Pakistan Veterinary Journal最新文献_第8页

Effects of Oral Administration of Essential Oil (Mix Oil®) on Growth Performance and Intestinal Morphometry of Japanese Quails (Coturnix coturnix japonica) 口服精油(混合油®)对日本鹌鹑生长性能和肠道形态的影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.018

A. Masood

引用次数: 6

Sequestration and Histopathological Changes of the Lung, Kidney and Brain of Mice Infected with Plasmodium berghei that Exposed to Repeated Artemisinin 反复暴露于青蒿素对感染伯氏疟原虫小鼠肺、肾和脑的吸附及组织病理学改变

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.018

L. Maslachah

{"title":"Sequestration and Histopathological Changes of the Lung, Kidney and Brain of Mice Infected with Plasmodium berghei that Exposed to Repeated Artemisinin","authors":"L. Maslachah","doi":"10.29261/pakvetj/2019.018","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.018","url":null,"abstract":"Received: Revised: Accepted: Published online: February 03, 2018 June 12, 2018 September 30, 2018 January 28, 2019 The purpose of this study was to determine the pathogenesis of malarial infection in rodent as in vivo model in humans due to repeated exposure of artemisinin through organ histopathological picture. Healthy adult Albino swiss mice with average weight of 20-30 g were used for the study. Fifteen mice were divided into three groups: mice were infected with Plasmodium berghei which has been ever treated with artemisinin up to 4 times than treated by artemisinin (T4), infected mice with Plasmodium berghei which untreated by artemisinin as a control (C), infected mice with Plasmodium berghei which has been ever treated by artemisinin 4 times but untreated as a treatment control (TC). T4 group was oral administered with artemisinin which was given with \"4-day-test\" (4-DT) with ED99 dose (200 mg/kg weight of mice) for 3 days which begins 48 hours after infection but C and TC group were given aquadest. The histopathology of the lung, kidney and brain (cerebrum) was studied by routine histology method with Haematoxylin-Eosin staining. Histopathological parameters including edema, hemosiderin, thickened alveolar septa and inflammatory cell infiltration occurred in the lung. Cast formation, glomerulonephritis, tubular necrosis, and congestion could be seen in the cortex area of the kidney. The brain showed cerebral microvessels congested, hemorrhages and necrosis. In conclusion, repeated artemisinin exposure with repeated passages in mice cause increasing of sequestration on the lung and brain and increasing the histopathological changes of the lung, kidney and brain. ©2018 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47233440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pharmacokinetic Evaluation and Dosage Optimization of Ketoprofen in Healthy Beetal Goats 酮洛芬在健康山羊体内的药动学评价及用量优化

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.054

Zaka-ur-Rehman, M. Ashraf, Majeeda Rasheed

{"title":"Pharmacokinetic Evaluation and Dosage Optimization of Ketoprofen in Healthy Beetal Goats","authors":"Zaka-ur-Rehman, M. Ashraf, Majeeda Rasheed","doi":"10.29261/pakvetj/2019.054","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.054","url":null,"abstract":"Received: Revised: Accepted: Published online: February 19, 2019 March 04, 2019 March 12, 2019 April 06, 2019 Ketoprofen (KTP) is a frequently used non-steroidal anti-inflammatory drug (NSAID) in veterinary practice for the treatment of different inflammatory conditions. This work was carried out to evaluate the pharmacokinetic parameters of ketoprofen in healthy beetal goats. Eight goats were administered single intravenous (IV) dose of KTP (3.0mg/kg BW). Blood samples from all the goats were drawn before drug administration and then at different time intervals post administration. The KTP in plasma was estimated by using a high performance liquid Chromatography (HPLC) method. Mobile phase was mixture of phosphate buffer and acetonitrile (75:25 v/v). C18 column was used as stationary phase. The flow rate was 1.0ml/minute and temperature of column oven was adjusted to 30°C. Injection volume was 20μl. Wavelength was adjusted to 254nm. The pharmacokinetic parameters of KTP in beetal goats were calculated from plasma concentration-time data with APO MW/PHARM version 3.02 pharmacokinetic software using two compartmental model. The concentration of drug in plasma at different time intervals was calculated with regression/correlation analysis. All data are reported as mean ± SEM. The pharmacokinetic parameters determined are area under the curve (AUC) 7.711±0.60μg.h/ml, maximum concentration (Cmax) 13.64±0.98μg/ml, clearance (Cl) 0.325±0.02L/h/kg, volume of distribution (VD) 1.40±0.132L/kg, steady state volume of distribution (VDss) 0.50±0.09L/kg, half-life (t1/2) 3.10±0.37hrs, and elimination constant (Kel) 2.09±0.29L/hr. Results showed rapid elimination of ketoprofen from goat. Based on pharmacokinetics parameters, KTP at dosage of 2.47mg/kg BW is appropriate in beetal goats and may be repeated after 12 hours. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41326999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Tracing of Salmonella Contaminations Throughout an Integrated Broiler Production Chain in Dakahlia Governorate, Egypt 埃及Dakahlia省肉鸡生产链中沙门氏菌污染的追踪

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.038

M. Elsayed

{"title":"Tracing of Salmonella Contaminations Throughout an Integrated Broiler Production Chain in Dakahlia Governorate, Egypt","authors":"M. Elsayed","doi":"10.29261/pakvetj/2019.038","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.038","url":null,"abstract":"Received: Revised: Accepted: Published online: November 26, 2018 January 04, 2019 January 07, 2019 March 16, 2019 The data available regarding the production chain particularly in poultry flocks in Egypt are yet to be fully elucidated. The study aimed at providing an insight into the cross-contamination with Salmonella spp. at three different stages of an integrated broiler production chain. From 440 collected samples, 152 (34.5%) were positive for Salmonella spp. corresponded to samples collected from broiler farms (n=90, 40.9%), slaughter houses contact surfaces (n=12, 24%) and chicken carcass (n=50, 29.4%) in an integrated broiler supply chain. Isolation and identification of salmonella spp. were followed the standard procedures. Genomic bacterial DNA was extracted and confirmed using specific oligonucleotide primers sequences. Salmonella strains were classified into 11 serotypes. Nine different serotypes were found to contaminate thebroiler houses and flocks with the most prevalent serotypes S. Enteritidis (38.8%), S. Kentucky (23.3%) and S. Typhimurium (11.11%). The overall frequency of Salmonella contamination in the live broiler flocks was 40.9% with prevalence of 37.9, 60, 40, 53.3, 60 and 20% from cloacae swabs, litter, feed, water, farms walls and in the hand swabs from workers, respectively. The PCR products of four isolates, (two isolates per S. Typhimurium and S. Enteritidis) were identified by DNA sequencing to determine the potential genetic relationships in these isolates. The findings of the present study suggested that different Salmonella serotypes can persist along the production chain and that contamination could be linked to the contamination of the final product with a potential health hazard to consumers. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44689272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparative Association of slc11a1 (Nramp1) Gene with Susceptibility and Resistance to Brucellosis in Various Cattle and Buffaloes Breeds of Pakistan slc11a1 (Nramp1)基因与巴基斯坦不同品种牛和水牛对布鲁氏菌病的易感和抗性比较

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.091

R. Akhtar

{"title":"Comparative Association of slc11a1 (Nramp1) Gene with Susceptibility and Resistance to Brucellosis in Various Cattle and Buffaloes Breeds of Pakistan","authors":"R. Akhtar","doi":"10.29261/pakvetj/2019.091","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.091","url":null,"abstract":"Received: Revised: Accepted: Published online: February 19, 2019 June 20, 2019 July 03, 2019 August 15, 2019 It is observed that different Nramp1 genotypes are linked with Brucella resistance or susceptibility. So, current study aimed to find the Brucella resistance and susceptibility of local and exotic breeds of Pakistan on the basis of Nramp1 genotypes. Two breeds of buffaloes and five breeds of cattle were screened for brucellosis. The overall seropositivity determined by RBPT in cattle and buffaloes was 77.5% and 33.5% respectively. PCR results showed 54.5 and 25% brucella positive cases in cattle and buffaloes respectively. Nili-Ravi buffaloes pertain resistant genotype Nramp1 BB as compared to Kundi breeds of buffaloes possessed susceptible genotype Nramp1 AA. Similarly, Sahiwal breed was more resistant for brucellosis compared to cross breeds and Friesian. Phylogenetic analysis showed that Nramp1 gene of local breed had more closeness with B. taurus of China. This gene resistance can be used for genomic selection of animals to reduce disease cost and economic losses due to brucellosis. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42305100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Efficacy of Three Different Peste des Petits Ruminants (PPR) Vaccines and Determination of Appropriate Age of PPR Vaccination in Lambs 三种小反刍兽疫疫苗的效果及适宜接种年龄的确定

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.063

H. Irshad, Aman Ullah, A. Ahsan, A. B. Zahur, M. J. Basra, Mehreen Azhar, M. Afzal, M. Jahangir

{"title":"Efficacy of Three Different Peste des Petits Ruminants (PPR) Vaccines and Determination of Appropriate Age of PPR Vaccination in Lambs","authors":"H. Irshad, Aman Ullah, A. Ahsan, A. B. Zahur, M. J. Basra, Mehreen Azhar, M. Afzal, M. Jahangir","doi":"10.29261/pakvetj/2019.063","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.063","url":null,"abstract":"Received: Revised: Accepted: Published online: January 08, 2019 March 08, 2019 March 20, 2019 April 23, 2019 The study evaluated the efficacy of three different Peste des petits ruminants (PPR) vaccines and assessed the appropriate age of vaccination in lambs born without maternal antibodies at Livestock Experimental Station, Rakh Ghulaman. The lambs (n=94) were divided into ten groups. The lambs in Group A1 (1-<2 month), A2 (2-3 months) and A3 (>3 months) were vaccinated with PPR vaccine from company A and the lambs in Group B1 (1-<2 month), B2 (2-3 months) and B3 (>3 months) were vaccinated with PPR vaccine from company B. However, PPR vaccine from company C was given to four groups of lambs (Group C1, C2, C3 and C4). Serum samples were collected from experimental animals at monthly intervals for five months. Sera were analyzed using c-ELISA. The results indicated that most appropriate age of vaccination against PPR in lambs without maternal antibodies is 2-3 months with any of three vaccines. However, vaccine C may also be used at the age of < 1 month to get protection against PPR. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49533566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gross Morphological and Ontological Studies on Pituitary of Camel (Camelus dromedarius) 骆驼垂体的大体形态学和本体学研究

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.116

S. Jaspal, Zia-ur-Rahman, A. M. Cheema

{"title":"Gross Morphological and Ontological Studies on Pituitary of Camel (Camelus dromedarius)","authors":"S. Jaspal, Zia-ur-Rahman, A. M. Cheema","doi":"10.29261/pakvetj/2019.116","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.116","url":null,"abstract":"Received: Revised: Accepted: Published online: March 24, 2018 May 17, 2019 October 22, 2019 November 11, 2019 Thirty normal male and female camels (Camelus dromedarius) with three age groups (2-4, 5-10 and 11-onward years) were selected for pituitary gross morphological and ontological studies. Body weight and pituitary were recorded; Mean body weight of camels and mean relative pituitary gland weight as a percent of body weight were calculated. In camels of age greater than 11 years, body weight significantly correlated with relative pituitary weight (r=-0.754*), overall correlation of female body weight and pituitary weight irrespective of their age was highly significant (r=0.888**). Whereas, the body weight of female camel between 5-10 years of age reflected a significant correlation with relative pituitary weight (r=0.882*). The cells of adenohypophysis were arranged in the cluster and formed the pars distalis and pars intermedia. Overall acidophil in the pituitary of camels were higher than chromophobes and basophils while basophils were the lowest in number as compared to other two types of cells. It is concluded that body weight of camel is suggestive of its correlation with pituitary weight, sex and age. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49540621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions 应用葡萄籽提取物下调CA2基因增强肝功能修复胫骨软骨发育不良鸡

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.076

Hammad Qamar

引用次数: 5

Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size 镍纳米颗粒对大鼠肾毒性的影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.106

S. Z. Abdulqadir

{"title":"Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size","authors":"S. Z. Abdulqadir","doi":"10.29261/pakvetj/2019.106","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.106","url":null,"abstract":"Received: Revised: Accepted: Published online: October 21, 2018 May 28, 2019 August 21, 2019 October 15, 2019 Since nickel compounds are carcinogenic and strong toxicants to the various organs, it is necessary to carry out extra in vivo trials on Nickel nanoparticles (NiNPs) to demonstrate their influences on health pragmatically. The current study was executed to scrutinize the expected undesired impacts of various sizes of NiNPs on renal nephrons of rats. To meet the trial requirements, a total of 24 male Wistar rats, each 12 weeks old were allocated randomly into four groups (n=6). Group 1 was designed as the control (only given sodium chloride 0.9%) while the other three groups (2, 3 and 4) of the experimental animals were exposed to intraperitonial NiNPs (20mg/kg B.W) daily at three sizes (20 nm, 40 nm and 70 nm) for 28 days. Inflammatory cells aggregations of infiltrated leucocytes and degeneration of the proximal tubular cells were the most frequent histopathological features in the NiNPs treated groups which indicates a NiNPs-induced nephrotoxicity. Biochemical analysis of tissue malondialdehyde (MDA), superoxide dismutase (SOD) and serum creatinine were performed. MDA level was significantly elevated (P≤0.05) in all NiNPs treated groups as compared to the control group. All the three NiNPs groups revealed a significant tissue SOD and serum creatinine elevation as compared to the control group (P≤0.05). Furthermore, a significant increase in the p53 positive kidney tubular cells was detected in NiNPs treated groups as compared to control group (P≤0.05). All alterations above in the treated rats were size dependent; the smallest NiNPs being more toxic than the largest ones. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44603790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Protective Effect of Yeast Sludge and Whey Powder against Ochratoxicosis in Broiler Chicks 酵母泥和乳清粉对肉鸡赭石中毒的保护作用

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.077

H. Mujahid

引用次数: 10