Parasitology最新文献_第7页

Fecal metabarcoding vs traditional invasive survey to access the diversity of anuran endoparasites and diet. 粪便元条形码与传统侵入性调查对比，以获取动物体内寄生虫和饮食的多样性。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-18 DOI: 10.1017/S0031182025100292

Amanda Dudczak, Rafael Euclydes, Emanuel Razzolini, Hernani Fernandes Magalhães de Oliveira, Melissa Ingala, Karla Magalhães Campião

{"title":"Fecal metabarcoding vs traditional invasive survey to access the diversity of anuran endoparasites and diet.","authors":"Amanda Dudczak, Rafael Euclydes, Emanuel Razzolini, Hernani Fernandes Magalhães de Oliveira, Melissa Ingala, Karla Magalhães Campião","doi":"10.1017/S0031182025100292","DOIUrl":"10.1017/S0031182025100292","url":null,"abstract":"Identifying interactions between species is essential for understanding ecosystem dynamics. With their central position in trophic networks, anurans underscore the importance of studying their interactions with other organisms. Traditionally, collecting and describing anuran helminth parasites rely on lethal methods, posing challenges for studying threatened species. In this study, we tested the effectiveness of non-invasive fecal metabarcoding and compared its accuracy to traditional invasive methods for identifying parasites and dietary components. We collected anurans from 6 families in the Brazilian Atlantic Rainforest and analysed their feces using the 18S marker while performing necropsies for traditional identification. Traditional methods identified 12 parasite taxa and 3 dietary items at lower taxonomic resolution. Fecal metabarcoding, on the other hand, revealed greater diversity and fine taxonomic resolution for dietary items, although with lower accuracy for parasites due to database limitations. The metabarcoding approach demonstrated a high potential for non-lethal biodiversity assessments, offering a more comprehensive view of dietary diversity and a viable alternative for studying parasites in vulnerable populations. However, its effectiveness depends on improving reference databases, especially for parasite taxa. The advancement of non-invasive approaches that integrate parasitological data holds great potential to improve conservation strategies and enhance the ecological understanding of amphibian-parasite interactions.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-8"},"PeriodicalIF":2.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross sectional assessment of feasibility and acceptability in screening for female genital schistosomiasis (FGS) in two clinical settings in east province, Cameroon. 在喀麦隆东部省的两个临床环境中筛查女性生殖器血吸虫病（FGS）的可行性和可接受性横断面评估。

IF 2.1 3区医学

Parasitology Pub Date : 2025-06-16 DOI: 10.1017/S0031182025100255

Sandrine Nyotue, Laura Ciaffi, Aimé Assigui, Louis-Albert Tchuem Tchuente, Bodo Randraniasolo, John Russel Stothard, Jutta Reinhard-Rupp, Vanessa Christinet

引用次数: 0

The dsRNA-dependent kinase (PKR) inhibits the growth of Leishmania major via NF-κB-mediated genes. dsRNA依赖性激酶（PKR）通过nf - kb介导的基因抑制利什曼原虫的生长。

IF 2.1 3区医学

Parasitology Pub Date : 2025-06-16 DOI: 10.1017/S0031182025100218

Áislan de Carvalho Vivarini, Bianca Cristina Duarte Vivarini, Yuri Nunes Oliveira, Flavia Thiebaut, Evelize Folly das Chagas

{"title":"The dsRNA-dependent kinase (PKR) inhibits the growth of Leishmania major via NF-κB-mediated genes.","authors":"Áislan de Carvalho Vivarini, Bianca Cristina Duarte Vivarini, Yuri Nunes Oliveira, Flavia Thiebaut, Evelize Folly das Chagas","doi":"10.1017/S0031182025100218","DOIUrl":"10.1017/S0031182025100218","url":null,"abstract":"Parasites of the Leishmania species have been observed to infect macrophages and thereby modulate the host microbicidal responses, resulting in a wide spectrum of diseases. A comprehensive experimental mapping of the relationship between the double-stranded RNA protein kinase R (PKR) and NF-κB pathways in the outcome of the infection was conducted in an effort to improve the understanding of the biology associated with the parasites-host cell interaction. The results showed that in the absence of PKR and Type I Interferon (IFN) signalling, L. major infection was enhanced. The levels of PKR and gene promoter activation were evaluated. The results showed that infection did not induce PKR expression by inhibiting the phosphorylation of STAT1 and subsequent binding in the PKR promoter. However, infection induced PKR phosphorylation but did not prevent subsequent signalling through this pathway. To address the role of activation of these signalling, the induction of PKR-dependent gene expression was examined. Activation of the classical p65/p50 dimer was found to be dependent on the PKR in the L. major infection, which was essential for the induction of iNOS, IFNβ and tumour necrosis factor expression. In addition, macrophages treated with nuclear factor-kB inhibitors were more susceptible to infection. Furthermore, translocation of the p65/p50 to the promoters of these genes increased in a PKR-dependent manner. Collectively, these results suggest that macrophages retain their ability to induce important downstream effectors in PKR signalling. These effectors contribute to protection in pathogenesis, reducing parasite proliferation and regulating the inflammatory genes that, consequently, modulate the activation state of macrophages during infection.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-14"},"PeriodicalIF":2.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular survey of taeniid cestodes with special emphasis on Echinococcus species in free-roaming dogs and wild carnivores in Armenia. 亚美尼亚流浪狗和野生食肉动物绦虫的分子调查，特别强调棘球绦虫的种类。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S0031182025100474

Hasmik Gevorgyan, Sargis A Aghayan, Alexander Malkhasyan, Manan Asikyan, Thomas Romig, Marion Wassermann

{"title":"Molecular survey of taeniid cestodes with special emphasis on Echinococcus species in free-roaming dogs and wild carnivores in Armenia.","authors":"Hasmik Gevorgyan, Sargis A Aghayan, Alexander Malkhasyan, Manan Asikyan, Thomas Romig, Marion Wassermann","doi":"10.1017/S0031182025100474","DOIUrl":"10.1017/S0031182025100474","url":null,"abstract":"Echinococcosis, caused by cestodes of the genus Echinococcus, poses significant public health and veterinary concerns globally. In Armenia, cystic echinococcosis (CE) is well-documented in livestock and humans, while alveolar echinococcosis (AE) has long been considered non-endemic. However, a recent retrospective study identified human AE cases, suggesting an underestimation of the parasite's presence. To address knowledge gaps, a pilot survey was conducted to identify Echinococcus species and other taeniids in free-roaming dogs and wild carnivores in Armenia. Fecal samples (n = 112) were opportunistically collected from eight wild carnivore species and stray dogs across six Armenian provinces between 2017 and 2018. Samples were analysed for taeniid eggs using flotation and molecular techniques. Echinococcus multilocularis was identified in a free-roaming dog, marking the first confirmed detection of the parasite in a definitive host in Armenia. Additionally, E. canadensis G6/7 and E. ortleppi were detected in a wolf, while E. canadensis G6/7, Taenia hydatigena, and T. laticollis were found in a lynx. These findings indicate the involvement of both domestic and wild carnivores in the transmission cycles of Echinococcus species and suggest the presents of a potential sylvatic cycle involving E. canadensis G6/7 and E. ortleppi. The presence of E. multilocularis in a dog underscores the risk of human infection, necessitating further epidemiological studies. This study provides initial insights into the epidemiology of Echinococcus in Armenia and highlights the need for continued surveillance to assess public health risks.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"668-676"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of the tegument of Fasciola hepatica to infection and immunization sera in vitro. 肝片形吸虫被膜对体外感染和免疫血清的反应。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S003118202510022X

Clive E Bennett, Adam P S Bennett, Robert E B Hanna, Mark W Robinson

{"title":"Response of the tegument of Fasciola hepatica to infection and immunization sera in vitro.","authors":"Clive E Bennett, Adam P S Bennett, Robert E B Hanna, Mark W Robinson","doi":"10.1017/S003118202510022X","DOIUrl":"10.1017/S003118202510022X","url":null,"abstract":"The migratory phase is a critical time for Fasciola hepatica as it must locate, penetrate and migrate through the alimentary tract to the liver parenchyma whilst under attack from the host immune response. Here, scanning and transmission electron microscopy were used to monitor the in vitro effects of sera (with, and without, complement depletion) on F. hepatica newly excysted juveniles (NEJs) and flukes recovered at 7, 35, 70 and 98 days post infection (dpi) from the liver and bile ducts of rats. Test sera were from these F. hepatica-infected rats. A F. hepatica NEJ-specific rabbit antiserum was also used. All fluke stages demonstrated release of the tegumental glycocalyx and microvesicles and intense activity within the tegumental syncytium characterized by eccrine secretion of T-0/T-1/T-2 secretory bodies with subsequent microvillar formation and shedding of microvesicles from the apical plasma membrane. Exposure of both NEJs and 35 dpi flukes to 35 and 70 dpi rat sera produced significant amounts of eccrine-derived secretory material and putative attached immunocomplex. Rabbit anti-F. hepatica NEJ-specific antiserum produced similar responses at the NEJ tegument, including binding of putative immunocomplex to the surface, but with additional blistering of some regions of the apical plasma membrane. Our data suggest that immune sera stimulates multiple interrelated secretory mechanisms to maintain the integrity of the tegumental barrier in response to immune attack. Concurrent release of microvesicles may also serve to both divert the immune response away from the fluke itself and permit delivery of immunomodulatory cargo to immune effector cells.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"697-703"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local and global density have distinct and parasite-dependent effects on infection in wild sheep. 本地和全球密度对野生羊的感染具有明显的寄生虫依赖效应。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S0031182025100383

Gregory F Albery, Amy R Sweeny, Yolanda Corripio-Miyar, Mike J Evans, Adam D Hayward, Josephine M Pemberton, Jill G Pilkington, Daniel H Nussey

{"title":"Local and global density have distinct and parasite-dependent effects on infection in wild sheep.","authors":"Gregory F Albery, Amy R Sweeny, Yolanda Corripio-Miyar, Mike J Evans, Adam D Hayward, Josephine M Pemberton, Jill G Pilkington, Daniel H Nussey","doi":"10.1017/S0031182025100383","DOIUrl":"10.1017/S0031182025100383","url":null,"abstract":"High density should drive greater parasite exposure. However, evidence linking density with infection generally uses density proxies or measures of population size, rather than measures of individuals per space within a continuous population. We used a long-term study of wild sheep to link within-population spatiotemporal variation in host density with individual parasite counts. Although four parasites exhibited strong positive relationships with local density, these relationships were mostly restricted to juveniles and faded in adults. Furthermore, one ectoparasite showed strong negative relationships across all age classes. In contrast, population size - a measure of global density - had limited explanatory power, and its effects did not remove those of spatial density, but were distinct. These results indicate that local and global density can exhibit diverse and contrasting effects on infection within populations. Spatial measures of within-population local density may provide substantial additional insight to temporal metrics based on population size, and investigating them more widely could be revealing.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"715-723"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the evolutionary pathways of Ochoterenella: a new species discovery and its phylogenetic implications. 揭示奥氏菌的进化途径：一个新物种的发现及其系统发育意义。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S0031182025100462

Gabriel Lima Rebêlo, Jorge Kevin Silva Neves, Fred Gabriel Haick, Ronald Ferreira Jesus, Karina Varella, Luiz Felipe Ferreira Trindade, Leticia de Aguiar da Costa, Fabrícia de Jesus Paiva da Fonseca Sizo, Arnaldo Maldonado Júnior, Carlos Eduardo Costa-Campos, Francisco Tiago Vasconcelos Melo

{"title":"Unveiling the evolutionary pathways of Ochoterenella: a new species discovery and its phylogenetic implications.","authors":"Gabriel Lima Rebêlo, Jorge Kevin Silva Neves, Fred Gabriel Haick, Ronald Ferreira Jesus, Karina Varella, Luiz Felipe Ferreira Trindade, Leticia de Aguiar da Costa, Fabrícia de Jesus Paiva da Fonseca Sizo, Arnaldo Maldonado Júnior, Carlos Eduardo Costa-Campos, Francisco Tiago Vasconcelos Melo","doi":"10.1017/S0031182025100462","DOIUrl":"10.1017/S0031182025100462","url":null,"abstract":"Ochoterenella is a large group of filarial parasites of anurans distributed throughout Central and South America. In the present study, we describe a new species of Ochoterenella parasitizing 2 frogs, Boana geographica and Boana multifasciata, from different localities in the Brazilian Amazon. The main morphological traits that differ Ochoterenella casiraghii n. sp. from its congeners are the smaller body size, a shorter cephalic plate, smaller parastomal structures, and the small, short and rounded cuticular bosses on the body of both sexes. The females have a shorter ovejector, and the number of caudal papillae distinguishes males. Pairwise sequence comparisons of the new species reveal a high level of divergence from Ochoterenella spp. Our phylogenetic analyses, based on cox1 and concatenated partial mitochondrial genes, support the monophyly of all subfamilies and genera examined herein. The new species represents the 17th in the Ochoterenella genus and a new parasite record for both anuran species. We provide the first ultrastructural description of the species in the genus and establish the phylogenetic relationships of the new species among parasites of amphibians and reptiles from the Onchocercidae.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"677-696"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographic variation in the determinants of ectoparasite faunas' species richness: fleas and gamasid mites parasitic on small mammals from 6 biogeographic realms. 外寄生动物群物种丰富度决定因素的地理差异：蚤和革螨寄生于6个生物地理领域的小型兽类。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S0031182025100371

Boris R Krasnov, Vasily I Grabovsky, Natalia Korallo-Vinarskaya, Maxim V Vinarski, Angel Luis Robles Fernandez, Irina Khokhlova

{"title":"Geographic variation in the determinants of ectoparasite faunas' species richness: fleas and gamasid mites parasitic on small mammals from 6 biogeographic realms.","authors":"Boris R Krasnov, Vasily I Grabovsky, Natalia Korallo-Vinarskaya, Maxim V Vinarski, Angel Luis Robles Fernandez, Irina Khokhlova","doi":"10.1017/S0031182025100371","DOIUrl":"10.1017/S0031182025100371","url":null,"abstract":"We investigated the effects of body mass, geographic range size, the within-range richness of host assemblages (diversity field) and the habitat breadth of small mammalian hosts from 6 biogeographic realms on the species richness of their flea and gamasid mite faunas. We also tested whether the probability of between-host ectoparasite sharing is related to host phylogenetic relatedness, trait similarity or geographic distance/environmental dissimilarity between their ranges. We asked whether the effects of host-associated determinants of ectoparasite richness and the probability of ectoparasite sharing differ between (1) biogeographic realms and (2) fleas and mites. Whenever significant effects of host body mass on ectoparasite richness were found, they were negative, whereas the significant effects of geographic range size, diversity field and habitat breadth were positive. The occurrence of each determinant's effects on ectoparasite species richness differed (1) within fleas or mites between realms and (2) between fleas and mites within a realm. In all realms, the probability of a flea or a mite species being shared between hosts decreased with a decrease in the hosts' phylogenetic relatedness, trait similarity, geographic distance between ranges or environmental similarity. The probabilities of an ectoparasite species being shared between hosts were most strongly related to the hosts' trait similarity and were least related to the environmental similarity. We conclude that caution is needed in making judgements about the generality of macroecological patterns related to parasites based on the investigations of these patterns in limited numbers of localities and when pooling data on various taxa.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"745-756"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and diversity of Acanthocephala in stream-dwelling amphipods (Gammarus fossarum) around an urban area in the eastern Alpine foothills. 东阿尔卑斯山麓城市地区河栖片足类（Gammarus fossarum）棘头类的流行和多样性。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S0031182025100449

Fabian Gallhammer, Jacqueline Grimm, Susanne Reier, Kristina M Sefc

{"title":"Prevalence and diversity of Acanthocephala in stream-dwelling amphipods (Gammarus fossarum) around an urban area in the eastern Alpine foothills.","authors":"Fabian Gallhammer, Jacqueline Grimm, Susanne Reier, Kristina M Sefc","doi":"10.1017/S0031182025100449","DOIUrl":"10.1017/S0031182025100449","url":null,"abstract":"Population dynamics of aquatic parasites respond to factors like host availability, habitat age and quality. Amphipods are intermediate hosts for Acanthocephala, a widespread group of parasitic worms. Acanthocephalan infections of amphipods can easily be detected, and the widespread occurrence of amphipods makes their infection status an attractive potential proxy for the ecological status of their aquatic environment, including stressors introduced by urbanization. This study investigated the prevalence and the species-level and genetic diversity of Acanthocephala in the stream amphipod Gammarus fossarum. The study streams cross forested, agricultural and urban landscapes in the eastern foothills of the European Alps. Parasite prevalence ranged from 0% to 8.8% and increased towards downstream reaches independent of surrounding land use. Oxford Nanopore Technology was used to sequence the mitochondrial cytochrome oxidase I barcoding locus to identify parasite species and assess their genetic diversity. The majority of the parasites were Pomphorhynchus tereticollis, which use fish as definitive hosts. Despite their relative abundance in the studied streams, their genetic diversity was low and the most common haplotype was found at all sampling sites, which might indicate population expansion. Amphipods also hosted P. laevis and Polymorphus sp. type 1, the first evidence of this cryptic species within Polymorphus cf. minutus in Austria. Genetic diversity was high in Polymorphus sp. type 1, possibly reflecting a large effective population size due to gene flow maintained by the avian final hosts. The low and downstream-biased prevalence suggests that definitive hosts may be a limiting factor for Acanthocephala populations in small streams.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"657-667"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidation of the life cycle of the trematode Curtuteria arguinae (Digenea: Himasthlidae), using environmental DNA detection methods. 利用环境DNA检测方法研究黄毒舌吸虫的生活史。

IF 2.4 3区医学

Parasitology Pub Date : 2025-06-01 DOI: 10.1017/S0031182025100267

Leslie Stout, Guillemine Daffe, Aurélie Chambouvet, Adrien de Montaudouin, Flore Daramy, Xavier de Montaudouin

{"title":"Elucidation of the life cycle of the trematode Curtuteria arguinae (Digenea: Himasthlidae), using environmental DNA detection methods.","authors":"Leslie Stout, Guillemine Daffe, Aurélie Chambouvet, Adrien de Montaudouin, Flore Daramy, Xavier de Montaudouin","doi":"10.1017/S0031182025100267","DOIUrl":"10.1017/S0031182025100267","url":null,"abstract":"Detection approaches based on environmental DNA (eDNA) are widely used for free-living species but remain underutilized for parasite species. This study applies eDNA detection methods to elucidate the life cycle of the trematode Curtuteria arguinae, which infects the socioeconomically and ecologically important edible cockle (Cerastoderma edule) as its second intermediate host along the northeastern Atlantic coast, including Arcachon Bay, France. The first intermediate and definitive hosts remained unknown. To identify these hosts - presumed to be a gastropod and a shorebird - we developed a quantitative PCR (qPCR)-based eDNA approach targeting partial cox1 and SSU gene regions of C. arguinae. We tested for C. arguinae eDNA presence in water samples containing separately five dominant gastropod species and fecal samples from known cockle predators, the European oystercatcher (Haematopus ostralegus) and gulls (Larus spp.), collected in Arcachon Bay. C. arguinae eDNA was only detected in water containing the needle snail (Bittium reticulatum), with cercarial emergence confirming infection in 1.6% of individual hosts. Morphological analysis of the cercarial and metacercarial stages revealed variability in collar spine visibility. Additionally, C. arguinae was detected by qPCR in 42% of oystercatcher feces and no gull feces, suggesting oystercatchers are the definitive host. This study is the first to elucidate the complete life cycle of C. arguinae, identifying B. reticulatum as its first intermediate host and H. ostralegus as its definitive host. Our findings highlight the potential of eDNA approaches for resolving parasite life cycles and enabling advances in ecological research on C. arguinae.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"704-714"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0