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Basic Analysis of Glycolysis in Cardiac Tissue in Broiler Chickens Presenting with Ascites Syndrome 肉鸡腹水综合征心脏组织糖酵解的基本分析
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.060
Yaming Ge
{"title":"Basic Analysis of Glycolysis in Cardiac Tissue in Broiler Chickens Presenting with Ascites Syndrome","authors":"Yaming Ge","doi":"10.29261/pakvetj/2020.060","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.060","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Characterization of Infectious Laryngotracheitis Virus Isolates from Turkey by Molecular and Sequence Analysis 土耳其传染性喉气管炎病毒分离株的分子和序列分析
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.051
K. Can-Sahna
{"title":"Characterization of Infectious Laryngotracheitis Virus Isolates from Turkey by Molecular and Sequence Analysis","authors":"K. Can-Sahna","doi":"10.29261/pakvetj/2020.051","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.051","url":null,"abstract":"Received: Revised: Accepted: Published online: February 22, 2020 April 27, 2020 May 12, 2020 June 08, 2020 Infectious laryngotracheitis (ILT) is an economically important respiratory disease affecting the poultry industry worldwide. The aim of this study was to characterize the Turkish ILT virus (ILTV) isolates by sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ILTV isolates obtained from laying hens in Turkey in 2003 and 2018 were used in this study. The isolates were analyzed for ICP4, gG, gE and TK gene regions by PCR. The amplification products were used in RFLP analysis to determine the differences among the isolates. Sequence analyses of TK and ICP4 regions were carried out and a phylogenetic tree was formed by using the Maximum Likelihood method. Nucleotide identity values were then calculated among five isolates and other strains/isolates in Genbank. In addition, about 200 amino acid sequences of the start and end regions of the ICP4 gene were compared to other strains in Genbank. PCR-RFLP analysis indicated that Turkish ILTV isolates were low-virulent. In general, the nucleotide sequence similarities of the TK and ICP4 gene regions among Turkish isolates and others was more than 95% (lower in some Egyptian and Bangladeshi strains, 41 and 45% respectively); in the amino acid sequence, it was close to 100%. As a result, PCR-RFLP results were similar in many gene regions. However, evolutionary analysis of ICP4 and TK gene regions did not yield reliable results based on geographic distribution or pathogenicity levels. For this purpose, different methods, such as Bayesian analysis or the involvement of samples from different gene regions can yield more reliable results, just like whole-genome sequences. ©2020 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Evaluation of Passive Immunity Transfer against G6P[1] Rotavirus in Holstein Calves by ELISA ELISA法评价荷斯坦犊牛对G6P[1]轮状病毒的被动免疫转移
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.031
T. G. Rocha
{"title":"Evaluation of Passive Immunity Transfer against G6P[1] Rotavirus in Holstein Calves by ELISA","authors":"T. G. Rocha","doi":"10.29261/pakvetj/2020.031","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.031","url":null,"abstract":"The main strategy to prevent bovine rotavirus group A (RVA) diarrhea in calves is to vaccinate late-term dams aiming to enhance passive immunity transfer of specific immunoglobulins against the virus This study aimed to evaluate influence of parity in titers of immunoglobulin G (IgG), IgG1 and IgM in serum and colostrum of vaccinated or unvaccinated Holstein cows and in serum of its calves, associated with monitoring for RVA diarrhea in calves Cows and its calves were allotted into groups according to parity and vaccination (primiparous/multiparous;vaccinated/unvaccinated) and serum and colostrum samples of cows were taken as well as serum and fecal samples of its calves Parturition influenced colostral titers of IgG and IgG1, which were higher in multiparous cows, whilst IgM titers were influenced by vaccination, being higher in colostrum of vaccinated dams Lowest serum titers of IgG and IgG1 were found in calves born to unvaccinated primiparous dams Eleven calves presented RVA diarrhea, and genotypes G6P[11] and G6P[5] were found in the vaccinated and unvaccinated herds, respectively Vaccination of dams prolongs humoral immunity in calves and enhances colostrum quality and should be a primary concern in primiparous cows (C) 2020 PVJ All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Molecular Epidemiology and Characteristics of Streptococcus agalactiae Isolated from Bovine Mastitis in Large Dairy Herds of China 中国大型奶牛乳腺炎无乳链球菌分离株的分子流行病学及特征
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.025
Yang XueYun, Wang DengFeng, Li Jianjun, Meng XiaoXiao, Wei YuRong, Tuerxun Gunuer, Jin Yinghong, Xue Jing, Tariq Ali, Han Bo, Jia Bin
{"title":"Molecular Epidemiology and Characteristics of Streptococcus agalactiae Isolated from Bovine Mastitis in Large Dairy Herds of China","authors":"Yang XueYun, Wang DengFeng, Li Jianjun, Meng XiaoXiao, Wei YuRong, Tuerxun Gunuer, Jin Yinghong, Xue Jing, Tariq Ali, Han Bo, Jia Bin","doi":"10.29261/pakvetj/2020.025","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.025","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
ESBL-Producing Escherichia coli from Bovine Mastitis Induced Apoptosis of Bovine Mammary Epithelial Cells Via Alteration of ROS/MMP/bax/bcl-2 Signaling Pathway 产esbl的牛乳腺炎大肠杆菌通过改变ROS/MMP/bax/bcl-2信号通路诱导牛乳腺上皮细胞凋亡
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.038
Yuxiang Shi
{"title":"ESBL-Producing Escherichia coli from Bovine Mastitis Induced Apoptosis of Bovine Mammary Epithelial Cells Via Alteration of ROS/MMP/bax/bcl-2 Signaling Pathway","authors":"Yuxiang Shi","doi":"10.29261/pakvetj/2020.038","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.038","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Single Nucleotide Polymorphisms in the Promoter of CD4 Gene are Associated with Production and Mastitis Traits in Dairy Cattle 奶牛CD4基因启动子单核苷酸多态性与奶牛产量和乳腺炎性状相关
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.057
Arsalan Rasheed
{"title":"Single Nucleotide Polymorphisms in the Promoter of CD4 Gene are Associated with Production and Mastitis Traits in Dairy Cattle","authors":"Arsalan Rasheed","doi":"10.29261/pakvetj/2020.057","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.057","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Detection of Genetic Variations in Pattern Recognition Receptors (PRRs) Gene of Tharparkar Cattle Breed 塔帕卡牛模式识别受体(PRRs)基因遗传变异的检测
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.032
L. Iqbal
{"title":"Detection of Genetic Variations in Pattern Recognition Receptors (PRRs) Gene of Tharparkar Cattle Breed","authors":"L. Iqbal","doi":"10.29261/pakvetj/2020.032","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.032","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Occurrence and Molecular Characterization of Shiga Toxin-Producing Escherichia coli Isolates Recovered from Cattle and Goat Meat Obtained from Retail Meat Shops in Rawalpindi and Islamabad, Pakistan 巴基斯坦拉瓦尔品第和伊斯兰堡零售肉店牛肉和羊肉中产志贺毒素大肠杆菌分离株的发生及分子特征
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.045
H. Irshad
{"title":"Occurrence and Molecular Characterization of Shiga Toxin-Producing Escherichia coli Isolates Recovered from Cattle and Goat Meat Obtained from Retail Meat Shops in Rawalpindi and Islamabad, Pakistan","authors":"H. Irshad","doi":"10.29261/pakvetj/2020.045","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.045","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Prevalence of Antibiotic-Resistant and Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Chicken Meat from Eastern Turkey 产广谱β -内酰胺酶的耐药大肠杆菌在东土耳其鸡肉中的流行
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.047
A. Baran
{"title":"Prevalence of Antibiotic-Resistant and Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Chicken Meat from Eastern Turkey","authors":"A. Baran","doi":"10.29261/pakvetj/2020.047","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.047","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Developing a Novel, Efficient and Cost Effective Tissue Injury Model (in-vitro) on Equine Tendon Fibroblasts 建立一种新颖、高效、经济的马肌腱成纤维细胞体外组织损伤模型
IF 2.3 3区 农林科学
Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.058
Somia Shehzadi
{"title":"Developing a Novel, Efficient and Cost Effective Tissue Injury Model (in-vitro) on Equine Tendon Fibroblasts","authors":"Somia Shehzadi","doi":"10.29261/pakvetj/2020.058","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.058","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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