Parasitology最新文献_第9页

Do specialist haemoparasites induce tolerance in their hosts? 专科血寄生虫会诱导宿主产生耐受性吗？

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000393

Cameron Armour, Abigail L Hone, Jenny C Dunn

引用次数: 0

Characterization and microRNA quantification of plasma-derived extracellular vesicles in patients with Plasmodium knowlesi infection. 诺氏疟原虫感染患者血浆源性细胞外囊泡的表征和MicroRNA定量分析。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000423

Thunchanok Khammanee, Natakorn Nokchan, Piyatida Molika, Hansuk Buncherd, Kanitta Srinoun, Natta Tansila, Charinrat Saechan, Rachanida Praparatana, Nongyao Sawangjaroen, Suwannee Jitueakul, Chatree Ratcha, Churat Weeraphan, Jisnuson Svasti, Raphatphorn Navakanitworakul, Supinya Thanapongpichat

{"title":"Characterization and microRNA quantification of plasma-derived extracellular vesicles in patients with Plasmodium knowlesi infection.","authors":"Thunchanok Khammanee, Natakorn Nokchan, Piyatida Molika, Hansuk Buncherd, Kanitta Srinoun, Natta Tansila, Charinrat Saechan, Rachanida Praparatana, Nongyao Sawangjaroen, Suwannee Jitueakul, Chatree Ratcha, Churat Weeraphan, Jisnuson Svasti, Raphatphorn Navakanitworakul, Supinya Thanapongpichat","doi":"10.1017/S0031182025000423","DOIUrl":"10.1017/S0031182025000423","url":null,"abstract":"MicroRNAs (miRNAs), derived from extracellular vesicles (EVs) are circulating intercellular communicators which influence pathogenesis and could be used as potential diagnostic markers. In this study, plasma-derived EVs from Plasmodium knowlesi-infected patients (n = 13) and healthy individuals (n = 10) were isolated using size exclusion chromatography and ultracentrifugation. The presence of EVs was confirmed by transmission electron microscopy and Western immunoblotting, and quantified by nanoparticle tracking analysis. The EVs isolated from patients exhibited a larger size, accompanied by an elevated concentration of EVs. The relative expression levels of 8 human miRNAs were quantified using reverse transcriptase quantitative polymerase chain reaction. Compared to uninfected groups, hsa-miR-223-5p (P-value = 0.0002) and hsa-miR-486-5p (P-value = 0.025) were upregulated in P. knowlesi-infected patients. Bioinformatic analysis revealed that these miRNAs are predicted to target both human host and parasite genes, and they were found to be enriched in various malaria-related pathways. The areas under the receiver operating characteristic curve of hsa-miR-223-5p and hsa-miR-486-5p were 0.9154 and 0.8231, respectively, suggesting the potential of EV-miRNAs as diagnostic markers. Results revealed that EV-miRNAs may play a significant role in the progression of P. knowlesi infection. Further investigations should explore their potential impact on gene expression regulation as diagnostic biomarkers or targets for therapeutic interventions.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"381-394"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Spirometra isolates across the USA. 美国肺metra分离株的分子特征。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S003118202500054X

Tiana L Sanders, Caroline Sobotyk, Pablo D Jimenez Castro, Amira Abdu, Jennifer Baade, Mindy Borst, Sriveny Dangoudoubiyam, Brooke A Delcambre, Jeff M Gruntmeir, Alice Lee, Christian Leutenegger, Cecilia Lozoya, Gleeson Murphy, Cassan Pulaski, John Schaefer, Adriano Vatta, Heather D S Walden, Manigandan Lejeune, Guilherme G Verocai

{"title":"Molecular characterization of Spirometra isolates across the USA.","authors":"Tiana L Sanders, Caroline Sobotyk, Pablo D Jimenez Castro, Amira Abdu, Jennifer Baade, Mindy Borst, Sriveny Dangoudoubiyam, Brooke A Delcambre, Jeff M Gruntmeir, Alice Lee, Christian Leutenegger, Cecilia Lozoya, Gleeson Murphy, Cassan Pulaski, John Schaefer, Adriano Vatta, Heather D S Walden, Manigandan Lejeune, Guilherme G Verocai","doi":"10.1017/S003118202500054X","DOIUrl":"10.1017/S003118202500054X","url":null,"abstract":"Spirometra is a genus of zoonotic cestodes with an ambiguous species-level taxonomic history. Previously, Spirometra mansonoides was considered the only species present in North America. However, recent molecular data revealed the presence of at least three distinct species in the USA: Spirometra sp. 2 and 3, and Spirometra mansoni. This study aimed to elucidate the diversity and potential host associations of Spirometra species among companion animals in the USA. Samples (N = 302) were examined from at least 13 host species, including mammals, amphibians and reptiles. Sample types included eggs isolated from faeces (n = 222), adult specimens (n = 71) and plerocercoids (n = 9) from 18 different states and 2 territories across the USA. Extracted genomic DNA was subjected to PCR targeting a fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. Generated sequences (n = 136) were included in a phylogenetic analysis. Spirometra mansoni was detected in domestic cats (n = 76), dogs (n = 12), a White's tree frog (n = 1), a Cuban knight anole (n = 1), a green iguana (n = 1) and a serval (n = 1) across 15 states and Puerto Rico. Spirometra sp. 2 was found only in dogs (n = 3) from Florida and Spirometra sp. 3 was found only in cats (n = 41) from 17 states. All plerocercoid samples were consistent with S. mansoni. The results confirm that at least three distinct Spirometra species are present and established in companion animals, such as dogs and cats, and likely are using various native and exotic species as paratenic hosts within the USA.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"477-486"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zoogonids (trematoda) infecting Indo-West Pacific damselfishes (Pomacentridae), including the proposal of a new genus and two new species. 感染印度-西太平洋豆娘鱼（豆娘科）的虫纲动物（吸虫纲），包括一个新属和两个新种的建议。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000307

Berilin Duong, Storm B Martin, Scott C Cutmore, Thomas H Cribb

{"title":"Zoogonids (trematoda) infecting Indo-West Pacific damselfishes (Pomacentridae), including the proposal of a new genus and two new species.","authors":"Berilin Duong, Storm B Martin, Scott C Cutmore, Thomas H Cribb","doi":"10.1017/S0031182025000307","DOIUrl":"10.1017/S0031182025000307","url":null,"abstract":"As part of a broad survey of the trematodes of damselfishes (Pomacentridae) in the tropical Indo-West Pacific, zoogonids were collected from multiple localities in Australia, New Caledonia, and French Polynesia. All zoogonid specimens collected were consistent with the subfamily Lecithostaphylinae, and morphological and molecular data (ITS2 and 28S rDNA, and cox1 mtDNA) were generated for most host-locality combinations to enable an integrative species delimitation. The collection comprised three species: Deretrema stratiotes n. sp. from four species of Abudefduf Forsskål from Ningaloo Reef in Western Australia, and two species consistent with the genus Lecithostaphylus Odhner, 1911 for which Innuptacola n. gen. is proposed based on phylogenetic and morphological distinction, the type-species I. gibsoni (Cribb, Bray & Barker, 1992) n. comb. (= L. gibsoni) from six species of Abudefduf in Ningaloo Reef, Queensland and New Caledonia, and I. torquata n. sp. from 12 pomacentrid species in Ningaloo Reef, the Great Barrier Reef in Queensland, and the Gambier Islands in French Polynesia. The new collection demonstrates that some zoogonid species are geographically widespread (from the Pacific Ocean to the Indian Ocean) and can infect a broad range of hosts (multiple genera within a family), whereas others are apparently geographically restricted and exhibit higher host-specificity (fishes within a single genus).","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"436-452"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering human Dirofilaria repens infections: new cases in Southern Italy. 发现人间双丝虫感染：意大利南部新病例。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000290

Lavinia Ciuca, Simona Gabrielli, Patrizia Forgione, Evaristo Di Napoli, Orlando Paciello, Maria Vittoria Panariello, Marianna Ascierto, Luciana Petrullo, Maria Ortensia Montella, Maria Paola Maurelli, Laura Rinaldi

引用次数: 0

Helminths assemblage in two opossum's species, Didelphis albiventris and Didelphis aurita (Mammalia: Didelphimorphia), from the Atlantic Forest of Argentina. 阿根廷大西洋森林中两种负鼠的蠕虫群，白腹负鼠和aurita负鼠（哺乳纲：负鼠）。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000563

Bárbara Betina Hartmann, Ezequiel Andres Vanderhoeven, Dante Luis Di Nucci, Agustín Solari, Juliana Notarnicola

{"title":"Helminths assemblage in two opossum's species, Didelphis albiventris and Didelphis aurita (Mammalia: Didelphimorphia), from the Atlantic Forest of Argentina.","authors":"Bárbara Betina Hartmann, Ezequiel Andres Vanderhoeven, Dante Luis Di Nucci, Agustín Solari, Juliana Notarnicola","doi":"10.1017/S0031182025000563","DOIUrl":"10.1017/S0031182025000563","url":null,"abstract":"In the Argentinian Atlantic Forest (AAF) of Misiones, opossums comprise 13% of the wild mammalian diversity. The white-eared opossum, Didelphis albiventris, and the southern black-eared opossum, D. aurita are sympatric marsupials, and the most frequent mammals in the northern Misiones. In this study, we describe the helminth assemblages from both D. albiventris and D. aurita in the northern AAF. We found a total of 15 species of helminths: 2 trematodes, 1 cestode, 11 nematodes and 1 acanthocephalan. The specific richness in D. albiventris was 12, while in D. aurita was it 13. Both opossum's species share 10 helminth species; D. albiventris presented Capillaria sp. 2 and Globocephalus marsupials, absent in D. aurita; while D. aurita presented Trichuris didelphis, Capillaria sp. 1, and Travassostrongylus orloffi, absent in D. albiventris. Cruzia tentaculata registered the highest prevalence in both opossum species. Seven out of the 12 helminth species identified in D. albiventris have an indirect life cycle. Similarly, in D. aurita, 5 out of 13 helminth species exhibit an indirect life cycle. This suggests that nearly half of the assemblage of helminth in both opossum species need an intermediate host acquired through the diet. We also present new records for Argentina including Trichuris minuta, G. marsupialis, Viannaia viannai, T. orloffi and T. callis. This is the first time the helminth assemblage has been described for D. aurita in Argentina.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"551-562"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and molecular characterization of the trematodes (Digenea: Acanthocolpidae and Cryptogonimidae) of the black-spotted croaker (Protonibea diacanthus) (Teleostei: Sciaenidae) in northern Australia. 澳洲北部黑斑鱼吸虫的形态和分子特征（Digenea：棘虫科和隐虫科）。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000502

Megan Porter, Diane P Barton, Xiaocheng Zhu, Shokoofeh Shamsi

引用次数: 0

Molecular analysis of Sarcoptes scabiei infecting wild and domestic South American camelids in Argentina. 阿根廷野生和家养南美骆驼感染疥螨的分子分析。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000344

Melina Anello, Fabiana Sosa, Hebe Ferreyra, Rebeca Lobo Allende, Mariana Mastromatey, Marcela Uhart, Sandra Romero, Mónica Florin-Christensen, Barbara Moroni, Anna Rita Molinar, Luca Rossi, Florencia Di Rocco

{"title":"Molecular analysis of Sarcoptes scabiei infecting wild and domestic South American camelids in Argentina.","authors":"Melina Anello, Fabiana Sosa, Hebe Ferreyra, Rebeca Lobo Allende, Mariana Mastromatey, Marcela Uhart, Sandra Romero, Mónica Florin-Christensen, Barbara Moroni, Anna Rita Molinar, Luca Rossi, Florencia Di Rocco","doi":"10.1017/S0031182025000344","DOIUrl":"10.1017/S0031182025000344","url":null,"abstract":"Sarcoptic mange, caused by the Sarcoptes scabiei mite, is a highly transmissible skin condition affecting many mammalian species worldwide. South American camelids (SAC) have the highest reported prevalence of mange in South America, causing economic losses and posing a conservation threat to wild SAC. This study investigated mite diversity in SAC in Argentina and assessed relationships between known outbreak areas. Distinct epidemiologic scenarios were explored: the San Juan-La Rioja region, where a mange outbreak decimated wild SAC populations, and the Puna region of Jujuy, where domestic and wild SAC coexist and infections often occur. The mitochondrial gene cox1 and ten microsatellites were analysed from mites collected in five sampling events in Jujuy and four in San Juan-La Rioja between 2017 and 2023. A single cox1 haplotype was observed regardless of mite origin or host species. Comparison with partial cox1 sequences from other camelids worldwide showed little variation. Microsatellite markers revealed lower diversity in mites from San Juan-La Rioja compared to Jujuy. A single strain common to vicuñas and guanacos was identified in San Juan-La Rioja, while three strains were detected in Jujuy affecting vicuñas and/or domestic llamas. Some mites from Jujuy exhibited mixed genetic composition between the two regions, and results confirmed that domestic and wild SAC shared mite strains. This study enhances understanding of sarcoptic mange transmission among SAC species, contributing to vicuña and guanaco conservation and high-altitude livestock farming. Additionally, these findings provide support for the development of intersectoral management strategies to address this significant threat.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"409-418"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection with Angiostrongylus cantonensis reveals up- and down-regulation of the protein profile in the mucus of infected slugs. 广州管圆线虫感染后，感染鼻涕虫黏液中的蛋白谱出现了上调和下调。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000435

Joana Borges Osório, Giovanni Gosch Berton, Leandro Mattos Pereira, Jeremy Potriquet, Renata Russo F Cândido, Jason Mulvenna, Malcolm K Jones, Carlos Graeff-Teixeira, Alessandra Loureiro Morassutti

{"title":"Infection with Angiostrongylus cantonensis reveals up- and down-regulation of the protein profile in the mucus of infected slugs.","authors":"Joana Borges Osório, Giovanni Gosch Berton, Leandro Mattos Pereira, Jeremy Potriquet, Renata Russo F Cândido, Jason Mulvenna, Malcolm K Jones, Carlos Graeff-Teixeira, Alessandra Loureiro Morassutti","doi":"10.1017/S0031182025000435","DOIUrl":"10.1017/S0031182025000435","url":null,"abstract":"Host-parasite adaptation is crucial for evolutionary success of a parasite. The nematode Angiostrongylus cantonensis has a heterogenic life cycle involving molluscs as intermediate hosts and rats as definitive hosts. Several mollusc species are susceptible hosts of A. cantonensis, allowing the development of first-stage larvae (L1) into third-stage larvae (L3). Changes in the metabolism of infected molluscs have been demonstrated, disturbing regular routes and inducing host defence mechanisms. This study aimed to identify changes in the proteomic content of Phyllocaulis spp. mucus during A. cantonensis infection. Proteins were extracted from the mucus samples of infected and non-infected slugs and identified using liquid chromatography-tandem mass spectrometry. We found 26 up-regulated and 15 down-regulated proteins in infected slugs compared to non-infected slugs. Protein profiles are promising markers of parasite infection, and a better understanding of proteomic profiles during infection may help inform in vivo studies and promote new tools for the non-invasive identification of infected hosts.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"395-398"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A first report of the detection of Avipoxvirus genomic sequences in louse flies (Diptera: Hippoboscidae). 蝇类中蝇痘病毒基因组序列检测的首次报道（双翅目：蝇科）。

IF 2.1 3区医学

Parasitology Pub Date : 2025-04-01 DOI: 10.1017/S0031182025000526

Denise Wawman, Ben P Jones, Steven R Fiddaman, Jane E Turner, Nicholas Johnson, Adrian L Smith

{"title":"A first report of the detection of Avipoxvirus genomic sequences in louse flies (Diptera: Hippoboscidae).","authors":"Denise Wawman, Ben P Jones, Steven R Fiddaman, Jane E Turner, Nicholas Johnson, Adrian L Smith","doi":"10.1017/S0031182025000526","DOIUrl":"10.1017/S0031182025000526","url":null,"abstract":"The Hippoboscidae are ectoparasites of birds and mammals, which, as a group, are known to vector multiple diseases. Avipoxvirus (APV) is mechanically vectored by various arthropods and causes seasonal disease in wild birds in the United Kingdom (UK). Signs of APV and the presence of louse flies (Hippoboscidae) on Dunnocks Prunella modularis were recorded over a 16·5-year period in a rural garden in Somerset, UK. Louse flies collected from this site and other sites in England were tested for the presence of APV DNA and RNA sequences. Louse flies on Dunnocks were seen to peak seasonally three weeks prior to the peak of APV lesions, an interval consistent with the previously estimated incubation period of APV in Dunnocks. APV DNA was detected on 13/25 louse flies, Ornithomya avicularia and Ornithomya fringillina, taken from Dunnocks, both with and without lesions consistent with APV, at multiple sites in England. Collectively these data support the premise that louse flies may vector APV. The detection of APV in louse flies, from apparently healthy birds, and from sites where disease has not been observed in any host species, suggests that the Hippoboscidae could provide a non-invasive and relatively cheap method of monitoring avian diseases. This could provide advanced warnings of disease, including zoonoses, before they become clinically apparent.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"522-530"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0