Pathogens and disease最新文献_第7页

Assessment of long-term adverse events regarding different COVID-19 vaccine regimens within an 18-month follow-up study. 在18个月的随访研究中评估不同COVID-19疫苗方案的长期不良事件

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad010

Mona Sadat Larijani, Rahim Sorouri, Sana Eybpoosh, Delaram Doroud, Ladan Moradi, Mozhgan Ahmadinezhad, Anahita Bavand, Fatemeh Ashrafian, Parinaz Tajmehrabi Namini, Mahsan Zali, Amitis Ramezani

{"title":"Assessment of long-term adverse events regarding different COVID-19 vaccine regimens within an 18-month follow-up study.","authors":"Mona Sadat Larijani, Rahim Sorouri, Sana Eybpoosh, Delaram Doroud, Ladan Moradi, Mozhgan Ahmadinezhad, Anahita Bavand, Fatemeh Ashrafian, Parinaz Tajmehrabi Namini, Mahsan Zali, Amitis Ramezani","doi":"10.1093/femspd/ftad010","DOIUrl":"https://doi.org/10.1093/femspd/ftad010","url":null,"abstract":"Early reports on coronavirus disease 2019 (COVID-19) vaccines presented the short-term adverse events (AEs). This follow-up study investigated a standard regimen based on protein subunit vaccines, PastoCovac and PastoCovac Plus, and the combinational vaccine regimens including AstraZeneca/PastoCovac Plus and Sinopharm/PastoCovac Plus. The participants were followed up to 6 months post the booster shot. All the AEs were collected through in-depth interviews using a valid researcher-made questionnaire and were evaluated regarding the association with the vaccines. Of the 509 individuals, 6.2% of the combinational vaccine participants had late AEs, of whom 3.3% suffered from cutaneous manifestations, followed by 1.1% arthralgia complaints, 1.1% with neurologic disorders, 0.3% ocular problems and 0.3% metabolic complications, with no significant difference between the vaccine regimens. For the standard regimen, 2% of the individuals experienced late AEs as (1%), neurological disorders (0.3%), metabolic problems (0.3%) and involvement of joints (0.3%). Notably, 75% of the AEs were persistent up to the end of the study. A low number of late AEs were captured in 18 months as 12 improbable, 5 unclassifiable, 4 possible and 3 probable associated AEs with the vaccine regimens. The benefits of COVID-19 vaccination far exceed the potential risks and late AEs seem to be uncommon.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Immunogenicity evaluation of a novel virus-like particle vaccine candidate against SARS-CoV-2 in BALB/c. 针对严重急性呼吸系统综合征冠状病毒2型的新型病毒样颗粒候选疫苗在BALB/c中的免疫原性评估。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad021

Golnaz Bahramali, Maryam Mashhadi Abolghasem Shirazi, Mina Hannan, Mohammad Reza Aghasadeghi, Mohammad Sadeq Khosravy, Sina Arjmand, Seyed Mehdi Sadat

{"title":"Immunogenicity evaluation of a novel virus-like particle vaccine candidate against SARS-CoV-2 in BALB/c.","authors":"Golnaz Bahramali, Maryam Mashhadi Abolghasem Shirazi, Mina Hannan, Mohammad Reza Aghasadeghi, Mohammad Sadeq Khosravy, Sina Arjmand, Seyed Mehdi Sadat","doi":"10.1093/femspd/ftad021","DOIUrl":"10.1093/femspd/ftad021","url":null,"abstract":"The coronavirus disease (COVID-19) pandemic has imposed deployment of an effective vaccine as a worldwide health priority. The new variants of SARS-CoV-2 have also brought serious concerns due to virus eradiation hesitancy. In this study, we evaluated the protective immune system activity of a recombinant viral vector-based vaccine candidate encoding a fusion spike, membrane and nucleocapsid proteins, Spike (528-1273aa)-M-N, in BALB/c via two different routes of delivery, intranasal and subcutaneous. The immune responses were then assessed through specific SARS-CoV-2 antibodies, interleukin and granzyme B secretion. The outcomes showed that the IgG titer and IgA secretion was higher in intranasal route in comparison with the subcutaneous, and what is more, a higher titer of IL-4 was detected through the intranasal route, whereas IFN-γ was highly induced via the subcutaneous route. The cytotoxic cell activities were mostly achieved via subcutaneous route immunization. Vaccination with the target antigen is immunogenic and led to induction of specific antibodies. Both humoral and cellular immunity arms were well activated in immunized mice, especially through intranasal route with detectable IgA and IgG. Therefore, implication of the platform as a potential vaccine candidate has potential as a future prophylactic vaccine that guarantees further investigations for the assessment of its immunogenicity in humans.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10155293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Burkholderia contaminans prevalent phenotypes as possible markers of poor clinical outcomes in chronic lung infection of children with cystic fibrosis. 在囊性纤维化儿童慢性肺部感染中，伯克霍尔德菌污染的流行表型可能是不良临床结果的标志。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad003

Beltina León, Claudia Prieto, Marisa Bettiol, Mariana Leguizamón, Virginia D Alessandro, Daniela Casco, Carolina Vita, Cecilia Beatriz Figoli, Cecilia Vescina, Fernando Rentería, Silvia T Cardona, Alejandra Bosch

{"title":"The Burkholderia contaminans prevalent phenotypes as possible markers of poor clinical outcomes in chronic lung infection of children with cystic fibrosis.","authors":"Beltina León, Claudia Prieto, Marisa Bettiol, Mariana Leguizamón, Virginia D Alessandro, Daniela Casco, Carolina Vita, Cecilia Beatriz Figoli, Cecilia Vescina, Fernando Rentería, Silvia T Cardona, Alejandra Bosch","doi":"10.1093/femspd/ftad003","DOIUrl":"https://doi.org/10.1093/femspd/ftad003","url":null,"abstract":"Burkholderia contaminans, a species of the Burkholderia cepacia complex-prevalent in certain Latin-American and European countries-can cause chronic pulmonary infection in persons with cystic fibrosis. Our aim was to gain insights into long-term lung infections with a focus on correlating how bacterial phenotypic traits in the chronic infection impact on patients' clinical outcome. Genotypic characteristics of 85 B. contaminans isolates recovered from 70 patients were investigated. For 16 of those patients, the clinical status and bacterial phenotypic characteristics, e.g. several virulence factors, phenotypic variants, and the antimicrobial susceptibility pattern, were evaluated. Two clones were found in the whole bacterial population: (i) the multiresistant ST 872 PCR-recA-RFLP-HaeIII-K-pattern clone, which carries a pathogenic island homologous to BcenGI11 of B. cenocepacia J2315, and (ii) the ST 102 PCR-recA-RFLP-HaeIII-AT-pattern clone. The emergence of certain bacterial phenotypes in the chronic infection such as the nonmucoid phenotype, small colony variants, brownish pigmented colonies, and hypermutators, proved to be, together with coinfection with Pseudomonas aeruginosa, the possible markers of more challenging infections and poor prognosis. The presence of cocolonizers and the bacterial phenotypes that are especially adapted to persist in long-term respiratory tract infections have a crucial role in patients' clinical outcomes.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9819201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Hypoxia-inducible factor 1α in host defense during pneumococcal pneumonia. 缺氧诱导因子1α在肺炎球菌肺炎中宿主防御中的作用。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftac047

Liza Pereverzeva, Natasja A Otto, Hessel Peters-Sengers, Joris J T H Roelofs, Alex F de Vos, Tom van der Poll

{"title":"Role of Hypoxia-inducible factor 1α in host defense during pneumococcal pneumonia.","authors":"Liza Pereverzeva, Natasja A Otto, Hessel Peters-Sengers, Joris J T H Roelofs, Alex F de Vos, Tom van der Poll","doi":"10.1093/femspd/ftac047","DOIUrl":"https://doi.org/10.1093/femspd/ftac047","url":null,"abstract":"Hypoxia-inducible factor (HIF)1α is a transcription factor involved in cellular metabolism and regulation of immune cell effector functions. Here, we studied the role of HIF1α in myeloid cells during pneumonia caused by the major causative pathogen, Streptococcus pneumoniae (Spneu). Mice deficient for HIF1α in myeloid cells (LysMcreHif1αfl/fl) were generated to study the in vitro responsiveness of bone marrow-derived macrophages (BMDMs) and alveolar macrophages (AMs) to the Gram-positive bacterial wall component lipoteichoic acid (LTA) and heat-killed Spneu, and the in vivo host response after infection with Spneu via the airways. Both BMDMs and AMs released more lactate upon stimulation with LTA or Spneu, indicative of enhanced glycolysis; HIF1α-deficiency in these cells was associated with diminished lactate release. In BMDMs, HIF1α-deficiency resulted in reduced secretion of tumor necrosis factor (TNF)α and interleukin (IL)-6 upon activation with Spneu but not LTA, while HIF1α-deficient AMs secreted less TNFα and IL-6 in response to LTA, and TNFα after Spneu stimulation. However, no difference was found in the host response of LysMcreHif1αfl/fl mice after Spneu infection as compared to controls. Similar in vivo findings were obtained in neutrophil (Mrp8creHif1αfl/fl) HIF1α-deficient mice. These data suggest that myeloid HIF1α is dispensable for the host defense during pneumococcal pneumonia.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10817697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First molecular detection of Entamoeba gingivalis subtypes in individuals from Turkey. 首次在土耳其个体中进行齿龈内阿米巴亚型的分子检测。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad017

Serra Örsten, Cem Şahin, Engin Yılmaz, Yakut Akyön

{"title":"First molecular detection of Entamoeba gingivalis subtypes in individuals from Turkey.","authors":"Serra Örsten, Cem Şahin, Engin Yılmaz, Yakut Akyön","doi":"10.1093/femspd/ftad017","DOIUrl":"10.1093/femspd/ftad017","url":null,"abstract":"Entamoeba gingivalis is a parasitic protozoan that colonizes the human oral cavity and there are two subtypes (ST1 and ST2) that have been identified to date. However, there are no reports on the molecular detection or characterization of E. gingivalis in Turkey. The objective of this study was to detect the presence of E. gingivalis in Turkish healthy individuals and those with periodontal disease and to subtype the isolates using molecular techniques. Samples from the oral cavity of 94 individuals were taken and the presence of E. gingivalis was determined by PCR using primers for SsrRNA and the amplicons were then confirmed by DNA sequencing. Each participant completed a questionnaire that included demographic data, habits and lifestyle, as well as health status. The presence of E. gingivalis was detected in a total of 19 samples (11 patients and eight healthy individuals). Molecular characterization determined that 12 samples belonged to ST1 and seven samples belonged to ST2. The presence of E. gingivalis was higher in patients with periodontal disease than in healthy individuals, and this association was statistically significant (P < .05). This study constitutes the first report of molecular detection and subtyping of E. gingivalis in Turkey.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10553396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10275595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nur77 influences immunometabolism to regulate the release of proinflammatory cytokines and the formation of lipid bodies during Mycobacterium tuberculosis infection of macrophages. Nur77在结核分枝杆菌感染巨噬细胞过程中影响免疫代谢，调节促炎细胞因子的释放和脂质体的形成。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad033

Pankaj Birari, Soumya Mal, Debayan Majumder, Arun K Sharma, Manish Kumar, Troyee Das, Zhumur Ghosh, Kuladip Jana, Umesh D Gupta, Manikuntala Kundu, Joyoti Basu

{"title":"Nur77 influences immunometabolism to regulate the release of proinflammatory cytokines and the formation of lipid bodies during Mycobacterium tuberculosis infection of macrophages.","authors":"Pankaj Birari, Soumya Mal, Debayan Majumder, Arun K Sharma, Manish Kumar, Troyee Das, Zhumur Ghosh, Kuladip Jana, Umesh D Gupta, Manikuntala Kundu, Joyoti Basu","doi":"10.1093/femspd/ftad033","DOIUrl":"10.1093/femspd/ftad033","url":null,"abstract":"Infection of macrophages with Mycobacterium tuberculosis induces innate immune responses designed to clear the invading bacterium. However, bacteria often survive within the intracellular environment by exploiting these responses triggered by macrophages. Here, the role of the orphan nuclear receptor Nur77 (Nr4a1) in regulating the response of macrophages infected with M. tuberculosis (Mtb) has been delineated. Nur77 is induced early during infection, regulates metabolism by binding directly at the promoter of the TCA cycle enzyme, isocitrate dehydrogenase 2 (IDH2), to act as its repressor, and shifts the balance from a proinflammatory to an anti-inflammatory phenotype. Depletion of Nur77 increased transcription of IDH2 and, consequently, the levels of intracellular succinate, leading to enhanced levels of the proinflammatory cytokine IL-1β. Further, Nur77 inhibited the production of antibacterial nitric oxide and IL-1β in a succinate dehydrogenase (SDH)-dependent manner, suggesting that its induction favors bacterial survival by suppressing bactericidal responses. Indeed, depletion of Nur77 inhibited the intracellular survival of Mtb. On the other hand, depletion of Nur77 enhanced lipid body formation, suggesting that the fall in Nur77 levels as infection progresses likely favors foamy macrophage formation and long-term survival of Mtb in the host milieu.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138452018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of nutrients on the function of the chlamydial Rsb partner switching mechanism. 营养物质对衣原体Rsb伴侣转换机制功能的影响。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-11-29 DOI: 10.1093/femspd/ftac044

Shiomi Kuwabara, Evan R Landers, Derek J Fisher

{"title":"Impact of nutrients on the function of the chlamydial Rsb partner switching mechanism.","authors":"Shiomi Kuwabara, Evan R Landers, Derek J Fisher","doi":"10.1093/femspd/ftac044","DOIUrl":"https://doi.org/10.1093/femspd/ftac044","url":null,"abstract":"The obligate intracellular bacterial pathogen Chlamydia trachomatis is a leading cause of sexually transmitted infections and infectious blindness. Chlamydia undergo a biphasic developmental cycle alternating between the infectious elementary body (EB) and the replicative reticulate body (RB). The molecular mechanisms governing RB growth and RB-EB differentiation are unclear. We hypothesize that the bacterium senses host cell and bacterial energy levels and metabolites to ensure that development and growth coincide with nutrient availability. We predict that a partner switching mechanism (PSM) plays a key role in the sensing and response process acting as a molecular throttle sensitive to metabolite levels. Using purified wild type and mutant PSM proteins, we discovered that metal type impacts enzyme activity and the substrate specificity of RsbU and that RsbW prefers ATP over GTP as a phosphate donor. Immunoblotting analysis of RsbV1/V2 demonstrated the presence of both proteins beyond 20 hours post infection and we observed that an RsbV1-null strain has a developmental delay and exhibits differential growth attenuation in response to glucose levels. Collectively, our data support that the PSM regulates growth in response to metabolites and further defines biochemical features governing PSM-component interactions which could help in the development of novel PSM-targeted therapeutics.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10343554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyp25α is required for male gametocyte exflagellation. Pyp25α是雄性配子体脱落所必需的。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-11-12 DOI: 10.1093/femspd/ftac043

Chao Zhang, Deyi Li, Zhirong Meng, Jianwei Zhou, Zhenxiao Min, Shengqun Deng, Jijia Shen, Miao Liu

{"title":"Pyp25α is required for male gametocyte exflagellation.","authors":"Chao Zhang, Deyi Li, Zhirong Meng, Jianwei Zhou, Zhenxiao Min, Shengqun Deng, Jijia Shen, Miao Liu","doi":"10.1093/femspd/ftac043","DOIUrl":"https://doi.org/10.1093/femspd/ftac043","url":null,"abstract":"Malaria, a mosquito-borne infectious disease, is caused by the unicellular apicomplexan protozoa of the genus Plasmodium. For malaria parasite transmission, the essential sexual stage includes production of gametocytes through gametocytogenesis in vertebrate hosts and formation of gametes from gametocytes through gametogenesis in mosquito vectors. Whereas each female gametocyte forms a single immotile macrogamete, a male gametocyte produces eight flagella-like microgametes in a process called exflagellation. We identified a conserved protein named as Py05543 (Pyp25α), required for male gametocyte exflagellation in Plasmodium yoelii, which is the ortholog of PFL1770c (PF3D7_1236600). Interestingly, PF3D7_1236600 was previously phenotypically screened to be gametocyte-essential genes during gametocytogenesis of Plasmodium falciparum, using piggyBac transposon-mediated insertional mutagenesis. In this study, using CRISPR/Cas9-mediated genome editing, the Pyp25α¯ (KO) parasite line was successfully established. We found that the KO parasites proliferated asexually in mouse blood normally. In addition, compared with that of the parental parasites, the KO parasites displayed similar levels of gametocytes formation. Unexpectedly, the KO parasites showed considerable deficiency in exflagellation of male gametes, by observing exflagellation centre formation. Taken together, our data suggested that Pyp25α gene, the ortholog of PF3D7_1236600, was nonessential for the growth of asexual parasites, required for male gametocyte exflagellation in P. yoelii.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40446813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Trichinella spiralis-derived extracellular vesicles induce a protective immunity against larval challenge in mice. 旋毛虫衍生的细胞外囊泡诱导小鼠对幼虫攻击的保护性免疫。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-11-12 DOI: 10.1093/femspd/ftac040

Dalia S Ashour, Fatma M Kh Ibrahim, Amira M Elshamy, Hager S Zoghroban

{"title":"Trichinella spiralis-derived extracellular vesicles induce a protective immunity against larval challenge in mice.","authors":"Dalia S Ashour, Fatma M Kh Ibrahim, Amira M Elshamy, Hager S Zoghroban","doi":"10.1093/femspd/ftac040","DOIUrl":"https://doi.org/10.1093/femspd/ftac040","url":null,"abstract":"Human trichinellosis is a serious disease with no effective treatment till now. Recently, the protective immunity induced by parasite-derived extracellular vesicles (EVs) are studied for some parasites such as Echinostoma caproni. The current study aimed to investigate the novel Trichinella spiralis-derived EVs as a potential vaccine candidate for the first time in a mouse model. Trichinella spiralis EVs were isolated and identified using transmission electron microscopy, gel electrophoresis, protein content measurements, and beads-based flow cytometry. Vaccination was done by subcutaneous injection of two doses of 3.5 μg T. spiralis-derived EVs. We observed a significant reduction in T. spiralis adult worm and muscle larval counts in mice immunized with T. spiralis-derived EVs (EVs-Ts group) and controlled inflammatory changes in the intestine and muscles. The EVs-Ts group showed a higher level of IFN- γ, whereas the IL-4 secretion was elevated more in the EVs group (EVs group) and showed a lower level after challenge with T. spiralis infection (EVs-Ts group). This implies a mixed Th1/Th2 immune response with obvious Th1 polarization. Moreover, elevation of serum T. spiralis-specific IgG was reported. In conclusion, this preliminary study provides T. spiralis EVs as a promising candidate for future development of anti-Trichinella vaccine.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40557786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Proteomic profiling of extracellular vesicles derived from ARPE-19 cells challenged with Aspergillus flavus and Candida albicans: application in fungal endophthalmitis. 黄曲霉和白色念珠菌攻毒的ARPE-19细胞外囊泡的蛋白质组学分析:在真菌性眼内炎中的应用

IF 3.3 4区医学

Pathogens and disease Pub Date : 2022-11-02 DOI: 10.1093/femspd/ftac042

Jaishree Gandhi, Joveeta Joseph

{"title":"Proteomic profiling of extracellular vesicles derived from ARPE-19 cells challenged with Aspergillus flavus and Candida albicans: application in fungal endophthalmitis.","authors":"Jaishree Gandhi, Joveeta Joseph","doi":"10.1093/femspd/ftac042","DOIUrl":"https://doi.org/10.1093/femspd/ftac042","url":null,"abstract":"Extracellular vesicles (EVs) are nano-sized-particles that play an important role in cellular cross-talk. The aim of this study was to understand the proteomic cargo of EVs, released by Retinal Pigment Epithelial (RPE) cells challenged with Candida albicans (C-CA) and Aspergillus flavus (C-AF). EVs were isolated from culture supernatant of retinal cells infected with fungal pathogens and characterized by dynamic light scattering, SEM, and western blot. EV proteome was then evaluated by mass spectrometry (LC-MS/MS). Isolated EVs were approximately 120-150 nm and higher in number in infected group compared to control. Proteomic profiling of EVs from infected cells, showed a total of 419 and 254 differentially expressed proteins, of which 218 were upregulated in C-CA group and 81 proteins were upregulated in C-AF group. Gene ontology revealed majority of proteins associated with transport, cell migration, and in activation of innate immune response. Proteins identified were annexins, calpain, and Sorcin proteins. Additionally, KEGG analysis unveiled involvement of MAPK, HIF-1, and PI3K-AKT signalling pathways. Proteomic results indicate that EVs cargo derived from fungal-infected retinal cells can activate immune signalling pathways and might contribute to the pathogenesis of endophthalmitis, indicating the potential use of EVs as theranostic marker for management of fungal infections.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40428584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2