Pflugers Archiv : European journal of physiology最新文献

Ethanol-induced dysfunction of the mesenteric perivascular adipose tissue is driven by mineralocorticoid receptors. 乙醇诱导的肠系膜血管周围脂肪组织功能障碍是由矿化皮质激素受体驱动的。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-16 DOI: 10.1007/s00424-025-03094-4

Ivis V O Martins, Thales M H Dourado, Gustavo F Pimenta, Marcela M Blascke de Mello, Aline G Fedoce, Wanessa M C Awata, Michele M Castro, Rita C Tostes, Carlos R Tirapelli

{"title":"Ethanol-induced dysfunction of the mesenteric perivascular adipose tissue is driven by mineralocorticoid receptors.","authors":"Ivis V O Martins, Thales M H Dourado, Gustavo F Pimenta, Marcela M Blascke de Mello, Aline G Fedoce, Wanessa M C Awata, Michele M Castro, Rita C Tostes, Carlos R Tirapelli","doi":"10.1007/s00424-025-03094-4","DOIUrl":"https://doi.org/10.1007/s00424-025-03094-4","url":null,"abstract":"The renin-angiotensin-aldosterone system (RAAS) is critical in ethanol-induced vascular dysfunction. Mineralocorticoid receptors (MR) trigger ethanol-induced vascular hypercontractility through pro-oxidative and pro-inflammatory effects. However, the contribution of MR to ethanol-induced perivascular adipose tissue (PVAT) dysfunction is unknown. Appreciating the importance of MR to PVAT dysfunction in distinctive pathological conditions, we investigated whether MR would play a role in ethanol-induced PVAT dysfunction. With this purpose, male Wistar Hannover rats were treated with ethanol 20% (in volume ratio) and/or potassium canrenoate [a MR antagonist (MRA); 30 mg/kg/day, gavage] for 5 weeks. Ethanol increased the circulating levels of aldosterone and impaired acetylcholine-induced relaxation of mesenteric arteries with, but not without PVAT. Antagonism of MR prevented ethanol-induced impairment in acetylcholine relaxation as well as the reduction of leptin levels and reactive oxygen species (ROS) overproduction in the mesenteric PVAT (mPVAT) from ethanol-treated rats. Ethanol promoted neutrophil accumulation and augmented the concentration of tumor necrosis factor (TNF)-α in the mPVAT and these responses were prevented by the MRA. Functional assays showed that tiron [a scavenger of superoxide (O2•-)] and etanercept (an antibody anti-TNF-α) failed to reverse the impairment of acetylcholine-induced relaxation promoted by ethanol. In mesenteric arteries, antagonism of MR prevented ROS generation, lipoperoxidation, and increased TNF-α levels induced by ethanol. In conclusion, our findings suggest that MR is involved in ethanol-induced dysfunction of mPVAT. This study enhances our understanding of how ethanol exerts harmful effects on the cardiovascular system, highlighting PVAT as a target for these detrimental effects.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermosensitivity of TREK K2P channels is controlled by a PKA switch and depends on the microtubular network. TREK K2P通道的热敏性由PKA开关控制，并取决于微管网络。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-15 DOI: 10.1007/s00424-025-03089-1

Sönke Cordeiro, Marianne Musinszki

{"title":"Thermosensitivity of TREK K2P channels is controlled by a PKA switch and depends on the microtubular network.","authors":"Sönke Cordeiro, Marianne Musinszki","doi":"10.1007/s00424-025-03089-1","DOIUrl":"https://doi.org/10.1007/s00424-025-03089-1","url":null,"abstract":"Temperature sensing is an essential component of animal perception and enables individuals to avoid painful or lethal temperatures. Many temperature sensors in central and peripheral neurons are ion channels. Here, we focus on the thermosensitive TREK/TRAAK subfamily of K2P channels-the only known K+ selective thermosensitive channels. The C-terminal domain is essential for the temperature activation of TREK channels, but the mechanism of temperature sensation and the nature of the temperature sensor are unknown. We studied the thermosensitivity of representatives of all K2P channel subfamilies and identified TREK-1 and TREK-2 as the only thermosensitive K2P channels, while TRAAK, the third member of the mechano-gated subfamily, showed no temperature dependence. We transferred the thermosensitivity of TREK-1 to TRAAK channels by exchanging the C-termini, demonstrating that the C-terminal domain is sufficient to confer thermosensitivity. By gradually truncating the C-terminus, we isolated a specific temperature responsive element (TRE) consisting of 18 amino acids that constitutes a unique feature in mammalian thermosensitive channels. Within this TRE lie both the binding domain for microtubule associated protein 2 (MAP2) and the PKA phosphorylation site. Pharmacological disruption of the microtubular network as well as the loss of the MAP2 binding site suppressed the temperature response, and PKA activation completely abolished temperature sensitivity. Thus, the connection to the microtubular network enables the thermosensitivity of TREK channels, which is not intrinsic to the channel itself, while the PKA-mediated phosphorylation status acts as a switch that determines if TREK channels are thermosensitive at all.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb. 小鼠肾厚升肢ROMK细胞异质性的表征。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-13 DOI: 10.1007/s00424-025-03086-4

Christian Keller, Rui Ramos Santos, Wouter H van Megen, Johannes Loffing

{"title":"Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb.","authors":"Christian Keller, Rui Ramos Santos, Wouter H van Megen, Johannes Loffing","doi":"10.1007/s00424-025-03086-4","DOIUrl":"https://doi.org/10.1007/s00424-025-03086-4","url":null,"abstract":"The renal thick ascending limb (TAL) plays a key role in water and ion homeostasis. Apical potassium secretion via the renal outer medullary potassium channel (ROMK) is essential for transepithelial sodium reabsorption via the furosemide-sensitive Na-K-2Cl-cotransporter and creates the electrochemical gradient for paracellular ion transport through Claudin tight junction proteins. Interestingly, the TAL exhibits transcriptomic heterogeneity and variable apical ROMK abundance. Single-cell RNA sequencing suggests that the cortical TAL consists of at least three distinct cell types, but whether ROMK distribution aligns with these types remains unclear. We analyzed perfusion-fixed mouse kidneys using RNAscope in situ hybridization (ISH), iterative indirect immunofluorescence imaging (4i multiplexing), and machine learning. ROMK mRNA expression was seen in all TAL cells. In contrast, apical ROMK protein abundance was found on almost all macula densa (MD) cells but was heterogeneous along the rest of the TAL. In the remaining TAL, only about 60% of the TAL cells had strong apical ROMK staining, while 40% lacked apical ROMK but showed weak perinuclear signals. ISH revealed that apical ROMK-positive cells express Ptger3 mRNA, whereas apical ROMK-negative cells express Foxq1 mRNA. Multiplexing analysis showed that ROMK-positive cells form Claudin-10b-positive tight junctions, while ROMK-negative cells form Claudin-16/19-positive junctions and express basolateral Kir4.1. Despite universal ROMK mRNA expression, apical ROMK distribution aligns with molecularly distinct TAL cell types. This unique ROMK expression pattern suggests functional heterogeneity for ROMK along the TAL.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated patch-clamp recordings for detecting activators and inhibitors of the epithelial sodium channel (ENaC). 用于检测上皮钠通道（ENaC）的激活剂和抑制剂的自动膜片钳记录。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-08 DOI: 10.1007/s00424-025-03087-3

Florian Sure, Markus Rapedius, Alexei Diakov, Marko Bertog, Alison Obergrussberger, Niels Fertig, Christoph Korbmacher, Alexandr V Ilyaskin

{"title":"Automated patch-clamp recordings for detecting activators and inhibitors of the epithelial sodium channel (ENaC).","authors":"Florian Sure, Markus Rapedius, Alexei Diakov, Marko Bertog, Alison Obergrussberger, Niels Fertig, Christoph Korbmacher, Alexandr V Ilyaskin","doi":"10.1007/s00424-025-03087-3","DOIUrl":"https://doi.org/10.1007/s00424-025-03087-3","url":null,"abstract":"The epithelial sodium channel (ENaC) is crucial for sodium absorption in several epithelial tissues including lung and kidney. Its involvement in various renal and pulmonary disorders makes ENaC a potential drug target. High-throughput screening using the automated patch-clamp (APC) technique appears to be a promising approach to discover novel ENaC modulators with (patho-)physiological and therapeutic implications. The aim of this methodological study was to establish APC measurements of ENaC-mediated currents. First, we confirmed functional expression of ENaC in a HEK293 cell line stably transfected with human αβγ-ENaC using conventional manual whole-cell patch-clamp recordings. For APC measurements, a standard enzymatic cell-detachment procedure was used to prepare single cell suspensions. This resulted in a high success rate of APC recordings with amiloride inhibitable ENaC currents. Using a γ-inhibitory peptide and the small molecule ENaC activator S3969, we demonstrate that APC recordings could reveal inhibitory as well as stimulatory effects on ENaC. Interestingly, the enzymatic cell-detachment protocol resulted in partial proteolytic ENaC activation. The portion of proteolytically activated channels could be reduced by prolonged incubation of suspended cells in cell culture medium. This recovery protocol enhanced the relative stimulatory effect of chymotrypsin, a prototypical serine protease known to cause proteolytic ENaC activation. Thus, this protocol may be particularly useful for identifying novel ENaC activators mimicking proteolytic channel activation. In conclusion, we have established a high-throughput screening method for the identification of novel ENaC activators and inhibitors using APC.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consecutive SSCs increase the SSC effect in skinned rat muscle fibres. 连续的SSC增加了皮肤大鼠肌纤维的SSC效应。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-08 DOI: 10.1007/s00424-025-03088-2

Tobias Elst, Sven Weidner, André Tomalka, Daniel Hahn, Florian Kurt Paternoster, Wolfgang Seiberl, Tobias Siebert

{"title":"Consecutive SSCs increase the SSC effect in skinned rat muscle fibres.","authors":"Tobias Elst, Sven Weidner, André Tomalka, Daniel Hahn, Florian Kurt Paternoster, Wolfgang Seiberl, Tobias Siebert","doi":"10.1007/s00424-025-03088-2","DOIUrl":"https://doi.org/10.1007/s00424-025-03088-2","url":null,"abstract":"Muscle function is essential for generating force and movement, with stretch-shortening cycles (SSCs) playing a fundamental role in the economy of everyday locomotion. Compared with pure shortening contractions, the SSC effect is characterised by increased force, work, and power output during the SSC shortening phase. Few studies have investigated whether SSC effects transfer across consecutive SSCs. Therefore, we investigated SSC effects over three consecutive SSCs in skinned rat muscle fibres by analysing the isometric force immediately before stretch onset (Fonset), the peak force at the end of stretching (Fpeak), and the minimum force at the end of shortening (Fmin), along with mechanical (WorkSSC) and shortening work (WorkSHO) at different activation levels (20%, 60%, and 100%). Each SSC was followed by an isometric hold phase, allowing force to return to a steady state. Results indicated an increase in both Fpeak (20.3%) and WorkSSC (60.9%) from SSC1 to SSC3 across all activation levels tested. At 20% and 60% activation, Fonset, Fmin, and WorkSHO increased (range: 4.5-28.5%) from SSC1 to SSC3. However, at 100% activation, Fonset and WorkSHO remained unchanged, while Fmin decreased (- 8.5%) from SSC1 to SSC3. These results suggest that the increase in SSC effects at submaximal activation may be primarily due to increased cross-bridge forces. The absence of increases in Fonset, Fmin, and WorkSHO at 100% activation suggests that increases in Fpeak and WorkSSC may not be attributed to increased cross-bridge force but could instead be caused by additional effects, possibly involving modulation of non-cross-bridge structures, likely titin, and their stiffness.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota, mitochondria, and epigenetics in health and disease: converging pathways to solve the puzzle. 健康和疾病中的微生物群、线粒体和表观遗传学：解决难题的趋同途径。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-01 Epub Date: 2025-03-20 DOI: 10.1007/s00424-025-03072-w

Natalia Lucia Rukavina Mikusic, Paula Denise Prince, Marcelo Roberto Choi, Luiz Gustavo A Chuffa, Vinícius Augusto Simão, Claudia Castro, Walter Manucha, Isabel Quesada

{"title":"Microbiota, mitochondria, and epigenetics in health and disease: converging pathways to solve the puzzle.","authors":"Natalia Lucia Rukavina Mikusic, Paula Denise Prince, Marcelo Roberto Choi, Luiz Gustavo A Chuffa, Vinícius Augusto Simão, Claudia Castro, Walter Manucha, Isabel Quesada","doi":"10.1007/s00424-025-03072-w","DOIUrl":"10.1007/s00424-025-03072-w","url":null,"abstract":"Dysbiosis, which refers to an imbalance in the composition of the gut microbiome, has been associated with a range of metabolic disorders, including type 2 diabetes, obesity, and metabolic syndrome. Although the exact mechanisms connecting gut dysbiosis to these conditions are not fully understood, various lines of evidence strongly suggest a substantial role for the interaction between the gut microbiome, mitochondria, and epigenetics. Current studies suggest that the gut microbiome has the potential to affect mitochondrial function and biogenesis through the production of metabolites. A well-balanced microbiota plays a pivotal role in supporting normal mitochondrial and cellular functions by providing metabolites that are essential for mitochondrial bioenergetics and signaling pathways. Conversely, in the context of illnesses, an unbalanced microbiota can impact mitochondrial function, leading to increased aerobic glycolysis, reduced oxidative phosphorylation and fatty acid oxidation, alterations in mitochondrial membrane permeability, and heightened resistance to cellular apoptosis. Mitochondrial activity can also influence the composition and function of the gut microbiota. Because of the intricate interplay between nuclear and mitochondrial communication, the nuclear epigenome can regulate mitochondrial function, and conversely, mitochondria can produce metabolic signals that initiate epigenetic changes within the nucleus. Given the epigenetic modifications triggered by metabolic signals from mitochondria in response to stress or damage, targeting an imbalanced microbiota through interventions could offer a promising strategy to alleviate the epigenetic alterations arising from disrupted mitochondrial signaling.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"635-655"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The NFAT5 chronicles: a transcription factor's tale of hypoxia, pulmonary drama, and endothelial resilience to hypoxia. NFAT5编年史：一个转录因子关于缺氧、肺戏剧和内皮对缺氧的恢复能力的故事。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-01 Epub Date: 2025-03-28 DOI: 10.1007/s00424-025-03080-w

Dörthe M Katschinski

引用次数: 0

Spatio-temporal segregation between sensory relay and swallowing pre-motor population activities by optical imaging in the rat nucleus of the solitary tract. 大鼠孤立束核感觉传递与吞咽前运动群体活动的时空分离。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-01 Epub Date: 2025-01-25 DOI: 10.1007/s00424-025-03065-9

Shinya Fuse, Yoichiro Sugiyama, Rishi R Dhingra, Shigeru Hirano, Mathias Dutschmann, Yasumasa Okada, Yoshitaka Oku

{"title":"Spatio-temporal segregation between sensory relay and swallowing pre-motor population activities by optical imaging in the rat nucleus of the solitary tract.","authors":"Shinya Fuse, Yoichiro Sugiyama, Rishi R Dhingra, Shigeru Hirano, Mathias Dutschmann, Yasumasa Okada, Yoshitaka Oku","doi":"10.1007/s00424-025-03065-9","DOIUrl":"10.1007/s00424-025-03065-9","url":null,"abstract":"The nucleus tractus solitarius (NTS) contains neurons that relay sensory swallowing commands information from the oropharyngeal cavity and swallowing premotor neurons of the dorsal swallowing group (DSG). However, the spatio-temporal dynamics of the interplay between the sensory relay and the DSG is not well understood. Here, we employed fluorescence imaging after microinjection of the calcium indicator into the NTS in an arterially perfused brainstem preparation of rat (n = 8) to investigate neuronal population activity in the NTS in response to superior laryngeal nerve (SLN) stimulation. Respiratory and swallowing motor activities were determined by simultaneous recordings of phrenic and vagal nerve activity (PNA, VNA). The analysis of SLN stimulation near the threshold triggering a swallowing allowed us to analyze Ca2+ signals related to the sensory relay and the DSG. We show that activation of sensory relay neurons triggers spatially confined Ca2+ signals exclusively unilateral to the stimulated SLN at short latencies (114.3 ± 94.4 ms). However, SLN-evoked swallowing triggered Ca2+ signals bilaterally at longer latencies (200 ± 145.2 ms) and engaged anatomically distributed DSG activity across the dorsal medulla oblongata. The Ca2+ signals originating from the DSG preceded evoked VNA swallow motor bursts, thus the swallowing premotor neurons that drive laryngeal motor pools are located outside the DSG. In conclusion, the study illuminates the spatial-temporal features of sensory-motor integration of swallowing in the NTS and further supports the hypothesis that the NTS harbors swallowing pre-motor neurons that may generate the swallowing motor activity, while first-order pre-motor pools are located outside the DSG.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"719-727"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology of metabolic syndrome. 代谢综合征的流行病学。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-01 Epub Date: 2025-01-25 DOI: 10.1007/s00424-024-03051-7

Iris Pigeot, Wolfgang Ahrens

{"title":"Epidemiology of metabolic syndrome.","authors":"Iris Pigeot, Wolfgang Ahrens","doi":"10.1007/s00424-024-03051-7","DOIUrl":"10.1007/s00424-024-03051-7","url":null,"abstract":"The global increase of overweight and obesity in children and adults is one of the most prominent public health threats, often accompanied by insulin resistance, hypertension, and dyslipidemia. The simultaneous occurrence of these health problems is referred to as metabolic syndrome. Various criteria have been proposed to define this syndrome, but no general consensus on the specific markers and the respective cut-offs has been achieved yet. As a consequence, it is difficult to assess regional variations and temporal trends and to obtain a comprehensive picture of the global burden of this major health threat. This limitation is most striking in childhood and adolescence, when metabolic parameters change with developmental stage. Obesity and related metabolic disorders develop early in life and then track into adulthood, i.e., the metabolic syndrome seems to originate in the early life course. Thus, it would be important to monitor the trajectories of cardio-metabolic parameters from early on. We will summarize selected key studies to provide a narrative overview of the global epidemiology of the metabolic syndrome while considering the limitations that hinder us to provide a comprehensive full picture of the problem. A particular focus will be given to the situation in children and adolescents and the risk factors impacting on their cardio-metabolic health. This summary will be complemented by key findings of a pan-European children cohort and first results of a large German adult cohort.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"669-680"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The enigma of ENaC activation by proteolytic cleavage: a never ending quest? ENaC蛋白水解裂解激活之谜：永无止境的探索？

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2025-05-01 Epub Date: 2025-03-31 DOI: 10.1007/s00424-025-03081-9

Eric Feraille, Ali Sassi, Monika Gjorgjieva

引用次数: 0