Pharmaceutical Biology最新文献

{"title":"Notoginsenoside R1 improves intestinal microvascular functioning in sepsis by targeting Drp1-mediated mitochondrial quality imbalance.","authors":"Dongyao Hou, Ruixue Liu, Shuai Hao, Yong Dou, Guizhen Chen, Liangming Liu, Tao Li, Yunxing Cao, He Huang, Chenyang Duan","doi":"10.1080/13880209.2024.2318349","DOIUrl":"10.1080/13880209.2024.2318349","url":null,"abstract":"<p><strong>Context: </strong>Sepsis can result in critical organ failure, and notoginsenoside R1 (NGR1) offers mitochondrial protection.</p><p><strong>Objective: </strong>To determine whether NGR1 improves organ function and prognosis after sepsis by protecting mitochondrial quality.</p><p><strong>Materials and methods: </strong>A sepsis model was established in C57BL/6 mice using cecum ligation puncture (CLP) and an <i>in vitro</i> model with lipopolysaccharide (LPS, 10 µg/mL)-stimulated primary intestinal microvascular endothelial cells (IMVECs) and then determine NGR1's safe dosage. Groups for each model were: <i>in vivo</i>-a control group, a CLP-induced sepsis group, and a CLP + NGR1 treatment group (30 mg/kg/d for 3 d); <i>in vitro</i>-a control group, a LPS-induced sepsis group, and a LPS + NGR1 treatment group (4 μM for 30 min). NGR1's effects on survival, intestinal function, mitochondrial quality, and mitochondrial dynamic-related protein (Drp1) were evaluated.</p><p><strong>Results: </strong>Sepsis resulted in approximately 60% mortality within 7 days post-CLP, with significant reductions in intestinal microvascular perfusion and increases in vascular leakage. Severe mitochondrial quality imbalance was observed in IMVECs. NGR1 (IC₅₀ is 854.1 μM at 30 min) targeted Drp1, inhibiting mitochondrial translocation, preventing mitochondrial fragmentation and restoring IMVEC morphology and function, thus protecting against intestinal barrier dysfunction, vascular permeability, microcirculatory flow, and improving sepsis prognosis.</p><p><strong>Discussion and conclusions: </strong>Drp1-mediated mitochondrial quality imbalance is a potential therapeutic target for sepsis. Small molecule natural drugs like NGR1 targeting Drp1 may offer new directions for organ protection following sepsis. Future research should focus on clinical trials to evaluate NGR1's efficacy across various patient populations, potentially leading to novel treatments for sepsis.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"62 1","pages":"250-260"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139932376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Salvadora persica</i> leaves: phytochemical profile and <i>in vitro</i>-inhibitory activity on inflammatory mediators implicated in periodontal disease.","authors":"Sabine Kobetitsch, Barbara Gierlikowska, Olaf Kunert, Ahmed M A Mazen, Pia Raab, Nadine Kretschmer, Carina Donolo, Teresa Pirker, Rudolf Bauer, Anna K Kiss, Eva-Maria Pferschy-Wenzig","doi":"10.1080/13880209.2024.2374801","DOIUrl":"10.1080/13880209.2024.2374801","url":null,"abstract":"<p><strong>Context: </strong>Virtually all parts of <i>Salvadora persica</i> L. (Salvadoraceae) are used in traditional medicine. The twigs and leaves are used for oral health, but leaves are far less investigated.</p><p><strong>Objective: </strong>This study assesses the oral health-promoting potential of <i>S. persica</i> leaves with emphasis on anti-inflammatory and antiproliferative effects and provides an in depth-characterization of their metabolite profile.</p><p><strong>Materials and methods: </strong>Hot-water and methanolic <i>S. persica</i> leaf extracts (1, 10, and 100 µg/mL) and their major constituents (5, 10, and 50 µM), were subjected to cellular assays on IL-8 and TNFα release in LPS-stimulated human neutrophils, NO-release in LPS/IFNγ stimulated mouse macrophages, and proliferation of HNO97 human tongue carcinoma cells. Metabolite profiling was performed by UHPLC-HRMS analysis. Major constituents were isolated and structurally elucidated.</p><p><strong>Results and discussion: </strong>Both extracts showed pronounced anti-inflammatory activity in LPS-stimulated neutrophils. Major identified compound classes were flavonoid glycosides, the glucosinolate glucotropaeolin, phenyl- and benzylglycoside sulfates, and megastigmane glycosylsulfates, the latter ones identified for the first time in <i>S. persica</i>. Glucotropaeolin strongly inhibited the release of IL-8 and TNF-α (13.3 ± 2.0 and 22.7 ± 2.6% of the release of stimulated control cells at 50 µM), while some flavonoids and 3-(3'-<i>O</i>-sulfo-β-d-glucopyranosyloxy)-7,8-dihydro-β-ionone, a newly isolated megastigmane glycosylsulfate, were moderately active. Benzylisothiocyanate, which is likely formed from glucotropaeolin during traditional application of <i>S. persica,</i> showed considerable antiproliferative activity (IC₅₀ in HNO97 cells: 10.19 ± 0.72 µM) besides strongly inhibiting IL-8 and TNFα release.</p><p><strong>Conclusions: </strong>Glucotropaeolin and benzylisothiocyanate are likely implicated in the oral health-promoting effects of <i>S. persica</i> leaves. The chemistry and pharmacology of the newly identified megastigmane glycosylsulfates should be further evaluated.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"62 1","pages":"563-576"},"PeriodicalIF":3.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11259067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology","authors":"Feng Lv, Mei Sun, Chunmeng Qin, Dan Du, Xiangru Zheng, Wenjun Li","doi":"10.1080/13880209.2024.2382962","DOIUrl":"https://doi.org/10.1080/13880209.2024.2382962","url":null,"abstract":"In China, HUANGQI is widely used for the treatment of Alzheimer’s disease (AD). However, a comprehensive understanding of its mechanism of anti-AD effects is lacking.To explore the active ingredien...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"7 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peter Houghton (1947 – 2024) – in memoriam*","authors":"Michael Heinrich","doi":"10.1080/13880209.2024.2365210","DOIUrl":"https://doi.org/10.1080/13880209.2024.2365210","url":null,"abstract":"Published in Pharmaceutical Biology (Vol. 62, No. 1, 2024)","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"27 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141586711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment with onion bulb extract both prevents and reverses allergic inflammation in a murine model of asthma","authors":"Ahmed Z. El-Hashim, Maitham A. Khajah, Khaled Y. Orabi, Sowmya Balakrishnan, Hanan G. Sary, Ahmad M. Barakat","doi":"10.1080/13880209.2024.2335187","DOIUrl":"https://doi.org/10.1080/13880209.2024.2335187","url":null,"abstract":"Asthma presents a global health challenge. The main pharmacotherapy is synthetic chemicals and biological-based drugs that are costly, and have significant side effects. In contrast, use of natural...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"240 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140562950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Babaodan overcomes cisplatin resistance in cholangiocarcinoma via inhibiting YAP1","authors":"Jiong Li, Xiangjun Ma, Faying Xu, Yuanliang Yan, Weiqing Chen","doi":"10.1080/13880209.2024.2331060","DOIUrl":"https://doi.org/10.1080/13880209.2024.2331060","url":null,"abstract":"Cholangiocarcinoma with highly heterogeneous, aggressive, and multidrug resistance has a poor prognosis. Although babaodan (BBD) combined with cisplatin improved non-small cell lung cancer efficacy...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"58 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liuwei Dihuang pills attenuate ovariectomy-induced bone loss by alleviating bone marrow mesenchymal stem cell (BMSC) senescence via the Yes-associated protein (YAP)-autophagy axis","authors":"Bing Liang, Xiongbin Chen, Min Li, Lingling Zhang, Xia Yang, Liangqin Shi, Yanju Gong, Yuanyuan Gong, Huan Xu, Xiao Wu, Zhong Jin, Yanru Wang, Luwei Liu, Xiaohong Yi, Lushuang Xie, Hua Zhong, Chongyang Shen, Yong Wang, Lan Yang","doi":"10.1080/13880209.2023.2291675","DOIUrl":"https://doi.org/10.1080/13880209.2023.2291675","url":null,"abstract":"Liuwei Dihuang pill (LWDH) has been used to treat postmenopausal osteoporosis (PMOP).To explore the effects and mechanisms of action of LWDH in PMOP.Forty-eight female Sprague-Dawley rats were divi...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"33 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138741987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shenqi Fuzheng injection restores the sensitivity to gefitinib in non-small cell lung cancer by inhibiting the IL-22/STAT3/AKT pathway","authors":"Jiali Wang, Xianhai He, Zhirong Jia, Aiwen Yan, Kang Xiao, Shuo Liu, Mengjun Hou, Yaling Long, Xuansheng Ding","doi":"10.1080/13880209.2023.2292266","DOIUrl":"https://doi.org/10.1080/13880209.2023.2292266","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. Gefitinib is a first-line treatment for NSCLC. However, its effectiveness is hindered by the development of drug resistanc...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"31 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138741868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicotinamide mononucleotide alleviates endotoxin-induced acute lung injury by modulating macrophage polarization via the SIRT1/NF-κB pathway","authors":"Simeng He, Xianhong Jiang, Jing Yang, Ya Wu, Jia Shi, Xiaoyang Wu, Shihan Du, Yuan Zhang, Lirong Gong, Shuan Dong, Jianbo Yu","doi":"10.1080/13880209.2023.2292256","DOIUrl":"https://doi.org/10.1080/13880209.2023.2292256","url":null,"abstract":"Sepsis-induced acute lung injury (ALI) is a severe condition with limited effective therapeutics; nicotinamide mononucleotide (NMN) has been reported to exert anti-inflammatory activities.This stud...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"8 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138689936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis of research trends and topic areas in traditional Chinese medicine therapy for lymphoma","authors":"Gaofeng Zhang, Yanmei Qin, Shaobing Liu, Xi Chen, Wenwen Zhang","doi":"10.1080/13880209.2023.2288697","DOIUrl":"https://doi.org/10.1080/13880209.2023.2288697","url":null,"abstract":"Traditional Chinese Medicine (TCM) is effective as a cancer treatment modality. However, this is the first bibliometric analysis of TCM in lymphoma treatment.This study explores the current trends ...","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"15 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138630213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}