slc11a1 (Nramp1)基因与巴基斯坦不同品种牛和水牛对布鲁氏菌病的易感和抗性比较

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES

Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI:10.29261/pakvetj/2019.091

R. Akhtar

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引用次数: 5

摘要

接收：修订：接受：在线发布：2019年2月19日2019年6月20日2019年7月3日2019年8月15日据观察，不同的Nramp1基因型与布鲁氏菌耐药性或易感性有关。因此，本研究旨在以Nramp1基因型为基础，了解巴基斯坦本地和外来品种对布鲁氏菌的抗性和易感性。对两个品种的水牛和五个品种的牛进行了布鲁氏菌病筛查。RBPT测定牛和水牛的总血清阳性率分别为77.5%和33.5%。PCR结果显示牛和水牛布鲁氏菌阳性率分别为54.5%和25%。与Kundi品种相比，Nili Ravi水牛属于抗性基因型Nramp1 BB。与杂交品种和Friesian相比，Sahiwal品种对布鲁氏菌病的抗性更强。系统发育分析表明，本地品种Nramp1基因与中国牛牛亲缘关系更为密切。这种基因抗性可用于动物的基因组选择，以减少布鲁氏菌病造成的疾病成本和经济损失。©2019 PVJ。保留所有权利

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Comparative Association of slc11a1 (Nramp1) Gene with Susceptibility and Resistance to Brucellosis in Various Cattle and Buffaloes Breeds of Pakistan

Received: Revised: Accepted: Published online: February 19, 2019 June 20, 2019 July 03, 2019 August 15, 2019 It is observed that different Nramp1 genotypes are linked with Brucella resistance or susceptibility. So, current study aimed to find the Brucella resistance and susceptibility of local and exotic breeds of Pakistan on the basis of Nramp1 genotypes. Two breeds of buffaloes and five breeds of cattle were screened for brucellosis. The overall seropositivity determined by RBPT in cattle and buffaloes was 77.5% and 33.5% respectively. PCR results showed 54.5 and 25% brucella positive cases in cattle and buffaloes respectively. Nili-Ravi buffaloes pertain resistant genotype Nramp1 BB as compared to Kundi breeds of buffaloes possessed susceptible genotype Nramp1 AA. Similarly, Sahiwal breed was more resistant for brucellosis compared to cross breeds and Friesian. Phylogenetic analysis showed that Nramp1 gene of local breed had more closeness with B. taurus of China. This gene resistance can be used for genomic selection of animals to reduce disease cost and economic losses due to brucellosis. ©2019 PVJ. All rights reserved

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.