Oral microbiology and immunology最新文献

{"title":"Helicobacter pylori in the oral cavity is associated with gastroesophageal disease.","authors":"R Morales-Espinosa,&nbsp;A Fernandez-Presas,&nbsp;G Gonzalez-Valencia,&nbsp;S Flores-Hernandez,&nbsp;G Delgado-Sapien,&nbsp;J L Mendez-Sanchez,&nbsp;E Sanchez-Quezada,&nbsp;L Muñoz-Pérez,&nbsp;R Leon-Aguilar,&nbsp;J Hernandez-Guerrero,&nbsp;A Cravioto","doi":"10.1111/j.1399-302X.2009.00541.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00541.x","url":null,"abstract":"<p><strong>Background: </strong>In Mexico, more than 80% of the population is infected with Helicobacter pylori. The frequency of H. pylori detection in the oral cavity is unknown, as its relationship with gastroesophageal pathology.</p><p><strong>Aim: </strong>To detect the presence of H. pylori in the oral cavity in Mexican population by PCR and to determine its association with gastroesophageal disease.</p><p><strong>Methods: </strong>Patients were divided into two groups with different clinic conditions from whom gastric biopsy, dental plaque, and saliva samples were taken and analyzed. The first group comprised of hospitalized patients, the majority of whom were diagnosed with gastroesophageal disease, while the second group was selected from a dental clinic (ambulatory population) the majority of whom appeared to be healthy subjects.</p><p><strong>Results: </strong>H. pylori was detected in gastric biopsy, dental plaque and saliva samples by PCR using a set of specific primers for the signal sequence of the vacuolating cytotoxin gene; detection of H. pylori in general was higher in gastric biopsy and dental plaque samples than in saliva samples. Detection of H. pylori in the oral cavity is significantly (P = 0.0001) associated with patients presenting gastroesophageal disease, while healthy subjects and those with other non-gastric disease do not present with H. pylori in their oral cavity.</p><p><strong>Conclusions: </strong>H. pylori detection in the oral cavity is associated to gastroesophageal disease. In addition, it is suggested that all patients presenting gastric symptoms and H. pylori detection in the oral cavity would begin bacterial treatment immediately.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 6","pages":"464-8"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00541.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytomegalovirus-infected inflammatory cells in dental periapical lesions.","authors":"M Sabeti,&nbsp;A Daneshmand,&nbsp;J H Simon,&nbsp;J Slots","doi":"10.1111/j.1399-302X.2009.00540.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00540.x","url":null,"abstract":"<p><strong>Introduction: </strong>As cytomegalovirus may be etiologically involved in periapical pathosis of endodontic origin, this study aimed to determine the cellular source of periapical cytomegalovirus.</p><p><strong>Methods: </strong>Periapical granulomatous tissue was collected from 15 extracted teeth with symptomatic periapical lesions. Multi-color flow cytometry was used to identify cytomegalovirus-infected cells.</p><p><strong>Results: </strong>Cytomegalovirus infection was identified in 10 of the 15 (67%) study lesions, and in periapical monocytes/macrophages (40% of lesions) and T lymphocytes (54% of lesions), but not in periapical B lymphocytes.</p><p><strong>Conclusion: </strong>This study and previous polymerase chain reaction-based investigations show that cytomegalovirus is a frequent inhabitant of symptomatic periapical lesions.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"434-6"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00540.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28360656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Streptococcus mutans biofilm proteins recognized by salivary immunoglobulin A.","authors":"T Sanui,&nbsp;R L Gregory","doi":"10.1111/j.1399-302X.2009.00523.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00523.x","url":null,"abstract":"<p><strong>Introduction: </strong>The purpose of this study was to examine the Streptococcus mutans biofilm cellular proteins recognized by immunoglobulin A (IgA) in saliva from various caries-defined populations.</p><p><strong>Methods: </strong>Biofilm and planktonic S. mutans UA159 cells were prepared. The proteins were extracted, separated by two-dimensional gel electrophoresis, transferred to blotting membranes, and probed for IgA using individual saliva samples from three groups of subjects; those who developed 0 caries (no active caries), 5-9 caries (medium), or more than 10 caries (severe) over a 12-month interval.</p><p><strong>Results: </strong>Several proteins were recognized by salivary IgA in all groups of saliva but spot distribution and intensity varied greatly between the groups, and some proteins were recognized more strongly in biofilm cells than in planktonic culture, and vice versa. Furthermore, 15 proteins were only recognized by saliva from the 'no active caries' group, and four proteins were recognized by saliva samples from subjects in all three groups. Specifically, antigen I/II was recognized less in biofilm cells by caries-free saliva compared with planktonic cells. However, salivary IgA antibody to antigen I/II was absent in blots using saliva from the 'medium caries' and 'severe caries' groups.</p><p><strong>Conclusion: </strong>The bacterial molecules recognized by caries-free saliva are significant factors for S. mutans caries formation, and their inhibition could be a therapeutic target. In addition, saliva of caries-free subjects includes significant IgA antibody against antigen I/II of S. mutans, indicating a protective mechanism. However, microorganisms may protect themselves from host immune attack by forming biofilms and decreasing expression of antigen I/II.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"361-8"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00523.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28358181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory roles of beta-catenin and AP-1 on osteoprotegerin production in interleukin-1alpha-stimulated periodontal ligament cells.","authors":"T Suda,&nbsp;T Nagasawa,&nbsp;N Wara-Aswapati,&nbsp;H Kobayashi,&nbsp;K Iwasaki,&nbsp;R Yashiro,&nbsp;D Hormdee,&nbsp;H Nitta,&nbsp;I Ishikawa,&nbsp;Y Izumi","doi":"10.1111/j.1399-302X.2009.00529.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00529.x","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is a chronic inflammatory disease characterized by the enhanced expression of inflammatory mediators leading to alveolar bone resorption. Osteoprotegerin (OPG) plays a suppressive role in cytokine-induced osteoclastogenesis. In osteoblasts, OPG expression is upregulated by beta-catenin but downregulated by the transcription factor activator protein-1 (AP-1; c-fos/c-jun). The purpose of this study was to examine the roles of beta-catenin and AP-1 in interleukin-1alpha (IL-1alpha) -induced OPG production in human gingival fibroblasts (hGFs) and periodontal ligament (PDL) cells.</p><p><strong>Methods: </strong>Expression of c-fos and c-jun messenger RNA was measured by reverse transcription-polymerase chain reaction and OPG production was analysed by enzyme-linked immunosorbent assay. The nuclear AP-1 activity was quantified using an AP-1 microplate assay. The effect of the Wnt canonical pathway on OPG production was evaluated using small interfering (si) RNA for beta-catenin and the effect of AP-1 on OPG production was evaluated using the AP-1 inhibitor curcumin.</p><p><strong>Results: </strong>Levels of c-fos messenger RNA and nuclear AP-1 activity were higher in PDL cells than in hGFs. When stimulated with IL-1alpha, PDL cells had significantly higher c-fos expression and lower OPG production compared with hGFs. The siRNA for beta-catenin suppressed the IL-1alpha-induced OPG production in both PDL cells and hGFs, whereas the AP-1 inhibitor curcumin augmented the IL-1alpha-induced OPG production in PDL cells, but not in hGFs.</p><p><strong>Conclusion: </strong>The present study suggests that beta-catenin enhances IL-1alpha-induced OPG production in both PDL cells and hGFs, whereas AP-1 suppresses IL-1alpha-induced OPG production in PDL cells. Higher expression of c-fos in PDL cells than in hGFs may implicate a role of PDL cells in alveolar bone resorption in periodontitis.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"384-9"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00529.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28359743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T-RFLP-based mcrA gene analysis of methanogenic archaea in association with oral infections and evidence of a novel Methanobrevibacter phylotype.","authors":"M E Vianna,&nbsp;G Conrads,&nbsp;B P F A Gomes,&nbsp;H P Horz","doi":"10.1111/j.1399-302X.2009.00539.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00539.x","url":null,"abstract":"<p><strong>Introduction: </strong>Increasing evidence suggests a role for methanogenic archaea (methanogens) in human health and disease via syntrophic interactions with bacteria. Here we assessed the prevalence and distribution of methanogens and possible associations with bacteria in oral biofilms.</p><p><strong>Methods: </strong>Forty-four periodontal and 32 endodontic samples from necrotic teeth with radiographic evidence of apical periodontitis were analysed. Terminal restriction fragment length polymorphism analysis based on the mcrA gene, specific to methanogens, was applied. The prevalence and amounts of methanogens in endodontic samples were compared with those of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema spp. and Synergistes spp. based on real-time quantitative polymerase chain reactions.</p><p><strong>Results: </strong>Besides dominance of the mcrA gene corresponding to Methanobrevibacter oralis, one mcrA gene type, for which no cultivated member has been reported previously, was identified in five periodontal samples and one endodontic sample. Rates of non-synonymous vs. synonymous nucleotide substitutions suggest that this mcrA gene type codes for a functionally active methyl-coenzyme M reductase. Methanobrevibacter smithii, the prominent methanogen in the human gut system, was not detected. Mean proportions of methanogens were comparable to Synergistes spp. ranging from 0.5 to 1.0% of the total microbial community. Treponema spp. dominated with a mean proportion of 10%, while the mean proportions of the other endodontic pathogens were below 0.1%. A positive association between methanogens and Synergistes spp. was found.</p><p><strong>Conclusion: </strong>Our data provide evidence of a novel, as yet uncultured methanogenic phylotype in association with oral infections, and indicate possible interactions between methanogens and Synergistes spp., the nature of which deserves further investigation.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"417-22"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00539.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28359749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of serotype k Streptococcus mutans in Thai subjects.","authors":"J Lapirattanakul,&nbsp;K Nakano,&nbsp;R Nomura,&nbsp;H Nemoto,&nbsp;A Kojima,&nbsp;P Senawongse,&nbsp;R Srisatjaluk,&nbsp;T Ooshima","doi":"10.1111/j.1399-302X.2009.00530.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00530.x","url":null,"abstract":"<p><strong>Introduction: </strong>Streptococcus mutans, known to be a pathogen of dental caries as well as bacteremia and infective endocarditis, is classified into four serotypes, c, e, f and k, based on the structures of serotype-specific polysaccharides. Serotype k was recently designated using blood isolates from Japanese subjects and such strains are considered to be virulent in the bloodstream. The purpose of the present study was to analyse the serotype distribution of strains isolated from Thai subjects and determine whether serotype k strains were present.</p><p><strong>Methods: </strong>A total of 250 S. mutans strains were isolated from 50 Thai subjects, and serotypes of all strains were determined. Then, molecular and biological analyses were carried out for serotype k strains.</p><p><strong>Results: </strong>Immunodiffusion and polymerase chain reaction analyses showed that serotype c was the most prevalent (70%), followed by serotypes e (22.8%), f (4.4%) and k (2.8%), which indicated that serotype k S. mutans strains occurred in Thai individuals at a similar rate to that previously reported for Japanese and Finnish populations. Molecular analyses of the seven serotype k strains showed extremely low expression of rgpE, which is related to glucose side-chain formation in serotype-specific rhamnose-glucose polymers, similar to previous reports for those other populations. In addition, analysis of the biological properties of the seven serotype k strains demonstrated low levels of sucrose-dependent adhesion, cellular hydrophobicity, dextran-binding activity and phagocytosis susceptibility by human polymorphonuclear leukocytes, which are characteristics similar to those of serotype k strains previously isolated in Japan.</p><p><strong>Conclusion: </strong>Our results indicate the possibility of a worldwide prevalence of serotype k strains with properties in common with those of previously reported strains.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"431-3"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00530.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28360655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis mutant defective in a putative extracytoplasmic function sigma factor shows a mutator phenotype.","authors":"Y Kikuchi,&nbsp;N Ohara,&nbsp;O Ueda,&nbsp;K Hirai,&nbsp;Y Shibata,&nbsp;K Nakayama,&nbsp;S Fujimura","doi":"10.1111/j.1399-302X.2009.00526.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00526.x","url":null,"abstract":"<p><strong>Introduction: </strong>Porphyromonas gingivalis is implicated as a major pathogen in the development and progression of chronic periodontitis. P. gingivalis must possess the ability to tolerate stress signals outside the cytoplasmic membrane by transcriptional activation of genes encoding proteins involved in defense or repair processes. Some bacteria utilize a distinct subfamily of sigma factors to regulate extracytoplasmic function (hence termed the ECF subfamily).</p><p><strong>Methods: </strong>To elucidate their role in P. gingivalis, a chromosomal mutant carrying a disruption of an ECF sigma factor PG1318-encoding gene was constructed. Hemagglutination and proteolytic activities were measured in the PG1318-defective mutant. Reverse transcription-polymerase chain reaction (RT-PCR) analysis and southern blot analysis were used to assess transcription of kgp in the PG1318-defective mutant. Frequency of spontaneous mutation that conferred resistance to l-trifluoromethionine was measured in the PG1318-defective mutant.</p><p><strong>Results: </strong>The PG1318-defective mutant formed non-pigmented colonies on blood agar plates at a relatively high frequency. Arginine-specific and lysine-specific proteinase activities of the non-pigmented variants were remarkably decreased compared with those of the parent strain and the pigmented variants. RT-PCR analysis showed that kgp was not transcribed in some non-pigmented variants and southern blot analysis revealed that there was a deletion in their kgp region. Frequency of mutation conferring resistance to l-trifluoromethionine was significantly higher in the PG1318-defective mutant than in the wild-type.</p><p><strong>Conclusion: </strong>These results suggest that PG1318 plays a role in the regulation of mutation frequency in the bacterium.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"377-83"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00526.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28359742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interference with Aggregatibacter actinomycetemcomitans: colonization of epithelial cells under hydrodynamic conditions.","authors":"I Sliepen,&nbsp;M Van Essche,&nbsp;G Loozen,&nbsp;J Van Eldere,&nbsp;M Quirynen,&nbsp;W Teughels","doi":"10.1111/j.1399-302X.2009.00531.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00531.x","url":null,"abstract":"<p><strong>Introduction: </strong>Microbial interactions are considered to be important for bacterial colonization. Interactions that inhibit colonization of pathogens could possibly be used as a new treatment approach for periodontitis. The aim of this study was to test this hypothesis on soft surfaces in vitro, taking into account the hydrodynamic forces continuously present in vivo.</p><p><strong>Methods: </strong>Cultured epithelial cells were precolonized with Streptococcus sanguinis KTH-4, Streptococcus cristatus CC5A, Streptococcus salivarius TOVE and Streptococcus mitis BMS before Aggregatibacter actinomycetemcomitans colonization. Experiments were performed in a modified Robbins-device-type flow cell. Bacterial colonization and the number of epithelial cells were evaluated by microbial culturing and quantitative polymerase chain reaction.</p><p><strong>Results: </strong>The streptococci were able to inhibit A. actinomycetemcomitans colonization on soft tissue surfaces under flow conditions. Statistically significant differences were found between streptococcal pretreatments and the controls, with the most pronounced effect caused by S. sanguinis.</p><p><strong>Conclusion: </strong>These data confirm the possibility of applying beneficial bacteria in periodontal treatment.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"390-5"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00531.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28359744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis mediates the shedding and proteolysis of complement regulatory protein CD46 expressed by oral epithelial cells.","authors":"H Mahtout,&nbsp;F Chandad,&nbsp;J M Rojo,&nbsp;D Grenier","doi":"10.1111/j.1399-302X.2009.00532.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00532.x","url":null,"abstract":"<p><strong>Introduction: </strong>Human cells express membrane-bound complement regulatory proteins to prevent complement-mediated autologous tissue damage. In this study, we hypothesized that Porphyromonas gingivalis, the major etiological agent of chronic periodontitis, causes the shedding or proteolysis of the complement regulatory protein CD46 expressed by oral epithelial cells.</p><p><strong>Methods: </strong>Oral epithelial cells were treated with a culture of P. gingivalis before measurement of membrane-bound and shed CD46 by enzyme-linked immunosorbent assay (ELISA). The effect of soluble recombinant CD46 on secretion of interleukin-8 (IL-8) by epithelial cells was evaluated by ELISA. The susceptibility of soluble recombinant CD46 to proteolytic degradation by cells and purified Lys-gingipain of P. gingivalis was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis/western immunoblotting analysis.</p><p><strong>Results: </strong>Oral epithelial cells treated with a culture of P. gingivalis showed a lower reactivity with antibodies directed to CD46. ELISA revealed that such a treatment resulted in increased amounts of CD46 in the conditioned media suggesting that P. gingivalis caused the shedding of membrane-anchored CD46. Stimulation of epithelial cells with soluble recombinant CD46 induced IL-8 secretion in a dose-dependent manner. Whole cells and purified Lys-gingipain of P. gingivalis degraded recombinant CD46 in a dose-dependent manner.</p><p><strong>Conclusion: </strong>This study showed the ability of P. gingivalis to induce the shedding/ proteolysis of CD46 from the surface of oral epithelial cells. This may render host cells susceptible to the complement system and contribute to tissue damage and the inflammatory process in periodontitis.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"396-400"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00532.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28359745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"fimA genotypes and PFGE profile patterns in Porphyromonas gingivalis isolates from subjects with periodontitis.","authors":"P J Perez-Chaparro,&nbsp;A Rouillon,&nbsp;J Minet,&nbsp;G I Lafaurie,&nbsp;M Bonnaure-Mallet","doi":"10.1111/j.1399-302X.2009.00519.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00519.x","url":null,"abstract":"<p><strong>Background and objectives: </strong>Porphyromonas gingivalis is frequently identified to type by evaluation of fimA polymorphisms and less often by pulsed-field gel electrophoresis (PFGE) because of the technical intricacies of PFGE. To compare these techniques, we genotyped P. gingivalis clinical isolates as to (i) their fimA type and (ii) their whole genome restriction profile (PFGE analysis).</p><p><strong>Material and methods: </strong>Thirty-two P. gingivalis strains were isolated from 16 unrelated periodontitis patients. Two strains were isolated from each patient. Strains were subjected to a fimA-typing polymerase chain reaction (PCR) assay. Strains that could not be typed by PCR were submitted to sequencing of the entire fimA gene. The PFGE profiles of clinical strains were compared using bioinformatic analysis.</p><p><strong>Results: </strong>Seven of the 32 isolates were not typeable by PCR and so their entire fimA gene was sequenced. The sequencing identified each strain as belonging to a single fimA type. In one case, sequencing of the fimA gene did not agree with the result obtained using fimA PCR typing. With the exception of one patient, each patient presented isolates bearing the same fimA type. However, in three patients, isolates with the same fimA type presented different PFGE pulsotypes.</p><p><strong>Conclusion: </strong>The P. gingivalis typing using fimA PCR has limitations in typeability and discriminatory power. A typing technique for P. gingivalis that is easy to perform but that presents adequate typeability and discriminatory power is needed if we want to better understand the epidemiology of periodontal disease.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 5","pages":"423-6"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00519.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28360653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}