Pharmacological Reports最新文献

{"title":"Achieving target plasma concentrations of beta-lactam antibiotics in critically ill patients: a retrospective study of full-dose administration in the first 24 hours.","authors":"Milada Halačová, Marie Mieresová, Jan Kubele, Eva Klapková, Dalibor Černý, Petr Waldauf, František Duška","doi":"10.1007/s43440-026-00836-8","DOIUrl":"https://doi.org/10.1007/s43440-026-00836-8","url":null,"abstract":"","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rifaximin's therapeutic spectrum: approved indications and experimental insights into emerging uses.","authors":"Irene Palenca, Silvia Basili Franzin, Marcella Pesce, Giovanni Sarnelli, Giuseppe Esposito","doi":"10.1007/s43440-026-00830-0","DOIUrl":"https://doi.org/10.1007/s43440-026-00830-0","url":null,"abstract":"","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The extensive erythrocyte-plasma partitioning of trametinib - implications for pharmacokinetic studies and therapeutic drug monitoring.","authors":"Bence János Chriszt, Zoltán Köllő, Orsolya Geda, Éva Csöndör, Róbert Farkas, Barna Vásárhelyi, Miklós Garami, Gellért Balázs Karvaly","doi":"10.1007/s43440-026-00840-y","DOIUrl":"https://doi.org/10.1007/s43440-026-00840-y","url":null,"abstract":"","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146228025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trimetazidine modulates angiogenesis, inflammation, and metabolism-related gene expression to promote diabetic foot ulcer healing: a transcriptomic analysis.","authors":"Dorian Dulcic, Lucija Dumancic, Andrea Crkvenac Gregorek, Majda Vrkic Kirhmajer, Robert Likic","doi":"10.1007/s43440-026-00841-x","DOIUrl":"https://doi.org/10.1007/s43440-026-00841-x","url":null,"abstract":"<p><strong>Background: </strong>Diabetic foot ulcers (DFUs) are a chronic complication of diabetes associated with impaired healing and tissue ischemia.</p><p><strong>Aim: </strong>This study explores the transcriptional effects of trimetazidine (TMZ) in wound-relevant human cell models to generate hypotheses regarding its potential mechanisms of action.</p><p><strong>Methods: </strong>We conducted a secondary analysis of LINCS Phase 5 transcriptomic data to evaluate TMZ-induced gene expression in four human cell lines relevant to DFU pathophysiology: HUVEC (endothelial), THP1 (monocytic), A375 (keratinocytic-like), and MCF7 (epithelial-like).</p><p><strong>Results: </strong>TMZ induced 700-900 differentially expressed genes per cell line, with distinct yet convergent responses across models. A consistent activation of the PI3K-Akt signaling pathway was observed, along with modulation of noncanonical NF-κB components such as RELB and CXCL2. TMZ promoted metabolic reprogramming, including SIRT3 upregulation and glycolysis inhibition, and selectively regulated extracellular matrix remodeling genes. Importantly, pro-angiogenic and anti-inflammatory signatures emerged independently of VEGF, with notable downregulation of PTGS2 and TLR4.</p><p><strong>Conclusion: </strong>These results highlight TMZ's potential to modulate gene networks associated with angiogenesis, inflammation, and metabolism. The findings are exploratory and warrant validation in preclinical models to assess their therapeutic relevance.</p>","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin and quercetin alter mouse rod phototransduction kinetics in a manner consistent with phosphodiesterase-6 inhibition.","authors":"Lorenzo Cangiano, Claudia Gargini, Sabrina Asteriti","doi":"10.1007/s43440-026-00835-9","DOIUrl":"https://doi.org/10.1007/s43440-026-00835-9","url":null,"abstract":"","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex differences in the antidepressant-like response induced by the imidazoline-2 receptor compound 12d, a (3-phenylcarbamoyl-3,4-dihydro‑2H‑pyrrol-2-yl)phosphonate.","authors":"Elena Hernández-Hernández, Sandra Ledesma-Corvi, Fernando Yáñez-Gómez, Neus Mateu-Mercader, Andrea Bagán, Carmen Escolano, M Julia García-Fuster","doi":"10.1007/s43440-025-00810-w","DOIUrl":"10.1007/s43440-025-00810-w","url":null,"abstract":"<p><strong>Background: </strong>The synthesis of (3-phenylcarbamoyl-3,4-dihydro-2H-pyrrol-2-yl)phosphonates provided novel compounds with relevant affinities for imidazoline 2 (I2) receptors in human brain tissues. A selected compound, 12d, showed improved cognitive and analgesic properties at the preclinical level through the modulation of I2 receptors, but its potential innovative use as an antidepressant is still unknown.</p><p><strong>Methods: </strong>Male and female adult Sprague-Dawley rats were treated with 3 ip injections of compound 12d (10 or 20 mg/kg), or vehicle (1 ml/kg DMSO), 24, 5, and 1 h prior to scoring its antidepressant-like efficacy under the stress of the forced-swim test. Hippocampal neuroplasticity markers (i.e., FADD, p-ERK/ERK, mBDNF) were evaluated 24 h post-treatment (and post-forced-swim test exposure).</p><p><strong>Results: </strong>The novel results proved a sex-dependent efficacy of 12d, with dose-dependent antidepressant-like effects in adult male rats, and an inefficacious response for females. Moreover, compound 12d did not alter any of the hippocampal markers evaluated.</p><p><strong>Conclusions: </strong>These results presented 12d as a novel therapeutic antidepressant candidate at the tested conditions, although only for male rats, thus requiring further studies to better understand the observed sex disparities as well as its molecular underpinnings. Moreover, compound 12d joins the list of other I2 receptor ligands with known antidepressant-like efficacy, validating and strengthening this receptor as a target in this field for future drug development.</p>","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":"341-349"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel LC-MS/MS method for measuring methotrexate in high-dose therapy: a comparative study with commercial EMIT and EIA immunoassays.","authors":"Agnieszka Czajkowska, Aleksandra Mikulska, Martyna Poniewierska, Agnieszka Suchan, Maciej Sierakowski, Tomasz Pawiński, Arkadiusz Kocur","doi":"10.1007/s43440-025-00807-5","DOIUrl":"10.1007/s43440-025-00807-5","url":null,"abstract":"<p><strong>Background: </strong>Methotrexate (MTX) is a widely used chemotherapeutic agent in pediatric oncology, where high-dose protocols (HDMTX; ≥500 mg/m²) are standard for treating hematological and central nervous system malignancies. Due to its narrow therapeutic index and potential for severe toxicity, therapeutic drug monitoring (TDM) of plasma MTX concentrations is essential to guide leucovorin rescue therapy and prevent adverse effects.</p><p><strong>Methods: </strong>The presented study aimed to compare the analytical performance of two immunoassays-enzyme-multiplied immunoassay technique (EMIT) and enzyme immunoassay (EIA)-against a newly developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.</p><p><strong>Results: </strong>The LC-MS/MS assay demonstrated excellent linearity, sensitivity (LLOQ = 0.01 µmol/L), and precision, meeting ICH M10 regulatory guidelines. Clinical samples from pediatric patients receiving HDMTX were analyzed using all three methods. Results showed strong correlations (r > 0.93) between methods; however, immunoassays exhibited biases related to cross-reactivity with MTX metabolites such as DAMPA (2,4-diamino-N₁₀-methylpteroic acid) and 7-OH-MTX, which may lead to overestimation of MTX levels and unnecessary prolongation of leucovorin rescue.</p><p><strong>Conclusions: </strong>While immunoassays remain practical for routine monitoring due to their accessibility and speed, LC-MS/MS provides superior accuracy and should be the method of choice in critical clinical situations. These findings underscore the importance of selecting the appropriate assay to optimize HDMTX therapy and ensure patient safety.</p>","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":"327-340"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145549926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms and therapeutic strategies of GPX4 regulation in acute kidney injury.","authors":"Zhidan Shi, Chu Zhang, Tian Xie, Jie Song, Xiaoqian Zeng, Jiayi Hu, Xinqi He, Qingyang Zhang, Shuting Chen, Xinpeng Zhou, Guangzhe Yao, Ling He","doi":"10.1007/s43440-025-00777-8","DOIUrl":"10.1007/s43440-025-00777-8","url":null,"abstract":"","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":"218-235"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic modulation in cancer drug discovery: promising targets and clinical applications.","authors":"Sundaram Vickram, Shofia Saghya Infant, Manikandan Sivasubramanian, Saravanan Anbalagan, Mathan Muthu Chinnakannu Marimuthu, Hitesh Chopra","doi":"10.1007/s43440-025-00770-1","DOIUrl":"10.1007/s43440-025-00770-1","url":null,"abstract":"<p><p>Epigenetic modulation has emerged as a central strategy that can change the fate of tumour cells to offer more rational and precise approaches by modulating reversible changes in chromatin structure, regulating gene expression without altering DNA sequence. Many reports have indicated the contributions of abnormal epigenetic alterations, particularly DNA methylation and histone modification patterns, as well as their association with non-coding RNA interactions during cancer emergence, development or resistance to standard therapies. Ongoing studies on various inhibitors also demonstrate encouraging preclinical results and potent inhibitory activity. Furthermore, combining epigenetic medicines with conventional treatment approaches such as chemotherapy and radiotherapy is proven to improve therapeutic efficacy in resistant cases of various malignancies. This article also briefly reviews RNA modifications (epitranscriptomics, such as m6A and m5C), novel acetylation modifications, chromosomal interaction studies, and the role of AlphaFold. The present review further illustrates these translational challenges and future opportunities in epigenetic drug development, while shedding light on the necessity of developing predictive biomarkers capable of informing personalized therapies to reduce off-target effects. The ability to target epigenetic modulators has the potential to improve patient outcomes and increase treatment options when coupled with traditional guidelines, as evidenced by on-going clinical trials and FDA approvals.</p>","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":"45-64"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of machine learning in the evaluation of urinary tract infections incidence in patients in a Nursing and Treatment Facility.","authors":"Urszula Grzegorzek, Joanna Sobiak, Ewa Jaworucka, Bartosz Sznek, Andrzej Czyrski","doi":"10.1007/s43440-025-00804-8","DOIUrl":"10.1007/s43440-025-00804-8","url":null,"abstract":"<p><strong>Background: </strong>Urinary tract infection (UTI) is a serious problem in the healthcare system. It is caused by bacteria from the gastrointestinal tract. The risk factors that impact the UTI incidence include administration of certain drugs (flozins), sex, use of urinary catheter, and diabetes. This is a retrospective study of the records of 76 patients from a Nursing and Treatment Facility at County Hospital in Drezdenko (Poland) aimed to assess the factors that may have an impact on the incidence of UTI.</p><p><strong>Methods: </strong>The following factors were taken into consideration: dapagliflozin administration (yes/no), diabetes (yes/no), sex (male/female), kidney failure (yes/no), and use of urinary catheter (yes/no). The impact of the above variables on the UTI incidence was estimated using multivariate regression analysis and machine learning, such as logistic regression, artificial neural networks (ANN), and decision trees (recursive partitioning).</p><p><strong>Results: </strong>As revealed by the multivariate regression analysis, UTI was significantly affected only by dapagliflozin administration. The machine learning techniques showed greater sensitivity in detecting significant factors - dapagliflozin administration was identified as the most important one. Moreover, the logistic regression analysis also indicated sex (female). In the case of ANN and decision tree, the other significant factors, besides dapagliflozin intake, in the model were the use of a urinary catheter, sex (female), diabetes, and kidney failure (in descending importance). The variables were listed in the same order of descending importance for both the ANN and the decision tree.</p><p><strong>Conclusions: </strong>In the case of catheterized patients, the administration of flozins should be cautiously approached, as should the catheterization of patients taking flozins.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":" ","pages":"315-326"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145459443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}