Pathologie-biologie最新文献_第10页

Diagnosis and management of nocardiosis after bone marrow stem cell transplantation in adults: Lack of lymphocyte recovery as a major contributing factor 成人骨髓干细胞移植后诺卡菌病的诊断和治疗:缺乏淋巴细胞恢复是一个主要因素

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.04.001

L. Mansi , E. Daguindau , P. Saas , F. Pouthier , C. Ferrand , A. Dormoy , I. Patry , F. Garnache , P.-S. Rohrlich , E. Deconinck , F. Larosa

{"title":"Diagnosis and management of nocardiosis after bone marrow stem cell transplantation in adults: Lack of lymphocyte recovery as a major contributing factor","authors":"L. Mansi , E. Daguindau , P. Saas , F. Pouthier , C. Ferrand , A. Dormoy , I. Patry , F. Garnache , P.-S. Rohrlich , E. Deconinck , F. Larosa","doi":"10.1016/j.patbio.2014.04.001","DOIUrl":"10.1016/j.patbio.2014.04.001","url":null,"abstract":"<div><p>Hematopoietic cell transplantation (HCT) is a curative treatment for hematological malignancies. This therapeutic approach is associated with a profound immune deficiency and an increased rate of opportunistic infections. Nocardiosis is a rare bacterial infection occurring mainly in patients with deficient cell-mediated immunity, such as AIDS patients or transplant recipients. Diagnosis of nocardiosis can be challenging, as signs and symptoms are non-specific. Routine prophylaxis with trimethoprin/sulfamethoxazole (TMP/SMZ) does not prevent the risk of infection. Between May 2001 and December 2009, five cases of nocardiosis were diagnosed from the 366 allogeneic HCT recipients in our centre. Four patients developed a disseminated nocardiosis within the first year after HCT. The fifth patient presented a localized cutaneous nocardiosis. In disseminated cases, median total CD4+ T-cells were below 100cells/μL. Naive CD4+ CD45RA+/RO− T-cells were almost undetectable. CD8<sup>+</sup> T-cells and NK cells were below the normal range and CD19+ B-cell reconstitution was completely deficient. In a localized case, we observed a lack of naive thymic emigrants CD4+ CD45RA+/RO− T-cells.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32380803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Sequence and structural analyses of NSP4 proteins from human group A rotavirus strains detected in Tunisia 突尼斯人A组轮状病毒毒株NSP4蛋白序列和结构分析

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2013.10.006

M. Ben Hadj Fredj , M. Ben Hamida-Rebaï , M. Zeller , E. Heylen , M. Van Ranst , J. Matthijnssens , A. Trabelsi

{"title":"Sequence and structural analyses of NSP4 proteins from human group A rotavirus strains detected in Tunisia","authors":"M. Ben Hadj Fredj , M. Ben Hamida-Rebaï , M. Zeller , E. Heylen , M. Van Ranst , J. Matthijnssens , A. Trabelsi","doi":"10.1016/j.patbio.2013.10.006","DOIUrl":"10.1016/j.patbio.2013.10.006","url":null,"abstract":"<div><h3>Background</h3><p>The NSP4 protein of group A rotavirus (RVA) has been recognized as a viral enterotoxin and plays important roles in viral pathogenesis and morphogenesis. Domains involved in structural and functional interactions have been proposed mainly based on the simian SA11 strain.</p></div><div><h3>Methods</h3><p>NSP4 has been classified into 15 different genotypes (E1-E15), and the aim of this study was to analyze the sequences of 46 RVA strains in order to determine the aminoacid (aa) differences between E1 and E2 genotypes. Another aspect was to characterize the structural and physicochemical properties of these strains.</p></div><div><h3>Results</h3><p>Comparison of deduced aa sequences of the NSP4 protein showed that divergences between NSP4 genotypes E1 and E2 were mostly observed in the VP4-binding, the interspecies variable domain (ISVD) and the double-layered particle (DLP) binding domains. Interestingly, uncommon variations in residues 131 and 138, which are known to be important aa in pathogenesis, were found in one unusual animal derived strain belonging to the E2 genotype. Concerning the structural aspect, no significant differences were noted.</p></div><div><h3>Conclusion</h3><p>The presence of punctual aa variations in the NSP4 genotypes may indicate that NSP4 mutates mainly via accumulation of point mutations.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2013.10.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32216987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Impact de la composition du greffon sur le devenir des patients après une allogreffe de cellules souches hématopoïétiques : corrélation entre proportion des lymphocytes T CD4+ du greffon exprimant le CCR7 et la survenue d’une GVH aiguë 移植物成分对造血干细胞移植后患者预后的影响:表达CCR7的移植物中CD4+ T淋巴细胞比例与急性GVH发生的相关性

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.02.013

B. Choufi , S. Thiant , J. Trauet , M. Cliquennois , M. Cherrel , F. Boulanger , V. Coiteux , L. Magro , M. Labalette , I. Yakoub-Agha

{"title":"Impact de la composition du greffon sur le devenir des patients après une allogreffe de cellules souches hématopoïétiques : corrélation entre proportion des lymphocytes T CD4+ du greffon exprimant le CCR7 et la survenue d’une GVH aiguë","authors":"B. Choufi , S. Thiant , J. Trauet , M. Cliquennois , M. Cherrel , F. Boulanger , V. Coiteux , L. Magro , M. Labalette , I. Yakoub-Agha","doi":"10.1016/j.patbio.2014.02.013","DOIUrl":"10.1016/j.patbio.2014.02.013","url":null,"abstract":"<div><p>In a previous prospective study on 62 patients who underwent an HLA-matched allogeneic stem cell transplantation, we have observed that proportion of donor-derived CCR7<sup>+</sup>/CD4<sup>+</sup> T cells in the graft provided a predictive indicator of acute GVHD without interfering on chronic GVHD and relapse rate. Here we present our results on a confirmatory cohort of 137 consecutive patients. Indeed patients who received more than 76% of CCR7<sup>+</sup>/CD4<sup>+</sup> T cells in the graft developed more often acute GVHD be it of low or high grade than those who did not. Determination of the CCR7<sup>+</sup>/CCR7<sup>neg</sup> ratio of CD4<sup>+</sup> T cells in the graft provides a predictive indicator of acute GVHD and could help to define strategies of partial selective T cell depleted transplantation.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.02.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32406469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Actualité de la résistance aux antibiotiques chez les bacilles à Gram négatif en Algérie 阿尔及利亚革兰氏阴性杆菌抗生素耐药性的新闻

Pathologie-biologie Pub Date : 2014-06-01 DOI: 10.1016/j.patbio.2014.01.005

Z. Baba Ahmed-Kazi Tani , G. Arlet

{"title":"Actualité de la résistance aux antibiotiques chez les bacilles à Gram négatif en Algérie","authors":"Z. Baba Ahmed-Kazi Tani , G. Arlet","doi":"10.1016/j.patbio.2014.01.005","DOIUrl":"10.1016/j.patbio.2014.01.005","url":null,"abstract":"<div><p>Antibiotic resistance has become a major public health problem in Algeria. Indeed the past decade, we have seen a significant increase in resistance to antibiotics especially in Gram-negative bacilli. Resistance to β-lactams in enterobacteria is dominated by the production of ESBL CTX-M-3 and CTX-M-15. The strains producing these enzymes are often the cause of potentially serious infections in both hospital and community settings. Identified plasmid cephalosporinases are CMY-2, CMY-12 and DHA-1. The isolation of strains of Enterobacteriaceae and <em>Pseudomonas aeruginosa</em> producing carbapenemases is rare in Algeria. Some Enterobacteriaceae producing OXA-48 or VIM-19 have been reported; so far, only VIM-2 has been identified in <em>P. aeruginosa</em>. However, the situation regarding the strains of <em>Acinetobacter baumannii</em> resistant to carbapenemases seems to be more disturbing. The carbapenemase OXA-23 is the most common and seems to be endemic in the north. The carbapenemase NDM-1 has also been identified. Resistance to aminoglycosides is marked by the identification <em>armA</em> gene associated with <em>bla</em><sub>CTX-M</sub> genes in strains of <em>Salmonella</em> sp. Several other resistance genes have been identified sporadically in strains of Enterobacteriaceae, <em>P. aeruginosa</em> and <em>A. baumannii</em>. Resistance genes to fluoroquinolones are more recent identification in Algeria. The most common are the Qnr determinants followed by the bifunctional enzyme AAC[6’]-Ib-cr. Resistance to sulfonamides and trimethoprim was also reported in Enterobacteriaceae strains in the west of the country.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.01.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32334128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Prions: A model of conformational disease? 朊病毒:构象疾病的模型?

Pathologie-biologie Pub Date : 2014-04-01 DOI: 10.1016/j.patbio.2014.02.003

F. Morinet

{"title":"Prions: A model of conformational disease?","authors":"F. Morinet","doi":"10.1016/j.patbio.2014.02.003","DOIUrl":"10.1016/j.patbio.2014.02.003","url":null,"abstract":"<div><p>The discovery that a protein could mimic viral and bacterial pathogens around 1980 by Stanley Prusiner was unexpected. Evidence shows now that Creutzfeldt-Jakob disease and related disorders are caused by prions. Prions and, for example neurodegeneratives diseases, arise from the same general disease mechanism. In each, there is abnormal unfolding and then aggregation of proteins. The protein conformational changes associated with the pathogenesis of protein misfolding disorders produce β sheet rich oligomers that are partially resistant to proteolysis and have a high tendency to form amyloid-like aggregates. It is important to distinguish between prions and amyloids: prions need not to polymerize into amyloid fibrils and can undergo self-propagation as oligomers. The prion diseases are characterized by the conformational conversion of PrP<sup>c</sup> to PrP<sup>sc</sup>, the fundamental even underlying prion diseases. Despite the obvious differences between prions and conventional infectious microorganisms, prions fulfill the Koch's postulates. Meaningful treatments are likely to require cocktails of drugs that interfere with the conversion of precursor into prions and enhance the clearance of prions; such an approach may find application in the more common degenerative diseases.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32196705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Paul Bornstein (1934–2013), a pioneer of matrix biology and pathology 保罗·伯恩斯坦(1934-2013)，基质生物学和病理学的先驱

Pathologie-biologie Pub Date : 2014-04-01 DOI: 10.1016/j.patbio.2014.02.008

J. Labat-Robert, L. Robert

引用次数: 0

Tumors perturbing extracellular matrix biosynthesis. The case of von Recklinghausen's disease 干扰细胞外基质生物合成的肿瘤。von Recklinghausen病的案例

Pathologie-biologie Pub Date : 2014-04-01 DOI: 10.1016/j.patbio.2014.02.010

L. Robert

引用次数: 7

Use of mesenchymal stem cells for cutaneous repair and skin substitute elaboration 间充质干细胞用于皮肤修复和皮肤替代的阐述

Pathologie-biologie Pub Date : 2014-04-01 DOI: 10.1016/j.patbio.2014.01.002

B. Laverdet , L. Micallef , C. Lebreton , J. Mollard , J.-J. Lataillade , B. Coulomb , A. Desmoulière

{"title":"Use of mesenchymal stem cells for cutaneous repair and skin substitute elaboration","authors":"B. Laverdet , L. Micallef , C. Lebreton , J. Mollard , J.-J. Lataillade , B. Coulomb , A. Desmoulière","doi":"10.1016/j.patbio.2014.01.002","DOIUrl":"10.1016/j.patbio.2014.01.002","url":null,"abstract":"<div><p>Human mesenchymal stem cells (MSCs) are a heterogeneous population of fibroblast-like cells, which are present in different locations, including bone marrow, adipose tissue, extra-foetal tissues, gingiva and dermis. MSCs, which present multipotency capacities, important expansive potential and immunotolerance properties, remain an attractive tool for tissue repair and regenerative medicine. Currently, several studies and clinical trials highlight the use of MSCs in cutaneous repair underlining that their effects are essentially due to the numerous factors that they release. MSCs are also used in skin substitute development. In this study, we will first discuss the different sources of MSCs actually available. We will then present results showing that bone marrow-derived MSCs prepared according to Good Manufacturing Practices and included in a dermal equivalent are able to promote appropriate epidermis growth and differentiation. These data demonstrate that bone marrow-derived MSCs represent a satisfactory alternative to dermal fibroblasts in order to develop skin substitute. In addition, MSCs could provide a useful alternative to sustain epidermis development and to promote wound healing.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2014.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32202292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Regulation of LRP-1 expression: Make the point LRP-1表达的调控:说明问题

Pathologie-biologie Pub Date : 2014-04-01 DOI: 10.1016/j.patbio.2014.02.002

H. Emonard, L. Théret, A.H. Bennasroune, S. Dedieu

引用次数: 40

Pathologie Biologie, 60 ans déjà ! 病理学生物学，已经60年了!

Pathologie-biologie Pub Date : 2014-04-01 DOI: 10.1016/j.patbio.2014.02.005

M. Boiron

引用次数: 0