Pflugers Archiv : European journal of physiology最新文献

{"title":"Resistance exercise training attenuates skeletal muscle atrophy in experimental pulmonary arterial hypertension.","authors":"Sebastião Felipe Ferreira Costa, Luciano Bernardes Leite, Leôncio Lopes Soares, Sara Caco Dos Lúcio Generoso, Mirielly Alexia Miranda Xavier, Matheus Soares Faria, Arthur Eduardo de Carvalho Quintão, Luiz Otávio Guimarães Ervilha, Thainá Iasbik Lima, Bruno Rocha Avila Pelozin, Tiago Fernandes, Edilamar Menezes Oliveira, Mariana Machado Neves, Emily Correna Carlo Reis, Leandro Licursi Oliveira, Antônio José Natali","doi":"10.1007/s00424-025-03142-z","DOIUrl":"10.1007/s00424-025-03142-z","url":null,"abstract":"<p><p>To evaluate the effects of a resistance training (RT) program applied during the development of MCT-induced pulmonary arterial hypertension (PAH) on skeletal muscle atrophy in rats. Twenty-one male Wistar rats were randomly distributed into three experimental groups (n = 7 per group): Sedentary Control (SC), Sedentary Hypertensive (SH), and Trained Hypertensive (TH). PAH was induced by a single intraperitoneal injection of monocrotaline (MCT; 60 mg/kg). Animals in the TH group underwent RT (vertical ladder; 15 climbs with 1-minute interval; 60% of the maximum load supported), 1 session/day, 5 days/week, for approximately 3 weeks. On the 24th day after injection, all animals were euthanized. Subsequently, the biceps brachii were removed, processed and destined for histological or biochemical analyses. RT increased the exercise tolerance (i.e., maximum load supported) in rats with PAH. In addition, RT prevented adverse remodeling in skeletal muscle by preserving the cross-sectional area of myocytes and attenuated total collagen deposition. Furthermore, RT reduced the gene expression of proteolytic agents (i.e., MuRF1, atrogin-1, and myostatin) and attenuated redox imbalance (i.e., CAT, NO, and CP). However, neither PAH nor RT influenced muscle hypertrophy pathways (i.e., Akt, phospo-Akt, eIF4E e phospo- eIF4E) in this model. The RT applied during the development of MCT-induced PAH protects against skeletal muscle atrophy, by mitigating adverse structural remodeling and atrophy through proteolysis modulation and attenuation of redox imbalance.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":"478 1","pages":"3"},"PeriodicalIF":3.2,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic syndrome reduces but does not eliminate the cardioprotective effect of adaptation to hypoxia: the link with changes in the opioid system.","authors":"Natalia V Naryzhnaya, Ivan A Derkachev, Boris K Kurbatov, Alexander V Mukhomedzyanov, Mikhail Kilin, Artur Kan, Alexandr E Grab, Alla A Boschenko, Leonid N Maslov","doi":"10.1007/s00424-025-03144-x","DOIUrl":"10.1007/s00424-025-03144-x","url":null,"abstract":"<p><p>Chronic continuous hypoxia (CCH) demonstrates a pronounced protective effect in cardiac ischemia-reperfusion (IR). Opioids and opioid receptors play a significant role in this process. It has been previously shown that metabolic syndrome (MS) impairs the development of adaptive myocardial resistance to IR. The aim of this study is to identify the relationship between the cardioprotective effect of CCH and opioid peptides in circulating blood and myocardial tissue, as well as the expression of opioid receptors in the myocardium of rats with and without MS. All rats were subjected to coronary artery occlusion (45 min) and reperfusion (2 h). Some rats received high carbohydrate high fat diet for 90 days (MS) before IR. Some animals were exposed to CCH (21 days, 12% O₂) before IR. The plasma endomorphin-2, and dynorphin A levels were increased after CCH. Endomorphin-2, β-endorphin, dynorphin A (1-13), and met-enkephalin content in myocardial tissue was increased in response to CCH. In rats with MS, an increase in the plasma and myocardial opioid levels in response to CCH was reduced. A correlation was identified between plasma and myocardial tissue opioid peptide levels and the extent of myocardial injury, as well as between myocardial opioid peptide content and contractility. Furthermore, CCH and MS caused a reduction in the expression of δ- (DOR) and κ- (KOR) opioid receptors, and also an increase in µ- (MOR) opioid receptor expression. These findings suggest that a decrease in opioid peptide content could impair adaptive cardioprotection in MS.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":"478 1","pages":"2"},"PeriodicalIF":3.2,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of low-dose lithium treatment on rat postural muscle under conditions of disuse.","authors":"Timur M Mirzoev, Sergey A Tyganov, Olga V Turtikova, Vitaly E Kalashnikov, Kristina A Sharlo, Boris S Shenkman","doi":"10.1007/s00424-025-03138-9","DOIUrl":"10.1007/s00424-025-03138-9","url":null,"abstract":"<p><p>Skeletal muscles undergo significant atrophy and loss of strength under conditions of disuse/mechanical unloading. Since low-dose lithium treatment has been shown to benefit the musculoskeletal system of mammals, this study sought to investigate the effects of subtherapeutic doses of lithium chloride (LiCl) on the functional properties and markers of protein metabolism in the postural soleus muscle of rats under 7 days of mechanical unloading. Rat hindlimbs were unloaded using the hindlimb suspension (HS) method. Male Wistar rats were divided into four groups: (1) vivarium control (C), (2) vivarium control + LiCl (C + Li), (3) 7-day HS (HS), and (4) 7-day HS + LiCl (HS + Li). LiCl (40 mg/kg) was administered intraperitoneally daily throughout the experiment. Structural changes in the muscle were studied using immunohistochemistry. Markers of anabolic and catabolic processes were assessed using Western blotting and PCR. LiCl treatment during HS prevented a decrease in muscle fiber size and slow-to-fast fiber transformation. Additionally, LiCl administration during HS increased the specific maximum force and passive tension of the soleus compared to the control. LiCl administration during HS prevented decreases in GSK-3β (Ser9) and 4E-BP1 (Thr37/46) phosphorylation. Furthermore, LiCl prevented the HS-induced increase in ubiquitin, atrogin-1, MuRF-1, ULK1, and calpain-1 mRNA expression. Thus, daily low-dose LiCl administration during 7-day HS maintained the morphological parameters and increased the functional properties of the rat soleus muscle. This was accompanied by the prevention of the activation of the key proteolytic markers in the soleus muscle.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":"478 1","pages":"1"},"PeriodicalIF":3.2,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on \"spatially dependent tissue distribution of thyroid hormones by plasma thyroid hormone binding proteins\".","authors":"Amnuay Kleebayoon, Viroj Wiwanitkit","doi":"10.1007/s00424-025-03111-6","DOIUrl":"10.1007/s00424-025-03111-6","url":null,"abstract":"","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1323-1324"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of intrarenal afferent stimulation by bradykinin on renal sympathetic nerve activity: tonic inhibition contributing to renal function.","authors":"Eva Hutter, Tilmann Ditting, Martin Hindermann, Karl F Hilgers, Roland E Schmieder, Christian Morath, Mario Schiffer, Kerstin Amann, Roland Veelken, Kristina Rodionova","doi":"10.1007/s00424-025-03116-1","DOIUrl":"10.1007/s00424-025-03116-1","url":null,"abstract":"<p><p>Bradykinin (BK) may increase renal sodium excretion by decreasing tubular ENaC activity. Afferent renal nerve activity (ARNA) putatively controls renal sympathetic nerve activity (RSNA) involved in renal sodium handling. We recently found tonic sympatho-inhibition due to intrarenal ARNA stimulation by the TRPV1 agonist capsaicin (CAP). Since BK is known to augment TRPV1 effects, we hypothesized that intrarenally applied BK also tonically inhibits RSNA. Four groups of rats (n = 8; BK, CAP, HOE + BK, NaCl-control) were equipped with arterial and venous catheters for blood pressure (BP) and heart rate (HR) recordings and drug application; bipolar electrodes for RSNA and ARNA recordings, renal arterial catheter for intrarenal administration (IRA) of bradykinin (BK: 10^-5 M, 20 µl and 10^-4 M; 2.5, 5, 10 µl), capsaicin (CAP 3.3, 6.6, 10 and 33*10^-7 M, 10 µl). The B2-receptor antagonist HOE-140 (10^-4 M, 40 µl) was administered intravenously (IV) just before IRA BK (HOE + BK), finally the NK₁-receptor blocker RP67580 (10^-2 M, 15 µl; IV) was applied in all groups at the end of the experiment. IRA BK and CAP momentarily increased ARNA. IRA CAP, IRA BK, and IRA HOE + BK, decreased RSNA from 4.2 ± 0.8 to 1.3 ± 0.2 µV*sec (BK, P < 0.01), 3.6 ± 0.5 to 0.9 ± 0.2 µV*sec (CAP, P < 0.01) and 3.2 ± 0.3 to 0.8 ± 0.1 µV*sec (HOE-BK, P < 0.01). Suppressed RSNA (BK, CAP, HOE + BK) was unmasked by IV RP67580: 1.6 ± 0.5 to 8.6 ± 2.9 µV*sec (BK, P < 0.01); 1.0 ± 0.2 to 6.1 ± 1.5 µV*sec (CAP, P < 0.01); 0.8 ± 0.2 to 4.5 ± 0.8 µV*sec (HOE-BK, P < 0.05). IRA BK was associated with momentary increases of RSNA, abolished by HOE-140. Intrarenal stimulation of renal afferent nerves by BK induced tonic renal sympathodepression likely augmenting sodium and water excretion.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1377-1389"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12640332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the relationship between intercostal muscle oxygenation measured by near-infrared spectroscopy and exercise capacity in group E COPD patients.","authors":"Felipe Contreras-Briceño, Maximiliano Espinosa-Ramírez","doi":"10.1007/s00424-025-03126-z","DOIUrl":"10.1007/s00424-025-03126-z","url":null,"abstract":"","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1325-1326"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acquired cystic fibrosis transmembrane conductance regulator dysfunction.","authors":"Michael Eisenhut, Helen Wallace","doi":"10.1007/s00424-025-03127-y","DOIUrl":"10.1007/s00424-025-03127-y","url":null,"abstract":"<p><p>Rhinorrhoea associated with viral upper respiratory tract infections or reactions to aeroallergens affects all human beings and has been associated with loss of quality of life. Pulmonary fluid accumulation in acute lung injury is a leading cause of hospital admission and death from lower respiratory tract infection and sepsis. In this review, the evidence is presented supporting the hypothesis that both rhinorrhoea and inflammation-related pulmonary fluid accumulation are primarily due to dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR). Novel concepts of the mechanisms involved are introduced. Upper airway as well as alveolar surface liquid volumes have been shown to be dependent on the function of the CFTR. Its function is regulated directly by cytokines and through increased hydrostatic tissue pressure secondary to vasodilatation in allergic inflammation and infection. Regulation of upper airway fluid secretion manifested in allergic inflammation and viral infections can be explained by CFTR activation through vasodilatation mediated by bradykinin and histamine, and CFTR upregulation by interleukin-4. Alveolar fluid accumulation is related to inactivation of CFTR through microRNA derived from genomic cytokine effects including interleukin-1, interleukin-8, transforming growth factor, and tumour necrosis factor. The role of concomitant sodium-potassium ATPase inhibition is explained. Therapeutic interventions including already known CFTR inhibitors for excessive fluid secretion in the upper respiratory tract and remedies for inflammation-induced fluid accumulation in the lower respiratory tract due to CFTR dysfunction using anti-inflammatory and CFTR activating agents are introduced.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1309-1322"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian adaptations to regular treadmill exercise alter temporal changes in dopamine and serotonin activity in brain areas.","authors":"Frederico Sander Mansur Machado, Nayara Abreu Coelho Horta, Quezia Teixeira Rodrigues, Thais Santana Rocha Cardoso, Nayara Soares Sena Aquino, Raphael Escorsim Szawka, Maristela Oliveira Poletini, Cândido Celso Coimbra","doi":"10.1007/s00424-025-03128-x","DOIUrl":"10.1007/s00424-025-03128-x","url":null,"abstract":"<p><p>The beneficial effects of regular exercise may be mediated through its regulatory influence on circadian rhythm. Here, we investigated how regular exercise training affects spontaneous locomotor activity (SLA) and body temperature (T-body) rhythms and its influence on temporal changes in brain dopaminergic and serotonergic activity, corticosterone levels, and muscle Per1 and Bmal1 gene expression. The aerobic exercise schedule consisted of five days of training in the light phase (Zeitgeber time = ZT4 to ZT6, with light on at 7:00 h) followed by a two-day recovery period. After 8 weeks, brain, blood, and muscle samples were collected from adult male rats. Dopamine (DA) and serotonin (5-HT) and their respective metabolites, DOPAC and 5-HIAA, were measured in microdissections of the caudate putamen (CP), preoptic area (POA), and the paraventricular nucleus of the hypothalamus (PVN). Exercise increased the SLA at the end of the night, delayed the acrophase, and increased the mesor of the SLA rhythm. No alterations were found in the T-body rhythm and corticosterone blood levels, although hyperthermia was observed after exercise sessions. Exercise increased muscle Per1 expression at ZT0, leading to a non-rhythmic profile in exercised animals. There were no changes in the CP dopaminergic and serotonergic activity, but there was a decrease in POA at ZT6 and an increase in PVN serotonergic activity at ZT0, resulting in a non-rhythmic profile in exercised animals. Thus, regular physical exercise during the light phase with alterations in SLA promotes adjustments in the daily oscillation in monoaminergic activity in areas directly involved in regulating daily T-body and SLA.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1391-1403"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the relationship between intercostal muscle oxygenation measured by near-infrared spectroscopy and exercise capacity in group E COPD patients.","authors":"Buğra Kerget","doi":"10.1007/s00424-025-03129-w","DOIUrl":"10.1007/s00424-025-03129-w","url":null,"abstract":"","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1327-1328"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial ATP synthase 8 single-nucleotide polymorphism affects oxidative stress and survival of mice.","authors":"Gesine Reichart, Johannes Mayer, Tursonjan Tokay, Timo Kirschstein, Falko Lange, Rüdiger Köhling","doi":"10.1007/s00424-025-03123-2","DOIUrl":"10.1007/s00424-025-03123-2","url":null,"abstract":"<p><p>Single-nucleotide polymorphisms in mitochondrial DNA (mtDNA) encoded genes of respiratory chain complexes are known to be associated with severe diseases and life-threatening syndromes. In the assembly of the ATP synthase, the enzyme that in the final steps of oxidative phosphorylation generates ATP from ADP and inorganic phosphate, two subunits (ATP6 and ATP8) are mtDNA-encoded. In our study, we investigated the impact of a single-nucleotide polymorphism in MT-ATP8 with respect to memory function in a preclinical model. Here, we have employed two conplastic mouse strains. The mouse strain C57BL/6 J-mt^AKR/J served as a control with wild-type sequence in MT-ATP8, while C57BL/6 J-mt^FVB/NJ exhibited an m.7778G > T transversion. Using two age groups (3 months and 24 months), levels of reactive oxygen species (ROS), spatial learning in the Morris-Water-Maze, and long-term potentiation were assessed. Immunohistologically, the expressions of NeuN and GFAP were quantified. Additionally, the lifespan of both strains was registered. In comparison to young C57BL/6 J-mt^FVB/NJ mice, aged animals had higher ROS levels in the hippocampus. A decreased NeuN/GFAP level was found in C57BL/6 J-mt^FVB/NJ mice as well as in old animals of the control strain. Aged animals performed worse in the swimming trials, but no significant differences between both strains were detected. The long-term potentiation recordings revealed reduced synaptic plasticity in young C57BL/6 J-mt^FVB/NJ mice. Interestingly, C57BL/6 J-mt^FVB/NJ mice presented an extended lifespan compared to animals of the control strain. Together, our data suggest a minor impact of a single-nucleotide polymorphism in MT-ATP8 on spatial learning and oxidative stress depending on the neuronal tissue. In line with the concept of mitohormesis, our findings may be linked to the longevity of mice harbouring single-nucleotide polymorphisms.</p>","PeriodicalId":19954,"journal":{"name":"Pflugers Archiv : European journal of physiology","volume":" ","pages":"1343-1357"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12640345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}