Parasitology最新文献_第3页

Host genotype and infection status interact to shape microbiomes in Daphnia magna. 宿主基因型和感染状态相互作用，形成大型蚤体内的微生物群。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-15 DOI: 10.1017/S0031182024000787

Amruta Rajarajan, Ellen Decaestecker, Lore Bulteel, Jean-Claude Walser, Piet Spaak, Justyna Wolinska

{"title":"Host genotype and infection status interact to shape microbiomes in Daphnia magna.","authors":"Amruta Rajarajan, Ellen Decaestecker, Lore Bulteel, Jean-Claude Walser, Piet Spaak, Justyna Wolinska","doi":"10.1017/S0031182024000787","DOIUrl":"https://doi.org/10.1017/S0031182024000787","url":null,"abstract":"Host–bacterial communities (microbiomes) are influenced by a wide range of factors including host genotype and parasite exposure. However, few studies disentangle temporal and host-genotype-specific variation in microbiome response to infection across several host tissues. We experimentally exposed the freshwater crustacean Daphnia magna to its fungal parasite Metschnikowia bicuspidata and characterized changes in host–bacterial communities associated with the parasite's development within the host. We used 16S rRNA gene sequencing to assess bacterial communities of the host (a) 24 h (‘initial parasite exposure’) and (b) 10 days (‘successful infection’) after exposure to a standard dose of M. bicuspidata spores, in host guts, body tissue (excluding guts) and whole individuals. We also investigated whether bacterial community responses to parasite exposure varied by host genotype.Parasite exposure did not immediately alter host gut bacterial communities, but drove host-genotype-specific changes in the bacterial community composition of whole individuals. We validated that these changes were not driven by shifts in bacterial communities of the culturing medium, due to the addition of the parasite spore solution. Successful infection (i.e. the proliferation of M. bicuspidata spores in the host body) reduced alpha diversity and shifted abundance of dominant bacterial orders in the gut. Moreover, it induced a host-genotype-specific changes in body bacterial community composition. Overall, bacterial community responses to parasite exposure and subsequent infection are complex: they occur in a host-genotype-dependent manner, differentially at distinct timepoints after parasite exposure, and in specific host tissue.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitogenomics of the zoonotic parasite Echinostoma miyagawai and insights into the evolution of tandem repeat regions within the mitochondrial non-coding control region. 人畜共患病寄生虫 Echinostoma miyagawai 的有丝分裂基因组学以及线粒体非编码控制区串联重复区进化的启示。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-14 DOI: 10.1017/S0031182024001422

Linh Thi Khanh Pham, Dong Van Quyen, Weerachai Saijuntha, Huong Thi Thanh Doan, Thanh Hoa Le, Scott P Lawton

引用次数: 0

Ecological relationships of Haemaphysalis longicornis Neumann with other tick species on wildlife hosts at cow-calf farms implementing integrated pest management in eastern Tennessee. 田纳西州东部实施虫害综合防治的奶牛-小牛农场中，Haemaphysalis longicornis Neumann 与其他蜱虫物种在野生动物宿主身上的生态关系。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-14 DOI: 10.1017/S0031182024001380

Rebecca A Butler, Lisa I Muller, Dan Grove, R T Trout Fryxell

引用次数: 0

Molecular characterization and B-cell epitope analysis of the TSP11 gene in Echinococcus infection strains from Yunnan Province. 云南省棘球蚴感染株 TSP11 基因的分子特征和 B 细胞表位分析。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-12 DOI: 10.1017/S0031182024000726

Qian Xu, Zhengqing Wang, Jinrong Zi, Xuan Cai, Fangwei Wu, Benfu Li, Jia Peng, Jianxiong Li, Xinliu Yan, Ying Dong, Yaming Yang

{"title":"Molecular characterization and B-cell epitope analysis of the TSP11 gene in Echinococcus infection strains from Yunnan Province.","authors":"Qian Xu, Zhengqing Wang, Jinrong Zi, Xuan Cai, Fangwei Wu, Benfu Li, Jia Peng, Jianxiong Li, Xinliu Yan, Ying Dong, Yaming Yang","doi":"10.1017/S0031182024000726","DOIUrl":"https://doi.org/10.1017/S0031182024000726","url":null,"abstract":"This study investigates the molecular intricacies of the transmembrane protein TSP11 gene in Echinococcus strains isolated from livestock and patients in Yunnan Province afflicted with Echinococcus granulosus (E. granulosus) between 2016 and 2020. Gene typing analysis of the ND1 gene revealed the presence of the G1 type, G5 type and untyped strains, constituting 52.4, 38.1 and 9.5%, respectively. The analysis of 42 DNA sequences has revealed 24 novel single nucleotide polymorphic sites, delineating 11 haplotypes, all of which were of the mutant type. Importantly, there were no variations observed in mutation sites or haplotypes in any of the hosts. The total length of the TSP11 gene's 4 exons is 762 bp, encoding 254 amino acids. Our analysis posits the existence of 6 potential B-cell antigenic epitopes within TSP11, specifically at positions 49-KSN-51, 139-GKRG-142, 162-DNG-164, 169-NGS-171, 185-DS-186 and 231-PPRFTN-236. Notably, these epitopes exhibit consistent presence among various intermediate hosts and haplotypes. However, further validation is imperative to ascertain their viability as diagnostic antigens for E. granulosus in the Yunnan Province.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nest secondary plants and their associations with haemosporidian blood parasites in blue tit females. 蓝山雀雌鸟巢中的次生植物及其与血孢子虫血液寄生虫的关系。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-12 DOI: 10.1017/S0031182024000775

Jorge García-Campa, Sonia González-Braojos, Judith Morales

{"title":"Nest secondary plants and their associations with haemosporidian blood parasites in blue tit females.","authors":"Jorge García-Campa, Sonia González-Braojos, Judith Morales","doi":"10.1017/S0031182024000775","DOIUrl":"https://doi.org/10.1017/S0031182024000775","url":null,"abstract":"Avian nests often contain aromatic plant fragments, which has led to propose among others that they repel ectoparasites or vectors of blood parasites (‘nest protection hypothesis’). To date, the relationship between secondary plant provisioning and the parent's blood parasites remains unexplored. We investigated whether the presence of secondary plants in nests during different reproductive stages (before incubation, during incubation and nestling period) was associated with the presence of nest-dwelling ectoparasites and females’ blood-parasite infections in blue tits (Cyanistes caeruleus) during chick rearing. In this species, females are highly exposed to parasites, particularly at the beginning of the breeding season, since they build the nest and incubate alone. They also brood the nestlings while the male assists with provisioning. We found that females that provided fewer plants before incubation were more likely to be infected by Plasmodium. Specifically, Females that did not provide Achillea herbs before incubation were more likely to be infected by both Plasmodium and Haemoproteus, reinforcing the ‘nest protection’ hypothesis. Thus, secondary plants may create an early environment that masks the odour of hosts or repels vectors. Surprisingly, the presence of Lavandula during the nestling period was positively related to Leucocytozoon infection. Given its fastest development among haemosporidians, we speculate that Lavandula herbs are provided by females to reduce mother-to-offspring transmission or as a self-medication strategy. Finally, the number of plant fragments provided before incubation was negatively associated with the number of (Protocalliphora azurea) larvae, whereas there were no associations with the presence of mites (Dermanyssus gallinoides).","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suitability of invasive gobies as paratenic hosts for acanthocephalans of the genus Pomphorhynchus sp. 外来虾虎鱼作为庞氏棘尾鱼属副宿主的适宜性

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-12 DOI: 10.1017/S0031182024001197

Milen Nachev, Michael Hohenadler, Nicklas Bröckers, Daniel Grabner, Bernd Sures

{"title":"Suitability of invasive gobies as paratenic hosts for acanthocephalans of the genus Pomphorhynchus sp.","authors":"Milen Nachev, Michael Hohenadler, Nicklas Bröckers, Daniel Grabner, Bernd Sures","doi":"10.1017/S0031182024001197","DOIUrl":"https://doi.org/10.1017/S0031182024001197","url":null,"abstract":"Ponto-Caspian gobies became highly abundant in many regions outside their native distribution range (e.g. in the Rhine River system). In the newly invaded habitats, the parasite communities of the invasive gobies are characterized by a lower species richness compared to their native range. Interestingly, acanthocephalans of the genus Pomphorhynchus are highly abundant, although they do not become mature and mostly remain encapsulated in the abdominal cavity as preadults. Thus, gobiids could either represent a dead-end host for Pomphorhynchus sp. declining its population (dilution effect) or act as a paratenic host that could increase the infection pressure if the infected gobies are preyed upon by appropriate definitive hosts (spill back). To determine which of the 2 scenarios the gobiids contribute to, we conducted 2 infection experiments using smaller and larger individuals of the definitive host chub (Squalius cephalus), infected with preadults of Pomphorhynchus sp. collected from the abdominal cavity of Neogobius melanostomus. The results showed that preadults were able to complete their development and mature in the definitive host with mean recovery rates of 17.9% in smaller and 27.0% in larger chubs. Successful infections were observed in 62.0% and 80.0% of the smaller and larger chubs, respectively. Our study demonstrated that gobies can theoretically serve as a paratenic host for acanthocephalans of the genus Pomphorhynchus, and that infection might spill back into the local fish community if infected gobies are preyed upon by suitable definitive hosts of Pomphorhynchus sp. such as large barbel or chub.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-8"},"PeriodicalIF":2.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of CD1a immunostaining in the diagnosis of cutaneous leishmaniasis caused by Leishmania donovani in Sri Lanka. 评估 CD1a 免疫染色法在诊断斯里兰卡由多诺万利什曼病引起的皮肤利什曼病中的应用。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-11 DOI: 10.1017/S0031182024000799

Hasna Riyal, Nilakshi Samaranayake, Priyani Amarathunga, Deepani Munidasa, Nadira Karunaweera

{"title":"Evaluation of CD1a immunostaining in the diagnosis of cutaneous leishmaniasis caused by Leishmania donovani in Sri Lanka.","authors":"Hasna Riyal, Nilakshi Samaranayake, Priyani Amarathunga, Deepani Munidasa, Nadira Karunaweera","doi":"10.1017/S0031182024000799","DOIUrl":"https://doi.org/10.1017/S0031182024000799","url":null,"abstract":"Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease, routinely diagnosed by direct light microscopy. The sensitivity of this method is dependent on the number of parasites present in the lesion. Immunoexpression of CD1a surface antigen by Leishmania amastigotes and its application as a diagnostic tool has been recently demonstrated in several species including Leishmania major, Leishmania tropica and Leishmania infantum. Leishmania donovani is the only reported species in Sri Lanka primarily causing CL and its CD1a status remains unexplored. We studied CD1a expression by amastigotes of L. donovani in skin biopsies from 116 patients with suspected CL. The biopsy sections were stained with CD1a clones O10 and MTB1 separately. Slit skin smear (SSS) results were considered the gold standard for diagnosis of CL. 103 cases were confirmed through SSS where 73 of them showed positive parasite staining for CD1a clone MTB1 with 70.9% sensitivity. Positivity was seen mostly in parasites closer to the epidermis. CD1a clone O10 failed to detect any amastigotes. Test sensitivity improved to 74.1% when the analysis was applied only to patients with low/no discernible Leishman-Donovan (LD) bodies in histology. Our findings show that CD1a clone MTB1 successfully stains amastigotes of L. donovani species and can be used as a supplementary diagnostic tool in detecting CL, especially when LD bodies are low in number. This method could be validated to detect other forms of leishmaniasis caused by L. donovani in Indian and sub-Saharan regions.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-6"},"PeriodicalIF":2.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the diversity of Posthodiplostomum Dubois, 1936 (Trematoda: Diplostomidae) in fish-eating birds from the Neotropical region of Mexico, with the description of a new species. 揭示墨西哥新热带地区食鱼鸟类中 Posthodiplostomum Dubois, 1936 (Trematoda: Diplostomidae) 的多样性，并描述一个新物种。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-11 DOI: 10.1017/S0031182024000970

Marcelo Tonatiuh González-García, Alejandra López-Jiménez, Mirza Patricia Ortega-Olivares, Ana Lucia Sereno-Uribe, Gerardo Pérez-Ponce de León, Martín García-Varela

{"title":"Unravelling the diversity of Posthodiplostomum Dubois, 1936 (Trematoda: Diplostomidae) in fish-eating birds from the Neotropical region of Mexico, with the description of a new species.","authors":"Marcelo Tonatiuh González-García, Alejandra López-Jiménez, Mirza Patricia Ortega-Olivares, Ana Lucia Sereno-Uribe, Gerardo Pérez-Ponce de León, Martín García-Varela","doi":"10.1017/S0031182024000970","DOIUrl":"https://doi.org/10.1017/S0031182024000970","url":null,"abstract":"Adults of the genus Posthodiplostomum, Dubois, 1936 are parasites of fish-eating birds, mainly of the family Ardeidae, and are globally distributed. The genus currently comprises 35 species, although recent molecular evidence has shown that the diversity of the genus is underestimated since several candidate species have been recognized. In the Neotropical region of Mexico, at least 6 Posthodiplostomum lineages have been detected with metacercaria stages recovered from unrelated fish hosts. Here, we obtained adult specimens of Posthodiplostomum from 6 fish-eating birds representing 2 families (Butorides virescens, Ardea herodias, Nycticorax nycticorax, Tigrisoma mexicanum – Ardeidae, and Rynchops niger and Leucophaeus atricilla – Lariidae) from 4 localities in southern Mexico. Specimens were sequenced for 2 nuclear (28S and ITS1–5.8S–ITS2) and 1 mitochondrial (cox1) molecular marker. Phylogenetic analyses allowed us to link metacercariae and adult specimens and recognized a lineage, which was described morphologically. The new species can be distinguished from its congeners by its prosoma morphology and body size; this is the first described species in the Neotropical region of Mexico. Additionally, new host and locality records for P. macrocotyle and P. pricei are presented, expanding their geographical distribution range in the Americas.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-17"},"PeriodicalIF":2.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyses of the essential C82 subunit uncovered some differences in RNA polymerase III transcription between Trypanosoma brucei and Leishmania major. 对重要的 C82 亚基的分析发现，布氏锥虫和大利什曼原虫的 RNA 聚合酶 III 转录存在一些差异。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-11 DOI: 10.1017/S0031182024000921

Andrés Cano-Santiago, Luis E Florencio-Martínez, Daniel E Vélez-Ramírez, Adrián J Romero-Chaveste, Rebeca G Manning-Cela, Tomás Nepomuceno-Mejía, Santiago Martínez-Calvillo

{"title":"Analyses of the essential C82 subunit uncovered some differences in RNA polymerase III transcription between Trypanosoma brucei and Leishmania major.","authors":"Andrés Cano-Santiago, Luis E Florencio-Martínez, Daniel E Vélez-Ramírez, Adrián J Romero-Chaveste, Rebeca G Manning-Cela, Tomás Nepomuceno-Mejía, Santiago Martínez-Calvillo","doi":"10.1017/S0031182024000921","DOIUrl":"https://doi.org/10.1017/S0031182024000921","url":null,"abstract":"The 17-subunit RNA polymerase III (RNAP III) synthesizes essential untranslated RNAs such as tRNAs and 5S rRNA. In yeast and vertebrates, subunit C82 forms a stable subcomplex with C34 and C31 that is necessary for promoter-specific transcription initiation. Little is known about RNAP III transcription in trypanosomatid parasites. To narrow this knowledge gap, we characterized the C82 subunit in Trypanosoma brucei and Leishmania major. Bioinformatic analyses showed that the 4 distinctive extended winged-helix (eWH) domains and the coiled-coil motif are present in C82 in these microorganisms. Nevertheless, C82 in trypanosomatids presents certain unique traits, including an exclusive loop within the eWH1 domain. We found that C82 localizes to the nucleus and binds to RNAP III-dependent genes in the insect stages of both parasites. Knock-down of C82 by RNA interference significantly reduced the levels of tRNAs and 5S rRNA and led to the death of procyclic forms of T. brucei. Tandem affinity purifications with both parasites allowed the identification of several C82-interacting partners, including C34 and some genus-specific putative regulators of transcription. However, the orthologue of C31 was not found in trypanosomatids. Interestingly, our data suggest a strong association of C82 with TFIIIC subunits in T. brucei, but not in L. major.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-16"},"PeriodicalIF":2.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Henneguya patriciai n. sp. (Cnidaria: Myxosporea) parasitizing Leporinus friderici (Bloch 1794) from Tartarugalzinho river, eastern Amazon. Henneguya patriciai n. sp. (Cnidaria: Myxosporea) 寄生于亚马逊东部 Tartarugalzinho 河的 Leporinus friderici (Bloch 1794)。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-11 DOI: 10.1017/S0031182024000684

Abthyllane Amaral de Carvalho, Lilia Suzane de Oliveira Nascimento, Luize Cristine Pantoja Dos Reis, Roger Leomar da Silva Ferreira, Saturo Cardoso Morais, Elane Guerreiro Geise, Marcela Nunes Videira, Edilson Rodrigues Matos

{"title":"Henneguya patriciai n. sp. (Cnidaria: Myxosporea) parasitizing Leporinus friderici (Bloch 1794) from Tartarugalzinho river, eastern Amazon.","authors":"Abthyllane Amaral de Carvalho, Lilia Suzane de Oliveira Nascimento, Luize Cristine Pantoja Dos Reis, Roger Leomar da Silva Ferreira, Saturo Cardoso Morais, Elane Guerreiro Geise, Marcela Nunes Videira, Edilson Rodrigues Matos","doi":"10.1017/S0031182024000684","DOIUrl":"https://doi.org/10.1017/S0031182024000684","url":null,"abstract":"The Amazon basin has the largest number of fish in the world, and among the most common fishes of the Neotropical region, the threespot (Leporinus friderici) is cited, which in relation to its microparasitic fauna, has described only 1 species of the genus Henneguya, Henneguya friderici. The Myxozoa class is considered an obligate parasite, being morphologically characterized by spores formed by valves connected by a suture line. This study describes a new species of Henneguya sp. in the Amazon region for L. friderici. This parasite was found in the host's pyloric caeca and caudal kidney, with mature spores with a total spore length of 38.4 ± 2.5 (35.9–40.9) μm; the spore body 14.4 ± 1.1 (13.3–15.5) μm and 7.3 ± 0.6 (6.7–7.9) μm wide. Regarding its 2 polar capsules, they had a length of 5.1 ± 0.4 (4.7–5.5) μm and a width of 2.0 ± 0.1 (1.9–2.1) μm in the same pear-shaped, and each polar capsule contained 9–11 turns. Morphological and phylogenetic analyses denote that this is a new species of the genus Henneguya.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-8"},"PeriodicalIF":2.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0