Pharmacokinetic Evaluation and Dosage Optimization of Ketoprofen in Healthy Beetal Goats

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI:10.29261/pakvetj/2019.054

Zaka-ur-Rehman, M. Ashraf, Majeeda Rasheed

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引用次数: 2

Abstract

Received: Revised: Accepted: Published online: February 19, 2019 March 04, 2019 March 12, 2019 April 06, 2019 Ketoprofen (KTP) is a frequently used non-steroidal anti-inflammatory drug (NSAID) in veterinary practice for the treatment of different inflammatory conditions. This work was carried out to evaluate the pharmacokinetic parameters of ketoprofen in healthy beetal goats. Eight goats were administered single intravenous (IV) dose of KTP (3.0mg/kg BW). Blood samples from all the goats were drawn before drug administration and then at different time intervals post administration. The KTP in plasma was estimated by using a high performance liquid Chromatography (HPLC) method. Mobile phase was mixture of phosphate buffer and acetonitrile (75:25 v/v). C18 column was used as stationary phase. The flow rate was 1.0ml/minute and temperature of column oven was adjusted to 30°C. Injection volume was 20μl. Wavelength was adjusted to 254nm. The pharmacokinetic parameters of KTP in beetal goats were calculated from plasma concentration-time data with APO MW/PHARM version 3.02 pharmacokinetic software using two compartmental model. The concentration of drug in plasma at different time intervals was calculated with regression/correlation analysis. All data are reported as mean ± SEM. The pharmacokinetic parameters determined are area under the curve (AUC) 7.711±0.60μg.h/ml, maximum concentration (Cmax) 13.64±0.98μg/ml, clearance (Cl) 0.325±0.02L/h/kg, volume of distribution (VD) 1.40±0.132L/kg, steady state volume of distribution (VDss) 0.50±0.09L/kg, half-life (t1/2) 3.10±0.37hrs, and elimination constant (Kel) 2.09±0.29L/hr. Results showed rapid elimination of ketoprofen from goat. Based on pharmacokinetics parameters, KTP at dosage of 2.47mg/kg BW is appropriate in beetal goats and may be repeated after 12 hours. ©2019 PVJ. All rights reserved

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酮洛芬在健康山羊体内的药动学评价及用量优化

接收：修订：接受：在线发布：2019年2月19日2019年3月4日2019年4月12日酮洛芬（KTP）是兽医实践中常用的非甾体抗炎药（NSAID），用于治疗不同的炎症状况。本工作旨在评估酮洛芬在健康甜菜山羊体内的药代动力学参数。8只山羊接受了单次静脉注射（IV）剂量的KTP（3.0mg/kg体重）。在给药前抽取所有山羊的血样，然后在给药后的不同时间间隔抽取血样。采用高效液相色谱法测定血浆KTP。流动相为磷酸盐缓冲液和乙腈（75:25v/v）的混合物。C18柱为固定相。流速为1.0ml/分钟，柱烘箱温度调节至30°C。注射量为20μl。将波长调整为254nm。根据血浆浓度-时间数据，使用APO MW/PHARM 3.02版药代动力学软件，使用双室模型计算KTP在甜菜山羊中的药代动力学参数。通过回归/相关分析计算不同时间间隔的血浆中药物浓度。所有数据均为平均值±SEM。确定的药代动力学参数为曲线下面积（AUC）7.711±0.60μg.h/ml，最大浓度（Cmax）13.64±0.98μg/ml，清除率（Cl）0.325±0.02L/kg，分布体积（VD）1.40±0.132L/kg，稳态分布体积（VDss）0.50±0.09L/kg，半衰期（t1/2）3.10±0.37hrs，消除常数（Kel）为2.09±0.29L/hr。根据药代动力学参数，2.47mg/kg体重的KTP在甜菜山羊中是合适的，并且可以在12小时后重复。©2019 PVJ。保留所有权利

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.