Differential effects of five Aggregatibacter actinomycetemcomitans strains on gingival epithelial cells.

Oral microbiology and immunology Pub Date : 2008-12-01 DOI:10.1111/j.1399-302X.2008.00449.x

T Shimada, N Sugano, R Nishihara, K Suzuki, H Tanaka, K Ito

{"title":"Differential effects of five Aggregatibacter actinomycetemcomitans strains on gingival epithelial cells.","authors":"T Shimada, N Sugano, R Nishihara, K Suzuki, H Tanaka, K Ito","doi":"10.1111/j.1399-302X.2008.00449.x","DOIUrl":null,"url":null,"abstract":"Introduction: We investigated gingival epithelial cell proliferation and expression of interleukin-8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) in response to Aggregatibacter actinomycetemcomitans serotypes a, b, and c.Methods: Human gingival cells (Ca9-22) were cultured in bacterial extracts prepared from five strains of A. actinomycetemcomitans: ATCC 43717 (serotype a); ATCC 29524, ATCC 29522, and ATCC 43718 (all serotype b); and ATCC 43719 (serotype c).Results: In bacterial extracts of ATCC 29522, cell growth was significantly impaired, while the expression of IL-8 and ICAM-1 was significantly increased. The level of induction in response to the other strains was minimal.Conclusion: Our results indicate that the five strains of A. actinomycetemcomitans have distinct effects on the abilities of human gingival epithelial cells to proliferate and to produce proinflammatory factors.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"455-8"},"PeriodicalIF":0.0000,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00449.x","citationCount":"26","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral microbiology and immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1399-302X.2008.00449.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 26

Abstract

Introduction: We investigated gingival epithelial cell proliferation and expression of interleukin-8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) in response to Aggregatibacter actinomycetemcomitans serotypes a, b, and c.

Methods: Human gingival cells (Ca9-22) were cultured in bacterial extracts prepared from five strains of A. actinomycetemcomitans: ATCC 43717 (serotype a); ATCC 29524, ATCC 29522, and ATCC 43718 (all serotype b); and ATCC 43719 (serotype c).

Results: In bacterial extracts of ATCC 29522, cell growth was significantly impaired, while the expression of IL-8 and ICAM-1 was significantly increased. The level of induction in response to the other strains was minimal.

Conclusion: Our results indicate that the five strains of A. actinomycetemcomitans have distinct effects on the abilities of human gingival epithelial cells to proliferate and to produce proinflammatory factors.

查看原文本刊更多论文

5株放线菌聚集菌对牙龈上皮细胞的差异影响。

研究了放线菌聚集菌a、b、c血清型对牙龈上皮细胞增殖及白细胞介素-8 (IL-8)和细胞间粘附分子1 (ICAM-1)表达的影响。方法:用5株放线菌提取液培养人牙龈细胞Ca9-22: ATCC 43717(血清型a);ATCC 29524、ATCC 29522和ATCC 43718(均为b型血清);结果:ATCC 29522菌提液细胞生长明显受损，IL-8和ICAM-1表达明显升高。对其他菌株的诱导反应水平是最低的。结论:5株放线菌对人牙龈上皮细胞的增殖能力和促炎因子的产生有明显的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Oral microbiology and immunology

自引率

0.00%

发文量