Pathogens最新文献_第7页

The Use of Dried Matrix Spots as an Alternative Sampling Technique for Monitoring Neglected Tropical Diseases 使用干基质斑点作为监测被忽视热带病的替代采样技术

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090734

Wanesa Richert, Krzysztof Korzeniewski

引用次数: 0

Susceptibility of Oilseed Radish (Raphanus sativus subsp. oleiferus) Cultivars and Various Brassica Crops to Plasmodiophora brassicae 油籽萝卜（Raphanus sativus subsp.

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090739

Ann-Charlotte Wallenhammar, Eva Edin, Anders Jonsson

{"title":"Susceptibility of Oilseed Radish (Raphanus sativus subsp. oleiferus) Cultivars and Various Brassica Crops to Plasmodiophora brassicae","authors":"Ann-Charlotte Wallenhammar, Eva Edin, Anders Jonsson","doi":"10.3390/pathogens13090739","DOIUrl":"https://doi.org/10.3390/pathogens13090739","url":null,"abstract":"Oilseed radish (OR; Raphanus sativus var. oleiferus) is grown as a cover crop and develops a unique taproot, absorbing nitrogen left by the previous crop. The aim of this project was to investigate the resistance of OR cultivars (cvs.) to Plasmodiophora brassicae, the causal agent of clubroot disease. Twelve market cvs. were compared with cvs. of clubroot-resistant (CR) winter oilseed rape (OSR; Brassica napus) and other selected species of the Brassicaceae family. The study was performed as a replicated bioassay in a growth chamber using a specially composed mixture of field soils holding the natural inoculum of P. brassicae. The results show that the OR cultivars were infected, which implies that OR multiplies the pathogen. The susceptibility of the OR cultivars was not significantly different from that of the CR OSR cultivars Alister and Archimedes, but it was significantly different from that of the OSR cv. Mendel. The disease severity index (DSI) for OR cultivars ranged from 2.3 to 9.3, and disease incidence was 3–17%. The best performance was shown by black radish (Raphanus sativus var. niger) with a DSI of 0.3. For sustainable brassica crop production, we suggest avoiding OR as a cover crop in crop rotations, including OSR or other brassica crops, since there is a risk of increasing inoculum in the soil.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Botanical Extracts with Repellent and Insecticidal Activity and Their Suitability for Managing Mosquito-Borne Disease Risk in Mexico 具有驱蚊和杀虫活性的植物提取物及其在管理墨西哥蚊媒疾病风险方面的适用性综述

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090737

Josselin Carolina Corzo-Gómez, Josué Vidal Espinosa-Juárez, Jose Carlos Ovando-Zambrano, Alfredo Briones-Aranda, Abumalé Cruz-Salomón, Héctor Armando Esquinca-Avilés

{"title":"A Review of Botanical Extracts with Repellent and Insecticidal Activity and Their Suitability for Managing Mosquito-Borne Disease Risk in Mexico","authors":"Josselin Carolina Corzo-Gómez, Josué Vidal Espinosa-Juárez, Jose Carlos Ovando-Zambrano, Alfredo Briones-Aranda, Abumalé Cruz-Salomón, Héctor Armando Esquinca-Avilés","doi":"10.3390/pathogens13090737","DOIUrl":"https://doi.org/10.3390/pathogens13090737","url":null,"abstract":"Among the main arboviruses affecting public health in tropical regions are dengue, zika, and chikungunya, transmitted mainly by mosquitoes of the Aedes genus, especially Aedes aegypti. In recent years, outbreaks have posed major challenges to global health, highlighting the need for integrated and innovative strategies for their control and prevention. Prevention strategies include the elimination of vectors and avoiding mosquito bites; this can be achieved through the use of bioinsecticides and repellents based on plant phytochemicals, as they offer sustainable, ecological, and low-cost alternatives. Mexico has a variety of plants from which both extracts and essential oils have been obtained which have demonstrated significant efficacy in repelling and/or killing insect vectors. This review examines the current knowledge on plant species found in Mexico which are promising options concerning synthetic compounds in terms of their repellent and insecticidal properties against mosquitoes of the genus Aedes and that are friendly to the environment and health.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis 毛滴虫病毒不同种类对药物的敏感性差异允许产生不同的阴道毛滴虫同源克隆

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090733

Carrie A. Hetzel, Akua A. Appah-Sampong, Austin R. Hurst-Manny, Max L. Nibert

{"title":"Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis","authors":"Carrie A. Hetzel, Akua A. Appah-Sampong, Austin R. Hurst-Manny, Max L. Nibert","doi":"10.3390/pathogens13090733","DOIUrl":"https://doi.org/10.3390/pathogens13090733","url":null,"abstract":"Trichomonas vaginalis (Tvag) is a sexually transmitted human pathogen that is commonly infected with strains of one or more of five known species of Trichomonas vaginalis viruses (TVVs), members of genus Trichomonasvirus. TVVs are thought not to have an extracellular phase to their lifecycle and instead to be transmitted vertically from mother to daughter cells. As a result, generation of isogenic virus-positive and virus-negative sets of Tvag clones has been a major barrier to studying interactions between TVVs and their host. Nucleoside analog 2′-C-methylcytidine (2CMC) has been recently reported to clear trichomonads of infections with TVV1, TVV2, and TVV3. We used 2CMC to treat a panel of Tvag isolates that collectively harbor at least one representative strain of each TVV species and thereby provided evidence that infections with TVV4 and TVV5 can also be cleared by 2CMC. Furthermore, our results suggest a newly identified difference in drug susceptibility between TVV species. We took advantage of these susceptibility difference to generate isogenic sets of Tvag clones harboring different combinations of the five TVV species. These results provide both new insight into differences between these species and new avenues for generating tools to study the potential roles of TVVs in Tvag biology.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for Horizontal Transmission and Recirculation of Shiga Toxin-Producing Escherichia coli in the Beef Production Chain in South Africa Using Whole Genome Sequencing 利用全基因组测序证明产志贺毒素大肠杆菌在南非牛肉生产链中的水平传播和再循环

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090732

Libby Obumneke Onyeka, Abiodun A. Adesiyun, Arshad Ismail, Mushal Allam, Karen H. Keddy, Peter N. Thompson

{"title":"Evidence for Horizontal Transmission and Recirculation of Shiga Toxin-Producing Escherichia coli in the Beef Production Chain in South Africa Using Whole Genome Sequencing","authors":"Libby Obumneke Onyeka, Abiodun A. Adesiyun, Arshad Ismail, Mushal Allam, Karen H. Keddy, Peter N. Thompson","doi":"10.3390/pathogens13090732","DOIUrl":"https://doi.org/10.3390/pathogens13090732","url":null,"abstract":"We used whole genome sequencing (WGS) as an epidemiologic surveillance tool to elucidate the transmission dynamics of Shiga toxin-producing Escherichia coli (STEC) strains along the beef production chain in South Africa. Isolates were obtained from a cattle farm, abattoirs and retail outlets. Isolates were analysed using WGS on a MiSeq platform (Illumina, San Diego, CA, USA) and phylogenetic analysis was carried out. Of the 85 isolates, 39% (33) carried the stx gene and 61% (52) had lost the stx gene. The prevalence of stx subtypes was as follows; stx1a 55% (18/33), stx1b 52% (17/33), stx2a 55% (18/33), stx2b 27% (9/33), stx2dB 30% (10/33) and stx2d1A 15% (5/33). Thirty-five different serogenotypes were detected, of which 65% (56) were flagellar H-antigens and 34% (29) were both O-antigens and flagellar H-antigens. We identified 50 different sequence types (STs), and only nine of the isolates were assigned to three different clonal complexes. Core genome phylogenetic analysis revealed genetic relatedness, and isolates clustered mainly according to their STs and serogenotypes regardless of stx subtypes. This study provides evidence of horizontal transmission and recirculation of STEC strains in Gauteng province and demonstrates that every stage of the beef production chain plays a significant role in STEC entry into the food chain.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Specific PCR Assay for Theileria sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle 开发横山毛癣菌特异性 PCR 检测方法并评估其导致牛贫血的可能性

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090735

Iromy Dhananjani Amarasiri, Kalaichelvan Nizanantha, Ngigi Noel Muthoni Mumbi, Isuru Sachintha Kothalawala, Sampath Madusanka, Wettam Perumage Pavithra Sandamali Indrasiri Perera, Hemal Kothalawala, Thillaiampalam Sivakumar, Naoaki Yokoyama

{"title":"Development of a Specific PCR Assay for Theileria sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle","authors":"Iromy Dhananjani Amarasiri, Kalaichelvan Nizanantha, Ngigi Noel Muthoni Mumbi, Isuru Sachintha Kothalawala, Sampath Madusanka, Wettam Perumage Pavithra Sandamali Indrasiri Perera, Hemal Kothalawala, Thillaiampalam Sivakumar, Naoaki Yokoyama","doi":"10.3390/pathogens13090735","DOIUrl":"https://doi.org/10.3390/pathogens13090735","url":null,"abstract":"The clinical implications of Theileria sp. Yokoyama, a recently identified Theileria species in cattle, remain uncertain. The objective of the present study was to evaluate the anemia status in cattle infected with Theileria sp. Yokoyama. Blood samples were collected from 206 cattle across seven Veterinary Ranges in Sri Lanka and analyzed for red blood cell (RBC) indices, including hemoglobin concentration, hematocrit, and RBC counts. Additionally, DNA was extracted from the samples and screened with a newly developed Theileria sp. Yokoyama-specific PCR assay targeting the cytochrome b gene. The PCR results revealed that 60 (29.1%) of the surveyed cattle tested positive for Theileria sp. Yokoyama, with 47 (78.3%) of them being co-infected with other hemopathogen species. Our findings revealed that the cattle breeds, management systems, and tick infestations are potential risk factors for the Theileria sp. Yokoyama infection. Next, we evaluated the anemia status among the surveyed cattle based on the RBC indices. We found that all non-infected cattle were non-anemic. By contrast, anemia was observed in 15 Theileria sp. Yokoyama-infected cattle, including 3 singly infected (anemia rate 3/13, 23.1%) and 12 co-infected cattle (12/47, 25.5%). Our findings suggest that Theileria sp. Yokoyama causes anemia in infected cattle.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal Modeling of Aedes aegypti Risk: Enhancing Dengue Virus Control through Meteorological and Remote Sensing Data in French Guiana 埃及伊蚊风险的时空建模：通过法属圭亚那的气象和遥感数据加强登革热病毒控制

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-29 DOI: 10.3390/pathogens13090738

Sarah Bailly, Vanessa Machault, Samuel Beneteau, Philippe Palany, Camille Fritzell, Romain Girod, Jean-Pierre Lacaux, Philippe Quénel, Claude Flamand

{"title":"Spatiotemporal Modeling of Aedes aegypti Risk: Enhancing Dengue Virus Control through Meteorological and Remote Sensing Data in French Guiana","authors":"Sarah Bailly, Vanessa Machault, Samuel Beneteau, Philippe Palany, Camille Fritzell, Romain Girod, Jean-Pierre Lacaux, Philippe Quénel, Claude Flamand","doi":"10.3390/pathogens13090738","DOIUrl":"https://doi.org/10.3390/pathogens13090738","url":null,"abstract":"French Guiana lacks a dedicated model for developing an early warning system tailored to its entomological contexts. We employed a spatiotemporal modeling approach to predict the risk of Aedes aegypti larvae presence in local households in French Guiana. The model integrated field data on larvae, environmental data obtained from very high-spatial-resolution Pleiades imagery, and meteorological data collected from September 2011 to February 2013 in an urban area of French Guiana. The identified environmental and meteorological factors were used to generate dynamic maps with high spatial and temporal resolution. The study collected larval data from 261 different surveyed houses, with each house being surveyed between one and three times. Of the observations, 41% were positive for the presence of Aedes aegypti larvae. We modeled the Aedes larvae risk within a radius of 50 to 200 m around houses using six explanatory variables and extrapolated the findings to other urban municipalities during the 2020 dengue epidemic in French Guiana. This study highlights the potential of spatiotemporal modeling approaches to predict and monitor the evolution of vector-borne disease transmission risk, representing a major opportunity to monitor the evolution of vector risk and provide valuable information for public health authorities.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR Detection of Bartonella spp. and Borreliella spp. DNA in Dry Blood Spot Samples from Human Patients PCR 检测人类患者干血斑样本中的巴顿氏菌属和鲍瑞利亚氏菌属 DNA

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-28 DOI: 10.3390/pathogens13090727

Kerry L. Clark, Shirley Hartman

{"title":"PCR Detection of Bartonella spp. and Borreliella spp. DNA in Dry Blood Spot Samples from Human Patients","authors":"Kerry L. Clark, Shirley Hartman","doi":"10.3390/pathogens13090727","DOIUrl":"https://doi.org/10.3390/pathogens13090727","url":null,"abstract":"Lyme disease is the most commonly reported vector-borne disease in the United States. Bartonella constitute an additional zoonotic pathogen whose public health impact and diversity continue to emerge. Rapid, sensitive, and specific detection of these and other vector-borne pathogens remains challenging, especially for patients with persistent infections. This report describes an approach for DNA extraction and PCR testing for the detection of Bartonella spp. and Borreliella spp. from dry blood spot (DBS) specimens from human patients. The present study included extraction of DNA and PCR testing of DBS samples from 105 patients with poorly defined, chronic symptoms labeled as Lyme-Like Syndromic Illness (LLSI). Bartonella spp. DNA was detected in 20/105 (19%) and Borreliella spp. DNA was detected in 41/105 (39%) patients with LLSI. Neither group of organisms was detected in DBS samples from 42 healthy control subjects. Bartonella spp. 16S–23S rRNA internal transcribed spacer sequences were highly similar to ones previously identified in yellow flies, lone star ticks, a human patient from Florida, mosquitoes in Europe, or B. apihabitans and choladocola strains from honeybees. These human strains may represent new genetic strains or groups of human pathogenic species of Bartonella. The 41 Borreliella spp. flaB gene sequences obtained from human patients suggested the presence of four different species, including B. burgdorferi, B. americana, B. andersonii, and B. bissettiae/carolinensis-like strains. These results suggest that specific aspects of the DBS DNA extraction and PCR approach enabled the detection of Bartonella spp. and Borreliella spp. DNA from very small amounts of human whole blood from some patients, including specimens stored on filter paper for 17 years.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the Potential: Epstein-Barr Virus (EBV) in Gastric Cancer and Future Treatment Prospects, a Literature Review 释放潜能：胃癌中的 Epstein-Barr 病毒 (EBV) 与未来治疗前景，文献综述

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-28 DOI: 10.3390/pathogens13090728

Salvatore Corallo, Angioletta Lasagna, Beatrice Filippi, Domiziana Alaimo, Anna Tortorella, Francesco Serra, Alessandro Vanoli, Paolo Pedrazzoli

{"title":"Unlocking the Potential: Epstein-Barr Virus (EBV) in Gastric Cancer and Future Treatment Prospects, a Literature Review","authors":"Salvatore Corallo, Angioletta Lasagna, Beatrice Filippi, Domiziana Alaimo, Anna Tortorella, Francesco Serra, Alessandro Vanoli, Paolo Pedrazzoli","doi":"10.3390/pathogens13090728","DOIUrl":"https://doi.org/10.3390/pathogens13090728","url":null,"abstract":"Gastric cancer (GC) is a complex disease with various etiologies. While Helicobacter pylori infection is still one of the leading risk factors for GC, increasing evidence suggests a link between GC and other infective agents such as Epstein Bar Virus (EBV). EBV-associated gastric cancer (EBVaGC) is now recognized as a distinct subgroup of GC, and the complex interactions between the virus and gastric mucosa may influence its development. A recent integrative analysis of the genome and proteome of GC tissues by The Cancer Genome Atlas project has identified EBVaGC as a specific subtype characterized by PIK3CA and ARID1A mutations, extensive DNA hyper-methylation, and activation of immune signaling pathways. These molecular characteristics are markers of the unique molecular profile of this subset of GC and are potential targets for therapy. This review aims to provide an overview of the current knowledge on EBVaGC. It will focus on the epidemiology, clinic-pathological features, and genetic characteristics of EBVaGC. Additionally, it will discuss recent data indicating the potential use of EBV infection as a predictive biomarker of response to chemotherapy and immune checkpoint inhibitors. The review also delves into potential therapeutic approaches for EBVaGC, including targeted therapies and adoptive immunotherapy, highlighting the promising potential of EBV as a therapeutic target.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contributions of Long-Read Sequencing for the Detection of Antimicrobial Resistance 长读测序对检测抗菌药耐药性的贡献

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-28 DOI: 10.3390/pathogens13090730

Roberto Sierra, Mélanie Roch, Milo Moraz, Julien Prados, Nicolas Vuilleumier, Stéphane Emonet, Diego O. Andrey

{"title":"Contributions of Long-Read Sequencing for the Detection of Antimicrobial Resistance","authors":"Roberto Sierra, Mélanie Roch, Milo Moraz, Julien Prados, Nicolas Vuilleumier, Stéphane Emonet, Diego O. Andrey","doi":"10.3390/pathogens13090730","DOIUrl":"https://doi.org/10.3390/pathogens13090730","url":null,"abstract":"Background. In the context of increasing antimicrobial resistance (AMR), whole-genome sequencing (WGS) of bacteria is considered a highly accurate and comprehensive surveillance method for detecting and tracking the spread of resistant pathogens. Two primary sequencing technologies exist: short-read sequencing (50–300 base pairs) and long-read sequencing (thousands of base pairs). The former, based on Illumina sequencing platforms (ISPs), provides extensive coverage and high accuracy for detecting single nucleotide polymorphisms (SNPs) and small insertions/deletions, but is limited by its read length. The latter, based on platforms such as Oxford Nanopore Technologies (ONT), enables the assembly of genomes, particularly those with repetitive regions and structural variants, although its accuracy has historically been lower. Results. We performed a head-to-head comparison of these techniques to sequence the K. pneumoniae VS17 isolate, focusing on blaNDM resistance gene alleles in the context of a surveillance program. Discrepancies between the ISP (blaNDM-4 allele identified) and ONT (blaNDM-1 and blaNDM-5 alleles identified) were observed. Conjugation assays and Sanger sequencing, used as the gold standard, confirmed the validity of ONT results. This study demonstrates the importance of long-read or hybrid assemblies for accurate carbapenemase resistance gene identification and highlights the limitations of short reads in the context of gene duplications or multiple alleles. Conclusions. In this proof-of-concept study, we conclude that recent long-read sequencing technology may outperform standard short-read sequencing for the accurate identification of carbapenemase alleles. Such information is crucial given the rising prevalence of strains producing multiple carbapenemases, especially as WGS is increasingly used for epidemiological surveillance and infection control.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0