investigations on the molecular mechanisms responsible are underway. On the other hand, host immunity to some salivary components has been shown to confer protection against <em>Leishmania</em> infection, suggesting the potential of salivary components as vaccine candidates. Although some negative effects of protection by sand fly saliva have been reported, the identification of suitable immunogens and elucidation of appropriate protective immunity are expected for the development of a sand fly saliva vaccine against <em>Leishmania</em> infection.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"105 ","pages":"Article 102998"},"PeriodicalIF":1.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in identifying and characterizing secretory proteins of Toxoplasma gondii by CRISPR-based screening 通过基于 CRISPR 的筛选鉴定和描述弓形虫分泌蛋白的最新进展。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-23 DOI: 10.1016/j.parint.2024.102997

Yuta Tachibana , Masahiro Yamamoto

{"title":"Recent advances in identifying and characterizing secretory proteins of Toxoplasma gondii by CRISPR-based screening","authors":"Yuta Tachibana , Masahiro Yamamoto","doi":"10.1016/j.parint.2024.102997","DOIUrl":"10.1016/j.parint.2024.102997","url":null,"abstract":"<div><div>The apicomplexan parasite, <em>Toxoplasma gondii</em>, develops unique secretory organelles, such as micronemes, rhoptries, and dense granules, which do not exist in other well-studied eukaryotic organisms. These secretory organelles are key features of apicomplexan parasites and discharge various proteins that are essential for invasion, replication, egress, host-parasite interactions, and virulence. Many studies have therefore focused on identifying and characterizing the proteins secreted by <em>T. gondii</em> that play essential roles in pathology and that can be targeted for therapeutics and vaccine development. The recent development of functional genetic screens based on CRISPR/Cas9 technology has revolutionized this field and has enabled the identification of genes that contribute to parasite fitness <em>in vitro</em> and <em>in vivo</em>. Consequently, characterization of genes identified by unbiased CRISPR screens has revealed novel aspects of apicomplexan biology. In this review, we describe the development of CRIPSR-based screening technology for <em>T. gondii</em>, and recent advances in our understanding of secretory proteins identified and characterized by CRISPR-based screening.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"105 ","pages":"Article 102997"},"PeriodicalIF":1.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxoplasma GRA15 expression on dendritic cells inhibits B cell differentiation and antibody production 弓形虫 GRA15 在树突状细胞上的表达会抑制 B 细胞分化和抗体生成。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-17 DOI: 10.1016/j.parint.2024.102995

Yuki Nakayama , Fumiaki Ihara , Daisuke Okuzaki , Yoshifumi Nishikawa , Miwa Sasai , Masahiro Yamamoto

{"title":"Toxoplasma GRA15 expression on dendritic cells inhibits B cell differentiation and antibody production","authors":"Yuki Nakayama , Fumiaki Ihara , Daisuke Okuzaki , Yoshifumi Nishikawa , Miwa Sasai , Masahiro Yamamoto","doi":"10.1016/j.parint.2024.102995","DOIUrl":"10.1016/j.parint.2024.102995","url":null,"abstract":"<div><div>One of the dense granule proteins named GRA15 in <em>Toxoplasma gondii</em> (<em>T. gondii</em>), is known to support an innate immune response in host through activation of NF-κB. However, little is known about advantages of GRA15 for parasites. By examining the role of GRA15 in the host-parasite interactions, it was clarified that GRA15 in <em>T. gondii</em> suppressed acquired immune responses in host. Wild-type parasite infection to C57BL/6 mice resulted in lower titers of <em>T. gondii</em> antibody and lower plasma cell counts compared to Δgra15 <em>T. gondii</em>. To identify host cells in which GRA15 acts to suppress antibody production, we generated conditional knock-in mice that express GRA15 in specific cell lineages. Anti-<em>T. gondii</em> antibodies were not reduced in macrophages of conditional knock-in mice after infection with Δgra15 <em>T. gondii</em>, while the production of <em>T. gondii</em> antibody was suppressed in dendritic cells of the conditional knock-in mice (CD11c-Cre/GRA15cKI). In the CD11c-Cre/GRA15cKI immunized with ovalbumin (OVA), the titers of anti-OVA antibody were reduced compared to control mice. Furthermore, the number of OVA antigen-specific T cells was also decreased in CD11c-Cre/GRA15cKI. These data showed that GRA15 in dendritic cells suppressed T cell-mediated humoral immunity. These findings might implicate the pathological significance of GRA15 and facilitate <em>Toxoplasma</em> vaccines production.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"105 ","pages":"Article 102995"},"PeriodicalIF":1.5,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-10 is not required for the alleviation of collagen-induced arthritis by non-lethal malarial infection in mice 小鼠非致命性疟疾感染对胶原蛋白诱导的关节炎的缓解不需要IL-10。

IF 1.5 4区医学

Parasitology International Pub Date : 2024-11-17 DOI: 10.1016/j.parint.2024.102993

Shoichi Shimizu , Junko Shin , Takuma Ota , Hirofumi Kondo , Susumu Nakae , Katsuko Sudo , Eman M. Gaballah , Kentaro Morita , Yoshio Osada

{"title":"IL-10 is not required for the alleviation of collagen-induced arthritis by non-lethal malarial infection in mice","authors":"Shoichi Shimizu , Junko Shin , Takuma Ota , Hirofumi Kondo , Susumu Nakae , Katsuko Sudo , Eman M. Gaballah , Kentaro Morita , Yoshio Osada","doi":"10.1016/j.parint.2024.102993","DOIUrl":"10.1016/j.parint.2024.102993","url":null,"abstract":"<div><div>We previously reported that <em>Plasmodium yoelii</em> 17XNL (Py), a non-lethal rodent malarial parasite, could suppress collagen-induced arthritis (CIA) and increase the production of T cell-derived interleukin (IL)-10. However, it remained unclear whether IL-10 is essential for the Py-induced suppression of CIA. Male IL-10 knockout (KO) DBA/1 J mice were immunized with bovine type II collagen (CII) and subsequently infected with Py at one week post-immunization. The development of arthritis was evaluated by an arthritis score up to 6 weeks post-immunization. At 3 weeks post-immunization, cytokine production from splenocytes and serum anti-CII IgG/IgG1/IgG2a levels were compared between non-infected control mice and Py-infected mice. Py infection inhibited the development of CIA in IL-10KO mice until 4 weeks post-immunization, after which the arthritis score reached levels comparable with the control mice. Both pro-arthritic (IL-17 and TNF-α) and anti-arthritic (IFN-γ and IL-4) cytokines were down-regulated during the periods of parasitemia, while no significant differences were observed in levels of anti-CII IgG antibodies. Our findings indicate that Py alleviates CIA via IL-10-independent mechanisms.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102993"},"PeriodicalIF":1.5,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0