Pakistan Veterinary Journal最新文献_第5页

Evaluation of Immunogenicity and Protective Efficacy of Eimeria maxima Immune Mapped Protein 1 with EDA Adjuvant in Chicken EDA佐剂对最大艾美耳球虫免疫定位蛋白1的免疫原性和保护作用的评价

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2020.043

X. Lin

{"title":"Evaluation of Immunogenicity and Protective Efficacy of Eimeria maxima Immune Mapped Protein 1 with EDA Adjuvant in Chicken","authors":"X. Lin","doi":"10.29261/pakvetj/2020.043","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.043","url":null,"abstract":"Received: Revised: Accepted: Published online: December 15, 2018 December 12, 2019 May 03, 2020 May 06, 2020 Immune mapped protein-1 (IMP1) has been shown as a protective protein associated with Eimeria (E.) maxima and cellular fibronectin extra domain A (EDA), the ligand of toll-like receptor 4 (TLR4), has the potential to be used as molecular adjuvant of the vaccine. In the present study, we estimated the protective efficacy of a subunit vaccine comprising of EDA and EmIMP1 against E. maxima challenges. For this purpose, the fusion protein EmIMP1-EDA was thoroughly purified by using over-expression of replicated E. coli of the protein. Chickens were immunized by the EmIMP1 vaccine having Freund’s adjuvant or with the EmIMP1-EDA vaccine without adjuvant. The immunization of chicken by the EmIMP1-EDA vaccine produced stronger IFN-γ secretion as compared to that in other groups (P<0.05). The result of the EmIMP1EDA vaccine was more pronounced on the parameters including weight gain, oocyst shedding, and lesion scores as compared to the EmIMP1 vaccine employing Freund’s adjuvant, but there were no statistical differences between the two groups (P>0.05). These results showed that EmIMP1-EDA fusion protein could be used as a potent subunit vaccine against E. maxima infection. ©2020 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47486879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Phytochemical Analysis and In Vitro Activity of Essential Oils of Selected Plants against Salmonella enteritidis and Salmonella gallinarum of Poultry Origin 部分植物精油对家禽源肠炎沙门氏菌和鸡源沙门氏菌的体外活性及植物化学分析

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.110

Sidra Yasmin

{"title":"Phytochemical Analysis and In Vitro Activity of Essential Oils of Selected Plants against Salmonella enteritidis and Salmonella gallinarum of Poultry Origin","authors":"Sidra Yasmin","doi":"10.29261/pakvetj/2019.110","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.110","url":null,"abstract":"Received: Revised: Accepted: Published online: July 17, 2019 August 25, 2019 September 09, 2019 October 24, 2019 Antibiotic resistant Salmonella is a major threat to poultry industry and public health. Medicinal plants are an effective alternative of antibiotics for the control and treatment of multiple drug resistant Salmonella. The objective of this study was to evaluate the in-vitro activity of essential oils of some medicinal plants against multiple drug resistant Salmonella of poultry origin and to determine their active ingredients. Essential oils of Cuminum cyminum, Cinnamomum zeylanicum, Eucalyptus globulus, Allium sativum and Nigella sativa were prepared by steam distillation and their active ingredients were determined by Gas ChromatographyMass Spectrometry (GC-MS) analysis. Activity of oils against Salmonella enteritidis (n=05) and Salmonella gallinarum (n=05) was determined by well diffusion assay. Broth microdilution assay was employed to determine the minimum inhibitory concentrations of oils. Well diffusion assay revealed that C. zeylanicum and E. globulus had better activity against salmonellae (26±7.6 mm and 16±6.8 mm, respectively) as compared to C. cyminum, A. sativum and N. sativa (8±5.9, 10±6.1, 8±4.7 mm, respectively). Minimum inhibitory concentrations of C. zeylanicum and E. globulus against Salmonella were 64.1±32.1 and 68.9±32.9 μg/mL, respectively. The GC-MS analysis revealed presence of diverse phytochemicals in all essential oils. Major antimicrobial phyto-constituents of essential oils of E. globulus and C. zeylanicum were eucalyptol (82.85%) and 1R-α-Pinene (13.781%), and cinnamaldehyde (64.14%) and eugenol (8.9%), respectively. It is concluded that essential oils of C. zeylanicum and E. globulus have excellent in vitro antiSalmonella activity. It is insinuated that these extracts may be commercialized as an alternative of antibiotics for the control of Salmonellae in poultry after detailed in vivo evaluations. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41497501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa Kisspeptin受体的免疫细胞化学检测及其与水牛精子活力的关系

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2020.007

A. Hussain

{"title":"Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa","authors":"A. Hussain","doi":"10.29261/pakvetj/2020.007","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.007","url":null,"abstract":"Received: Revised: Accepted: Published online: November 11, 2019 December 11, 2019 December 15, 2019 January 21, 2020 Kisspeptin is a powerful regulator of the hypothalamic-pituitary-gonadal axis. It acts through its receptor GPR54 to regulate sexual maturation, oogenesis, spermatogenesis and fertilization. The present research was implemented to evaluate the presence of kisspeptin receptor on various regions of buffalo bull spermatozoa and to decipher its relationship with different motility parameters of the fresh spermatozoa. Standard swim-up protocol was performed on fresh ejaculates from Nili-Ravi buffalo bulls to obtain three hypothetical layers, having spermatozoa with enhanced motility and normal morphology. The progressive sperm motility was measured in each layer by using phase contrast microscopy. Methanol fixed sperm smears from each layer were processed for standard immunocytochemistry procedure for the detection of kisspeptin receptor using specific antibodies. Maximum GPR54 immunoreactivity was observed in the upper regions (head, neck/midpiece) and moderate immunoreactivity was seen in the lower region (tail) of the spermatozoa in all the three layers. No significant difference in total GPR54 expression was observed in the spermatozoa from three layers and no relationship was observed between percentage motility and GPR54 like ir. Present findings suggest that the buffalo sperm motility is not influenced by GPR54 expression on different regions of the sperm. However, robust GPR54 expression in dorsal areas of buffalo sperm, would raise possibility of a covert function of kisspeptin in the bubaline sperm biology. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44442120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of Yeast Addition to the Diet of Japanese Quails on Growth Performance, Selected Serum Parameters and Intestinal Morphology as well as Pathogens Reduction 日粮中添加酵母对日本鹌鹑生长性能、选定血清参数、肠道形态及致病菌减少的影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.125

A. A. El-Wahab, R. Mahmoud, Basma H. Marghani, Hossam Gadallah

{"title":"Effects of Yeast Addition to the Diet of Japanese Quails on Growth Performance, Selected Serum Parameters and Intestinal Morphology as well as Pathogens Reduction","authors":"A. A. El-Wahab, R. Mahmoud, Basma H. Marghani, Hossam Gadallah","doi":"10.29261/pakvetj/2019.125","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.125","url":null,"abstract":"Received: Revised: Accepted: Published online: May 08, 2019 November 03, 2019 November 04, 2019 December 26, 2019 The objectives of the current study were to investigate the impact of dietary yeast (Saccharomyces cerevisiae) supplementation on growth performance, some serum parameters, intestinal morphology and pathogens reduction in Japanese quails. In total, 300 d-old Japanese quails were randomly allocated to 5 dietary groups (6 replicates of 10 Japanese quails per pen). At beginning of the experiment (d 14), Japanese quails fed a basal diet without contain any yeast while, the other treatments provided basal diet plus 0.5, 1.5, 2.5 and 3.5% yeast on feed basis. The results showed that dietary supplementation of yeast, particularly at a level of inclusion of 3.5% on feed basis, resulted in highest BW for Japanese quails (P<0.05). Japanese quails fed diets supplemented with yeast reduced (P<0.05) cholesterol and triglycerides concentrations in serum compared to control. Dietary yeast supplementation at 3.5% reduced (P<0.05) interleukin 1 β (IL-1 β), IL-6 and TNF-α levels in serum compared to those fed dietary yeast at level of 0.5% or fed non supplemented diets. Feeding yeast at level of 3.5% led to increase in duodenal villus height significantly compared to other treatments. Japanese quails fed yeast supplemented diets at 2.5 or 3.5% reduced E. coli (P<0.05) and C. perfringens CFU counts in excreta than those fed non supplemented diet. It is concluded that dietary yeast supplementation of Japanese quails improved growth rate as a result of an increase in villus height and a reduce in the counts of E. coli and C. perfringens. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47403116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens 葡萄籽提取物对鸡胫骨软骨发育不良发生率、肝脏重量和胫骨血管生成的影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.109

Hammad Qamar

{"title":"Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens","authors":"Hammad Qamar","doi":"10.29261/pakvetj/2019.109","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.109","url":null,"abstract":"Received: Revised: Accepted: Published online: July 28, 2019 October 12, 2019 October 13, 2019 October 21, 2019 Among avian metabolic disorders, Tibial dyschondroplasia (TD) has a wide prevalence worldwide in chickens that are identified by non-mineralization and avascularization of the growth plate (GP). Grape seed extract (GSE) reduces the inflammation and oxidative stress by scavenging free oxygen specie and has some anti-cancer and angiogenic activity. This study was carried out to evaluate the role of GSE for the therapy of the chickens affected with TD. Arbor Acres broiler chicks (n=240) weighing 47±6g were fed starter feed till first 3 days and then portioned equally into three groups (Control, TD and GSE group; n=80 each). Thiram was incorporated in the feed of TD and GSE chicks until day 6 for inducing TD (50 mg/ kg of feed), whereas usual feed was offered to control group. All the birds in TD and GSE group showed the symptoms of TD on day 7 and GSE group was given GSE (50 mg/kg/day) intraperitoneally till the termination of the experiment. Chickens fed with thiram-feed exhibited high TD score and GP measurement. After the GSE treatment, the TD score dropped remarkably and, inflammation and TD lesions reduced. Moreover, body weight gain and liver weight were increased in comparison with TD chicks after application of GSE. GSE supplementation alleviated the adverse effects of thiram and increase in angiogenesis was observed. In conclusion, GSE recovered the TD affected broilers through downregulating the HIF-1α, Hsp90 and CA2 expressions. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45513306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Effect of Lactobacillus gallinarum PL 53 Supplementation on Xylose Absorption and Intestinal Morphology in Broilers Challenged with Campylobacter jejuni 添加鸡乳杆菌PL53对空肠弯曲杆菌攻击肉鸡木糖吸收和肠道形态的影响

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2020.011

Madiha Khan

{"title":"Effect of Lactobacillus gallinarum PL 53 Supplementation on Xylose Absorption and Intestinal Morphology in Broilers Challenged with Campylobacter jejuni","authors":"Madiha Khan","doi":"10.29261/pakvetj/2020.011","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.011","url":null,"abstract":"Received: Revised: Accepted: Published online: July 30, 2019 October 08, 2019 October 16, 2019 January 25, 2020 A feeding trial of indigenous probiotic lactobacilli strains including Lactobacillus gallinarum PL 53, L. paracasei PL 120 and L. gallinarum PL 149 was conducted to monitor the effect on intestinal absorption capacity and histological changes in broiler chicks challenged with Campylobacter jejuni. A total of 45, day old chicks were randomly assigned to nine experimental groups (5 birds/ group). Group A was negative and B positive control while group C, D and E prevention model, F, G and H treatment model and group I antibiotic control. Groups other than negative control received Campylobacter jejuni (10 CFUs/bird) challenge on day 14 by oral gavage. The groups of prevention model received lactobacilli (~10 CFUs/bird) strains from day 1-35 and treatment model received lactobacilli from day 15-35. The absorption capacity of intestine was monitored by concentration of D-xylose in plasma (0.5 and 1 hour post administration). The probiotic group L. gallinarum PL 53 (group C) significantly enhanced Dxylose absorption capacity as compared to control groups (59.69±2.07mg/dL) in both the prevention (72.83±1.20mg/dL) and treatment (71.11±2.27mg/dL) models. Intestinal morphology was observed by measuring villi length, width, crypt depth and surface area of the small intestine. PL 53 increased the villi length of ileum (930±4.00μm), jejunum (890±8.00μm) and duodenum (1350±4.00μm). The group which received L. gallinarum PL 53 strain had maximum surface area of the intestinal segments with 0.41±0.03mm, 0.45±0.03mm and 0.72±0.02mm of ileum, jejunum and duodenum, respectively. In conclusion, the PL 53 L. gallinarum may improve the gut performance and absorption capacity of broilers. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43545459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle 家牛巴贝虫分离种的分子特征及系统发育分析

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.003

Rimsha Farooq, M. Hafeez, M. Oneeb, A. Rafique, K. Ashraf, F. Aslam, Nauman Rauf, Kiran Khalid, Farrukh Bilal, S. Mahmood, A. Sattar

{"title":"Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle","authors":"Rimsha Farooq, M. Hafeez, M. Oneeb, A. Rafique, K. Ashraf, F. Aslam, Nauman Rauf, Kiran Khalid, Farrukh Bilal, S. Mahmood, A. Sattar","doi":"10.29261/pakvetj/2019.003","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.003","url":null,"abstract":"Received: Revised: Accepted: Published online: June 15, 2019 September 25, 2019 January 06, 2020 January 07, 2020 Babesiosis is a key tick born disease caused by the protozoal parasites belonging to the genus Babesia, cosmopolitan in nature and infecting enormous range of large ruminants. Study was intended to determine the presence of local isolates of Babesia species (B. bovis, B. bigemina) their molecular characterization and phylogenetic relationship in cattle (district Narowal) Punjab. Blood samples were collected from 200 suspected cattle, from seven villages of selected district. DNA was extracted by using DNA zole, Gene-all Kit, manual method and subjected to PCR for amplification using specific RLB and Universal Primers. Quantification of protozoan parasite was performed by RT PCR using Solis biodine kit. A total of 36 out of 200 animal samples, were found positive by microscopy. PCR positive samples (17) were amplified and bands of strength 520-bp and 800-bp with specific RLB and for universal primers respectively were obtained for Babesia species. These 17 samples were sent for sequencing and by using Clustal W; Bioedit software with Neighbor-joining method phylogenetic tree was established. Current study reported the presence of B. bigemina i.e. 18% (conventional method) in large ruminants of district Narowal, Pakistan, and confirmation by (molecular characterization) PCR (8.5%) and phylogenetic relationship. Phylogenetic results showed that our study has homology with Babesia bigemina strain. ©2020 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43648965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Postpartum Cyclicity of Holstein-Friesian Crossbred Cows Shows Relation with Serum Biochemical Profiles during 45-60 Days Postpartum 荷母杂交奶牛产后周期与产后45 ~ 60 d血清生化指标的关系

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.122

A. Uddin, M. Atikuzzaman, M. S. Islam, M. Hossain

{"title":"Postpartum Cyclicity of Holstein-Friesian Crossbred Cows Shows Relation with Serum Biochemical Profiles during 45-60 Days Postpartum","authors":"A. Uddin, M. Atikuzzaman, M. S. Islam, M. Hossain","doi":"10.29261/pakvetj/2019.122","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.122","url":null,"abstract":"Received: Revised: Accepted: Published online: September 23, 2019 November 20, 2019 November 22, 2019 December 06, 2019 Reproductive performance of dairy cows is greatly affected by energy, protein and mineral profiles, which is also connected to postpartum anestrus. The present study was conducted during the period from December 2018 to May 2019 on 30 local x Holstein-Friesian crossbred lactating cows with parity 1-5 and body condition score of 2.0-3.5. This study aimed to compare serum glucose, cholesterol, triglycerides, total proteins, urea nitrogen, calcium, phosphorus and magnesium concentrations between anestrus and normal cyclic crossbred cows during 45 to 60 days postpartum. Automated biochemical analyzer was employed to measure these concentrations, using 15 cows in each group. The serum of postpartum anestrus cows contained significantly lower (P<0.05) concentrations of glucose, total proteins, calcium and magnesium compared to normal cyclic cows, while urea nitrogen concentration was significantly increased (P<0.05) in the postpartum anestrus cows. However, concentrations of other serum biochemical metabolites did not differ between cows of the two groups. These results suggest that blood glucose, total proteins, calcium, magnesium and urea nitrogen might have an effect on the cyclicity of Holstein-Friesian crossbred cows during 45 to 60 days postpartum. However, further studies are recommended to explore the underlying mechanism for the role of these metabolites in the cyclicity of postpartum cows. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42563055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Casticin Alleviates Testicular and Spermatological Damage Induced by Cisplatin in Rats 蓖麻素减轻顺铂致大鼠睾丸和精子损伤

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.127

M. Ijaz

{"title":"Casticin Alleviates Testicular and Spermatological Damage Induced by Cisplatin in Rats","authors":"M. Ijaz","doi":"10.29261/pakvetj/2019.127","DOIUrl":"https://doi.org/10.29261/pakvetj/2019.127","url":null,"abstract":"Received: Revised: Accepted: Published online: September 11, 2019 September 19, 2019 December 02, 2019 December 31, 2019 Cancer is one of the leading causes of mortality in animals. Cisplatin (CP) is a chemotherapeutic drug, which is used in treatment of several types of solid tumors. However, it causes numerous side effects on multiple organs, specifically to testicular tissues. This study elucidated the alleviative effects of casticin (CAS) on CP induced damages in testes of rats. Twenty-four adult male Sprague Dawley rats were used and divided into four groups. Each group consisted of six animals. These four groups were treated as followed: Control group (0.9% saline), CP treated group (10 mg/kg. b.wt.), CP+CAS treated group (10 mg/kg. b.wt. CP and 50 mg/kg. b.wt. CAS) and CAS treated group (50 mg/kg. b.wt.). The treatment duration was one week. Biochemical, histomorphometric, hormonal and spermatogenic profiles were assessed. Results of present investigation indicated that CP treatment significantly decreased the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and glutathione reductase (GSR), while thiobarbituric acid reactive substances (TBARS) level was increased. Furthermore, the count of germ cells and Leydig cells, epithelial height and diameter of seminiferous tubules, plasma and intratesticular testosterone concentrations as well as daily sperm production (DSP) along with its efficiency were decreased. Whereas, luminal diameter and interstitial spaces were increased. CAS is a flavonoid, which exhibits antioxidant and reactive oxygen species (ROS) scavenging properties. Therefore, it restored these damages to some degree by attenuating CP induced toxicity in testicular tissues. ©2019 PVJ. All rights reserved","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45657759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Autosomal Cholesterol Deficiency in a Holstein Calf 荷斯坦牛常染色体胆固醇缺乏症

IF 2.3 3区农林科学

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.120

J. Jacinto, M. Bolcato, Drögemüller Cord, A. Gentile, G. Militerno

引用次数: 1