Oral microbiology and immunology最新文献_第9页

Detection of oral bacteria in cardiovascular specimens. 心血管标本口腔细菌的检测。

Oral microbiology and immunology Pub Date : 2009-02-01 DOI: 10.1111/j.1399-302X.2008.00479.x

K. Nakano, H. Nemoto, R. Nomura, H. Inaba, H. Yoshioka, K. Taniguchi, A. Amano, Takashi Ooshima

{"title":"Detection of oral bacteria in cardiovascular specimens.","authors":"K. Nakano, H. Nemoto, R. Nomura, H. Inaba, H. Yoshioka, K. Taniguchi, A. Amano, Takashi Ooshima","doi":"10.1111/j.1399-302X.2008.00479.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00479.x","url":null,"abstract":"BACKGROUND/AIMS\u0000Oral bacteria, including cariogenic and periodontal pathogens, are thought to be etiological factors in the development of cardiovascular diseases. To define this relationship, we analyzed the distribution of oral bacterial species in cardiovascular specimens.\u0000\u0000\u0000METHOD\u0000Following acceptance into the study, 203 consecutive patients were analyzed, from whom 82 aortic valve specimens, 35 mitral valve specimens, and 86 aortic aneurysmal wall specimens, of which 16 contained aneurysmal thrombus tissues, were obtained. In addition, a total of 58 dental plaque specimens were collected from the same group of patients who underwent heart valve replacement or removal of aortic aneurysms. Bacterial DNA was extracted from both cardiovascular tissues and dental plaque in those cases and then species-specific polymerase chain reaction assays were used to analyze the occurrences of six oral streptococcal and six periodontal bacterial species.\u0000\u0000\u0000RESULTS\u0000Streptococcus mutans was the most frequently detected species in the cardiovascular specimens, followed by Aggregatibacter actinomycetemcomitans. As for dental plaque specimens from patients who underwent cardiovascular operations, most of the tested periodontitis-related species as well as oral streptococci were detected at high frequencies. Furthermore, the positive rate of S. mutans in cardiovascular specimens from patients whose dental plaque specimens were also positive for S. mutans was 78%, which was significantly higher than any other tested species when the same analysis was performed.\u0000\u0000\u0000CONCLUSION\u0000Our results suggest that specific oral bacterial species, such as S. mutans and A. actinomycetemcomitans, are related to bacteremia and may be etiologic factors for the development of cardiovascular diseases.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 1 1","pages":"64-8"},"PeriodicalIF":0.0,"publicationDate":"2009-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00479.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 182

Oral candidiasis: a comparison between conventional methods and multiplex polymerase chain reaction for species identification. 口腔念珠菌病:传统方法与多重聚合酶链反应鉴定菌种的比较。

Oral microbiology and immunology Pub Date : 2009-02-01 DOI: 10.1111/j.1399-302X.2008.00447.x

G. Liguori, V. Di Onofrio, A. Lucariello, F. Gallé, G. Signoriello, G. Colella, M. D’Amora, F. Rossano

{"title":"Oral candidiasis: a comparison between conventional methods and multiplex polymerase chain reaction for species identification.","authors":"G. Liguori, V. Di Onofrio, A. Lucariello, F. Gallé, G. Signoriello, G. Colella, M. D’Amora, F. Rossano","doi":"10.1111/j.1399-302X.2008.00447.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00447.x","url":null,"abstract":"BACKGROUND/AIM\u0000Oral candidiasis is the most common fungal infection in dental practice, and is caused by yeasts that are normally present in the endogenous flora.\u0000\u0000\u0000METHODS\u0000To evaluate a rapid diagnostic method for identification of Candida oral isolates, a multiplex polymerase chain reaction (PCR) was carried out on colonies and on oral rinse solutions from 95 subjects with suspected oral candidiasis and results were compared with those from seven commonly used phenotypic identification systems.\u0000\u0000\u0000RESULTS\u0000Between four and nine species were characterized in the samples by the phenotypic methods. PCR identified the same species in 60 (74%) samples from both colony and oral rinse solutions. Statistical analysis, carried out only for the three most frequently isolated species (Candida albicans, Candida glabrata, and Candida tropicalis), showed good concordance in the comparison of multiplex PCR with API 20C AUX and with the Rapid Yeast Identification Panel; conversely, significant differences were registered in the comparison between the molecular method and other phenotypic systems, including four chromogenic media and the automated system Vitek2.\u0000\u0000\u0000DISCUSSION\u0000Multiplex PCR was rapid and effective in the identification of Candida species and allowed the detection of more than one species in the same sample.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 1 1","pages":"76-8"},"PeriodicalIF":0.0,"publicationDate":"2009-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00447.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Zinc and copper play a role in coaggregation inhibiting action of Porphyromonas gingivalis. 锌和铜在牙龈卟啉单胞菌共聚集抑制作用中起作用。

Oral microbiology and immunology Pub Date : 2009-02-01 DOI: 10.1111/j.1399-302X.2008.00476.x

M. Tamura, K. Ochiai

{"title":"Zinc and copper play a role in coaggregation inhibiting action of Porphyromonas gingivalis.","authors":"M. Tamura, K. Ochiai","doi":"10.1111/j.1399-302X.2008.00476.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00476.x","url":null,"abstract":"BACKGROUND/AIM\u0000We investigated the mechanisms of adherence of salivary and serum proteins, which mimic gingival crevicular fluid (GCF), to Porphyromonas gingivalis, and the effects of these adhered proteins on coaggregation and hemagglutination properties.\u0000\u0000\u0000METHODS\u0000The amounts of salivary and serum proteins adhering to P. gingivalis were determined using (3)H-labeled and non-labeled proteins. The coaggregation between P. gingivalis and Streptococcus oralis or Streptococcus gordonii was observed. Hemagglutination was evaluated using sheep erythrocytes. Proteins that interacted with zinc or copper in saliva and serum and on P. gingivalis were examined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.\u0000\u0000\u0000RESULTS\u0000The amount of salivary or serum proteins that adhered to the surface of P. gingivalis strains was increased by cations, especially zinc and copper ions. The pretreatment of bacterial cells with salivary or serum proteins before the assay inhibited coaggregation with gram-positive bacteria and hemagglutination. These phenomena were enhanced by the presence of zinc or copper ions during the pretreatment of P. gingivalis with proteins. We detected protein bands that were related to these cations in saliva and serum and on P. gingivalis.\u0000\u0000\u0000CONCLUSIONS\u0000Our findings suggest that zinc and copper ions markedly enhanced the adhesion and accumulation of salivary and serum proteins on cells of P. gingivalis and inhibited the coaggregation and hemagglutination of P. gingivalis. These cations might be useful for limiting the settlement of P. gingivalis in the gingival sulcus with the goal of preventing periodontal disease.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"157 1","pages":"56-63"},"PeriodicalIF":0.0,"publicationDate":"2009-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00476.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Differential effects of five Aggregatibacter actinomycetemcomitans strains on gingival epithelial cells. 5株放线菌聚集菌对牙龈上皮细胞的差异影响。

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00449.x

T Shimada, N Sugano, R Nishihara, K Suzuki, H Tanaka, K Ito

引用次数: 26

Transcriptional analysis of gtfB, gtfC, and gbpB and their putative response regulators in several isolates of Streptococcus mutans. 几种变形链球菌中gtfB、gtfC和gbpB的转录分析及其可能的应答调节因子。

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00451.x

R N Stipp, R B Gonçalves, J F Höfling, D J Smith, R O Mattos-Graner

{"title":"Transcriptional analysis of gtfB, gtfC, and gbpB and their putative response regulators in several isolates of Streptococcus mutans.","authors":"R N Stipp, R B Gonçalves, J F Höfling, D J Smith, R O Mattos-Graner","doi":"10.1111/j.1399-302X.2008.00451.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00451.x","url":null,"abstract":"Background: Streptococcus mutans, a major dental caries pathogen, expresses several virulence genes that mediate its growth, accumulation on tooth surfaces, and acid-mediated tooth demineralization. GtfB and GtfC catalyze the extracellular synthesis of water-insoluble glucan matrix from sucrose, and are essential for accumulation of bacteria in the dental biofilm. GbpB, an essential protein of S. mutans, might also mediate cell-surface interaction with glucan.Aim/methods: In this study, we determined the transcription levels of gtfB, gtfC, and gbpB, and several putative transcriptional response regulators (rr) at different phases of planktonic growth in 11 S. mutans strains.Results: Activities of gtfB and gtfC were growth-phase dependent and assumed divergent patterns in several strains during specific phases of growth, while gbpB activities appeared to be under modest influence of the growth phase. Transcription patterns of the rr vicR, covR, comE, ciaR, and rr1 were growth-phase dependent and some of these genes were expressed in a highly coordinated way. Each rr, except comE, was expressed by all the strains. Patterns of virulence and regulatory genes were, however, strain-specific.Conclusions: The findings suggest that mechanisms controlling virulence gene expression are variable among genotypes, providing the notion that the genetic diversity of S. mutans may have important implications for understanding mechanisms that regulate the expression of virulence genes in this species.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"466-73"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00451.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Relationship between human cytomegalovirus transcription and symptomatic apical periodontitis in Iran. 伊朗人巨细胞病毒转录与症状性根尖牙周炎的关系

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00471.x

K A Yazdi, M Sabeti, F Jabalameli, M Eman eini, S A Kolahdouzan, J Slots

{"title":"Relationship between human cytomegalovirus transcription and symptomatic apical periodontitis in Iran.","authors":"K A Yazdi, M Sabeti, F Jabalameli, M Eman eini, S A Kolahdouzan, J Slots","doi":"10.1111/j.1399-302X.2008.00471.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00471.x","url":null,"abstract":"Background/aims: Apical periodontitis of endodontic origin may develop as a result of cooperative interactions among herpesviruses, specific pathogenic bacteria and tissue-destructive inflammatory mediators. This study sought to identify the presence of Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) transcripts in symptomatic and asymptomatic periapical lesions of individuals living in Iran.Material and methods: Fifty endodontic patients (28 with symptomatic periapical lesions and 22 with asymptomatic periapical lesions) were included in the study. In each study subject, a microbiological periapical sample was collected using a curette in conjunction with periapical surgery. A reverse transcription-polymerase chain reaction assay was used to identify transcripts of EBV and HCMV.Results: Human cytomegalovirus transcript was detected in 15 of the 28 (53.6%) symptomatic and in six of the 22 (27.3%) asymptomatic periapical study lesions (significant difference between symptomatic and asymptomatic lesions; P = 0.03, chi-square test). Epstein-Barr virus transcript was identified in one symptomatic and in two asymptomatic periapical lesions.Conclusion: This study establishes that HCMV transcription is common in apical periodontitis and is most frequent in symptomatic lesions. The high frequency of active herpesvirus infections in severe apical periodontitis changes the pathogenic paradigm of the disease and may also have preventive and therapeutic implications.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"510-4"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00471.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27821072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Anticaries effect of compounds extracted from Galla chinensis in a multispecies biofilm model. 五倍子提取物在多物种生物膜模型中的抗炎作用。

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00450.x

Q Xie, J Li, X Zhou

{"title":"Anticaries effect of compounds extracted from Galla chinensis in a multispecies biofilm model.","authors":"Q Xie, J Li, X Zhou","doi":"10.1111/j.1399-302X.2008.00450.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00450.x","url":null,"abstract":"Introduction: Galla Chinensis is a leaf gall known to have some antibacterial effects. Using an in vitro biofilm model of dental plaque, the present study aimed to evaluate the anticaries effects of Galla Chinensis and its chemical fractions.Methods: A four-organism bacterial consortium (Streptococcus sanguis, Streptococcus mutans, Actinomyces naeslundii, Lactobacillus rhamnosus) was grown on hydroxyapatite (HA) discs, bovine enamel blocks, and glass surfaces in a continuous culture system and exposed to repeated solution pulses. Galla Chinensis extracts, sucrose solutions, and sodium fluoride solutions were pulsed into different flow cells. The pH value of the planktonic phase in each flow cell was recorded and the bacteria colonizing the biofilm on the HA discs were counted. Enamel blocks were observed using a polarized microscope and lesion depth was evaluated. The biofilm morphology was examined with a fluorescence microscope and the images captured were analyzed on an image analysis system.Results: When Galla Chinensis extract, its chemical fraction, or fluoride was added to the sucrose solution, the planktonic phase pH remained higher than that in the sucrose alone. A lower level of colonization on the HA surface was also observed in the groups to which Galla Chinensis and fluoride were added compared with the control sucrose group, and this was reflected in both the total viable count and the biofilm imaging, which showed fewer cariogenic bacteria and a less compact biofilm, respectively. Enamel demineralization in both the fluoride group and the Galla Chinensis group was significantly less than that in the sucrose group.Conclusions: Galla Chinensis and fluoride may inhibit the cariogenicity of the oral biofilm. Galla Chinensis appears to be a promising source of new agents that may prevent dental caries.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"459-65"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00450.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Treponema denticola does not induce production of common innate immune mediators from primary gingival epithelial cells. 密螺旋体不会诱导原代牙龈上皮细胞产生常见的先天免疫介质。

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00452.x

C A Brissette, T-T T Pham, S R Coats, R P Darveau, S A Lukehart

{"title":"Treponema denticola does not induce production of common innate immune mediators from primary gingival epithelial cells.","authors":"C A Brissette, T-T T Pham, S R Coats, R P Darveau, S A Lukehart","doi":"10.1111/j.1399-302X.2008.00452.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00452.x","url":null,"abstract":"It has been hypothesized that the neutrophil chemoattractant interleukin-8 (IL-8) forms a gradient in the oral cavity, with the highest concentration of IL-8 produced closest to the bacterial biofilm. In periodontitis, this gradient is disrupted, impairing neutrophil chemotaxis to diseased sites. Treponema denticola is prominently associated with periodontal disease, yet little is known about its ability to modulate the production of inflammatory mediators by epithelial cells. Others have shown that dentilisin, the major outer membrane protease of T. denticola, degrades IL-8 in vitro. We now provide evidence that T. denticola also fails to induce IL-8 production from primary gingival epithelial cells (PGEC). The lack of IL-8 production is not explained by IL-8 degradation, because a protease mutant that does not degrade IL-8 does not induce IL-8 production with these stimuli either. The lack of innate immune mediator production may be a more global phenomenon because T. denticola fails to induce IL-6 or intercellular adhesion molecule 1 production from PGEC. T. denticola also fails to induce transcription of IL-8 and human beta-defensin-2 messenger RNA. The lack of immune mediator production is not explained by the failure of T. denticola to interact with Toll-like receptor 2 (TLR-2), as T. denticola stimulates nuclear factor-kappaB nuclear translocation in TLR-2-transfected HEK293 cells. Not only can T. denticola degrade the IL-8 present in the periodontal lesion, but this organism also fails to induce IL-8 production by PGEC. The lack of an epithelial cell response to T. denticola may contribute to the pathogenesis of periodontitis by failing to trigger chemotaxis of neutrophils into the periodontal pocket.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"474-81"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00452.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Biofilm formation and autoinducer-2 signaling in Streptococcus intermedius: role of thermal and pH factors. 中间链球菌生物膜形成和自诱导剂-2信号传导:热和pH因子的作用。

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00460.x

N A A M Ahmed, F C Petersen, A Aa Scheie

{"title":"Biofilm formation and autoinducer-2 signaling in Streptococcus intermedius: role of thermal and pH factors.","authors":"N A A M Ahmed, F C Petersen, A Aa Scheie","doi":"10.1111/j.1399-302X.2008.00460.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00460.x","url":null,"abstract":"Background/aim: Bacteria sense their population density using autoinducer (AI) signaling systems. The AI-2 signal is thought to mediate communication among and within bacterial species. Streptococcus intermedius is a commensal organism frequently associated with periodontitis and purulent infections. We investigated the role of AI-2 signaling in S. intermedius biofilm formation under temperatures and pH levels relevant to human physiology.Methods: Bioluminescence was used to monitor the change in AI-2 levels at various temperatures. Growth and biofilm formation in S. intermedius and its luxS mutant SI006 were measured at 35, 37, 39, and 41 degrees C and in pH ranging from 5.7 to 7.5. To confirm the role of AI-2 signals in biofilm formation, the AI-2 precursor (S)-4,5-dihydroxy-2,3-pentanedione (DPD) was used to complement SI006 biofilm formation.Results: S. intermedius AI-2 signals were detected at all growth temperatures but reached the highest levels at 37 degrees C. SI006 displayed significantly less biofilm formation than S. intermedius wild-type (WT); however, the role of AI-2 on biofilm formation was confined to 37 degrees C. DPD supplementation significantly increased SI006 biofilm formation to the S. intermedius WT level. The role of AI-2 in S. intermedius biofilm formation was not influenced by pH. High temperatures and low pH enhanced biofilm formation in both S. intermedius and its luxS mutant.Conclusions: High temperature and acidic conditions may favor biofilm formation by S. intermedius. The role of AI-2 in biofilm formation by S. intermedius, however, varies with physiological temperature changes. These results may assist in understanding possible behavior relative to health and disease.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"492-7"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00460.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27821069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Sodium fluoride and chlorhexidine effect in the inhibition of mutans streptococci in children with dental caries: a randomized, double-blind clinical trial. 氟化钠和氯己定对龋齿儿童变形链球菌的抑制作用:一项随机、双盲临床试验

Oral microbiology and immunology Pub Date : 2008-12-01 DOI: 10.1111/j.1399-302X.2008.00458.x

P L D Lobo, C B M de Carvalho, S G C Fonseca, R S L de Castro, A J Monteiro, M C Fonteles, C S R Fonteles

{"title":"Sodium fluoride and chlorhexidine effect in the inhibition of mutans streptococci in children with dental caries: a randomized, double-blind clinical trial.","authors":"P L D Lobo, C B M de Carvalho, S G C Fonseca, R S L de Castro, A J Monteiro, M C Fonteles, C S R Fonteles","doi":"10.1111/j.1399-302X.2008.00458.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00458.x","url":null,"abstract":"Objective: We aimed to compare the effect of sodium fluoride and chlorhexidine on salivary levels of mutans streptococci (MS), in a double-blind, randomized clinical trial.Methods: Thirty-five healthy volunteers, aged 4-8 years, with at least one active carious lesion and no previous history of allergies were selected to participate in the study. A gel formulation containing either 1.23% sodium fluoride or 1% chlorhexidine was topically administered to the dentition every 24 h for 6 consecutive days. Salivary MS levels were measured at baseline (D1) and on the 6th (D6), 15th (D15), and 30th (D30) days. For microbiological analysis, Mitis Salivarius-Bacitracin agar medium was used.Results: Difference between treatments was only verified on D6. On the last day of treatment 1% chlorhexidine gel was significantly more effective than fluoride (P = 0.0000). The use of sodium fluoride did not cause a statistically significant variation in salivary MS levels throughout the duration of the study. Following treatment, a subsequent increase in MS counts between D6 and D15 (P = 0.0001) was observed with chlorhexidine.Conclusion: A 6-day treatment with a 1% chlorhexidine gel was effective in reducing salivary MS; there was a significant MS increase once treatment was suspended. The use of 1.23% sodium fluoride under the same regimen was not able to reduce salivary MS levels. Our results suggest repeated treatment with 1% chlorhexidine as a means for maintaining low salivary MS levels in children with dental caries.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"486-91"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00458.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38