Pathogens and disease最新文献_第7页

Chlamydia pneumoniae-immunoglobulin E antibody responses in serum from children with asthma. 哮喘患儿血清肺炎衣原体免疫球蛋白E抗体反应。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad015

Tamar A Smith-Norowitz, Anastasiya Shulman, Haram Abdelmajid, Margaret R Hammerschlag, Rauno Joks, Diana Weaver, Stephan Kohlhoff

{"title":"Chlamydia pneumoniae-immunoglobulin E antibody responses in serum from children with asthma.","authors":"Tamar A Smith-Norowitz, Anastasiya Shulman, Haram Abdelmajid, Margaret R Hammerschlag, Rauno Joks, Diana Weaver, Stephan Kohlhoff","doi":"10.1093/femspd/ftad015","DOIUrl":"https://doi.org/10.1093/femspd/ftad015","url":null,"abstract":"Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infections in humans. An association between persistent C. pneumoniae infection and asthma pathogenesis has been described. It is unknown whether specific immunoglobulin E (IgE) is a marker of persistent immune activation responses. Therefore, the association between C. pneumoniae-specific-IgE antibodies (Abs) and interferon (IFN)-gamma produced by C. pneumoniae-stimulated peripheral blood mononuclear cells (PBMC) was examined. Blood was collected and serum separated. PBMC from 63 children with or without stable asthma (N = 45 and 18, respectively) were infected or not infected with C. pneumoniae AR-39 and cultured for up to 7 days. Supernatants were collected, and IFN-gamma levels measured (ELISA). Serum C. pneumoniae-IgE Abs were detected by immunoblotting. C. pneumoniae-IgE Abs were detected in asthmatics (27%), compared with non-asthmatics (11%) (P = NS). IFN-gamma responses were more prevalent among asthmatics who had positive C. pneumoniae-IgE Abs (60%) compared with asthmatics without C. pneumoniae-IgE Abs (20%) (P = 0.1432). IFN-gamma responses in C. pneumoniae-stimulated PBMC from children with asthma were more frequent in children who had specific anti-C. pneumoniae-IgE Abs compared to those who did not. This immune response may reflect persistent infection, which may contribute to ongoing asthma symptoms.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9897881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infectious and environmental placental insults: from underlying biological pathways to diagnostics and treatments. 感染性和环境性胎盘损伤：从潜在的生物学途径到诊断和治疗。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad024

Samuel Chenge, Harrison Ngure, Bernard N Kanoi, Amanda N Sferruzzi-Perri, Francis M Kobia

{"title":"Infectious and environmental placental insults: from underlying biological pathways to diagnostics and treatments.","authors":"Samuel Chenge, Harrison Ngure, Bernard N Kanoi, Amanda N Sferruzzi-Perri, Francis M Kobia","doi":"10.1093/femspd/ftad024","DOIUrl":"10.1093/femspd/ftad024","url":null,"abstract":"Because the placenta is bathed in maternal blood, it is exposed to infectious agents and chemicals that may be present in the mother's circulation. Such exposures, which do not necessarily equate with transmission to the fetus, may primarily cause placental injury, thereby impairing placental function. Recent research has improved our understanding of the mechanisms by which some infectious agents are transmitted to the fetus, as well as the mechanisms underlying their impact on fetal outcomes. However, less is known about the impact of placental infection on placental structure and function, or the mechanisms underlying infection-driven placental pathogenesis. Moreover, recent studies indicate that noninfectious environmental agents accumulate in the placenta, but their impacts on placental function and fetal outcomes are unknown. Critically, diagnosing placental insults during pregnancy is very difficult and currently, this is possible only through postpartum placental examination. Here, with emphasis on humans, we discuss what is known about the impact of infectious and chemical agents on placental physiology and function, particularly in the absence of maternal-fetal transmission, and highlight knowledge gaps with potential implications for diagnosis and intervention against placental pathologies.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Murine herpesvirus-68-related growth factors treatment correlates with decrease of p53 and HIF-1α protein levels. 小鼠疱疹病毒-68相关生长因子治疗与p53和HIF-1α蛋白水平降低相关。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad004

Miroslava Supolikova, Eva Novakova, Karin Donatova, Petra Olejnikova, Martina Labudova

{"title":"Murine herpesvirus-68-related growth factors treatment correlates with decrease of p53 and HIF-1α protein levels.","authors":"Miroslava Supolikova, Eva Novakova, Karin Donatova, Petra Olejnikova, Martina Labudova","doi":"10.1093/femspd/ftad004","DOIUrl":"https://doi.org/10.1093/femspd/ftad004","url":null,"abstract":"Murine herpesvirus 68 (MHV-68) belongs to the subfamily Gammaherpesvirinae of the family Herpesviridae. This exceptional murine herpesvirus is an excellent model for the study of human gammaherpesvirus infections. Cells infected with MHV-68 under nonpermissive conditions for viral replication produce substances designated as MHV-68 growth factors (MHGF-68), that can cause transformation of the cells, or on the other side, turn transformed cells into normal. It was already proposed, that the MHGF-68 fractions cause transformation, disruption of the cytoskeleton and slower growth of the tumors in nude mice. Here, we examined newly extracted fractions of MHGF-68 designated as F5 and F8. Both fractions proved to inhibit the growth of the spheroids and also tumours induced in nude mice. What more, the fractions caused the decrease of the protein levels of wt p53 and HIF-1α. Decreased levels of p53 and HIF-1α activity leads to decreased vascularization, slower tumour growth, and lower adaptation to hypoxic conditions. This would propose MHGF-68 fractions, or their human herpesvirus equivalents, as a potential anticancer drugs in combined chemotherapy.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f6/63/ftad004.PMC10093997.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9465929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative profile, inflammatory responses and δ-aminolevulinate dehydratase enzyme activity in influenza B virus infection. 乙型流感病毒感染的氧化特性、炎症反应和δ-氨基乙酰丙酸脱水酶活性。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad028

Jovana Simonetti Bulegon, Andressa de Azambuja Pias Weber, Manoela Dias de Souza, Fernanda Tibolla Viero, Micheli Mainardi Pillat, Thissiane de Lima Gonçalves

{"title":"Oxidative profile, inflammatory responses and δ-aminolevulinate dehydratase enzyme activity in influenza B virus infection.","authors":"Jovana Simonetti Bulegon, Andressa de Azambuja Pias Weber, Manoela Dias de Souza, Fernanda Tibolla Viero, Micheli Mainardi Pillat, Thissiane de Lima Gonçalves","doi":"10.1093/femspd/ftad028","DOIUrl":"10.1093/femspd/ftad028","url":null,"abstract":"The aim of the current study was to determine the activity of the delta-aminolevulinate dehydratase (δ-ALA-D) enzyme, oxidative stress biomarkers and the expression of cytokines in those infected with influenza B virus (IBV). To evaluate the activity of the δ-ALA-D enzyme, lipid peroxidation was estimated as levels of thiobarbituric acid reactive substances, protein and non-protein thiol groups, ferric-reducing antioxidant power (FRAP), vitamin C concentration and cytokine levels in IBV-infected individuals (n = 50) and a control group (n = 30). δ-ALA-D activity was significantly lower in IBV-infected individuals compared with controls, as well as levels of thiols, vitamin C and FRAP. Lipid peroxidation and cytokine levels of IL-6, IL-10, IL-17A and IFN-y were statistically higher in the IBV group. In conclusion, we found evidence of the generation of oxidants, the depletion of the antioxidant system, decrease in the activity of the δ-ALA-D enzyme and an increase in the synthesis of cytokines, thus contributing to a better understanding of oxidative and inflammatory pathways during IBV infection.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41208257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics in vaginal health. 阴道健康中的益生菌。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad012

Noushin Mashatan, Reza Heidari, Mana Altafi, Amir Amini, Mohammad Mehdi Ommati, Masoud Hashemzaei

{"title":"Probiotics in vaginal health.","authors":"Noushin Mashatan, Reza Heidari, Mana Altafi, Amir Amini, Mohammad Mehdi Ommati, Masoud Hashemzaei","doi":"10.1093/femspd/ftad012","DOIUrl":"10.1093/femspd/ftad012","url":null,"abstract":"Bacterial vaginosis, a type of vaginal inflammation, can be considered the main reason for abnormal discharges of the vagina and vaginal dysbiosis during reproductive years. Epidemiological investigations of females suffering from vaginitis demonstrated that at least 30% to 50% of all women had Bacterial vaginosis (BV). One of the fields of treatment is the use of probiotics, probiotics are commonly defined as viable microorganisms (yeasts or bacteria) that can positively affect the health of their hosts. They are used in foods, notably fermented milk products, and medicine-related products. The development of new probiotic strains is aimed at more active advantageous organisms. Lactobacillus species are the dominant bacteria in a normal vagina that can decrease the pH of the vagina by the production of lactic acid. A number of lactobacilli types can produce hydrogen peroxide as well. The presence of hydrogen peroxide-induced low pH can prevent the growth of several other microorganisms. The vaginal flora of BV cases can modify by replacing the Lactobacillus species with a high density of anaerobic bacteria (i.e. Mobiluncus sp. Bacteroides sp.), Mycoplasma hominis, and Gardnerella vaginalis. More vaginal infections are treated with medications, while there is a possibility of recurrence and chronic infection because of the adverse effects on the indigenous lactobacilli. Probiotics and prebiotics have shown capacities for optimizing, maintaining, and restoring the vaginal microflora. Therefore, biotherapeutics can offer alternative approaches to reduce infections of the vagina and thus promote consumers' health.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9796881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Burkholderia contaminans prevalent phenotypes as possible markers of poor clinical outcomes in chronic lung infection of children with cystic fibrosis. 在囊性纤维化儿童慢性肺部感染中，伯克霍尔德菌污染的流行表型可能是不良临床结果的标志。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad003

Beltina León, Claudia Prieto, Marisa Bettiol, Mariana Leguizamón, Virginia D Alessandro, Daniela Casco, Carolina Vita, Cecilia Beatriz Figoli, Cecilia Vescina, Fernando Rentería, Silvia T Cardona, Alejandra Bosch

{"title":"The Burkholderia contaminans prevalent phenotypes as possible markers of poor clinical outcomes in chronic lung infection of children with cystic fibrosis.","authors":"Beltina León, Claudia Prieto, Marisa Bettiol, Mariana Leguizamón, Virginia D Alessandro, Daniela Casco, Carolina Vita, Cecilia Beatriz Figoli, Cecilia Vescina, Fernando Rentería, Silvia T Cardona, Alejandra Bosch","doi":"10.1093/femspd/ftad003","DOIUrl":"https://doi.org/10.1093/femspd/ftad003","url":null,"abstract":"Burkholderia contaminans, a species of the Burkholderia cepacia complex-prevalent in certain Latin-American and European countries-can cause chronic pulmonary infection in persons with cystic fibrosis. Our aim was to gain insights into long-term lung infections with a focus on correlating how bacterial phenotypic traits in the chronic infection impact on patients' clinical outcome. Genotypic characteristics of 85 B. contaminans isolates recovered from 70 patients were investigated. For 16 of those patients, the clinical status and bacterial phenotypic characteristics, e.g. several virulence factors, phenotypic variants, and the antimicrobial susceptibility pattern, were evaluated. Two clones were found in the whole bacterial population: (i) the multiresistant ST 872 PCR-recA-RFLP-HaeIII-K-pattern clone, which carries a pathogenic island homologous to BcenGI11 of B. cenocepacia J2315, and (ii) the ST 102 PCR-recA-RFLP-HaeIII-AT-pattern clone. The emergence of certain bacterial phenotypes in the chronic infection such as the nonmucoid phenotype, small colony variants, brownish pigmented colonies, and hypermutators, proved to be, together with coinfection with Pseudomonas aeruginosa, the possible markers of more challenging infections and poor prognosis. The presence of cocolonizers and the bacterial phenotypes that are especially adapted to persist in long-term respiratory tract infections have a crucial role in patients' clinical outcomes.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9819201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity evaluation of a novel virus-like particle vaccine candidate against SARS-CoV-2 in BALB/c. 针对严重急性呼吸系统综合征冠状病毒2型的新型病毒样颗粒候选疫苗在BALB/c中的免疫原性评估。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad021

Golnaz Bahramali, Maryam Mashhadi Abolghasem Shirazi, Mina Hannan, Mohammad Reza Aghasadeghi, Mohammad Sadeq Khosravy, Sina Arjmand, Seyed Mehdi Sadat

{"title":"Immunogenicity evaluation of a novel virus-like particle vaccine candidate against SARS-CoV-2 in BALB/c.","authors":"Golnaz Bahramali, Maryam Mashhadi Abolghasem Shirazi, Mina Hannan, Mohammad Reza Aghasadeghi, Mohammad Sadeq Khosravy, Sina Arjmand, Seyed Mehdi Sadat","doi":"10.1093/femspd/ftad021","DOIUrl":"10.1093/femspd/ftad021","url":null,"abstract":"The coronavirus disease (COVID-19) pandemic has imposed deployment of an effective vaccine as a worldwide health priority. The new variants of SARS-CoV-2 have also brought serious concerns due to virus eradiation hesitancy. In this study, we evaluated the protective immune system activity of a recombinant viral vector-based vaccine candidate encoding a fusion spike, membrane and nucleocapsid proteins, Spike (528-1273aa)-M-N, in BALB/c via two different routes of delivery, intranasal and subcutaneous. The immune responses were then assessed through specific SARS-CoV-2 antibodies, interleukin and granzyme B secretion. The outcomes showed that the IgG titer and IgA secretion was higher in intranasal route in comparison with the subcutaneous, and what is more, a higher titer of IL-4 was detected through the intranasal route, whereas IFN-γ was highly induced via the subcutaneous route. The cytotoxic cell activities were mostly achieved via subcutaneous route immunization. Vaccination with the target antigen is immunogenic and led to induction of specific antibodies. Both humoral and cellular immunity arms were well activated in immunized mice, especially through intranasal route with detectable IgA and IgG. Therefore, implication of the platform as a potential vaccine candidate has potential as a future prophylactic vaccine that guarantees further investigations for the assessment of its immunogenicity in humans.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10155293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of long-term adverse events regarding different COVID-19 vaccine regimens within an 18-month follow-up study. 在18个月的随访研究中评估不同COVID-19疫苗方案的长期不良事件

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad010

Mona Sadat Larijani, Rahim Sorouri, Sana Eybpoosh, Delaram Doroud, Ladan Moradi, Mozhgan Ahmadinezhad, Anahita Bavand, Fatemeh Ashrafian, Parinaz Tajmehrabi Namini, Mahsan Zali, Amitis Ramezani

{"title":"Assessment of long-term adverse events regarding different COVID-19 vaccine regimens within an 18-month follow-up study.","authors":"Mona Sadat Larijani, Rahim Sorouri, Sana Eybpoosh, Delaram Doroud, Ladan Moradi, Mozhgan Ahmadinezhad, Anahita Bavand, Fatemeh Ashrafian, Parinaz Tajmehrabi Namini, Mahsan Zali, Amitis Ramezani","doi":"10.1093/femspd/ftad010","DOIUrl":"https://doi.org/10.1093/femspd/ftad010","url":null,"abstract":"Early reports on coronavirus disease 2019 (COVID-19) vaccines presented the short-term adverse events (AEs). This follow-up study investigated a standard regimen based on protein subunit vaccines, PastoCovac and PastoCovac Plus, and the combinational vaccine regimens including AstraZeneca/PastoCovac Plus and Sinopharm/PastoCovac Plus. The participants were followed up to 6 months post the booster shot. All the AEs were collected through in-depth interviews using a valid researcher-made questionnaire and were evaluated regarding the association with the vaccines. Of the 509 individuals, 6.2% of the combinational vaccine participants had late AEs, of whom 3.3% suffered from cutaneous manifestations, followed by 1.1% arthralgia complaints, 1.1% with neurologic disorders, 0.3% ocular problems and 0.3% metabolic complications, with no significant difference between the vaccine regimens. For the standard regimen, 2% of the individuals experienced late AEs as (1%), neurological disorders (0.3%), metabolic problems (0.3%) and involvement of joints (0.3%). Notably, 75% of the AEs were persistent up to the end of the study. A low number of late AEs were captured in 18 months as 12 improbable, 5 unclassifiable, 4 possible and 3 probable associated AEs with the vaccine regimens. The benefits of COVID-19 vaccination far exceed the potential risks and late AEs seem to be uncommon.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Role of Hypoxia-inducible factor 1α in host defense during pneumococcal pneumonia. 缺氧诱导因子1α在肺炎球菌肺炎中宿主防御中的作用。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftac047

Liza Pereverzeva, Natasja A Otto, Hessel Peters-Sengers, Joris J T H Roelofs, Alex F de Vos, Tom van der Poll

{"title":"Role of Hypoxia-inducible factor 1α in host defense during pneumococcal pneumonia.","authors":"Liza Pereverzeva, Natasja A Otto, Hessel Peters-Sengers, Joris J T H Roelofs, Alex F de Vos, Tom van der Poll","doi":"10.1093/femspd/ftac047","DOIUrl":"https://doi.org/10.1093/femspd/ftac047","url":null,"abstract":"Hypoxia-inducible factor (HIF)1α is a transcription factor involved in cellular metabolism and regulation of immune cell effector functions. Here, we studied the role of HIF1α in myeloid cells during pneumonia caused by the major causative pathogen, Streptococcus pneumoniae (Spneu). Mice deficient for HIF1α in myeloid cells (LysMcreHif1αfl/fl) were generated to study the in vitro responsiveness of bone marrow-derived macrophages (BMDMs) and alveolar macrophages (AMs) to the Gram-positive bacterial wall component lipoteichoic acid (LTA) and heat-killed Spneu, and the in vivo host response after infection with Spneu via the airways. Both BMDMs and AMs released more lactate upon stimulation with LTA or Spneu, indicative of enhanced glycolysis; HIF1α-deficiency in these cells was associated with diminished lactate release. In BMDMs, HIF1α-deficiency resulted in reduced secretion of tumor necrosis factor (TNF)α and interleukin (IL)-6 upon activation with Spneu but not LTA, while HIF1α-deficient AMs secreted less TNFα and IL-6 in response to LTA, and TNFα after Spneu stimulation. However, no difference was found in the host response of LysMcreHif1αfl/fl mice after Spneu infection as compared to controls. Similar in vivo findings were obtained in neutrophil (Mrp8creHif1αfl/fl) HIF1α-deficient mice. These data suggest that myeloid HIF1α is dispensable for the host defense during pneumococcal pneumonia.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10817697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First molecular detection of Entamoeba gingivalis subtypes in individuals from Turkey. 首次在土耳其个体中进行齿龈内阿米巴亚型的分子检测。

IF 3.3 4区医学

Pathogens and disease Pub Date : 2023-01-17 DOI: 10.1093/femspd/ftad017

Serra Örsten, Cem Şahin, Engin Yılmaz, Yakut Akyön

{"title":"First molecular detection of Entamoeba gingivalis subtypes in individuals from Turkey.","authors":"Serra Örsten, Cem Şahin, Engin Yılmaz, Yakut Akyön","doi":"10.1093/femspd/ftad017","DOIUrl":"10.1093/femspd/ftad017","url":null,"abstract":"Entamoeba gingivalis is a parasitic protozoan that colonizes the human oral cavity and there are two subtypes (ST1 and ST2) that have been identified to date. However, there are no reports on the molecular detection or characterization of E. gingivalis in Turkey. The objective of this study was to detect the presence of E. gingivalis in Turkish healthy individuals and those with periodontal disease and to subtype the isolates using molecular techniques. Samples from the oral cavity of 94 individuals were taken and the presence of E. gingivalis was determined by PCR using primers for SsrRNA and the amplicons were then confirmed by DNA sequencing. Each participant completed a questionnaire that included demographic data, habits and lifestyle, as well as health status. The presence of E. gingivalis was detected in a total of 19 samples (11 patients and eight healthy individuals). Molecular characterization determined that 12 samples belonged to ST1 and seven samples belonged to ST2. The presence of E. gingivalis was higher in patients with periodontal disease than in healthy individuals, and this association was statistically significant (P < .05). This study constitutes the first report of molecular detection and subtyping of E. gingivalis in Turkey.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":"81 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10553396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10275595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0