Oral microbiology and immunology最新文献

Scavenger receptor A is expressed by macrophages in response to Porphyromonas gingivalis, and participates in TNF-alpha expression. 清道夫受体A在对牙龈卟啉单胞菌的反应中由巨噬细胞表达，并参与tnf - α的表达。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00538.x

M T Baer, N Huang, F C Gibson

{"title":"Scavenger receptor A is expressed by macrophages in response to Porphyromonas gingivalis, and participates in TNF-alpha expression.","authors":"M T Baer, N Huang, F C Gibson","doi":"10.1111/j.1399-302X.2009.00538.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00538.x","url":null,"abstract":"Introduction: Porphyromonas gingivalis is a periodontopathic bacterium closely associated with generalized aggressive periodontal disease. Pattern recognition receptors (PRRs) participate in host response to this organism. It is likely that PRRs not previously recognized as part of the host response to P. gingivalis also participate in host response to this organism.Methods and results: Employing qRT-PCR, we observed increased msr1 gene expression at 2, 6, and 24 h of culture with P. gingivalis strain 381. Flow cytometry revealed increased surface expression of SR-A protein by the 24 h time point. Macrophages cultured with an attachment impaired P. gingivalis fimA- mutant (DPG3) expressed intermediate levels of SR-A expression. Heat-killed P. gingivalis stimulated SR-A expression similar to live bacteria, and purified P. gingivalis capsular polysaccharide stimulated macrophage SR-A expression, indicating that live whole organisms are not necessary for SR-A protein expression in macrophage response. As SR-A is known to play a role in lipid uptake by macrophages, we tested the ability of low-density lipoprotein (LDL) to influence the SR-A response of macrophages to P. gingivalis, and observed no effect of LDL on P. gingivalis-elicited SR-A expression. Lastly, we observed that SR-A knockout (SR-A(-/-)) mouse macrophages produced significantly more tumor necrosis factor (TNF)-alpha than wild type mouse macrophages cultured with P. gingivalis.Conclusion: These data identify that SR-A is expressed by macrophages in response to P. gingivalis, and support that this molecule plays a role in TNF-alpha production by macrophages to this organism.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"456-63"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00538.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Progression of chronic periodontitis can be predicted by the levels of Porphyromonas gingivalis and Treponema denticola in subgingival plaque. 慢性牙周炎的进展可以通过牙龈下菌斑中牙龈卟啉单胞菌和牙密螺旋体的水平来预测。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00544.x

S J Byrne, S G Dashper, I B Darby, G G Adams, B Hoffmann, E C Reynolds

{"title":"Progression of chronic periodontitis can be predicted by the levels of Porphyromonas gingivalis and Treponema denticola in subgingival plaque.","authors":"S J Byrne, S G Dashper, I B Darby, G G Adams, B Hoffmann, E C Reynolds","doi":"10.1111/j.1399-302X.2009.00544.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00544.x","url":null,"abstract":"Introduction: Chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. Diagnosis is achieved retrospectively by clinical observation of attachment loss. Predicting disease progression would allow for targeted preventive therapy. The aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of disease and levels of specific bacteria in the plaque samples to predict disease progression.Methods: During a 12-month longitudinal study of 41 subjects, 25 sites in 21 subjects experienced disease progression indicated by at least 2 mm of clinical attachment loss. Real-time polymerase chain reaction was used to determine the levels of Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum, and Prevotella intermedia in subgingival plaque samples.Results: No clinical parameters were able to predict periodontal disease progression. In sites undergoing imminent periodontal disease progression within the next 3 months, significant partial correlations were found between P. gingivalis and T. forsythia (r = 0.55, P < 0.001) and T. denticola and T. forsythia (r = 0.43, P = 0.04). The odds of a site undergoing imminent periodontal disease progression increased with increasing levels of P. gingivalis and T. denticola.Conclusion: Monitoring the proportions of P. gingivalis and T. denticola in subgingival plaque has the potential to help identify sites at significant risk for progression of periodontitis, which would assist in the targeted treatment of disease.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"469-77"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00544.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 183

Characterization of bacterial flora in persistent apical periodontitis lesions. 持续性根尖牙周炎病变细菌菌群特征分析。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00534.x

R Fujii, Y Saito, Y Tokura, K-I Nakagawa, K Okuda, K Ishihara

{"title":"Characterization of bacterial flora in persistent apical periodontitis lesions.","authors":"R Fujii, Y Saito, Y Tokura, K-I Nakagawa, K Okuda, K Ishihara","doi":"10.1111/j.1399-302X.2009.00534.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00534.x","url":null,"abstract":"Introduction: Microorganisms are able to survive and induce persistent infection in periapical tissues. The aim of this study was to investigate the composition of the microflora of persistent apical periodontitis lesions.Methods: Twenty apical lesion samples were obtained from 20 patients with chronic apical periodontitis by root end surgery and processed using aerobic or anaerobic culture techniques. All isolated strains were identified by 16S ribosomal DNA sequence analysis.Results: Seventy-four strains were isolated, belonging to 31 bacterial species obtained from the 20 apical lesions that were isolated. The majority of the strains were facultative anaerobes (51.6%). Propionibacterium acnes, Staphylococcus epidermidis, Pseudomonas aeruginosa and Fusobacterium nucleatum were isolated from 16.2, 9.5, 6.8 and 5.4% of the samples, respectively. Fifteen samples harboured more than one species. The predominant association was P. acnes, S. epidermidis and F. nucleatum.Conclusion: The microbiota of persistent apical periodontitis lesions is composed by diverse types of microorganisms with biofilm-forming capacity, including P. acnes, S. epidermidis and F. nucleatum.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"502-5"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00534.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28438457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

Genetic diversity and toxic activity of Aggregatibacter actinomycetemcomitans isolates. 放线菌聚集菌分离株的遗传多样性和毒性活性。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00547.x

D Kawamoto, E S Ando, P L Longo, A C R Nunes, M Wikström, M P A Mayer

{"title":"Genetic diversity and toxic activity of Aggregatibacter actinomycetemcomitans isolates.","authors":"D Kawamoto, E S Ando, P L Longo, A C R Nunes, M Wikström, M P A Mayer","doi":"10.1111/j.1399-302X.2009.00547.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00547.x","url":null,"abstract":"Introduction: Very little is known of the diversity and expression of virulence factors of serotypes of Aggregatibacter actinomycetemcomitans. Toxic activity on Chinese hamster ovary (CHO) cells and cdt and ltx genotyping were evaluated in A. actinomycetemcomitans serotypes.Methods: Forty-one A. actinomycetemcomitans isolates were analysed for CHO cell growth inhibition. Genotyping was performed by polymerase chain reactions specific to the ltx promoter region, serotype-specific and cdt region and by sequencing of cdtB.Results: cdtABC was detected in 40 strains. Analysis of the cdtA upstream region revealed 10 cdt genotypes. Toxicity to CHO cells was detected for 92.7% of the isolates; however, no correlation between the toxic activity and the cdt genotype was detected. Serotype c was more prevalent among Brazilian samples (68.0%). Four serotype b isolates from subjects with aggressive periodontitis were associated with high leukotoxin production and exhibited moderate to strong toxic activity in CHO cells, but were classified in different cdt genotypes. High levels of toxicity in CHO cells were not associated with a particular serotype; 57.1% of serotype a isolates presented low toxicity to CHO cells whereas the highly toxic strains belonged to serotypes b and c. Sequencing of cdtB revealed a single nucleotide polymorphism of amino acid 281 but this was not related to the toxic activity in CHO cells.Conclusion: Differences in prevalence of the low and highly cytotoxic strains among serotypes reinforce the hypothesis that serotype b and c isolates of A. actinomycetemcomitans are more virulent than serotype a strains.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"493-501"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00547.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28438456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis. 牙龈卟啉单胞菌I型和II型菌毛基因型的不同毒力和先天免疫相互作用。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00545.x

M Wang, S Liang, K B Hosur, H Domon, F Yoshimura, A Amano, G Hajishengallis

{"title":"Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis.","authors":"M Wang, S Liang, K B Hosur, H Domon, F Yoshimura, A Amano, G Hajishengallis","doi":"10.1111/j.1399-302X.2009.00545.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00545.x","url":null,"abstract":"Introduction: The fimA-encoded fimbriae of the periodontal pathogen Porphyromonas gingivalis display genetic diversity. Type I fimbriated P. gingivalis (Pg-I) has been most widely studied at the molecular level, whereas Pg-II is the most frequent isolate from severe periodontitis.Methods: To investigate virulence differences between Types I and II fimbriae, we examined strains 33277 (Pg-I) and OMZ314 (Pg-II), reciprocal swap mutants (i.e. expressing the heterologous fimbrial type), and their respective FimA-deficient derivatives. These organisms were tested in a mouse periodontitis model and in interactions with mouse macrophages, a cell type that plays important roles in chronic infections.Results: Strain 33277 induced significantly more periodontal bone loss than OMZ314 and substitution of Type II fimbriae with Type I in OMZ314 resulted in a more virulent strain than the parent organism. However, the presence of Type II fimbriae was associated with increased proinflammatory and invasive activities in macrophages.Conclusion: The inverse relationship between proinflammatory potential and ability to cause experimental periodontitis may suggest that an aggressive phenotype could provoke a host response that would compromise the persistence of the pathogen.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"478-84"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00545.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28438454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Distribution and hydrolytic enzyme characteristics of Candida albicans strains isolated from diabetic patients and their non-diabetic consorts. 糖尿病及非糖尿病患者白色念珠菌的分布及水解酶特性。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00524.x

M F G Boriollo, R C Bassi, C M G dos Santos Nascimento, L M Feliciano, S B Francisco, L M Barros, L C Spolidório, D M Palomari Spolidório

{"title":"Distribution and hydrolytic enzyme characteristics of Candida albicans strains isolated from diabetic patients and their non-diabetic consorts.","authors":"M F G Boriollo, R C Bassi, C M G dos Santos Nascimento, L M Feliciano, S B Francisco, L M Barros, L C Spolidório, D M Palomari Spolidório","doi":"10.1111/j.1399-302X.2009.00524.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00524.x","url":null,"abstract":"Introduction: The aim of this study was to investigate the oral colonization profile of Candida albicans strains isolated from diabetic patients and their non-diabetic consorts. In addition hydrolytic enzyme activity of these isolates was analysed.Methods: The genetic diversity of C. albicans oral isolates from 52 couples was established using isoenzyme marker and cluster analysis. Hydrolytic enzyme characteristics, namely secreted aspartyl proteinases (SAPs) and phospholipases (PLs) were also analysed.Results: Simultaneous colonization by C. albicans was observed in the consorts of 12 couples (23.1%). Patterns of monoclonal and polyclonal oral colonization by C. albicans strains were identified and the coexistence of identical or highly related strains was observed in both members of eight couples. The genetic diversity observed in the total yeast population revealed four large, genetically distinct groups (A to D) and the coexistence of strains in couples or consorts conjugally unrelated. SAP and PL activity was observed in the majority of C. albicans isolates without any association to particular strain, strain clusters (highly related isolates), or clinical characteristics of the consorts (diabetic, non-diabetic, and gender).Conclusion: Possible sources of transmission and oral propagation of groups (clusters) of strains of C. albicans can occur between diabetic and non-diabetic consorts. A conjugal genotypic identity exists in most C. albicans-positive couples, that is, both consorts share identical or highly related strains; however, this identity is not couple-specific as seen by the coexistence of clusters in couples and unrelated consorts.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"437-50"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00524.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Automutanolysin disrupts clinical isolates of cariogenic streptococci in biofilms and planktonic cells. 自解酶破坏临床分离的龋齿链球菌在生物膜和浮游细胞。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00536.x

P Thanyasrisung, H Komatsuzawa, G Yoshimura, T Fujiwara, S Yamada, K Kozai, K Eto, Y Izumi, M Sugai

{"title":"Automutanolysin disrupts clinical isolates of cariogenic streptococci in biofilms and planktonic cells.","authors":"P Thanyasrisung, H Komatsuzawa, G Yoshimura, T Fujiwara, S Yamada, K Kozai, K Eto, Y Izumi, M Sugai","doi":"10.1111/j.1399-302X.2009.00536.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00536.x","url":null,"abstract":"Introduction: Dental caries remains one of the most common chronic infectious diseases throughout the world. The formation of dental plaque is one of the caries risk factors. As a consequence, the removal of plaque may reduce the incidence of caries development. We identified an autolysin produced by Streptococcus mutans named auto-mutanolysin (Aml). Aml selectively lyses S. mutans and Streptococcus sobrinus. The specificity towards these cariogenic bacteria suggests that Aml may be used to prevent dental caries. Here, with the aim towards therapeutic application, we investigated the lytic activity of Aml against clinical isolates of S. mutans and S. sobrinus using planktonic cells and biofilms.Methods: Planktonic cell suspensions and biofilms of clinically isolated streptococci were treated with Aml in the absence or the presence of Triton X-100. The lytic activity of Aml was monitored as the change in turbidity. The disruption of biofilms was evaluated by detecting the released DNA by polymerase chain reaction and observing the alteration of optical density of treated biofilms.Results: Triton X-100 enhances the lytic ability of Aml. Using planktonic cells, Aml had various lysis levels against clinical strains. Repeated Aml treatment showed disruption of the biofilm using the representative clinical strains.Conclusion: Our study demonstrates that Aml has an ability to lyse planktonic and biofilm cells of clinically isolated mutans streptococci in the presence of Triton X-100. These results suggest the possibility of using Aml as an alternative or additional approach for caries prevention.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"451-5"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00536.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Characterization of L-cysteine desulfhydrase from Prevotella intermedia. 中间普雷沃氏菌l -半胱氨酸脱硫酶的研究。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00546.x

T Yano, H Fukamachi, M Yamamoto, T Igarashi

{"title":"Characterization of L-cysteine desulfhydrase from Prevotella intermedia.","authors":"T Yano, H Fukamachi, M Yamamoto, T Igarashi","doi":"10.1111/j.1399-302X.2009.00546.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00546.x","url":null,"abstract":"Introduction: Hydrogen sulfide is responsible for lysis of red blood cells and is a major compound for oral malodor. To clarify the production mechanism of hydrogen sulfide in Prevotella intermedia, we found an L-cysteine desulfhydrase gene (lcs) homologue on the genome database of P. intermedia ATCC25611 and characterized its gene product.Methods: The lcs gene homologue cloned into pGEX6p-1 vector was expressed in Escherichia coli and purified. Lcs activity was assayed by detection of the reaction products (hydrogen sulfide and pyruvate) or its derivatives from L-cysteine. Site-directed mutagenesis was used to convert an amino acid of the Lcs molecule.Results: The purified lcs gene product catalysed the degradation of L-cysteine to pyruvate, ammonia, and hydrogen sulfide, indicating that the protein is L-cysteine desulfhydrase. The enzyme required pyridoxal 5'-phosphate as a cofactor, and it was highly active at pH 7.0 and completely inhibited by ZnCl(2). The K(m) and V(max) of the enzyme were 0.7 mm and 4.2 micromol/min/mg, respectively. Replacement of Tyr-59, Tyr-118, Asp-198, and Lys-233 with any of the amino acids resulted in the complete disappearance of Lcs activity, implying that these amino acids are essential for enzyme activity. In addition, hydrogen sulfide produced by this enzyme lysed sheep red blood cells and modified hemoglobin.Conclusion: These results show the enzymatic properties of L-cysteine desulfhydrase from P. intermedia ATCC25611 and also suggest that the Lcs enzyme, which produces hydrogen sulfide from L-cysteine, is closely associated with the pathogenesis of P. intermedia.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"485-92"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00546.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28438455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment. 牙周病患者龈沟液中疱疹病毒的检测:患病率及治疗效果。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00542.x

G Grenier, G Gagnon, D Grenier

{"title":"Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment.","authors":"G Grenier, G Gagnon, D Grenier","doi":"10.1111/j.1399-302X.2009.00542.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00542.x","url":null,"abstract":"Background/aim: Although the role of bacteria in the etiology of periodontitis is well established, it has been suggested that herpetic viruses could contribute to the initiation and progression of this disease. The aim of this study was to determine the prevalence of human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and herpes simplex virus (HSV) in gingival crevicular fluid (GCF) samples obtained from periodontally healthy, gingivitis and periodontitis patients. In addition, the effect of periodontal treatment (scaling and root planing) on the persistence of herpetic viruses was evaluated in a sub-group of patients suffering from chronic periodontitis.Methods: The presence of viruses in GCF samples was assessed by a nested PCR amplification technique. The persistence of viruses in periodontal sites was evaluated following a scaling and root planing therapy.Results: A statistically significant higher prevalence of HCMV was observed in periodontitis patients as compared to healthy control subjects (35 vs. 8%, respectively; P = 0.0377). A trend for a higher prevalence of HSV was also noted in the periodontitis group, in comparison with healthy control subjects. In addition, a higher prevalence of HCMV was associated with deep periodontal pockets in subjects suffering from periodontitis. In the sub-group of periodontitis patients, periodontal therapy resulted in the elimination (HCMV and EBV) or reduction (HSV) of the herpetic viruses.Conclusions: This study showed that the prevalence of HCMV and HSV viruses in GCF is higher in patients suffering from periodontitis compared to periodontally healthy subjects, and that the prevalence of HCMV is higher in deep periodontal pockets. It also brought evidences that periodontal therapy may be associated with virus elimination in diseased sites.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"506-9"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00542.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28438458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

Influence of mouse prolactin-inducible protein in saliva on the aggregation of oral bacteria. 小鼠唾液催乳素诱导蛋白对口腔细菌聚集的影响。

Oral microbiology and immunology Pub Date : 2009-12-01 DOI: 10.1111/j.1399-302X.2009.00543.x

A Nistor, G Bowden, A Blanchard, Y Myal

{"title":"Influence of mouse prolactin-inducible protein in saliva on the aggregation of oral bacteria.","authors":"A Nistor, G Bowden, A Blanchard, Y Myal","doi":"10.1111/j.1399-302X.2009.00543.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00543.x","url":null,"abstract":"Introduction: Mouse prolactin-inducible protein (mPIP) is secreted in mouse saliva and has been found to bind oral bacteria, showing the highest affinity for streptococci. Comparisons between the oral flora of mPIP knockout mice and their wild-type controls showed differences in the genera colonizing the two groups of mice. These findings suggested a role for mPIP in the colonization of the mouse oral cavity, possibly modulating the oral flora. In this in vitro study, we focused on the contribution of this protein to aggregation of oral bacteria, a process thought to promote the clearance of bacteria from the oral cavity, and one that could influence the composition of the oral bacterial community.Methods: The aggregation of selected human and mouse oral streptococci was measured spectrophotometrically. The aggregation of oral bacteria by saliva from mPIP knockout mice, which lack mPIP, was compared with that of saliva from wild-type mice.Results: Both wild-type saliva and mPIP knockout mouse saliva induced aggregation of human strain Streptococcus gordonii SK120 and mouse streptococci strains M105/6 and M106/2. Bacterial aggregation induced by the saliva of wild-type mice was significantly higher than the aggregation induced by saliva from mPIP knockout mice for all the bacterial strains.Conclusion: In this study it was confirmed that mPIP plays a role in the aggregation of oral bacteria. The salivary components promoting aggregation of oral bacteria are considered to be part of the oral defense mechanisms so these findings provide insight into a possible function of mPIP in host defense by promoting aggregation of oral bacteria.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"510-3"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00543.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28438459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20