Pathologie-biologie最新文献_第9页

Impact des anticorps anti-HLA sur le devenir de l’allogreffe de cellules souches hématopoïétiques : un rapport par la SFGM-TC 抗hla抗体对造血干细胞同种异体移植命运的影响:SFGM-TC的一份报告

Pathologie-biologie Pub Date : 2014-08-01 DOI: 10.1016/j.patbio.2014.05.007

P. Loiseau , V. Dubois , B. Bonafoux , C.-E. Bulabois , V. Coiteux , J.-F. Eliaou , M. Labaky , M. Michallet , V. Renac , F. Delbos , A. Kennel , M. Detrait , A. Devys , C. Galambrun , I. Yakoub-Agha

引用次数: 4

Greffes de cellules souches hématopoïétiques à partir d’un donneur haplo-identique : recommandations de la SFGM-TC (première partie) 单倍相同供体造血干细胞移植:SFGM-TC的建议(第一部分)

Pathologie-biologie Pub Date : 2014-08-01 DOI: 10.1016/j.patbio.2014.05.004

D. Blaise , S. Nguyen , J.-O. Bay , P. Chevallier , N. Contentin , N. Dhédin , R. Duléry , J.-F. Eliaou , M.-T. Rubio , F. Suarez , C.-E. Bulabois , J. Cornillon , A. Huynh , L. Magro , M. Michallet , C. Paillard , P. Turlure , I. Yakoub-Agha

引用次数: 13

Quality controls on cord blood unit contiguous segments: Recommendation of the SFGM-TC 脐带血单位连续段的质量控制:SFGM-TC的建议

Pathologie-biologie Pub Date : 2014-08-01 DOI: 10.1016/j.patbio.2014.05.005

J. De Vos , B. Birebent , C. Faucher , O. Giet , Y. Hicheri , C. Lemarie , E. Baudoux , F. Boulanger , F. Garnier , J. Larghero , F. Pouthier , J.-B. Thibert , I. Yakoub-Agha

引用次数: 11

Immunosuppresseurs dans la prévention de la réaction du greffon contre l’hôte : rapport de la SFGM-TC 免疫抑制剂在预防移植物对宿主反应中的作用:SFGM-TC报告

Pathologie-biologie Pub Date : 2014-08-01 DOI: 10.1016/j.patbio.2014.05.010

S. Belaiche , N. Yafour , S. Balcaen , Y. Beguin , C. Borel , B. Bruno , S. Godin , H. Labussiere-Wallet , N. Sanhamut , A. Charbonnier , E. de Berranger , J. Konopacki-Potet , P. Turlure , I. Yakoub-Agha

引用次数: 7

Autogreffe des cellules souches hématopoïétiques dans les maladies auto-immunes : recommandations de la SFGM-TC 造血干细胞在自身免疫性疾病中的自体移植:SFGM-TC的建议

Pathologie-biologie Pub Date : 2014-08-01 DOI: 10.1016/j.patbio.2014.05.012

D. Farge , L. Terriou , M. Badoglio , A. Cras , P. Desreumaux , S. Hadj-Khelifa , Z. Marjanovic , A. Moisan , R. Dulery , C. Faucher , A. Hij , T. Martin , P. Vermersch , I. Yakoub-Agha

引用次数: 6

Quatrième ateliers de la SFGM-TC pour l’harmonisation des pratiques en allogreffe 第四次SFGM-TC异体移植实践协调研讨会

Pathologie-biologie Pub Date : 2014-08-01 DOI: 10.1016/j.patbio.2014.05.001

I. Yakoub-Agha (Président de la SFGM-TC)

引用次数: 3

Control of an outbreak due to orthopedic infections caused by Enterobacteriaceae producing IMP-4 or IMP-8 carbapenemases 控制由产生IMP-4或IMP-8碳青霉烯酶的肠杆菌科引起的骨科感染暴发

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.01.004

F. Pang , X.-Q. Jia , B. Wang , Y.-H. Li , Q.-G. Zhao

{"title":"Control of an outbreak due to orthopedic infections caused by Enterobacteriaceae producing IMP-4 or IMP-8 carbapenemases","authors":"F. Pang , X.-Q. Jia , B. Wang , Y.-H. Li , Q.-G. Zhao","doi":"10.1016/j.patbio.2014.01.004","DOIUrl":"10.1016/j.patbio.2014.01.004","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate control of an outbreak due to orthopedic infections caused by Enterobacteriaceae producing IMP carbapenemases.</p></div><div><h3>Methods</h3><p>The sporadic orthopedic infections with Enterobacteriaceae producing carbapenemase (CPE) were retrospectively analyzed in a Chinese tertiary care hospital from November 2010 to September 2012.</p></div><div><h3>Results</h3><p>The CPE were isolated from four distinct orthopedic patients, three patients infected with <em>Enterobacter cloacae</em> while the other with <em>Klebsiella oxytoca</em>. All strains were resistant to almost all the conventional antimicrobial. The strains produced IMP-4 type detected in the two early patients, while other strains could produce IMP-8 type. All of the four patients had ever undergoing invasive surgical procedure, and three of them were given fluoroquinolones for anti-infection treatment while the other patients was treated with meropenem. Ultimately, all patients were cured and discharged, without outbreak of nosocomial infection caused by CPE.</p></div><div><h3>Conclusion</h3><p>Our study shows that strict infection control plays an important role in limiting dissemination of Enterobacteriaceae producing IMP carbapenemase. In addition, reasonable supporting treatment and disinfection protection seems to be more effective for the infection of strains.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32222106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

PSA-PSMA profiles and their impact on sera PSA levels and angiogenic activity in hyperplasia and human prostate cancer 前列腺增生和前列腺癌患者血清PSA水平和血管生成活性的影响

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.02.012

A. Ben Jemaa , Y. Bouraoui , S. Sallami , A. Banasr , Y. Nouira , R. Oueslati

{"title":"PSA-PSMA profiles and their impact on sera PSA levels and angiogenic activity in hyperplasia and human prostate cancer","authors":"A. Ben Jemaa , Y. Bouraoui , S. Sallami , A. Banasr , Y. Nouira , R. Oueslati","doi":"10.1016/j.patbio.2014.02.012","DOIUrl":"10.1016/j.patbio.2014.02.012","url":null,"abstract":"<div><h3>Aim</h3><p>The relevance of prostate specific antigen (PSA)-prostate specific membrane antigen (PSMA) profiles in pathologic prostate (hyperplasia and cancer) has not been fully understood. The aim of this study is to investigate the impact of PSA-PSMA profiles on sera PSA levels and angiogenic activity in benign prostate hyperplasia (BPH) and prostate carcinoma (PC).</p></div><div><h3>Patients and methods</h3><p>The study has been carried out in 6 normal prostate (NP), 29 BPH and 33 PC with dominant Gleason grade>8. Immunohistochemical analysis has been performed. Monoclonal antibodies 3E6 and ER-PR8 have been used to assess PSMA and PSA expression respectively. The evaluation of angiogenesis has been made by CD34 immune marker. Serum levels of PSA have been assayed by Immulite autoanalyser.</p></div><div><h3>Results</h3><p>The study of each protein separately among sera PSA levels showed that PSMA expression and angiogenic activity have the highest intensity in PC patients with serum PSA levels>20ng/mL. Nevertheless, the lowest tissue PSA expression was found in PC patients with this latter sera PSA group. The most relevant results showed that in PC patients (PSA+, PSMA+) and (PSA–, PSMA+) profile were found to be inversely related to sera PSA levels. In PC patients, a high immunoexpression of (PSA+, PSMA+) profile has detected in the sera PSA group>20ng/mL; whereas a high immunoexpression of (PSA–, PSMA+) profile was detected in the sera PSA group between 0 and 4ng/mL. The highest angiogenic activity was found in PC patients with (PSA+, PSMA+) profile.</p></div><div><h3>Conclusions</h3><p>Our findings clearly have supported the feasibility of PSA-PSMA profiles to improve in vivo diagnostic and therapeutic approaches in prostate cancer patients.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.02.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32273890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Protein aggregation and prionopathies 蛋白质聚集和朊病毒病

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.01.003

M. Renner , R. Melki

{"title":"Protein aggregation and prionopathies","authors":"M. Renner , R. Melki","doi":"10.1016/j.patbio.2014.01.003","DOIUrl":"10.1016/j.patbio.2014.01.003","url":null,"abstract":"<div><p>Prion protein and prion-like proteins share a number of characteristics. From the molecular point of view, they are constitutive proteins that aggregate following conformational changes into insoluble particles. These particles escape the cellular clearance machinery and amplify by recruiting the soluble for of their constituting proteins. The resulting protein aggregates are responsible for a number of neurodegenerative diseases such as Creutzfeldt-Jacob, Alzheimer, Parkinson and Huntington diseases. In addition, there are increasing evidences supporting the inter-cellular trafficking of these aggregates, meaning that they are “transmissible” between cells. There are also evidences that brain homogenates from individuals developing Alzheimer and Parkinson diseases propagate the disease in recipient model animals in a manner similar to brain extracts of patients developing Creutzfeldt-Jacob's disease. Thus, the propagation of protein aggregates from cell to cell may be a generic phenomenon that contributes to the evolution of neurodegenerative diseases, which has important consequences on human health issues. Moreover, although the distribution of protein aggregates is characteristic for each disease, new evidences indicate the possibility of overlaps and crosstalk between the different disorders. Despite the increasing evidences that support prion or prion-like propagation of protein aggregates, there are many unanswered questions regarding the mechanisms of toxicity and this is a field of intensive research nowadays.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.01.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32232044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Reconstruction du cartilage nasal par ingénierie tissulaire à base de polyéthylène de haute densité et d’un hydrogel 以高密度聚乙烯和水凝胶为基础的组织工程鼻软骨重建

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.03.001

M. Durbec , N. Mayer , D. Vertu-Ciolino , F. Disant , F. Mallein-Gerin , E. Perrier-Groult

{"title":"Reconstruction du cartilage nasal par ingénierie tissulaire à base de polyéthylène de haute densité et d’un hydrogel","authors":"M. Durbec , N. Mayer , D. Vertu-Ciolino , F. Disant , F. Mallein-Gerin , E. Perrier-Groult","doi":"10.1016/j.patbio.2014.03.001","DOIUrl":"10.1016/j.patbio.2014.03.001","url":null,"abstract":"<div><h3>Aim of the study</h3><p>Nasal reconstruction remains a challenge for any surgeon. The surgical indications for nasal reconstruction after oncologic resection, trauma or as part of cosmetic rhinoplasty, are steadily increasing. The current attitude for reconstruction is the use of autologous cartilage grafts of various origins (septal, ear or rib) trying to restore a physiological anatomy but their quantity is limited. Thus, in order to produce an implantable cartilaginous model, we developed a study protocol involving human nasal chondrocytes, growth factors and a composite biomaterial and studied at the molecular, cellular and tissue level the phenotype of the chondrocytes cultured in this model.</p></div><div><h3>Materials and methods</h3><p>After extraction of chondrocytes and their amplification on plastic, the cells were cultured for 15days either in monolayer or within an agarose hydrogel or a composite biomaterial (agarose/high density polyethylene: Medpor<sup>®</sup>) in the presence or not of a cocktail of soluble factors (BIT): bone morphogenetic protein-2 (BMP-2), insulin and triiodothyronine (T3). The quality of the chondrocyte phenotype was analyzed by PCR, western blotting and immunohistochemistry.</p></div><div><h3>Results</h3><p>During their amplification in monolayer, chondrocytes dedifferentiate. However, our results show that the BIT cocktail induces redifferentiation of chondrocytes cultured in agarose/Medpor with synthesis of mature chondrogenic markers. Thereby, chondrocytes associated with the agarose hydrogel will colonize Medpor and synthesize an extracellular matrix characteristic of nasal cartilage.</p></div><div><h3>Conclusion</h3><p>This nasal cartilage tissue engineering protocol provides the first interesting results for nasal reconstruction.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32273473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7