Pathogens and disease最新文献_第10页

3-indoleacetonitrile attenuates biofilm formation and enhances sensitivity to imipenem in Acinetobacter baumannii. 3-吲哚乙腈减弱鲍曼不动杆菌生物膜的形成并增强对亚胺培南的敏感性。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-17 DOI: 10.1093/femspd/ftac029

Shruti Kashyap, Harsimran Sidhu, Prince Sharma, Neena Capalash

{"title":"3-indoleacetonitrile attenuates biofilm formation and enhances sensitivity to imipenem in Acinetobacter baumannii.","authors":"Shruti Kashyap, Harsimran Sidhu, Prince Sharma, Neena Capalash","doi":"10.1093/femspd/ftac029","DOIUrl":"https://doi.org/10.1093/femspd/ftac029","url":null,"abstract":"Acinetobacter baumannii poses a global danger due to its ability to resist most of the currently available antimicrobial agents. Furthermore, the rise of carbapenem-resistant A. baumannii isolates has limited the treatment options available. In the present study, plant auxin 3-indoleacetonitrile (3IAN) was found to inhibit biofilm formation and motility of A. baumannii at sublethal concentration. Mechanistically, 3IAN inhibited the synthesis of the quorum sensing signal 3-OH-C12-HSL by downregulating the expression of the abaI autoinducer synthase gene. 3IAN was found to reduce the minimum inhibitory concentration of A. baumannii ATCC 17978 against imipenem, ofloxacin, ciprofloxacin, tobramycin, and levofloxacin, and significantly decreased persistence against imipenem. Inhibition of efflux pumps by downregulating genes expression may be responsible for enhanced sensitivity and low persistence. 3IAN reduced the resistance to imipenem in carbapenem-resistant A. baumannii isolates by downregulating the expression of OXA β-lactamases (blaoxa-51 and blaoxa-23), outer membrane protein carO, and transporter protein adeB. These findings demonstrate the therapeutic potential of 3IAN, which could be explored as an adjuvant with antibiotics for controlling A. baumannii infections.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40615462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Tumor necrosis factor-α-inducing protein of Helicobacter pylori promotes epithelial-mesenchymal transition and cancer stem-like cells properties via activation of Wnt/β-catenin signaling pathway in gastric cancer cells. 幽门螺杆菌肿瘤坏死因子-α-诱导蛋白通过激活胃癌细胞Wnt/β-catenin信号通路促进上皮-间质转化和癌干细胞样细胞特性。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-17 DOI: 10.1093/femspd/ftac025

Kaiyun Guo, Jie Duan, Jingwen Lu, Lingqiao Xiao, Liang Han, Shasha Zeng, Xin Tang, Wenjing Li, Lijun Huang, Yan Zhang

{"title":"Tumor necrosis factor-α-inducing protein of Helicobacter pylori promotes epithelial-mesenchymal transition and cancer stem-like cells properties via activation of Wnt/β-catenin signaling pathway in gastric cancer cells.","authors":"Kaiyun Guo, Jie Duan, Jingwen Lu, Lingqiao Xiao, Liang Han, Shasha Zeng, Xin Tang, Wenjing Li, Lijun Huang, Yan Zhang","doi":"10.1093/femspd/ftac025","DOIUrl":"https://doi.org/10.1093/femspd/ftac025","url":null,"abstract":"Tumor necrosis factor-α-inducing protein (Tipα) is a newly identified toxin that promotes the inflammation and carcinogenesis caused by Helicobacter pylori. However, its mechanism of pathogenesis is still unclear. To investigate the carcinogenic mechanisms of Tipα, SGC7901 cells and SGC7901-derived cancer stem-like cells (CSCs) were stimulated by recombinant Tipα with or without Wnt/β-catenin signaling inhibitor XAV939. qRT-PCR and Western blotting were employed to detect expression of epithelial-mesenchymal transition (EMT), CSCs markers and downstream target genes of this signaling pathway. The cell migration ability was measured by wound healing assay and transwell assay. Our results indicated that Tipα promoted CSC properties of SGC7901 spheroids, including increased expression of CSC specific surface markers CD44, Oct4 and Nanog and an increased capacity for self-renewal. Tipα activated Wnt/β-catenin signaling in both SGC7901 cells or CSCs. Furthermore, Tipα induced the EMT and increased the expressions of downstream target genes of this signaling, including c-myc, cyclin D1 and CD44. However, XAV939 pretreatment inhibited Tipα-induced EMT and CSC properties in SGC7901 cells or CSCs. These results suggest that Tipα promotes EMT and CSC-like properties in gastric cancer cells through activation of Wnt/β-catenin signaling pathway, thereby accelerating the progression of gastric cancer.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40463487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Examination of vertical transmission of Bartonella quintana in body lice following multiple infectious blood meals. 多次感染性血食后体虱垂直传播的检验。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-17 DOI: 10.1093/femspd/ftac028

Lauren Kress, Rashaun Potts, Jose E Pietri

{"title":"Examination of vertical transmission of Bartonella quintana in body lice following multiple infectious blood meals.","authors":"Lauren Kress, Rashaun Potts, Jose E Pietri","doi":"10.1093/femspd/ftac028","DOIUrl":"https://doi.org/10.1093/femspd/ftac028","url":null,"abstract":"Bartonella quintana is a re-emerging louse-borne pathogen. Horizontal transmission from the body louse vector (Pediculus humanus humanus) to a human host occurs through contact with infectious louse feces containing a high concentration of the bacteria. However, questions have remained about whether vertical transmission from infected vectors to their progeny, which could significantly influence the dynamics of transmission to humans, occurs in body lice. To address this subject, we performed a series of controlled laboratory experiments that examined the presence of B. quintana on the surface of and within eggs produced by female body lice that were provisioned multiple infectious blood meals to recapitulate the natural pathogen acquisition process. Our results demonstrate that B. quintana DNA can be detected from the surface of eggs by qPCR due to vertical transfer of infectious feces to the egg sheath during or after oviposition. However, viable B. quintana could not be cultured from the hemolymph of adult female lice or from within eggs that were surface sterilized, indicating a lack of true transovarial transmission. Based on this evidence, vertical transfer of B. quintana from infected adult lice to their eggs probably has a limited impact on the dynamics of transmission to humans.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9384838/pdf/ftac028.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10119636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impact on quinolone resistance of plasmid-mediated quinolone resistance gene and mutations in quinolone resistance-determining regions in extended spectrum beta lactamase-producing Klebsiella pneumoniae isolated from urinary tract infection patients. 质粒介导的喹诺酮类耐药基因对尿路感染患者产β -内酰胺酶肺炎克雷伯菌喹诺酮类耐药区突变的影响

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-17 DOI: 10.1093/femspd/ftac030

Reo Onishi, Katsumi Shigemura, Kayo Osawa, Young-Min Yang, Koki Maeda, Hiroshi Tanimoto, Mitsuki Kado, Shiuh-Bin Fang, Shian-Ying Sung, Takayuki Miyara, Masato Fujisawa

{"title":"Impact on quinolone resistance of plasmid-mediated quinolone resistance gene and mutations in quinolone resistance-determining regions in extended spectrum beta lactamase-producing Klebsiella pneumoniae isolated from urinary tract infection patients.","authors":"Reo Onishi, Katsumi Shigemura, Kayo Osawa, Young-Min Yang, Koki Maeda, Hiroshi Tanimoto, Mitsuki Kado, Shiuh-Bin Fang, Shian-Ying Sung, Takayuki Miyara, Masato Fujisawa","doi":"10.1093/femspd/ftac030","DOIUrl":"https://doi.org/10.1093/femspd/ftac030","url":null,"abstract":"Klebsiella pneumoniae is a typical pathogen in urinary tract infections (UTI), and the emergence of extended spectrum beta-lactamase (ESBL)-producing strains has been frequently reported, accompanied by higher quinolone resistance rates. There are two major mechanisms of quinolone resistance, mutations in quinolone resistance-determining regions (QRDR) and the presence of the plasmid-mediated quinolone resistance (PMQR) genes. This study aimed to investigate quinolone resistance among 105 ESBL-producing K. pneumoniae specimens isolated from UTI patients in Indonesia. These were characterized for antimicrobial resistance to nalidixic acid, ciprofloxacin, and levofloxacin, QRDR mutations in gyrA and parC and the presence of PMQR genes. We found that 84.8% of the collected isolates were resistant to at least one of the quinolones. QRDR mutation in gyrA was observed in 49.5% of these strains and parC mutations in 61.0%. PMQR genes were identified in 84.8% of strains. The QRDR mutations clearly had a greater effect on resistance than the PMQR genes. In conclusion, we found high quinolone resistance rates in Indonesian ESBL-producing K. pneumoniae, in which QRDR mutation played a major role.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40647830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Using champion-oriented mindset to overcome the challenges of graduate school: impact of workshop for graduate school skills on underrepresented minority retention. 用冠军导向的心态克服研究生院的挑战:研究生院技能研讨会对未被充分代表的少数民族保留的影响。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-08-13 DOI: 10.1093/femspd/ftac024

Andrea G Marshall, Caroline B Palavicino-Maggio, Kit Neikirk, Zer Vue, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton

{"title":"Using champion-oriented mindset to overcome the challenges of graduate school: impact of workshop for graduate school skills on underrepresented minority retention.","authors":"Andrea G Marshall, Caroline B Palavicino-Maggio, Kit Neikirk, Zer Vue, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton","doi":"10.1093/femspd/ftac024","DOIUrl":"https://doi.org/10.1093/femspd/ftac024","url":null,"abstract":"Despite efforts to increase diversity, a glaring underrepresentation of minorities (URM) persists in the fields of science, technology, engineering, and mathematics (STEM). Graduate school can be a stressful step in the STEM pipeline, especially for students previously unaware of the structure and challenges of postgraduate education. To promote successful minority participation in STEM and prepare prospective students for the impending challenges of applying for and attending graduate school, we developed a workshop based on the mentoring and fostering of a champion-oriented mindset entitled, \"The Trials and Tribulations of Graduate School: How Do You Make an Impact?.\" Students from the HBCU Winston-Salem State University attended the workshop, and a pre/post-a 10-point Likert scale-based survey was administered. The questions used in this seminar were newly designed by the authors as program evaluations. The results suggest that the workshop was well-received by the students and provided information that they considered helpful to help navigate the graduate school process.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375733/pdf/ftac024.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10078825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The role of mentoring in promoting diversity equity and inclusion in STEM Education and Research. 指导在促进STEM教育和研究中的多样性公平和包容方面的作用。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-07-21 DOI: 10.1093/femspd/ftac019

Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton

{"title":"The role of mentoring in promoting diversity equity and inclusion in STEM Education and Research.","authors":"Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton","doi":"10.1093/femspd/ftac019","DOIUrl":"https://doi.org/10.1093/femspd/ftac019","url":null,"abstract":"The success of mentoring derives from active and respectful listening and the willingness to learn and accept opportunities for personal growth. This shapes every trainee and their destined path in science, technology, engineering, and mathematics (STEM). The act of cultivating rapport, asking, and pondering meaningful questions, and receiving constructive feedback are critical to support a productive mentoring relationship. Successful mentoring in STEM can be established and allow mentees, especially underrepresented minorities (URMs), to flourish in an environment where they feel welcomed and supported. However, mentees from underrepresented groups often experience inadequate mentoring due to a mentor's lack of awareness, poor trainings themselves, or lack of understanding of the mentee's hardships. It is important for mentors and mentees to work together to promote diversity, equity, and inclusion (DEI) in STEM education through creativity, authenticity, and networking. We analyzed data obtained from students who attended a recent workshop that are interested in going to graduate school. Our results show that despite low initial expectations for the workshop, many students were satisfied in the knowledge they gleaned. The future and role of diversity in STEM within these underrepresented groups lies in community support and an important role that they can play in the lives of others through DEI initiatives and throughout their careers all of which involves positive mentoring.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302695/pdf/ftac019.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9641350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An effective workshop on "How to be an Effective Mentor for Underrepresented STEM Trainees". 一个关于“如何成为STEM受训者的有效导师”的有效研讨会。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-07-06 DOI: 10.1093/femspd/ftac022

Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton

{"title":"An effective workshop on \"How to be an Effective Mentor for Underrepresented STEM Trainees\".","authors":"Andrea G Marshall, Zer Vue, Caroline B Palavicino-Maggio, Kit Neikirk, Heather K Beasley, Edgar Garza-Lopez, Sandra A Murray, Denise Martinez, Amber Crabtree, Zachary C Conley, Larry Vang, Jamaine S Davis, Keesha L Powell-Roach, Susan Campbell, Lillian J Brady, Angyth B Dal, Bryanna Shao, Stefanie Alexander, Nancy Vang, Neng Vue, Mein Vue, Haysetta D Shuler, Elsie C Spencer, Derrick J Morton, Antentor Hinton","doi":"10.1093/femspd/ftac022","DOIUrl":"https://doi.org/10.1093/femspd/ftac022","url":null,"abstract":"Despite an increase in programming to promote persons excluded by their ethnicity or race (PEER) scholars, minorities remain underrepresented in many STEM programs. The academic pipeline is largely leaky for underrepresented minority (URM) scholars due to a lack of effective mentorship. Many URM students experience microaggressions and discrimination from their mentors due to a lack of quality mentorship training. In this workshop, we provide a framework to show trainees what effective mentoring looks like. Mentees, especially URM trainees, can flourish in effective mentoring environments where they feel welcomed and can comfortably develop new ideas without feeling threatened by external factors. Effective mentoring environments provide motivational support, empathy, cultural competency, and training. This workshop explains facets of effective mentoring to students, as well as highlights to URM trainees why mentors can serve as valuable resources.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"80 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258687/pdf/ftac022.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9633385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of concern on: Mitogen-activated protein kinases regulate Mycobacterium avium-induced tumor necrosis factor-α release from macrophages. 对丝裂原活化蛋白激酶调控巨噬细胞释放瘤坏死因子α的关注

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-06-22 DOI: 10.1093/femspd/ftac017

引用次数: 7

Coexistence of blaNDM-1, blaOXA-51, blaOXA-23 and armA in conjunction with novel mutations detected in RND efflux pump regulators in tigecycline resistant clinical isolates of Acinetobacter baumannii. 在对替加环素耐药的鲍曼不动杆菌临床分离株中检测到blaNDM-1、blaOXA-51、blaOXA-23和armA的共存以及RND外排泵调节因子的新突变。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-06-15 DOI: 10.1093/femspd/ftac020

A. Sawant, Sudhakar Pagal, Ashutosh Kumar Amar, L. Panda, S. Devi C., P. Shashikala, R. Kanungo, K. Prashanth

{"title":"Coexistence of blaNDM-1, blaOXA-51, blaOXA-23 and armA in conjunction with novel mutations detected in RND efflux pump regulators in tigecycline resistant clinical isolates of Acinetobacter baumannii.","authors":"A. Sawant, Sudhakar Pagal, Ashutosh Kumar Amar, L. Panda, S. Devi C., P. Shashikala, R. Kanungo, K. Prashanth","doi":"10.1093/femspd/ftac020","DOIUrl":"https://doi.org/10.1093/femspd/ftac020","url":null,"abstract":"This study has investigated a total of 51 A. baumannii isolates for the prevalence of resistant determinants in tigecycline susceptible and non-susceptible clinical isolates of A. baumannii. Antimicrobial susceptibility testing revealed 74% of isolates were tigecycline resistant. Mutations in RND-efflux pump regulatory genes and the expression of efflux pump genes were measured in tigecycline resistant isolates. There was a strong co-relation between the blaNDM-1 and armA wherein majority of the isolates that are positive for blaNDM-1 have also harbored armA. Compared with TSAB (tigecycline susceptible A. baumannii), TNAB (tigecycline non-susceptible A. baumannii) isolates show increased distribution of blaNDM-1 (p = 0.048), blaIMP-1 (p<0.0001) and blaOXA-51 (p = 0.0029) carbapenemase genes. The variants of RND-efflux pump regulatory genes due to amino-acid mutations in adeS (F12S, K84E, W61R, N268H and Q299R) and adeL (G21R and Q262R) were identified in tigecycline resistant isolates as well as ISAba1 mediated disruption of adeN were observed causing overexpression of adeIJK efflux pump. Additionally, mutations in adeRS were also associated with increased expression of adeABC efflux pump. Besides, TNAB isolates showed significantly (p<0.0001) higher ability of biofilm formation as compared to TSAB isolates. The tigecycline resistance due to mutations in contemporary A. baumannii isolates having a higher ability to form biofilm may pose therapeutic difficulties.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48021488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunopotentiation by linking Hsp70 T-cell epitopes to Gag-Pol-Env-Nef-Rev multiepitope construct and increased IFN-gamma secretion in infected lymphocytes. 通过将Hsp70 T细胞表位连接到Gag-Pol-Ev-Nef-Rev多表位构建体来增强免疫，并增加感染淋巴细胞中的IFN-γ分泌。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-06-15 DOI: 10.1093/femspd/ftac021

E. Akbari, S. Ajdary, E. M. Ardakani, Elnaz Agi, A. Milani, Masoud Seyedinkhorasani, V. Khalaj, A. Bolhassani

{"title":"Immunopotentiation by linking Hsp70 T-cell epitopes to Gag-Pol-Env-Nef-Rev multiepitope construct and increased IFN-gamma secretion in infected lymphocytes.","authors":"E. Akbari, S. Ajdary, E. M. Ardakani, Elnaz Agi, A. Milani, Masoud Seyedinkhorasani, V. Khalaj, A. Bolhassani","doi":"10.1093/femspd/ftac021","DOIUrl":"https://doi.org/10.1093/femspd/ftac021","url":null,"abstract":"Therapeutic human immunodeficiency virus (HIV) vaccines can boost the anti-HIV host immunity to control viral replication and eliminate viral reservoirs in the absence of anti-retroviral therapy. In this study, two computationally designed multiepitope Gag-Pol-Env-Nef-Rev and Hsp70-Gag-Pol-Env-Nef-Rev constructs harboring immunogenic and highly conserved HIV T cell epitopes were generated in E. coli as polypeptide vaccine candidates. Furthermore, the multiepitope gag-pol-env-nef-rev and hsp70-gag-pol-env-nef-rev DNA vaccine constructs were prepared and complexed with MPG cell-penetrating peptide. The immunogenicity of the multiepitope constructs were evaluated using the homologous and heterologous prime/boost strategies in mice. Moreover, the secretion of IFN-γ was assessed in infected lymphocytes in vitro. Our data showed that the homologous polypeptide regimens could significantly induce a mixture of IgG1 and IgG2a antibody responses, activate T cells to secret IFN-γ, IL-5, IL-10, and generate Granzyme B. Moreover, IFN-γ secretion was significantly enhanced in single-cycle replicable (SCR) HIV-1 virions-infected splenocytes in these groups compared to uninfected splenocytes. The linkage of heat shock protein 70 (Hsp70) epitopes to Gag-Pol-Env-Nef-Rev polypeptide in the homologous regimen increased significantly cytokines and Granzyme B levels, and IFN-γ secretion in virions-infected splenocytes. Briefly, both designed constructs in the homologous regimens can be used as a promising vaccine candidate against HIV infection.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47088597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1