New genetic variants of Toxoplasma gondii isolates and a novel ROP5 allele in free-range chickens from Tabasco, México.

IF 2.4 3区医学 Q2 PARASITOLOGY

Parasitology Pub Date : 2025-07-01 DOI:10.1017/S0031182025100401

Luis Fernando Valenzuela-Moreno, Carlos Cedillo-Peláez, Claudia Patricia Rico-Torres, Lluvia Guadalupe Moreno-Pérez, Claudia Virginia Zaragoza-Vera, Luz Belinda Ortiz-Alegría, Irma Cañedo-Solares, Lizbeth Xicoténcatl-García, Fernando García-Lacy, Heriberto Caballero-Ortega

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引用次数: 0

Abstract

Toxoplasma gondii is a protozoan parasite that causes infection in birds and mammals (terrestrial and marine), both domestic and wild. The state of Tabasco has favourable climatic and ecological conditions for the replication and dissemination of this parasite. Therefore, the aim of this study was to isolate and genotype T. gondii from free-range chickens in this region of México by PCR-RFLP of 15 genetic markers. A total of 12 chickens were obtained from 7 municipalities. Serological survey by the modified agglutination test (MAT) of chicken serum samples revealed that 9 out of the 12 chickens (75%) tested had antibodies against T. gondii (titres ≥ 1:10). Toxoplasma gondii DNA was detected by PCR in tissues from 8 out of the 12 chickens. Twelve viable strains of T. gondii were isolated from the heart and brain samples of eight chickens by bioassay in mice. Genetic characterization of tachyzoite-derived DNA was performed using 10 multilocus RFLP-PCR genotyping markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) and five virulence-related markers (CS3, ROP5, ROP16, ROP17 and ROP18). A total of 6 ToxoDB PCR-RFLP genotypes were identified, including #8 (also known as Type BrIII), #28, #38, and 3 new genotypes designated as #344, #345 and #346. Combination of ROP18/ROP5 alleles were 1/3, 3/3 and 4/3. ToxoDB #344 and #345 genotypes also had a new allele at the ROP5 locus. These results suggest high genetic diversity of T. gondii in southeastern México.

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墨西哥塔巴斯科市散养鸡刚地弓形虫分离株的新遗传变异和新的ROP5等位基因。

刚地弓形虫是一种原生动物寄生虫，可引起家禽和野生鸟类和哺乳动物（陆地和海洋）的感染。塔巴斯科州的气候和生态条件有利于这种寄生虫的繁殖和传播。因此，本研究的目的是采用PCR-RFLP法对15个遗传标记进行分离和分型。从7个市共获得12只鸡。采用改良凝集试验（MAT）对鸡血清进行血清学调查，12只鸡中有9只（75%）有弓形虫抗体（滴度≥1:10）。12只鸡中有8只组织中检测到弓形虫DNA。从8只鸡的心、脑标本中分离出12株有活力的弓形虫。采用10个多位点RFLP-PCR分型标记（SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）和5个毒力相关标记（CS3、ROP5、ROP16、ROP17和ROP18）对速殖子衍生DNA进行遗传鉴定。共鉴定出6种弓形虫PCR-RFLP基因型，包括#8（也称为BrIII型）、#28、#38和3种新的基因型，分别为#344、#345和#346。ROP18/ROP5等位基因组合分别为1/3、3/3和4/3。弓形虫344和345基因型在ROP5位点也有一个新的等位基因。这些结果表明，莫桑比克东南部弓形虫具有较高的遗传多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasitology 医学-寄生虫学

CiteScore

4.80

自引率

4.20%

发文量

280

审稿时长

3-8 weeks

期刊介绍： Parasitology is an important specialist journal covering the latest advances in the subject. It publishes original research and review papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in parasite biochemistry, molecular biology and genetics, ecology and epidemiology in the context of the biological, medical and veterinary sciences. Included in the subscription price are two special issues which contain reviews of current hot topics, one of which is the proceedings of the annual Symposia of the British Society for Parasitology, while the second, covering areas of significant topical interest, is commissioned by the editors and the editorial board.