Pakistan Veterinary Journal最新文献_第6页

In vitro Experiments Revealed the Anthelmintic Potential of Herbal Complex against Haemonchus contortus 体外实验显示中药复合物对弯血螨有驱虫作用

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.128

M. Zaman

引用次数: 8

Molecular Characterization of Extensively Drug-resistant Pasteurella multocida Isolated from Apparently Healthy and Diseased Chickens in Egypt 埃及表面健康鸡和病鸡中广泛耐药多杀性巴氏杆菌的分子特征

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.020

Radwa A Elalamy

引用次数: 8

Morphofunctional Assessment of the Glycyrrhizinic Acid Effect on Myocardium of Rats under Adrenaline Loading 甘草酸对肾上腺素负荷大鼠心肌的形态功能影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.024

G. Bazekin

引用次数: 2

Effect of Ethanol Extract and Fractions of Physalis Calyx Seu Fructus on Inflammation and Mycoplasma Gallisepticum Physalis Calyx Seu fruct乙醇提取物及提取物对炎症及鸡败支原体的影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.036

Yanyan Yi

引用次数: 5

Isolation and Genetic Characterization of Staphylococcus haemolyticus from Cats 猫源溶血葡萄球菌的分离及遗传特性分析

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.033

K. Bierowiec

{"title":"Isolation and Genetic Characterization of Staphylococcus haemolyticus from Cats","authors":"K. Bierowiec","doi":"10.29261/pakvetj/2020.033","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.033","url":null,"abstract":"Received: Revised: Accepted: Published online: November 12, 2019 January 03, 2020 January 15, 2020 April 07, 2020 Staphylococcus haemolyticus is widespread in humans and animals. The aim of this study was to characterize S. haemolyticus isolates from sick and healthy cats according to their antibiotic properties and biofilm formation. A total of 80 S. haemolyticus isolates from 36 healthy and 20 sick cats were investigated. All the isolates from the sick as well as from the healthy animals were multidrug resistant at the genetic level, whereas 82.5% showed phenotypic resistance. Almost all the isolates were methicillin-resistant S. haemolyticus (MRSH) at the genotypic and phenotypic levels of 98.75% and 96.25%, respectively. Moreover, the most frequently observed phenotypic resistances were those for erythromycin (87.5%) and penicillin (93.75%), and the isolates harboured genes involved in resistance to penicillin – blaZ (97.5%), aminoglycosides – aac(6’)Ie-aph(2”)Ia (92.5%), macrolide-lincosamide-streptogramin – ermB (98.75%) and ermC (72.5%), and tetracyclines – tet(K) (98.75%) and tet(M) (100%). The percentage of biofilmpositive strains in healthy and sick cats amounted to 92.68% and 97.44% for the microtiter plate test (P=0.4962) and 71.17% and 56.41% for the Congo red agar assay (P=0.5164), respectively. Statistically significant associations were observed when the owner’s occupation was connected with healthcare, both human or veterinary, there was previous hospitalization of the owner, or cats were kept with other animals in the household. MRSH strains isolated from companion animals were frequently multidrug resistant, although they remained susceptible to antibiotics used in mainly human medicine. ©2020 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Serological Study for the Detection of Antibodies against leptospira in Goats 山羊钩端螺旋体抗体检测的血清学研究

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.021

M. U. Aziz

引用次数: 2

Gene Targeted Sequencing Analysis of Mycoplasma gallisepticum Strains in Poultry Flocks from Middle East and South Asia 中东和南亚地区鸡脓毒支原体基因靶向测序分析

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.023

V. Felice

{"title":"Gene Targeted Sequencing Analysis of Mycoplasma gallisepticum Strains in Poultry Flocks from Middle East and South Asia","authors":"V. Felice","doi":"10.29261/pakvetj/2020.023","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.023","url":null,"abstract":"The aim of this study was to attempt the molecular characterization of Mycoplasma gallisepticum (MG) strains detected in chicken flocks from United Arab Emirates (UAE), Saudi Arabia (SAU) and Sri Lanka (SL). Between 2012 and 2015, 55 samples from one layer flock from UAE, three breeder flocks from SAU and one broiler flock from SL were screened for MG using a pr- mgc 2 PCR; the positive samples were characterized by the gene-targeted sequencing (GTS) analysis. BLAST comparisons, followed by phylogenetic analysis, was carried out to compare the obtained sequences against published vaccine and field strain sequences. Fifty of the fifty-five (91%) examined samples, were MG positive by pr- mgc 2 PCR. The sequences detected in the samples from ts-11 vaccinated breeders from SAU showed 100% identity with the ts-11 vaccine sequences. The sequences detected in the samples from layers in UAE, were attributed to field strains, clustering together with Israeli MG strains. The sequences obtained from the samples from Sri Lanka broilers clustered separately from USA, Israeli, Australian, vaccine and reference strains. A potential route of MG field infection to the examined flocks may have been through the movement of birds, either directly (flock-to-flock transmission) or indirectly (through wild bird transmission). To our knowledge, this study reports the first molecular characterization by gene-targeted sequencing (GTS) analysis of MG strains from commercial poultry in SAU, UAE and SL.","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversified Epidemiological Pattern and Antibiogram of mecA Gene in Staphylococcus aureus Isolates of Pets, Pet Owners and Environment 宠物、宠物主人及环境金黄色葡萄球菌mecA基因的流行病学特征及抗生素谱分析

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.039

M. Shoaib

引用次数: 16

Serum Anti-Mullerian Hormone Profile from 10 Days of Age to Puberty and Its Relationship with Serum Testosterone and Estradiol Concentrations in Beetal Goat Kids 山羊10日龄至青春期血清抗苗勒管激素水平及其与血清睾酮和雌二醇浓度的关系

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.041

M. Ashraf

{"title":"Serum Anti-Mullerian Hormone Profile from 10 Days of Age to Puberty and Its Relationship with Serum Testosterone and Estradiol Concentrations in Beetal Goat Kids","authors":"M. Ashraf","doi":"10.29261/pakvetj/2020.041","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.041","url":null,"abstract":"Received: Revised: Accepted: Published online: December 4, 2019 March 09, 2020 March 18, 2020 May 04, 2020 The current study aimed to define the serum profile of anti-mullerian hormone (AMH), testosterone and estradiol (E2) concentrations in Beetal goat kids from 10 days of age to puberty (175±12.6 days). The study was conducted from July 2018 to January 2019. Blood samples were collected from male kids (n=26) at 10 days interval from 10 days of age to puberty for measurement of AMH, testosterone and E2 concentrations. The body weight of all kids was measured at 10 days of age and then on monthly basis till puberty. The results showed that the mean body weight at 10 d after birth was 7.04±0.86 kg, and significantly increased till the onset of puberty (36.4±4.49 kg). The minimal plasma AMH concentration was seen at 10 d, gradually increased to its peak level (P<0.05) at 40 d and 100 d and then declined steadily till puberty. The serum testosterone level increased non-significantly from day 10 to day 50 and then gradually increased to its peak level at 170 and 180d (P<0.05). The serum E2 concentration was low at 10 days of age (5.76±0.78 pg/ml), increased to its peak level at 60d (74.3±6.26 pg/ml; P<0.05 compared with 10d), then declined till puberty. The serum AMH at early pre-pubertal age (40, 50, 70 and 80d) was positively correlated with pubertal age around 180d (r=0.99, 0.95, 0.99 and 0.94), whereas testosterone concentration was negatively correlated with serum AMH level at 60d, while positively correlated at 150 and 180d. In conclusion, goat kids exhibit a characteristic serum AMH profile along with testosterone and E2 during pre-pubertal life, such that serum AMH at an early pre-pubertal age could be used as a biomarker for predicting initiation of sexual behavior and onset of puberty. ©2020 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69869758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Screening of Resistant and Virulent Genes in Salmonella Enteritidis and Salmonella Typhimurium from Poultry in Khyber Pakhtunkhwa 开伯尔-普赫图赫瓦省家禽肠炎沙门氏菌和鼠伤寒沙门氏菌耐药和毒力基因的分子筛选

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.044

Yasin Ahmad

引用次数: 3