Dose and Time-Related Pathological and Genotoxic Studies on Thiamethoxam in Fresh Water Fish (Labeo rohita) in Pakistan

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI:10.29261/pakvetj/2020.002

A. Ghaffar

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引用次数: 30

Abstract

Received: Revised: Accepted: Published online: July 17, 2019 December 01, 2019 December 02, 2019 January 07, 2020 A total of 60 fresh-water fish were obtained from a local fish breeding center and carefully transported in plastic bags having adequate amount of oxygen and water. After 7 days of acclimatization, all the fish were randomly divided and kept in five equal groups (A-E). The experimental fish were exposed to various concentrations (0, 0.5, 1.0, 1.5 and 2.0 mg/L) of thiamethoxam for a period of 120 h. Blood and other tissue samples of each treated fish were collected after 72, 96 and 120 h. Various physical responses like operculum and bouncing movement, mucus secretion, darkening of fins, fin tremors, swimming in isolation on one side, surface breathing and erratic swimming were observed in fish treated with higher concentrations. Results indicated that the values of red blood cell counts (RBCs), packed cell volume (PCV) and hemoglobin (Hb) concentrations were significantly lower and white blood cell (WBCs)and neutrophil counts increased significantly in thiamethoxam treated fish as compared to unexposed fish. The frequency of various nuclear (micronuclei, erythrocytes with nuclear remnant, erythrocytes with condensed nuclei and erythrocytes without nucleus) and morphological changes (leptocytes, stomatocytes, dividing erythrocytes and tear shape erythrocyte) intreated fish was significantly higher as compared to control group. Microscopic analysis of gills tissues of various experimental fish exhibited atrophy of secondary lamellae, pyknosis of lamellar epithelial pillar cells, lamellar degeneration, congestion, aneurysm and curling of lamellae. ©2019 PVJ. All rights reserved

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噻虫嗪对巴基斯坦淡水鱼(Labeo rohita)的剂量和时间相关病理和基因毒性研究

收到：修订：接受：在线发布：2019年7月17日2019年12月1日2019年2月2日2020年1月7日从当地鱼类养殖中心获得了总共60条淡水鱼，并用装有足够氧气和水的塑料袋小心运输。驯化7天后，将所有鱼类随机分为5组（A-E）。实验鱼暴露于不同浓度（0、0.5、1.0、1.5和2.0 mg/L）的噻虫嗪达120小时。在72、96和120小时后收集每只处理过的鱼的血液和其他组织样本，在高浓度处理的鱼身上观察到表面呼吸和不稳定的游动。结果表明，与未暴露的鱼相比，噻虫嗪处理的鱼的红细胞计数（RBCs）、堆积细胞体积（PCV）和血红蛋白（Hb）浓度值显著降低，白细胞（WBCs）和中性粒细胞计数显著增加。与对照组相比，鱼的各种细胞核（微核、有核残留的红细胞、有核浓缩的红细胞和无核红细胞）和形态变化（钩端细胞、口腔细胞、分裂红细胞和泪痕状红细胞）的频率显著较高。对各种实验鱼鳃组织的显微镜分析显示，次级片层萎缩、片层上皮柱细胞固缩、片层变性、充血、动脉瘤和片层卷曲。©2019 PVJ。保留所有权利

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.