Pflugers Archiv : European journal of physiology最新文献

Stability of N-type inactivation and the coupling between N-type and C-type inactivation in the Aplysia Kv1 channel. plysia Kv1 通道中 N 型失活的稳定性以及 N 型和 C 型失活之间的耦合。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-15 DOI: 10.1007/s00424-024-02982-5

Tokunari Iwamuro, Kazuki Itohara, Yasuo Furukawa

{"title":"Stability of N-type inactivation and the coupling between N-type and C-type inactivation in the Aplysia Kv1 channel.","authors":"Tokunari Iwamuro, Kazuki Itohara, Yasuo Furukawa","doi":"10.1007/s00424-024-02982-5","DOIUrl":"10.1007/s00424-024-02982-5","url":null,"abstract":"The voltage-dependent potassium channels (Kv channels) show several different types of inactivation. N-type inactivation is a fast inactivating mechanism, which is essentially an open pore blockade by the amino-terminal structure of the channel itself or the auxiliary subunit. There are several functionally discriminatable slow inactivation (C-type, P-type, U-type), the mechanism of which is supposed to include rearrangement of the pore region. In some Kv1 channels, the actual inactivation is brought about by coupling of N-type and C-type inactivation (N-C coupling). In the present study, we focused on the N-C coupling of the Aplysia Kv1 channel (AKv1). AKv1 shows a robust N-type inactivation, but its recovery is almost thoroughly from C-type inactivated state owing to the efficient N-C coupling. In the I8Q mutant of AKv1, we found that the inactivation as well as its recovery showed two kinetic components apparently correspond to N-type and C-type inactivation. Also, the cumulative inactivation which depends on N-type mechanism in AKv1 was hindered in I8Q, suggesting that N-type inactivation of I8Q is less stable. We also found that Zn <math><msup><mrow></mrow> <mrow><mn>2</mn> <mo>+</mo></mrow> </msup> </math> specifically accelerates C-type inactivation of AKv1 and that H382 in the pore turret is involved in the Zn <math><msup><mrow></mrow> <mrow><mn>2</mn> <mo>+</mo></mrow> </msup> </math> binding. Because the region around Ile <math><msup><mrow></mrow> <mn>8</mn></msup> </math> (I8) in AKv1 has been suggested to be involved in the pre-block binding of the amino-terminal structure, our results strengthen a hypothesis that the stability of the pre-block state is important for stable N-type inactivation as well as the N-C coupling in the Kv1 channel inactivation.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mild hyperbaric oxygen exposure protects heart during ischemia/reperfusion and affects vascular relaxation. 轻度高压氧暴露可在缺血/再灌注期间保护心脏，并影响血管松弛。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-25 DOI: 10.1007/s00424-024-02992-3

Christopher Gutierrez, Magdalena Peirone, Andrea Carranza, Guillermo Di Girolamo, Patricia Bonazzola, Rocío Castilla

{"title":"Mild hyperbaric oxygen exposure protects heart during ischemia/reperfusion and affects vascular relaxation.","authors":"Christopher Gutierrez, Magdalena Peirone, Andrea Carranza, Guillermo Di Girolamo, Patricia Bonazzola, Rocío Castilla","doi":"10.1007/s00424-024-02992-3","DOIUrl":"10.1007/s00424-024-02992-3","url":null,"abstract":"Mild hyperbaric oxygen therapy (mHBOT) is an adjuvant therapy used in conditions where tissue oxygenation is reduced and is implemented using pressures less than 1.5 ATA and 100% O2 (instead of the classical HBOT at 1.9-3 ATA) which results in cheaper, easier to implement, and equally effective. mHBOT is offered for wellness and beauty and as an anti-aging strategy, in spite of the absence of studies on the cardiovascular system. Consequently, we investigated the impact of mHBOT on the cardiovascular system. Mechanical and energetic parameters of isolated heart submitted to ischemia/reperfusion injury and arterial contractile response from mHBOT-exposed rats were evaluated. In the heart, mHBOT increased pre-ischemic velocity of contraction and ischemic end-diastolic pressure and developed pressure and contractile economy during reperfusion. mHBOT decreased infarct size and increased the plasma nitrite levels. In the artery, mHBOT increased acetylcholine sensitivity. mHBOT protects the heart during ischemia/reperfusion and affects vascular relaxation.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of hypoxia on GLP-1 secretion - an in vitro study using enteroendocrine STC-1 -cells as a model. 缺氧对 GLP-1 分泌的影响--以肠内分泌 STC-1 细胞为模型的体外研究。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-29 DOI: 10.1007/s00424-024-02996-z

Ravikant Sharma, Ghulam Shere Raza, Nalini Sodum, Jaroslaw Walkowiak, Karl-Heinz Herzig

{"title":"Effect of hypoxia on GLP-1 secretion - an in vitro study using enteroendocrine STC-1 -cells as a model.","authors":"Ravikant Sharma, Ghulam Shere Raza, Nalini Sodum, Jaroslaw Walkowiak, Karl-Heinz Herzig","doi":"10.1007/s00424-024-02996-z","DOIUrl":"10.1007/s00424-024-02996-z","url":null,"abstract":"Glucagon-like peptide (GLP)-1 is a hormone released by enteroendocrine L-cells after food ingestion. L-cells express various receptors for nutrient sensing including G protein-coupled receptors (GPRs). Intestinal epithelial cells near the lumen have a lower O2 tension than at the base of the crypts, which leads to hypoxia in L-cells. We hypothesized that hypoxia affects nutrient-stimulated GLP-1 secretion from the enteroendocrine cell line STC-1, the most commonly used model. In this study, we investigated the effect of hypoxia (1% O2) on alpha-linolenic acid (αLA) stimulated GLP-1 secretion and their receptor expressions. STC-1 cells were incubated for 12 h under hypoxia (1% O2) and treated with αLA to stimulate GLP-1 secretion. 12 h of hypoxia did not change basal GLP-1 secretion, but significantly reduced nutrient (αLA) stimulated GLP-1 secretion. In normoxia, αLA (12.5 μM) significantly stimulated (~ 5 times) GLP-1 secretion compared to control, but under hypoxia, GLP-1 secretion was reduced by 45% compared to normoxia. αLA upregulated GPR120, also termed free fatty acid receptor 4 (FFAR4), expressions under normoxia as well as hypoxia. Hypoxia downregulated GPR120 and GPR40 expression by 50% and 60%, respectively, compared to normoxia. These findings demonstrate that hypoxia does not affect the basal GLP-1 secretion but decreases nutrient-stimulated GLP-1 secretion. The decrease in nutrient-stimulated GLP-1 secretion was due to decreased GPR120 and GPR40 receptors expression. Changes in the gut environment and inflammation might contribute to the hypoxia of the epithelial and L-cells.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of claudin-3 does not alter intestinal absorption of phosphate in mice. 缺少 claudin-3 不会改变小鼠肠道对磷酸盐的吸收。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1007/s00424-024-02998-x

Zsuzsa Radványi, Udo Schnitzbauer, Eva Maria Pastor-Arroyo, Simone Hölker, Nina Himmerkus, Markus Bleich, Dominik Müller, Tilman Breiderhoff, Nati Hernando, Carsten A Wagner

{"title":"Absence of claudin-3 does not alter intestinal absorption of phosphate in mice.","authors":"Zsuzsa Radványi, Udo Schnitzbauer, Eva Maria Pastor-Arroyo, Simone Hölker, Nina Himmerkus, Markus Bleich, Dominik Müller, Tilman Breiderhoff, Nati Hernando, Carsten A Wagner","doi":"10.1007/s00424-024-02998-x","DOIUrl":"10.1007/s00424-024-02998-x","url":null,"abstract":"Intestinal absorption of phosphate is bimodal, consisting of a transcellular pathway and a poorly characterized paracellular mode, even though the latter one contributes to the bulk of absorption under normal dietary conditions. Claudin-3 (Cldn3), a tight junction protein present along the whole intestine in mice, has been proposed to tighten the paracellular pathway for phosphate. The aim of this work was to characterize the phosphate-related phenotype of Cldn3-deficient mice. Cldn3-deficient mice and wildtype littermates were fed standard diet or challenged for 3 days with high dietary phosphate. Feces, urine, blood, intestinal segments and kidneys were collected. Measurements included fecal, urinary, and plasma concentrations of phosphate and calcium, plasma levels of phosphate-regulating hormones, evaluation of trans- and paracellular phosphate transport across jejunum and ileum, and analysis of intestinal phosphate and calcium permeabilities. Fecal and urinary excretion of phosphate as well as its plasma concentration was similar in both genotypes, under standard and high-phosphate diet. However, Cldn3-deficient mice challenged with high dietary phosphate had a reduced urinary calcium excretion and increased plasma levels of calcitriol. Intact FGF23 concentration was also similar in both groups, regardless of the dietary conditions. We found no differences either in intestinal phosphate transport (trans- or paracellular) and phosphate and calcium permeabilities between genotypes. The intestinal expression of claudin-7 remained unaltered in Cldn3-deficient mice. Our data do not provide evidence for a decisive role of Cldn3 for intestinal phosphate absorption and phosphate homeostasis. In addition, our data suggest a novel role of Cldn3 in regulating calcitriol levels.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging connections between Piezo1 and BK channels in vascular smooth muscle cells. 血管平滑肌细胞中 Piezo1 和 BK 通道之间的新联系

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-08-01 DOI: 10.1007/s00424-024-03001-3

Luigi Catacuzzeno, Antonio Michelucci

引用次数: 0

Impact of lengthening velocity on the generation of eccentric force by slow-twitch muscle fibers in long stretches. 拉伸速度对慢速肌纤维在长距离拉伸中产生偏心力的影响。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-24 DOI: 10.1007/s00424-024-02991-4

Sven Weidner, André Tomalka, Christian Rode, Tobias Siebert

{"title":"Impact of lengthening velocity on the generation of eccentric force by slow-twitch muscle fibers in long stretches.","authors":"Sven Weidner, André Tomalka, Christian Rode, Tobias Siebert","doi":"10.1007/s00424-024-02991-4","DOIUrl":"10.1007/s00424-024-02991-4","url":null,"abstract":"After an initial increase, isovelocity elongation of a muscle fiber can lead to diminishing (referred to as Give in the literature) and subsequently increasing force. How the stretch velocity affects this behavior in slow-twitch fibers remains largely unexplored. Here, we stretched fully activated individual rat soleus muscle fibers from 0.85 to 1.3 optimal fiber length at stretch velocities of 0.01, 0.1, and 1 maximum shortening velocity, vmax, and compared the results with those of rat EDL fast-twitch fibers obtained in similar experimental conditions. In soleus muscle fibers, Give was 7%, 18%, and 44% of maximum isometric force for 0.01, 0.1, and 1 vmax, respectively. As in EDL fibers, the force increased nearly linearly in the second half of the stretch, although the number of crossbridges decreased, and its slope increased with stretch velocity. Our findings are consistent with the concept of a forceful detachment and subsequent crossbridge reattachment in the stretch's first phase and a strong viscoelastic titin contribution to fiber force in the second phase of the stretch. Interestingly, we found interaction effects of stretch velocity and fiber type on force parameters in both stretch phases, hinting at fiber type-specific differences in crossbridge and titin contributions to eccentric force. Whether fiber type-specific combined XB and non-XB models can explain these effects or if they hint at some not fully understood properties of muscle contraction remains to be shown. These results may stimulate new optimization perspectives in sports training and provide a better understanding of structure-function relations of muscle proteins.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galectin-3 protects distal convoluted tubules in rhabdomyolysis-induced kidney injury. 在横纹肌溶解引起的肾损伤中，Galectin-3 可保护远端曲小管。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-23 DOI: 10.1007/s00424-024-02987-0

Vera A Kulow, Robert Labes, Claudia S Czopek, Christian Rosenberger, Michael Fähling

{"title":"Galectin-3 protects distal convoluted tubules in rhabdomyolysis-induced kidney injury.","authors":"Vera A Kulow, Robert Labes, Claudia S Czopek, Christian Rosenberger, Michael Fähling","doi":"10.1007/s00424-024-02987-0","DOIUrl":"10.1007/s00424-024-02987-0","url":null,"abstract":"Advanced glycation endproducts (AGEs) contribute to cellular damage of various pathologies, including kidney diseases. Acute kidney injury (AKI) represents a syndrome seldom characterized by a single, distinct pathophysiological cause. Rhabdomyolysis-induced acute kidney injury (RIAKI) constitutes roughly 15% of AKI cases, yet its underlying pathophysiology remains poorly understood. Using a murine model of RIAKI induced by muscular glycerol injection, we observed elevated levels of AGEs and the AGE receptor galectin-3 (LGALS3) in the kidney. Immunofluorescence localized LGALS3 to distal nephron segments. According to transcriptomic profiling via next-generation sequencing, RIAKI led to profound changes in kidney metabolism, oxidative stress, and inflammation. Cellular stress was evident in both proximal and distal tubules, as shown by kidney injury markers KIM-1 and NGAL. However, only proximal tubules exhibited overt damage and apoptosis, as detected by routine morphology, active Caspase-3, and TUNEL assay, respectively. In vitro, distal convoluted tubule (DCT) cells challenged with AGEs underwent apoptosis, which was markedly enhanced by Lgals3 siRNA treatment. Thus, in RIAKI, the upregulation of LGALS3 may protect the distal nephron from AGE-mediated damage, while proximal tubules lacking LGALS3 stay at risk. Thus, stimulating LGALS3 in the proximal nephron, if achievable, may attenuate RIAKI.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration of Piezo1 signaling in type 2 diabetic mice: focus on endothelium and BK_Ca channel. 2 型糖尿病小鼠体内 Piezo1 信号的改变：关注血管内皮和 BKCa 通道。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-02 DOI: 10.1007/s00424-024-02983-4

Chae Eun Haam, Sooyeon Choi, Seonhee Byeon, Eun Yi Oh, Soo-Kyoung Choi, Young-Ho Lee

{"title":"Alteration of Piezo1 signaling in type 2 diabetic mice: focus on endothelium and BKCa channel.","authors":"Chae Eun Haam, Sooyeon Choi, Seonhee Byeon, Eun Yi Oh, Soo-Kyoung Choi, Young-Ho Lee","doi":"10.1007/s00424-024-02983-4","DOIUrl":"10.1007/s00424-024-02983-4","url":null,"abstract":"Piezo1 mechanosensitive ion channel plays a important role in vascular physiology and disease. This study aimed to elucidate the altered signaling elicited by Piezo1 activation in the arteries of type 2 diabetes. Ten- to 12-week-old male C57BL/6 (control) and type 2 diabetic mice (db-/db-) were used. The second-order mesenteric arteries (~ 150 μm) were used for isometric tension experiments. Western blot analysis and immunofluorescence staining were performed to observe protein expression. Piezo1 was significantly decreased in mesenteric arteries of type 2 diabetic mice compared to control mice, as analyzed by western blot and immunofluorescence staining. Piezo1 agonist, Yoda1, concentration-dependently induced relaxation of mesenteric arteries in both groups. Interestingly, the relaxation response was significantly greater in control mice than in db-/db- mice. The removal of endothelium reduced relaxation responses induced by Yoda1, which was greater in control mice than db-/db- mice. Furthermore, the relaxation response was reduced by pre-treatment with various types of K+ channel blockers in endothelium-intact arteries in control mice. In endothelium-denuded arteries, pre-incubation with charybdotoxin, an Ca2+-activated K+ channel (BKCa channel) blocker, significantly attenuated Yoda1-induced relaxation in db-/db- mice, while there was no effect in control mice. Co-immunofluorescence staining showed co-localization of Piezo1 and BKCa channel was more pronounced in db-/db- mice than in control mice. These results indicate that the vascular responses induced by Piezo1 activation are different in the mesenteric resistance arteries in type 2 diabetic mice.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding influences of indoor temperature and light on neural activity: entropy analysis of electroencephalographic signals. 解码室内温度和光线对神经活动的影响：脑电信号的熵分析。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-16 DOI: 10.1007/s00424-024-02988-z

Chiara Pappalettera, Silvia Angela Mansi, Marco Arnesano, Fabrizio Vecchio

{"title":"Decoding influences of indoor temperature and light on neural activity: entropy analysis of electroencephalographic signals.","authors":"Chiara Pappalettera, Silvia Angela Mansi, Marco Arnesano, Fabrizio Vecchio","doi":"10.1007/s00424-024-02988-z","DOIUrl":"10.1007/s00424-024-02988-z","url":null,"abstract":"Understanding the neural responses to indoor characteristics like temperature and light is crucial for comprehending how the physical environment influences the human brain. Our study introduces an innovative approach using entropy analysis, specifically, approximate entropy (ApEn), applied to electroencephalographic (EEG) signals to investigate neural responses to temperature and light variations in indoor environments. By strategically placing electrodes over specific brain regions linked to temperature and light processing, we show how ApEn can be influenced by indoor factors. We also integrate heart indices from a multi-sensor bracelet to create a machine learning classifier for temperature conditions. Results showed that in anterior frontal and temporoparietal areas, neutral temperature conditions yield higher ApEn values. The anterior frontal area showed a trend of gradually decreasing ApEn values from neutral to warm conditions, with cold being in an intermediate position. There was a significant interaction between light and site factors, only evident in the temporoparietal region. Here, the neutral light condition had higher ApEn values compared to blue and red light conditions. Positive correlations between anterior frontal ApEn and thermal comfort scores suggest a link between entropy and perceived thermal comfort. Our quadratic SVM classifier, incorporating entropy and heart features, demonstrates strong performance (until 90% in terms of AUC, accuracy, sensitivity, and specificity) in classifying temperature sensations. This study offers insights into neural responses to indoor factors and presents a novel approach for temperature classification using EEG entropy and heart features.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the relationship between intercostal muscle oxygenation measured by near-infrared spectroscopy and exercise capacity in group E COPD patients. 评估通过近红外光谱测量的 E 组慢性阻塞性肺病患者肋间肌氧合与运动能力之间的关系。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2024-10-01 Epub Date: 2024-07-24 DOI: 10.1007/s00424-024-02993-2

Buğra Kerget, Gizem Çil, Alperen Aksakal

{"title":"Evaluation of the relationship between intercostal muscle oxygenation measured by near-infrared spectroscopy and exercise capacity in group E COPD patients.","authors":"Buğra Kerget, Gizem Çil, Alperen Aksakal","doi":"10.1007/s00424-024-02993-2","DOIUrl":"10.1007/s00424-024-02993-2","url":null,"abstract":"Near-infrared spectroscopy (NIRS) can be used to demonstrate muscle metabolism and oxygenation. NIRS-based oximeters enable the noninvasive measurement of static and dynamic muscle oxygenation. This study aimed to evaluate the relationship between NIRS readings and exercise capacity in group E COPD patients. The prospective study included 40 patients with group E COPD who presented to our outpatient clinic between May 2021 and June 2022. The patients were evaluated with pulmonary function testing, 6-Minute Walk Test (6MWT), echocardiography, and dyspnea and quality of life assessments. NIRS muscle oxygen saturation (SmO2) levels at the start and end of the 6MWT were obtained. 6MWT distance was positively correlated with intercostal SmO2 and fingertip SO2 at the start (R = 0.679, p ≤ 0.001 and R = 0.321, p = 0.04, respectively) and end of the 6MWT (R = 0.693, p ≤ 0.001 and R = 0.635, p ≤ 0.001, respectively) and negatively correlated with the number of hospitalizations due to exacerbations in the last year and mean pulmonary arterial pressure (R = - 0.648, p ≤ 0.001 and R = - 0.676, p ≤ 0.001, respectively). SF-36 score was positively correlated with intercostal SmO2 at the beginning of the 6MWT (R = 0.336, p = 0.03). Intercostal SmO2 levels at the start of the 6MWT positively correlated with diffusing capacity of the lung for carbon dioxide (DLCO) (R = 0.388, p = 0.01) and ratio of DLCO to alveolar volume (DLCO/VA) levels (R = 0.379, p = 0.02), and these correlations persisted more strongly after the 6MWT (R = 0.524, p = 0.01; R = 0.500, p = 0.01, respectively). NIRS is a practical and noninvasive method for measuring muscle oxygenation and can be used as an alternative to 6MWT in the evaluation of exercise capacity in patients with group E COPD.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0