埃及牛圆环虫细胞色素B基因突变与Buparvaquone抗性

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI:10.29261/pakvetj/2020.006

S. Yousef, F. A. E. Balkemy, Hend M. El Damaty

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引用次数: 5

摘要

接收日期：修订日期：接受日期：在线发布日期：2019年10月5日2019年12月6日2019年2月19日2020年1月21日Buparvaquone现在是治疗热带泰瑞病的主要药物。目前的研究是为了确定在埃及条件下，牛对布帕奎酮的耐药性与环状泰勒菌（T.annulata）细胞色素b（Cyto b）基因突变之间的潜在联系。我们对从1头对布帕奎酮治疗有反应和5头对布帕奎酮治疗无反应的牛身上采集的6份血液样本中的环纹T.annulata的Cyto b基因进行了测序。分析显示，在五个耐药分离株的喹啉氧化（Qo2）药物结合位点内，密码子253和262发生了两个突变。Qo1位点的两个突变仅在布帕奎酮敏感分离株中同时发现。我们的研究结果表明，布帕奎酮治疗的失败可能归因于环纹T.Cyto b基因Qo2位点存在单点或双点突变。©2019 PVJ。保留所有权利

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Mutations in Theileria Annulata Cytochrome B Gene Associated with Buparvaquone Resistance in Cattle, Egypt

Received: Revised: Accepted: Published online: October 05, 2019 December 06, 2019 December 19, 2019 January 21, 2020 Buparvaquone is now the principle medicinal drug utilized against tropical theileriosis. The current study was conducted to determine the potential association between the resistance against buparvaquone and the occurrence of mutations in the Cytochrome b (Cyto b) gene of Theileria annulata (T. annulata) in cattle under Egyptian conditions. We sequenced the Cyto b gene of T. annulata of 6 blood samples collected from 1 responding and 5 non-responding cattle to buparvaquone treatment. The analysis revealed that two mutations occurred in codons 253 and 262 within the quinol oxidation (Qo2) drug-binding site of the five resistant isolates. Two mutations in the Qo1 site were found together in the buparvaquone sensitive isolate only. Our findings suggest that the failure of buparvaquone therapy could be attributed to the presence of single or double point mutations at the Qo2 site of T. annulata Cyto b gene. ©2019 PVJ. All rights reserved

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.