Pflugers Archiv : European journal of physiology最新文献_第2页

Upregulation of T-type voltage-dependent Ca²⁺ channels in activated hepatic stellate cells promotes liver fibrosis in metabolic dysfunction-associated steatohepatitis. 激活肝星状细胞中t型电压依赖性Ca 2 +通道的上调促进代谢功能障碍相关脂肪性肝炎的肝纤维化。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-31 DOI: 10.1007/s00424-026-03164-1

Naoki Kawata, Rubii Kondo, Mizuki Hashizume, Yoshiaki Suzuki, Hisao Yamamura

引用次数: 0

Pathophysiology of homocysteine: insights into ion channel dysfunction. 同型半胱氨酸的病理生理学：离子通道功能障碍的见解。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-31 DOI: 10.1007/s00424-026-03165-0

Nikola Chmúrčiaková, Robin N Stringer, Leoš Cmarko, Alzbeta Filipova, Lubica Lacinova, Norbert Weiss

引用次数: 0

The effects of elevated phosphate on the kidney - damaging the gatekeeper. 升高的磷酸盐对肾脏的影响——损害守门人。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-30 DOI: 10.1007/s00424-026-03160-5

Tanecia Mitchell, Vivek Verma, Abul Fajol, Christian Faul

引用次数: 0

DNase I as a probe for unpolymerized actin: revisiting a classic tool for nuclear actin research. dna酶I作为未聚合肌动蛋白的探针：回顾核肌动蛋白研究的经典工具。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-26 DOI: 10.1007/s00424-026-03158-z

Anika Göpel, Laura Bauer, Dörthe M Katschinski, Anke Zieseniss

引用次数: 0

Targeting hypertension through natural phenolics: The multifaceted role of cinnamic acid and its derivatives. 通过天然酚类物质治疗高血压：肉桂酸及其衍生物的多方面作用。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-26 DOI: 10.1007/s00424-026-03163-2

Samuel S P de Araujo, Brenda L B Dos Santos, Waldilleny R A de Moura, Leonardo G Rodrigues, Luiz G S Branco, Helio C Salgado, Renato N Soriano, Marina de T Durand, João Paulo J Sabino

引用次数: 0

Nitrergic neurotransmission within the lateral hypothalamus modulates cardiovascular responses to stress in rats. 外侧下丘脑内的氮能神经传递调节大鼠对应激的心血管反应。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-24 DOI: 10.1007/s00424-026-03161-4

Gabriela A Silva, Lilian L Reis-Silva, Lucas Gomes-de-Souza, Lígia R R Tavares, Lucas Barretto-de-Souza, Vinícius Pelarin, Carlos C Crestani

{"title":"Nitrergic neurotransmission within the lateral hypothalamus modulates cardiovascular responses to stress in rats.","authors":"Gabriela A Silva, Lilian L Reis-Silva, Lucas Gomes-de-Souza, Lígia R R Tavares, Lucas Barretto-de-Souza, Vinícius Pelarin, Carlos C Crestani","doi":"10.1007/s00424-026-03161-4","DOIUrl":"10.1007/s00424-026-03161-4","url":null,"abstract":"The lateral hypothalamus (LH) is a key brain region involved in integrating cardiovascular and autonomic components of the stress response, yet the local neurochemical mechanisms governing these functions remain poorly understood. This study examined the contribution of nitrergic neurotransmission within the LH to cardiovascular adjustments induced by acute restraint stress in rats. To this end, animals received bilateral microinjections into the LH of either the nonselective nitric oxide synthase (NOS) inhibitor L-NAME, the selective neuronal NOS (nNOS) inhibitor Nω-propyl-L-arginine hydrochloride (NPLA), the selective inducible NOS (iNOS) inhibitor 1400W, or the nitric oxide (NO) donor MAHMA NONOate (NOC-9) prior to the restraint session. Intra-LH administration of L-NAME significantly attenuated both the pressor and tachycardic responses elicited by restraint. In contrast, the selective nNOS inhibitor NPLA selectively enhanced the tachycardic response without altering the pressor effect or sympathetically-mediated cutaneous vasoconstriction. Consistent with the effect observed in NPLA-treated animals, facilitation of local nitrergic neurotransmission through intra-LH injection of the NO donor selectively reduced the heart rate response to restraint. The selective iNOS inhibitor 1400W did not affect any of the cardiovascular parameters measured. Taken together, these findings indicate that nitrergic signaling within the LH plays a critical role in modulating cardiovascular adjustments to acute stress. This regulation appears to involve a complex process involving nNOS and an additional nitrergic mechanism that acts independently of iNOS.","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":"478 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13013230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low fruit and vegetable intake in children: a dietary stressor raising renal ammonium and adrenal cortisol secretion. 儿童水果和蔬菜摄入量低：一种提高肾铵和肾上腺皮质醇分泌的饮食应激源。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-23 DOI: 10.1007/s00424-026-03162-3

Thomas Remer, Yifan Hua, Jonas Esche, Lijie Shi, Michaela F Hartmann, Stefan A Wudy

引用次数: 0

PKC isoform-specific targeting of the phosphorylation site Serine¹⁹¹ in the GIRK4 subunit induces receptor-dependent modulation of GIRK channel activity. PKC亚型特异性靶向磷酸化位点丝氨酸191在GIRK4亚基诱导受体依赖性调节GIRK通道活性。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-19 DOI: 10.1007/s00424-026-03157-0

Leonie Inderwiedenstraße, Marie-Cécile Kienitz

引用次数: 0

CO and N₂ are not humane alternatives to CO₂ euthanasia in rats. 一氧化碳和氮气不是对老鼠进行二氧化碳安乐死的人道选择。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-14 DOI: 10.1007/s00424-026-03156-1

Charlotte Calvet, Philipp Villiger, Francesco Prisco, Tim Buchholz, Felix Gantenbein, Christa Thöne-Reineke, Boris Gourévitch, Carsten A Wagner, Petra Seebeck

引用次数: 0

Structural determinants of voltage sensitivity in hyperpolarization-activated ion channels and their persistence in the evolutionary scale. 超极化激活离子通道中电压敏感性的结构决定因素及其在进化尺度上的持久性。

IF 2.9 4区医学

Pflugers Archiv : European journal of physiology Pub Date : 2026-03-07 DOI: 10.1007/s00424-026-03153-4

Karla G Alvarez-Villagómez, Daniel Balleza

{"title":"Structural determinants of voltage sensitivity in hyperpolarization-activated ion channels and their persistence in the evolutionary scale.","authors":"Karla G Alvarez-Villagómez, Daniel Balleza","doi":"10.1007/s00424-026-03153-4","DOIUrl":"10.1007/s00424-026-03153-4","url":null,"abstract":"HCN channels have a reverse electromechanical coupling mechanism, where hyperpolarized membrane potentials facilitate pore opening through an inward displacement of the S4 segment of the voltage-sensing domain (VSD). This voltage dependence is finely regulated by the binding of cAMP to an intracellular domain (CNBD). Of the four widely studied isoforms of human HCN channels, the HCN3 channel is practically insensitive to cAMP or is even inhibited by it, but the structural determinants underlying this unexpected behavior are still unclear. Here, we evaluated the possible role of flexibility in very specific regions of the VSD that could be determinants for this behavior. Part of the S2-S3L linker is more rigid in HCN3, which correlates with low atomic mobility for this region in proximity to the C-linker subdomain of the opposite subunit. We built structural models using AlphaFold 3 and Swiss-Model and thus reconstructed the disordered regions that connect the transmembrane segments of the VSD and that in some of the structures deposited in the PDB have not been resolved. Besides, in an attempt to reveal the evolutionary trends that this transmembrane domain may have undergone, we conducted a comparative study with phylogenetically distant HCN channels and found an interesting tendency to lose sensitivity to cAMP as VSD flexibility is lost. Our analysis confirms a large body of published experimental findings. Finally, we found that in metazoans, two types of HCN channels clearly diverge: (1) those that are highly sensitive to cAMP with moderate flexibility profiles in protostomes, and (2) those that show less marked sensitivity to this ligand in deuterostomes. We also propose a possible evolutionary scenario for the appearance of cAMP-modulated HCN channels in the last eukaryotic common ancestor (LECA).","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":"478 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0