Parasitology最新文献_第8页

Occurrence of Trichinella spiralis in farmed wild boars (Sus scrofa): an underrated risk in China. 养殖野猪（Sus scrofa）中旋毛虫螺旋体的发生：在中国被低估的风险。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-12-03 DOI: 10.1017/S0031182024001288

Nian-Zhang Zhang, Meng Wang, Wei-Gang Chen, Wen-Yan Gai, Thanh Thi Ha Dao, Ting-Ting Li, Wen-Hui Li, Hai-Rui Zhang, Hong Yin, Nguyen Thi Bich Thuy, Bao-Quan Fu

{"title":"Occurrence of Trichinella spiralis in farmed wild boars (Sus scrofa): an underrated risk in China.","authors":"Nian-Zhang Zhang, Meng Wang, Wei-Gang Chen, Wen-Yan Gai, Thanh Thi Ha Dao, Ting-Ting Li, Wen-Hui Li, Hai-Rui Zhang, Hong Yin, Nguyen Thi Bich Thuy, Bao-Quan Fu","doi":"10.1017/S0031182024001288","DOIUrl":"10.1017/S0031182024001288","url":null,"abstract":"Natural infection by Trichinella sp. has been reported in humans and more than 150 species of animals, especially carnivorous and omnivorous mammals. Although the presence of Trichinella sp. infection in wild boars (Sus scrofa) has been documented worldwide, limited information is known about Trichinella circulation in farmed wild boars in China. This study intends to investigate the prevalence of Trichinella sp. in farmed wild boars in China. Seven hundred and sixty-one (761) muscle samples from farmed wild boars were collected in Jilin Province of China from 2017 to 2020. The diaphragm muscles were examined by artificial digestion method. The overall prevalence of Trichinella in farmed wild boars was 0.53% [95% confidence interval (CI): 0.51–0.55]. The average parasite loading was 0.076 ± 0.025 larvae per gram (lpg), and the highest burden was 0.21 lpg in a wild boar from Fusong city. Trichinella spiralis was the only species identified by multiplex polymerase chain reaction. The 5S rDNA inter-genic spacer region of Trichinella was amplified and sequenced. The results showed that the obtained sequence (GenBank accession number: OQ725583) shared 100% identity with the T. spiralis HLJ isolate (GenBank accession number: MH289505). Since the consumption of farmed wild boars is expected to increase in the future, these findings highlight the significance of developing exclusive guidelines for the processing of slaughtered farmed wild boar meat in China.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1351-1354"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and analysis of the drug resistance-associated protein phosphoglycerate kinase of Eimeria tenella. 天牛埃默氏菌耐药性相关蛋白磷酸甘油酸激酶的分子特征和分析。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-10-18 DOI: 10.1017/S0031182024001355

Yu Yu, Wenhao Huang, Qiping Zhao, Shunhai Zhu, Hui Dong, Hongyu Han

{"title":"Molecular characterization and analysis of the drug resistance-associated protein phosphoglycerate kinase of Eimeria tenella.","authors":"Yu Yu, Wenhao Huang, Qiping Zhao, Shunhai Zhu, Hui Dong, Hongyu Han","doi":"10.1017/S0031182024001355","DOIUrl":"10.1017/S0031182024001355","url":null,"abstract":"Coccidiosis is a parasitic disease caused by Eimeria spp., and the emergence of drug resistance has seriously affected the control of the disease. Using RNA-seq, we previously found that phosphoglycerate kinase of Eimeria tenella (EtPGK) was differentially downregulated in diclazuril-resistant (DZR) and maduramicin-resistant (MRR) strains compared with drug-sensitive (DS) strain. In this study, we further analysed the characteristics and functions of EtPGK to find the possible mechanism of drug resistance of E. tenella. Quantitative real-time PCR (qRT-PCR) and western blot found that EtPGK was highly expressed in sporulated oocysts, followed by sporozoites and second-generation merozoites of E. tenella. Indirect immunofluorescence localization showed that EtPGK was located mainly in the cytoplasm and on the surface of the parasites. Invasion inhibition assays showed that anti-rEtPGK antibody significantly inhibited the invasion of parasites. Further studies using qRT-PCR and western blot found that the transcription and translation levels of EtPGK were downregulated in both resistant (DZR and MRR) strains compared with the DS strain, and the transcription level correlated negatively with the drug concentration. The enzyme activity assay revealed that EtPGK enzyme activity was decreased in the DZR strain compared with the DS strain. qRT-PCR revealed that the mRNA transcription level of EtPGK was significantly downregulated in the field DZR strain and salinomycin-resistant strain compared with the DS strain. These results suggested that EtPGK has other important roles that are separate and distinct from its function in glycolysis, and it might be involved in the development of drug resistance of E. tenella.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1371-1379"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of optimum scopes of environmental drivers for schistosome-transmitting Oncomelania hupensis using agent-based model in Dongting Lake Region, China. 利用基于代理的模型确定中国洞庭湖区环境驱动因素对血吸虫传播的最佳影响范围。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-11-11 DOI: 10.1017/S0031182024001306

Junhui Huang, Jiamin Wang, Yanfeng Gong, Ning Xu, Yu Zhou, Liyun Zhu, Liang Shi, Yue Chen, Qingwu Jiang, Yibiao Zhou

{"title":"Identification of optimum scopes of environmental drivers for schistosome-transmitting Oncomelania hupensis using agent-based model in Dongting Lake Region, China.","authors":"Junhui Huang, Jiamin Wang, Yanfeng Gong, Ning Xu, Yu Zhou, Liyun Zhu, Liang Shi, Yue Chen, Qingwu Jiang, Yibiao Zhou","doi":"10.1017/S0031182024001306","DOIUrl":"10.1017/S0031182024001306","url":null,"abstract":"Oncomelania hupensis (O. hupensis), the sole intermediate host of Schistosoma japonicum, greatly influence the prevalence and distribution of schistosomiasis japonica. The distribution area of O. hupensis has remained extensive for numerous years. This study aimed to establish a valid agent-based model of snail density and further explore the environmental conditions suitable for snail breeding. A marshland with O. hupensis was selected as a study site in Dongting Lake Region, and snail surveys were monthly conducted from 2007 to 2016. Combined with the data from historical literature, an agent-based model of snail density was constructed in NetLogo 6.2.0 and validated with the collected survey data. BehaviorSpace was used to identify the optimal ranges of soil temperature, pH, soil water content, and vegetation coverage for snail growth, development and reproduction. An agent-based model of snail density was constructed and showed a strong agreement with the monthly average snail density from the field surveys. As soil temperature increased, the snail density initially rose before declining, reaching its peak at around 21°C. There were similar variation patterns for other environmental factors. The findings from the model suggested that the optimum ranges of soil temperature, pH, soil water content and vegetation coverage were 19°C to 23 °C, 6.4 to 7.6, 42% to 75%, and 70% to 93%, respectively. A valid agent-based model of snail density was constructed, providing more objective information about the optimum ranges of environmental factors for snail growth, development and reproduction.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1355-1363"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Migratory birds have a distinct haemosporidian community and are temporally decoupled from vector abundance at a stopover site. 候鸟有独特的血孢子虫群落，在时间上与中途停留地的病媒丰度脱钩。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-11-11 DOI: 10.1017/S0031182024001239

Spencer C Galen, Emily Ostrow, Suravi Ray, Marissa Henry, Janice Dispoto, Alison Fetterman, Lisa Kiziuk, Jason D Weckstein

{"title":"Migratory birds have a distinct haemosporidian community and are temporally decoupled from vector abundance at a stopover site.","authors":"Spencer C Galen, Emily Ostrow, Suravi Ray, Marissa Henry, Janice Dispoto, Alison Fetterman, Lisa Kiziuk, Jason D Weckstein","doi":"10.1017/S0031182024001239","DOIUrl":"10.1017/S0031182024001239","url":null,"abstract":"Migratory animals likely play an important role in the geographic spread of parasites. In fact, a common assumption is that parasites are potentially transmitted by migratory animals at temporary stopover sites along migratory routes, yet very few studies have assessed whether transmission at stopover sites can or does occur. We investigated the potential for a group of vector-transmitted parasites, the avian haemosporidians, to be transmitted during migratory stopover periods at Rushton Woods Preserve in Pennsylvania, USA. Using an analysis of 1454 sampled avian hosts, we found that while a core group of abundant haemosporidians was shared between local breeding birds and passing migrants, the parasite community of migratory birds at Rushton was distinct from that of local breeding birds and showed similarity to a previously sampled boreal forest haemosporidian community. Haemosporidians that were unique to passing migratory birds were associated with sampling sites in North America with cooler summer temperatures than haemosporidians that are transmitted at Rushton, suggesting that the transmission of these parasites may be restricted to high-latitude regions outside of our temperate stopover site. We also found that the abundance of mosquitoes in our study region is offset from that of migratory bird abundance during avian migratory periods, with the peak period of bird migration occurring during periods of low mosquito activity. Collectively, these findings suggest that although abundant haemosporidians are possibly transmitted between local and passing migratory birds, a combination of biotic and abiotic factors may constrain haemosporidian transmission during avian stopover at our study site.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1326-1335"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomy and systematics of Emprostiotrema Cianferoni and Ceccolini, 2021 (Digenea: Emprostiotrematidae), parasites of rabbitfish (Siganidae) from the Indo-West Pacific marine region. 印度-西太平洋海洋区域兔鱼（Siganidae）寄生虫 Emprostiotrema Cianferoni and Ceccolini, 2021（Digenea: Emprostiotrematidae）的分类和系统学。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-11-20 DOI: 10.1017/S0031182024001252

Daniel C Huston, Scott C Cutmore, Thomas H Cribb, Pierre Sasal, Russell Q-Y Yong

{"title":"Taxonomy and systematics of Emprostiotrema Cianferoni and Ceccolini, 2021 (Digenea: Emprostiotrematidae), parasites of rabbitfish (Siganidae) from the Indo-West Pacific marine region.","authors":"Daniel C Huston, Scott C Cutmore, Thomas H Cribb, Pierre Sasal, Russell Q-Y Yong","doi":"10.1017/S0031182024001252","DOIUrl":"10.1017/S0031182024001252","url":null,"abstract":"Emprostiotrema contains just 3 species: E. fusum, E. kuntzi and E. sigani. As adults, all 3 species infect rabbitfishes (Siganidae: Siganus). New collections from 11 species of Siganus from northern Australia, Indonesia, New Caledonia, French Polynesia, Palau and Japan enabled an exploration of species composition within this genus. Phylogenetic analyses demonstrate a deep distinction between 2 major clades; clade 1 comprises most of the sequences of specimens from Australia as well as all of those from Japan, Palau and New Caledonia and clade 2 comprises all sequences of specimens from French Polynesia, 2 sequences from Australia and the single sequence from Bali. In all analyses, both major clades have genetic structuring leading to distinct geographic lineages. Morphologically, specimens relating to clades 1 and 2 differ but overlap in body shape, oral sucker and egg size. Principle component analysis shows a general (but not complete) separation between specimens relating to the 2 clades. We interpret the 2 clades as representing 2 species: clade 1 is identified as E. fusum and is reported in this study from 10 species of siganids from Australia, Japan, Palau and New Caledonia; clade 2 is described as E. gotozakiorum n. sp., for all specimens from French Polynesia and rare specimens from Australia and Indonesia. We recognize E. sigani as a junior synonym of E. fusum. Although species of Emprostiotrema occur widely in the tropical Indo-Pacific, they have not been detected from Ningaloo Reef (Western Australia), the southern Great Barrier Reef or Moreton Bay (southern Queensland).","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1336-1350"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first mitogenome of the genus Amphalius (Siphonaptera: Ceratophyllidae) and its phylogenetic implications - CORRIGENDUM.

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 DOI: 10.1017/S0031182024001604

Ju Pu, Xiaoxia Lin, Wenge Dong

引用次数: 0

Analysis of the mitochondrial genome to determine the origins and pathways of entry of Angiostrongylus cantonensis in continental Europe (Valencia, Spain). 欧洲大陆（西班牙瓦伦西亚）广东管圆线虫起源和进入途径的线粒体基因组分析。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-12-02 DOI: 10.1017/S0031182024001318

Mercedes Gómez-Samblás, Beatriz Navarro-Dominguez, Sandra Sáez-Durán, Antonio Osuna, Rubén Bueno-Marí, María Teresa Galán-Puchades, Màrius V Fuentes

{"title":"Analysis of the mitochondrial genome to determine the origins and pathways of entry of Angiostrongylus cantonensis in continental Europe (Valencia, Spain).","authors":"Mercedes Gómez-Samblás, Beatriz Navarro-Dominguez, Sandra Sáez-Durán, Antonio Osuna, Rubén Bueno-Marí, María Teresa Galán-Puchades, Màrius V Fuentes","doi":"10.1017/S0031182024001318","DOIUrl":"10.1017/S0031182024001318","url":null,"abstract":"Angiostrongylus cantonensis, the rat lungworm, is a zoonotic parasite mainly of rats which act as definitive hosts. If humans become accidentally infected, the nematode is capable of migrating to the brain causing meningoencephalitis. Intermediate hosts are snails and slugs. Although originating from mainland China, A. cantonensis has now spread to various countries and continents. The precise timing of its departure from mainland China remains uncertain although it is often associated with significant historical events or migratory movements. The exit of A. cantonensis from mainland China is believed to have occurred in a singular event, followed by its divergence into 2 distinct clades: clade I, originating from mainland China, and clade II, representing global spread. Angiostrongylus cantonensis was first identified in continental Europe in 2021, specifically in Valencia, Spain. Illumina genome sequencing of 7 individuals isolated from rats captured in 2 different districts in the city of Valencia was carried out. The complete mitochondrial genome was assembled and compared with published A. cantonensis mitochondrial genomes through Bayesian phylogenetic analysis, both for complete mitochondrial genomes and for the cytochrome c oxidase I gene, given its widespread use for identification of the species. The findings revealed the presence of 2 different A. cantonensis haplotypes in the rats studied in Valencia, both belonging to clade II. In 2 rats both clades were present.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1364-1370"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isopods infesting Atlantic bonefish (Albula vulpes) host novel viruses, including reoviruses related to global pathogens, and opportunistically feed on humans. 大西洋骨鱼（Albula vulpes）体内的等足类寄生着新型病毒，包括与全球病原体有关的再病毒，并伺机以人类为食。

IF 2.1 3区医学

Parasitology Pub Date : 2024-10-01 Epub Date: 2024-11-20 DOI: 10.1017/S003118202400146X

Tony L Goldberg, Addiel U Perez, Lewis J Campbell

{"title":"Isopods infesting Atlantic bonefish (Albula vulpes) host novel viruses, including reoviruses related to global pathogens, and opportunistically feed on humans.","authors":"Tony L Goldberg, Addiel U Perez, Lewis J Campbell","doi":"10.1017/S003118202400146X","DOIUrl":"10.1017/S003118202400146X","url":null,"abstract":"Isopods infest fish worldwide, but their role as disease vectors remains poorly understood. Here, we describe infestation of Atlantic bonefish (Albula vulpes) in Belize with isopods in two of three locations studied, with infestation rates of 15 and 44%. Isopods fed aggressively, and infested fish showed missing scales and scars. Gross morphologic and molecular phylogenetic analyses revealed the isopods to cluster within the family Aegidae and to be most closely related to members of the genus Rocinela, which are globally distributed micro-predators of fish. Metagenomic analysis of 10 isopods identified 11 viruses, including two novel reoviruses (Reovirales) in the families Sedoreoviridae and Spinareoviridae. The novel sedoreovirus clustered phylogenetically within an invertebrate-specific clade of viruses related to the genus Orbivirus, which contains arboviruses of global concern for mammal health. The novel spinareovirus clustered within the fish-infecting genus Aquareovirus, which contains viruses of global concern for fish health. Metagenomic analyses revealed no evidence of infection of bonefish with the novel aquareovirus, suggesting that viremia in bonefish is absent, low, or transient, or that isopods may have acquired the virus from other fish. During field collections, isopods aggressively bit humans, and blood meal analysis confirmed that isopods had fed on bonefish, other fish, and humans. Vector-borne transmission may be an underappreciated mechanism for aquareovirus transmission and for virus host switching between fish and other species, which has been inferred across viral families from studies of deep virus evolution.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1386-1396"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evaluation of African animal trypanosomiasis control strategies in remote communities of Eastern Zambia. 评估赞比亚东部偏远社区的非洲动物锥虫病控制策略。

IF 2.1 3区医学

Parasitology Pub Date : 2024-09-01 Epub Date: 2024-10-30 DOI: 10.1017/S0031182024001070

Gloria M Mulenga, Kalinga Chilongo, Chrisborn Mubamba, Bruce Gummow

{"title":"An evaluation of African animal trypanosomiasis control strategies in remote communities of Eastern Zambia.","authors":"Gloria M Mulenga, Kalinga Chilongo, Chrisborn Mubamba, Bruce Gummow","doi":"10.1017/S0031182024001070","DOIUrl":"10.1017/S0031182024001070","url":null,"abstract":"Communities living in African animal trypanosomiasis (AAT) endemic areas of Zambia use several control strategies to protect their livestock from the devastating effects of trypanosomiasis. Several studies have reported the effectiveness of trypanosomiasis control strategies based on retrospective data. In this study, we assessed incidence rates of AAT in cattle (n = 227) using a prospective cohort study comprising 4 treatment groups, i.e., Diminazene aceturate, Isometamidium chloride, Cyfluthrin pour-on and Cypermethrin treated targets. The study was conducted in Mambwe district in Eastern Zambia between February 2019 and March 2020. The endemic prevalence of AAT for each group was determined using ITS-PCR prior to application of treatments. High endemic trypanosome pre-treatment rates were found in all Groups (Diminazene aceturate (61%), Isometamidium chloride (48%), Cyfluthrin pour-on (87%) and Cypermethrin targets (72%)). The overall apparent prevalence for the Mambwe district was 67% (152/227) and true prevalence at 95%CI was 63–71%. Once treatments were implemented, 12 monthly follow-ups were conducted. The average monthly incidence rates without standardization recorded: Diminazene aceturate (67%) Isometamidium chloride (35%), Cyfluthrin pour-on (55%) and Cypermethrin targets (61%). Incidence rates were standardized considering the endemic level of disease for each Group and the average standardized monthly incidence rate in the Diminazene aceturate Group was 7%; the Isometamidium chloride Group −13%; the Cyfluthrin Group −26%; and the Cypermethrin target Group, −17%. All Groups showed a decrease in incidence of AAT over the period of the study with the Cyfluthrin group showing to be the most effective in reducing AAT incidence in cattle.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1269-1276"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of CD1a immunostaining in the diagnosis of cutaneous leishmaniasis caused by Leishmania donovani in Sri Lanka. 评估 CD1a 免疫染色法在诊断斯里兰卡由多诺万利什曼病引起的皮肤利什曼病中的应用。

IF 2.1 3区医学

Parasitology Pub Date : 2024-09-01 Epub Date: 2024-11-11 DOI: 10.1017/S0031182024000799

Hasna Riyal, Nilakshi Samaranayake, Priyani Amarathunga, Deepani Munidasa, Nadira Karunaweera

{"title":"Evaluation of CD1a immunostaining in the diagnosis of cutaneous leishmaniasis caused by Leishmania donovani in Sri Lanka.","authors":"Hasna Riyal, Nilakshi Samaranayake, Priyani Amarathunga, Deepani Munidasa, Nadira Karunaweera","doi":"10.1017/S0031182024000799","DOIUrl":"10.1017/S0031182024000799","url":null,"abstract":"Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease, routinely diagnosed by direct light microscopy. The sensitivity of this method is dependent on the number of parasites present in the lesion. Immunoexpression of CD1a surface antigen by Leishmania amastigotes and its application as a diagnostic tool has been recently demonstrated in several species including Leishmania major, Leishmania tropica and Leishmania infantum. Leishmania donovani is the only reported species in Sri Lanka primarily causing CL and its CD1a status remains unexplored. We studied CD1a expression by amastigotes of L. donovani in skin biopsies from 116 patients with suspected CL. The biopsy sections were stained with CD1a clones O10 and MTB1 separately. Slit skin smear (SSS) results were considered the gold standard for diagnosis of CL. 103 cases were confirmed through SSS where 73 of them showed positive parasite staining for CD1a clone MTB1 with 70.9% sensitivity. Positivity was seen mostly in parasites closer to the epidermis. CD1a clone O10 failed to detect any amastigotes. Test sensitivity improved to 74.1% when the analysis was applied only to patients with low/no discernible Leishman-Donovan (LD) bodies in histology. Our findings show that CD1a clone MTB1 successfully stains amastigotes of L. donovani species and can be used as a supplementary diagnostic tool in detecting CL, especially when LD bodies are low in number. This method could be validated to detect other forms of leishmaniasis caused by L. donovani in Indian and sub-Saharan regions.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1148-1153"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0