Pathogens and disease最新文献_第8页

Three-dimensional models of the cervicovaginal epithelia to study host-microbiome interactions and sexually transmitted infections. 三维模型的宫颈阴道上皮研究宿主-微生物相互作用和性传播感染。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-27 DOI: 10.1093/femspd/ftac026

Vonetta L Edwards, Elias McComb, Jason P Gleghorn, Larry Forney, Patrik M Bavoil, Jacques Ravel

{"title":"Three-dimensional models of the cervicovaginal epithelia to study host-microbiome interactions and sexually transmitted infections.","authors":"Vonetta L Edwards, Elias McComb, Jason P Gleghorn, Larry Forney, Patrik M Bavoil, Jacques Ravel","doi":"10.1093/femspd/ftac026","DOIUrl":"https://doi.org/10.1093/femspd/ftac026","url":null,"abstract":"2D cell culture systems have historically provided controlled, reproducible means to analyze host-pathogen interactions observed in the human reproductive tract. Although inexpensive, straightforward, and requiring a very short time commitment, these models recapitulate neither the functionality of multilayered cell types nor the associated microbiome that occurs in a human. Animal models have commonly been used to recreate the complexity of human infections. However, extensive modifications of animal models are required to recreate interactions that resemble those in the human reproductive tract. 3D cell culture models have emerged as alternative means of reproducing vital elements of human infections at a fraction of the cost of animal models and on a scale that allows for replicative experiments. Here, we describe a new 3D model that utilizes transwells with epithelial cells seeded apically and a basolateral extracellular matrix (ECM)-like layer. The model produced tissues with morphologic and physiological resemblance to human cervical and vaginal epithelia, including mucus levels produced by cervical cells. Infection by Chlamydia trachomatis and Neisseria gonorrhoeae was demonstrated, as well as the growth of bacterial species observed in the human vaginal microbiota. This enabled controlled mechanistic analyses of the interactions between host cells, the vaginal microbiota, and STI pathogens. Affordable and semi high-throughput 3D models of the cervicovaginal epithelia that are physiologically relevant by sustaining vaginal bacterial colonization, and facilitate studies of chlamydial and gonococcal infections.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10603496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examination of vertical transmission of Bartonella quintana in body lice following multiple infectious blood meals. 多次感染性血食后体虱垂直传播的检验。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-17 DOI: 10.1093/femspd/ftac028

Lauren Kress, Rashaun Potts, Jose E Pietri

{"title":"Examination of vertical transmission of Bartonella quintana in body lice following multiple infectious blood meals.","authors":"Lauren Kress, Rashaun Potts, Jose E Pietri","doi":"10.1093/femspd/ftac028","DOIUrl":"https://doi.org/10.1093/femspd/ftac028","url":null,"abstract":"Bartonella quintana is a re-emerging louse-borne pathogen. Horizontal transmission from the body louse vector (Pediculus humanus humanus) to a human host occurs through contact with infectious louse feces containing a high concentration of the bacteria. However, questions have remained about whether vertical transmission from infected vectors to their progeny, which could significantly influence the dynamics of transmission to humans, occurs in body lice. To address this subject, we performed a series of controlled laboratory experiments that examined the presence of B. quintana on the surface of and within eggs produced by female body lice that were provisioned multiple infectious blood meals to recapitulate the natural pathogen acquisition process. Our results demonstrate that B. quintana DNA can be detected from the surface of eggs by qPCR due to vertical transfer of infectious feces to the egg sheath during or after oviposition. However, viable B. quintana could not be cultured from the hemolymph of adult female lice or from within eggs that were surface sterilized, indicating a lack of true transovarial transmission. Based on this evidence, vertical transfer of B. quintana from infected adult lice to their eggs probably has a limited impact on the dynamics of transmission to humans.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9384838/pdf/ftac028.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10119636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Using champion-oriented mindset to overcome the challenges of graduate school: impact of workshop for graduate school skills on underrepresented minority retention. 用冠军导向的心态克服研究生院的挑战:研究生院技能研讨会对未被充分代表的少数民族保留的影响。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-13 DOI: 10.1093/femspd/ftac024

Andrea G Marshall, Caroline B Palavicino-Maggio, Kit Neikirk, Zer Vue, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton

{"title":"Using champion-oriented mindset to overcome the challenges of graduate school: impact of workshop for graduate school skills on underrepresented minority retention.","authors":"Andrea G Marshall, Caroline B Palavicino-Maggio, Kit Neikirk, Zer Vue, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton","doi":"10.1093/femspd/ftac024","DOIUrl":"https://doi.org/10.1093/femspd/ftac024","url":null,"abstract":"Despite efforts to increase diversity, a glaring underrepresentation of minorities (URM) persists in the fields of science, technology, engineering, and mathematics (STEM). Graduate school can be a stressful step in the STEM pipeline, especially for students previously unaware of the structure and challenges of postgraduate education. To promote successful minority participation in STEM and prepare prospective students for the impending challenges of applying for and attending graduate school, we developed a workshop based on the mentoring and fostering of a champion-oriented mindset entitled, \"The Trials and Tribulations of Graduate School: How Do You Make an Impact?.\" Students from the HBCU Winston-Salem State University attended the workshop, and a pre/post-a 10-point Likert scale-based survey was administered. The questions used in this seminar were newly designed by the authors as program evaluations. The results suggest that the workshop was well-received by the students and provided information that they considered helpful to help navigate the graduate school process.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375733/pdf/ftac024.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10078825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The role of mentoring in promoting diversity equity and inclusion in STEM Education and Research. 指导在促进STEM教育和研究中的多样性公平和包容方面的作用。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-07-21 DOI: 10.1093/femspd/ftac019

Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton

{"title":"The role of mentoring in promoting diversity equity and inclusion in STEM Education and Research.","authors":"Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton","doi":"10.1093/femspd/ftac019","DOIUrl":"https://doi.org/10.1093/femspd/ftac019","url":null,"abstract":"The success of mentoring derives from active and respectful listening and the willingness to learn and accept opportunities for personal growth. This shapes every trainee and their destined path in science, technology, engineering, and mathematics (STEM). The act of cultivating rapport, asking, and pondering meaningful questions, and receiving constructive feedback are critical to support a productive mentoring relationship. Successful mentoring in STEM can be established and allow mentees, especially underrepresented minorities (URMs), to flourish in an environment where they feel welcomed and supported. However, mentees from underrepresented groups often experience inadequate mentoring due to a mentor's lack of awareness, poor trainings themselves, or lack of understanding of the mentee's hardships. It is important for mentors and mentees to work together to promote diversity, equity, and inclusion (DEI) in STEM education through creativity, authenticity, and networking. We analyzed data obtained from students who attended a recent workshop that are interested in going to graduate school. Our results show that despite low initial expectations for the workshop, many students were satisfied in the knowledge they gleaned. The future and role of diversity in STEM within these underrepresented groups lies in community support and an important role that they can play in the lives of others through DEI initiatives and throughout their careers all of which involves positive mentoring.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302695/pdf/ftac019.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9641350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An effective workshop on "How to be an Effective Mentor for Underrepresented STEM Trainees". 一个关于“如何成为STEM受训者的有效导师”的有效研讨会。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-07-06 DOI: 10.1093/femspd/ftac022

Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton

{"title":"An effective workshop on \"How to be an Effective Mentor for Underrepresented STEM Trainees\".","authors":"Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton","doi":"10.1093/femspd/ftac022","DOIUrl":"https://doi.org/10.1093/femspd/ftac022","url":null,"abstract":"Despite an increase in programming to promote persons excluded by their ethnicity or race (PEER) scholars, minorities remain underrepresented in many STEM programs. The academic pipeline is largely leaky for underrepresented minority (URM) scholars due to a lack of effective mentorship. Many URM students experience microaggressions and discrimination from their mentors due to a lack of quality mentorship training. In this workshop, we provide a framework to show trainees what effective mentoring looks like. Mentees, especially URM trainees, can flourish in effective mentoring environments where they feel welcomed and can comfortably develop new ideas without feeling threatened by external factors. Effective mentoring environments provide motivational support, empathy, cultural competency, and training. This workshop explains facets of effective mentoring to students, as well as highlights to URM trainees why mentors can serve as valuable resources.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258687/pdf/ftac022.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9633385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of concern on: Mitogen-activated protein kinases regulate Mycobacterium avium-induced tumor necrosis factor-α release from macrophages. 对丝裂原活化蛋白激酶调控巨噬细胞释放瘤坏死因子α的关注

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-06-22 DOI: 10.1093/femspd/ftac017

引用次数: 7

Coexistence of blaNDM-1, blaOXA-51, blaOXA-23 and armA in conjunction with novel mutations detected in RND efflux pump regulators in tigecycline resistant clinical isolates of Acinetobacter baumannii. 在对替加环素耐药的鲍曼不动杆菌临床分离株中检测到blaNDM-1、blaOXA-51、blaOXA-23和armA的共存以及RND外排泵调节因子的新突变。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-06-15 DOI: 10.1093/femspd/ftac020

A. Sawant, Sudhakar Pagal, Ashutosh Kumar Amar, L. Panda, S. Devi C., P. Shashikala, R. Kanungo, K. Prashanth

{"title":"Coexistence of blaNDM-1, blaOXA-51, blaOXA-23 and armA in conjunction with novel mutations detected in RND efflux pump regulators in tigecycline resistant clinical isolates of Acinetobacter baumannii.","authors":"A. Sawant, Sudhakar Pagal, Ashutosh Kumar Amar, L. Panda, S. Devi C., P. Shashikala, R. Kanungo, K. Prashanth","doi":"10.1093/femspd/ftac020","DOIUrl":"https://doi.org/10.1093/femspd/ftac020","url":null,"abstract":"This study has investigated a total of 51 A. baumannii isolates for the prevalence of resistant determinants in tigecycline susceptible and non-susceptible clinical isolates of A. baumannii. Antimicrobial susceptibility testing revealed 74% of isolates were tigecycline resistant. Mutations in RND-efflux pump regulatory genes and the expression of efflux pump genes were measured in tigecycline resistant isolates. There was a strong co-relation between the blaNDM-1 and armA wherein majority of the isolates that are positive for blaNDM-1 have also harbored armA. Compared with TSAB (tigecycline susceptible A. baumannii), TNAB (tigecycline non-susceptible A. baumannii) isolates show increased distribution of blaNDM-1 (p = 0.048), blaIMP-1 (p<0.0001) and blaOXA-51 (p = 0.0029) carbapenemase genes. The variants of RND-efflux pump regulatory genes due to amino-acid mutations in adeS (F12S, K84E, W61R, N268H and Q299R) and adeL (G21R and Q262R) were identified in tigecycline resistant isolates as well as ISAba1 mediated disruption of adeN were observed causing overexpression of adeIJK efflux pump. Additionally, mutations in adeRS were also associated with increased expression of adeABC efflux pump. Besides, TNAB isolates showed significantly (p<0.0001) higher ability of biofilm formation as compared to TSAB isolates. The tigecycline resistance due to mutations in contemporary A. baumannii isolates having a higher ability to form biofilm may pose therapeutic difficulties.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48021488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunopotentiation by linking Hsp70 T-cell epitopes to Gag-Pol-Env-Nef-Rev multiepitope construct and increased IFN-gamma secretion in infected lymphocytes. 通过将Hsp70 T细胞表位连接到Gag-Pol-Ev-Nef-Rev多表位构建体来增强免疫，并增加感染淋巴细胞中的IFN-γ分泌。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-06-15 DOI: 10.1093/femspd/ftac021

E. Akbari, S. Ajdary, E. M. Ardakani, Elnaz Agi, A. Milani, Masoud Seyedinkhorasani, V. Khalaj, A. Bolhassani

{"title":"Immunopotentiation by linking Hsp70 T-cell epitopes to Gag-Pol-Env-Nef-Rev multiepitope construct and increased IFN-gamma secretion in infected lymphocytes.","authors":"E. Akbari, S. Ajdary, E. M. Ardakani, Elnaz Agi, A. Milani, Masoud Seyedinkhorasani, V. Khalaj, A. Bolhassani","doi":"10.1093/femspd/ftac021","DOIUrl":"https://doi.org/10.1093/femspd/ftac021","url":null,"abstract":"Therapeutic human immunodeficiency virus (HIV) vaccines can boost the anti-HIV host immunity to control viral replication and eliminate viral reservoirs in the absence of anti-retroviral therapy. In this study, two computationally designed multiepitope Gag-Pol-Env-Nef-Rev and Hsp70-Gag-Pol-Env-Nef-Rev constructs harboring immunogenic and highly conserved HIV T cell epitopes were generated in E. coli as polypeptide vaccine candidates. Furthermore, the multiepitope gag-pol-env-nef-rev and hsp70-gag-pol-env-nef-rev DNA vaccine constructs were prepared and complexed with MPG cell-penetrating peptide. The immunogenicity of the multiepitope constructs were evaluated using the homologous and heterologous prime/boost strategies in mice. Moreover, the secretion of IFN-γ was assessed in infected lymphocytes in vitro. Our data showed that the homologous polypeptide regimens could significantly induce a mixture of IgG1 and IgG2a antibody responses, activate T cells to secret IFN-γ, IL-5, IL-10, and generate Granzyme B. Moreover, IFN-γ secretion was significantly enhanced in single-cycle replicable (SCR) HIV-1 virions-infected splenocytes in these groups compared to uninfected splenocytes. The linkage of heat shock protein 70 (Hsp70) epitopes to Gag-Pol-Env-Nef-Rev polypeptide in the homologous regimen increased significantly cytokines and Granzyme B levels, and IFN-γ secretion in virions-infected splenocytes. Briefly, both designed constructs in the homologous regimens can be used as a promising vaccine candidate against HIV infection.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47088597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

P. falciparum PfRUVBL proteins binds at TARE region and var gene promoter located in subtelomeric region. 恶性疟原虫PfRUVBL蛋白结合于TARE区，var基因启动子位于亚端粒区。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-05-31 DOI: 10.1093/femspd/ftac018

H. Saxena, Ashish Gupta

{"title":"P. falciparum PfRUVBL proteins binds at TARE region and var gene promoter located in subtelomeric region.","authors":"H. Saxena, Ashish Gupta","doi":"10.1093/femspd/ftac018","DOIUrl":"https://doi.org/10.1093/femspd/ftac018","url":null,"abstract":"In order to survive and establish infection, Plasmodium parasite employ various strategies to evade host immune response. Var genes family, a repertoire of 60 genes, express parasite-specific protein PfEMP1, a variable surface antigen, on the membrane of infected erythrocyte, and by continuously switching the variants of PfEMP1, helps the parasite to avoid detection and destruction by host immune system during intra-erythrocytic developmental cycle. Although chromatin modifications are recognized to be a prominent phenomenon in regulation of mono-allelic expression of these var genes, however the precise histone codes and molecular players & mechanisms guiding these modifications are yet to be unravelled in depth. In this study, we have functionally characterized RUVBL proteins of P. falciparum and shown that PfMYST (an essential lysine acetyl transferase) and PfRUVBL protein complex occupy the TARE region and var gene promoter in ring stage of the parasite. Further we have demonstrated that PfMYST/PfRUVBL complex interact with core histone, H3 & H4. Overall the findings of this study adds a layer by identifying the potential role of epigenetic regulators, PfMYST & PfRUVBL in regulation of monoallelic expression of var genes in malaria parasite.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43094655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

AZI2 positively regulates the induction of type I interferon in influenza-trigger pediatric pneumonia. AZI2正调控I型干扰素在流感引发的儿童肺炎中的诱导作用。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-05-20 DOI: 10.1093/femspd/ftac016

Meili Wei, Ya-nan Zheng, Jing Xu, Qiwei Sun

{"title":"AZI2 positively regulates the induction of type I interferon in influenza-trigger pediatric pneumonia.","authors":"Meili Wei, Ya-nan Zheng, Jing Xu, Qiwei Sun","doi":"10.1093/femspd/ftac016","DOIUrl":"https://doi.org/10.1093/femspd/ftac016","url":null,"abstract":"5-azacytidine-induced protein 2 (AZI2) is known to have a crucial role in antiviral innate immunity. This study aims to explore the roles of AZI2 in influenza-trigger pediatric pneumonia and its molecular mechanism. qPCR and immunoblotting assays were used to determine the levels of target genes and proteins. The lung infection mouse model was established by using PR8 H1N1 virus in AZI2 germline knockout (AZI2-/-) and wide-type (WT) mice. In addition, HEK293T cell-based luciferase reporter assays were used to investigate the regulatory effects of AZI2 on type I interferon. Immune precipitation and immunofluorescence staining were used to evaluate the interactions between AZI2 and TANK binding kinase 1 (TBK1). We observed an elevation in the expressions of IFN-I and AZI2 in peripheral blood mononuclear cells from the pneumonia patients with mild symptoms. Interestingly, AZI2 deficiency deteriorated the influenza-induced pathological symptoms in the lung as well as reduced the survival rate. It was further showed that AZI2 positively regulated the expressions of type I interferon, inflammatory cytokines, and IFN production-related genes. The molecular mechanism data revealed that AZI2 regulated the interactions between TBK1 and TANK. In summary, AZI2 positively regulates type I interferon production in influenza-induced pediatric pneumonia by promoting the interactions between TBK1 and TANK.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48079282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2