Oral microbiology and immunology最新文献

{"title":"Role of prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein-specific signal peptidase II (LspA) in localization and physiological function of lipoprotein MsmE in Streptococcus mutans.","authors":"T Arimoto,&nbsp;T Igarashi","doi":"10.1111/j.1399-302X.2008.00455.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00455.x","url":null,"abstract":"<p><strong>Introduction: </strong>To clarify the role that prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein-specific signal peptidase II (LspA) play in the physiological function of MsmE, we constructed lgt-deficient and lspA-deficient mutants of Streptococcus mutans 109c and examined the potential role of Lgt and LspA in membrane anchoring and growth in a melibiose medium of S. mutans.</p><p><strong>Methods: </strong>The lgt-, lspA-, and msmE-deficient mutants of S. mutans 109c were constructed by double-crossover recombination of their respective genes. Localization of MsmE was demonstrated by Western blot analysis with an MsmE antiserum. The growth of S. mutans cells was examined in a Trypton medium containing melibiose or glucose.</p><p><strong>Results: </strong>In the S. mutans lgt mutant, localization of the surface lipoprotein MsmE changed with the culture supernatant. The growth of the S. mutans lgt and lspA mutants was remarkably reduced in the melibiose medium; however, growth was recovered in the strains complemented with the lgt or the lspA gene. Therefore, lipid-modification by Lgt and subsequent signal peptide cleavage by LspA were crucial for membrane anchoring and the physiological function of MsmE in S. mutans.</p><p><strong>Conclusion: </strong>These results demonstrate that MsmE is required for melibiose metabolism in S. mutans and that modification by Lgt and LspA are important processes for the physiological function of MsmE.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"515-9"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00455.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27821073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genotypic identification of Candida dubliniensis from subgingival sites in immunocompetent subjects in Argentina.","authors":"V M Jewtuchowicz,&nbsp;M T Mujica,&nbsp;M I Brusca,&nbsp;N Sordelli,&nbsp;M C Malzone,&nbsp;S J Pola,&nbsp;C A Iovannitti,&nbsp;A C Rosa","doi":"10.1111/j.1399-302X.2008.00465.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00465.x","url":null,"abstract":"<p><strong>Introduction: </strong>It is generally recognized that Candida dubliniensis is commonly found in immunocompromised patients, such as those with advanced human immunodeficiency virus infection, at sites of periodontal disease. Since there are no data available for Argentina, the aim of this study was to determine the prevalence of and to identify C. dubliniensis in periodontal pockets from immunocompetent subjects living in Buenos Aires, Argentina, through a comparison of phenotypic and molecular assays.</p><p><strong>Methods: </strong>Yeasts recovered from subgingival plaque samples were studied for 180 immunocompetent non-smoking patients with periodontal disease. Yeasts were identified by conventional mycological methods and by specific polymerase chain reaction (PCR) assay. Fluconazole and voriconazole susceptibility studies were performed in keeping with the Clinical and Laboratory Standards Institute.</p><p><strong>Results: </strong>Among 76 yeasts isolated, C. dubliniensis comprised 10.5% (n = 8; 95% confidence interval 4.7-19.7), which corresponded to 4.4% of patients studied (8/180). C. albicans was the most frequently isolated species of yeast. A great majority of C. dubliniensis isolates was susceptible with only one isolate resistant to both antifungals.</p><p><strong>Conclusion: </strong>Micromorphology on Staib agar was the phenotypic method that was most concordant with PCR and it was useful for selecting presumptive C. dubliniensis. This is the first report to use PCR to identify C. dubliniensis in subgingival fluid from immunocompetent individuals with periodontal disease in Argentina. On the basis of the findings presented here, we confirm that C. dubliniensis can colonize periodontal pockets of immunocompetent patients with periodontal disease.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"505-9"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00465.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27821071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Participation of glutathione in the elimination of Porphyromonas gingivalis in vivo.","authors":"C Kato,&nbsp;M Mikami,&nbsp;T Natsuno","doi":"10.1111/j.1399-302X.2008.00436.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00436.x","url":null,"abstract":"<p><strong>Introduction: </strong>Glutathione is involved in immune responses such as cell proliferation and bactericidal activity. The aim of this study was to see whether glutathione influences the intraperitoneal elimination of Porphyromonas gingivalis in Fusobacterium nucleatum-immunized mice.</p><p><strong>Methods: </strong>Mice were immunized with P. gingivalis or F. nucleatum, and then P. gingivalis was inoculated into the peritoneal cavity of the mice. After various lengths of time, the numbers of bacteria were determined by a colony-forming assay and by polymerase chain reaction. The effect of glutathione on the elimination of P. gingivalis was explored by changing the intracellular glutathione level. Furthermore, we examined the effects of glutathione on the peritoneal levels of interferon-gamma, a macrophage activator, and of nitrite, a derivative of nitric oxide that acts as an antimicrobial agent when produced by macrophages and neutrophils.</p><p><strong>Results: </strong>Inoculated P. gingivalis was eliminated more rapidly from F. nucleatum-immunized mice than from P. gingivalis-immunized mice. Interferon-gamma levels in peritoneal lavage fluid and glutathione levels in peritoneal exudate cells were higher in F. nucleatum-immunized mice than in P. gingivalis-immunized mice. When P. gingivalis-immunized mice were given glutathione monoethylester (a derivative of glutathione that is converted to glutathione intracellularly through hydrolysis) into the peritoneal cavity, the elimination of P. gingivalis was accelerated. On the other hand, when F. nucleatum-immunized mice were given L-buthionine-[S,R]-sulfoximine (an inhibitor of glutathione synthesis) into the peritoneal cavity, the elimination of P. gingivalis was suppressed.</p><p><strong>Conclusion: </strong>In F. nucleatum-immunized mice, glutathione may have a key role in the defense against P. gingivalis infections.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"441-8"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00436.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible role of the adhesin ace and collagen adherence in conveying resistance to disinfectants on Enterococcus faecalis.","authors":"G Kayaoglu,&nbsp;H Erten,&nbsp;D Ørstavik","doi":"10.1111/j.1399-302X.2008.00446.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00446.x","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to evaluate whether the presence of the ace gene and Ace-mediated binding to collagen confers on Enterococcus faecalis resistance against common endodontic disinfectants.</p><p><strong>Methods: </strong>Isogenic strains of E. faecalis: OG1RF (wild-type) and TX5256 (ace insertion mutant of OG1RF) were grown in brain-heart infusion broth at 46 degrees C overnight. Standardized bacterial suspensions were pretreated for 1 h either with acid-soluble collagen or acidified phosphate-buffered saline (ac-PBS). Bacteria were challenged with chlorhexidine digluconate (CHX), iodine potassium-iodide (IKI), sodium hypochlorite (NaOCl), and calcium hydroxide [Ca(OH)(2)]. Samples were removed at 1, 3, and 6 h, and cultured on Todd-Hewitt agar plates. Colonies were counted, the absolute values were log transformed, and the data were statistically analyzed using Fisher's least significant differences test and t-test.</p><p><strong>Results: </strong>OG1RF was more resistant than TX5256 to IKI, NaOCl, and Ca(OH)(2) (P < 0.05). Collagen-exposed OG1RF was more resistant than the ac-PBS-pretreated OG1RF against CHX at 3 h and against IKI at 1 h (P < 0.05); no significant difference was found against NaOCl. As expected, the ace mutant strain, TX5256, pretreated with collagen or ac-PBS did not differ significantly in viability when challenged with CHX, IKI, and NaOCl. An unexpected result was found for Ca(OH)(2): collagen-pretreated OG1RF and TX5256 were both more susceptible than ac-PBS-pretreated OG1RF and TX5256, respectively (P < 0.05).</p><p><strong>Conclusion: </strong>The presence of the ace gene confers resistance against IKI, NaOCl, and Ca(OH)(2) on E. faecalis. Exposure to collagen makes the wild-type bacterium more resistant against CHX and IKI; however, exposure to collagen apparently decreases resistance to Ca(OH)(2).</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"449-54"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00446.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sugar fermentation in probiotic bacteria--an in vitro study.","authors":"M Hedberg,&nbsp;P Hasslöf,&nbsp;I Sjöström,&nbsp;S Twetman,&nbsp;C Stecksén-Blicks","doi":"10.1111/j.1399-302X.2008.00457.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00457.x","url":null,"abstract":"<p><strong>Introduction: </strong>Food supplemented with probiotic bacteria is a rapidly growing sector of the market. The aim of the present study was to evaluate and compare the acid production of selected probiotic strains available in commercial products.</p><p><strong>Methods: </strong>Six Lactobacillus strains (Lactobacillus plantarum 299v and 931; Lactobacillus rhamnosus GG and LB21; Lactobacillus paracasei subsp. paracasei F19, and Lactobacillus reuteri PTA 5289) were cultivated at 37 degrees C in an anaerobic atmosphere on Man, Rogosa, Shape (MRS) agar for 48 h or MRS broth for 16 h. After centrifugation, the cells were washed and resuspended in sterile phosphate-buffered saline and immediately subjected to a fermentation assay with 12 different carbohydrates (nine sugars and three sugar alcohols) in microtiter plates with a pH indicator. The plates were examined for color changes after 24, 48, and 72 h of incubation under aerobic and anaerobic conditions. Three scores were used: negative (pH > 6.8); weak (pH 5.2-6.8), and positive (pH < 5.2). The strains were characterized with the API 50 CH system to confirm their identity.</p><p><strong>Results: </strong>L. plantarum fermented all the sugars except for melibiose, raffinose, and xylitol. Both L. rhamnosus strains were generally less active although L. rhamnosus GG was slightly more active than strain LB21 in the 5% CO(2) setting. The latter strain exhibited negative reactions for sucrose, maltose, arabinose, and sorbitol under anaerobic conditions. The assays with L. paracasei and L. reuteri had negative or weak reactions for all tested sugars under both aerobic and anaerobic conditions.</p><p><strong>Conclusion: </strong>The metabolic capacity to form acid from dietary sugars differed significantly between the various probiotic strains.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"482-5"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00457.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27820626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggregatibacter actinomycetemcomitans adhesion inhibited in a flow cell.","authors":"I Sliepen,&nbsp;J Hofkens,&nbsp;M Van Essche,&nbsp;M Quirynen,&nbsp;W Teughels","doi":"10.1111/j.1399-302X.2008.00456.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00456.x","url":null,"abstract":"<p><strong>Introduction: </strong>Microbial interactions are considered important in the adhesion process of pathogenic bacteria in the oral cavity. This study addressed the hypothesis that a streptococcal biofilm influences the hard tissue colonization by the periodontopathogen Aggregatibacter actinomycetemcomitans under hydrodynamic conditions.</p><p><strong>Methods: </strong>The colonization of a green-fluorescent-protein-labelled A. actinomycetemcomitans strain on surfaces coated with a streptococcal biofilm, was monitored in real time using a confocal laser scanning microscope-mounted flow cell. Culture and quantitative polymerase chain reaction data were obtained in parallel from a Modified Robbins Device.</p><p><strong>Results: </strong>Colonization of A. actinomycetemcomitans was inhibited by the four tested streptococci (Streptococcus sanguinis, Streptococcus cristatus, Streptococcus salivarius, and Streptococcus mitis). The most inhibiting species was S. sanguinis.</p><p><strong>Conclusion: </strong>These results confirmed the hypothesis that some bacterial species influence A. actinomycetemcomitans colonization of hard surfaces in vitro under hydrodynamic conditions.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"520-4"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00456.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27821074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization of hard and soft surfaces by Aggregatibacter actinomycetemcomitans under hydrodynamic conditions.","authors":"I Sliepen,&nbsp;M Van Essche,&nbsp;M Pauwels,&nbsp;J Van Eldere,&nbsp;J Hofkens,&nbsp;M Quirynen,&nbsp;W Teughels","doi":"10.1111/j.1399-302X.2008.00461.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00461.x","url":null,"abstract":"<p><strong>Introduction: </strong>Oral bacteria must attach to hard and soft tissues to colonize the oral cavity in the presence of a variety of forces caused by shear and flow. In vitro models mimicking this dynamic process are indispensable to study factors that might interfere with the first step towards infection. For extrapolation purposes the comparability between the dynamics of colonization on hard vs. soft surfaces needs to be evaluated.</p><p><strong>Methods: </strong>The colonization of glass and epithelial cell surfaces by the periodontal pathogen Aggregatibacter actinomycetemcomitans was followed in time with two flow cell models: a modified Robbins device (MRD) and an in situ image analysis system.</p><p><strong>Results: </strong>The number of A. actinomycetemcomitans recovered from the soft surfaces in the MRD experiments was higher than on glass. The amount of bacteria on the hard surfaces kept increasing with time, while on soft surfaces saturation was reached. The microscope-mounted flow cell allowed real-time in situ monitoring of the colonization process of both surfaces.</p><p><strong>Conclusion: </strong>These experimental models may have a great contribution to make in the development of new treatment approaches for periodontal diseases. Colonization by A. actinomycetemcomitans could be studied under flow conditions and its dynamics showed important surface-dependent characteristics.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 6","pages":"498-504"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00461.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27821070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI-TOF-MS.","authors":"C S Stîngu,&nbsp;A C Rodloff,&nbsp;H Jentsch,&nbsp;R Schaumann,&nbsp;K Eschrich","doi":"10.1111/j.1399-302X.2008.00438.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00438.x","url":null,"abstract":"<p><strong>Introduction: </strong>To facilitate the identification of anaerobes cultivated from periodontal disease, whole cell bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was evaluated.</p><p><strong>Methods: </strong>A total of 84 strains (nine reference strains and 75 recent clinical isolates from 33 patients with aggressive periodontitis) previously identified with phenotypic methods were used. All the references and 10 clinical isolates belonging to the same species as the reference strains were genotypically identified by sequence analysis of the 16S ribosomal RNA gene. All the strains were then analyzed using MALDI-TOF-MS.</p><p><strong>Results: </strong>The reference strains of anaerobic bacteria used showed characteristic MALDI-TOF-MS spectra with peaks between m/z 2000 and up to about m/z 13,000. On visual inspection, the similarity of spectra produced by strains of a single genus could be recognized. Obvious differences between spectra produced by strains of different species were also easily noticed. The reproducibility of the method was proved by the similarity of spectra belonging to the same species. The spectra of the Prevotella intermedia strains identified with MALDI clustered together and clustered separately from the spectra of Prevotella nigrescens, proving that MALDI-TOF-MS is an accurate method that is capable of separating these two species. The quality of clustering was characterized by calculating an inconsistency coefficient (Mathworks:/Matlab Reference Manual v2007a/, Statistical toolbox).</p><p><strong>Conclusion: </strong>Our results suggest that MALDI-TOF-MS might become a useful method for the identification of anaerobic bacteria, especially for those that cannot be readily identified by biochemical analysis. It may become an attractive system even for the routine identification of clinical isolates.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 5","pages":"372-6"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00438.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27675532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens.","authors":"L G Marcato,&nbsp;A P Ferlini,&nbsp;R C F Bonfim,&nbsp;M L Ramos-Jorge,&nbsp;C Ropert,&nbsp;L F C Afonso,&nbsp;L Q Vieira,&nbsp;A P R Sobrinho","doi":"10.1111/j.1399-302X.2008.00432.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00432.x","url":null,"abstract":"<p><strong>Introduction: </strong>Periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. Recently identified Toll-like receptors (TLR) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. However, very little information is available on the role of TLR in the induction of periapical lesions.</p><p><strong>Method: </strong>The role of TLR-2 and TLR-4 in the activation of murine macrophages stimulated using Fusobacterium nucleatum and Peptostreptococcus anaerobius was investigated. The production of nitric oxide (NO) and reactive oxygen species (ROS) was assessed.</p><p><strong>Results: </strong>The results demonstrate that TLR-2 and TLR-4 are involved in the production of ROS by activated macrophages. The microorganisms induced similar levels of NO production by TLR-2-competent and TLR-2-deficient macrophages, regardless of the addition of interferon-gamma (IFN-gamma), ruling out a role for TLR-2 in the NO production induced by these bacteria. Only P. anaerobius induced NO production by TLR-4-competent macrophages without the addition of IFN-gamma. However, after IFN-gamma addition, F. nucleatum induced macrophage NO production. Therefore, NO production stimulated by IFN-gamma and these microorganisms seems to be TLR-4-independent.</p><p><strong>Conclusion: </strong>TLR-2 seems to be involved in the induction of ROS production by macrophages in response to prevalent root canal bacteria, while only F. nucleatum induced ROS production by TLR-4-competent macrophages. Both microorganisms significantly induced large amounts of NO independent of TLR-2 and TLR-4. We conclude that microorganisms may participate in the induction and progression of periapical lesions through NO and ROS production by activated macrophages.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 5","pages":"353-9"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00432.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27676682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mimicry of Aggregatibacter actinomycetemcomitans with beta2 glycoprotein I.","authors":"D Wang,&nbsp;T Nagasawa,&nbsp;Y Chen,&nbsp;Y Ushida,&nbsp;H Kobayashi,&nbsp;Y Takeuchi,&nbsp;M Umeda,&nbsp;Y Izumi","doi":"10.1111/j.1399-302X.2008.00442.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00442.x","url":null,"abstract":"<p><strong>Introduction: </strong>beta2-Glycoprotein I (beta 2GPI) is important in the suppression of coagulation, and antibodies against TLRVYK peptides on the beta 2GPI molecule are related to thrombosis. According to the Swiss-Prot database, Aggregatibacter actinomycetemcomitans leukotoxin c has sequences (SIRVYK) that are homologous to the TLRVYK peptides. The aim of this study was to investigate the effects of A. actinomycetemcomitans infection on the antibody response against SIRVYK peptides in patients with periodontitis.</p><p><strong>Methods: </strong>Serum immunoglobulin G (IgG) antibody and IgG subclass antibody titers against SIRVYK or TLRVYK peptides were measured by enzyme-linked immunosorbent assay in 46 patients with aggressive periodontitis (eight with localized disease, 38 with generalized disease), 28 patients with chronic periodontitis, and 20 periodontally healthy subjects. The presence of A. actinomycetemcomitans in plaque and saliva samples was determined using polymerase chain reaction.</p><p><strong>Results: </strong>The level of anti-SIRVYK antibodies was significantly higher in patients who were A. actinomycetemcomitans-positive than in A. actinomycetemcomitans-negative patients (P < 0.05) in the chronic periodontitis group. A similar trend was found in the antibody response to TLRVYK peptide; however, no statistically significant difference was seen between A. actinomycetemcomitans-positive and -negative patients. The A. actinomycetemcomitans-positive patients displayed significantly higher levels of anti-SIRVYK IgG2 and IgG3 antibodies than A. actinomycetemcomitans-negative patients (P < 0.05 and P < 0.05, respectively). The level of IgG2 was highest among the four IgG subclasses and it predominantly increased in patients who were A. actinomycetemcomitans-positive. Anti-TLRVYK antibody levels were significantly correlated with anti-SIRVYK IgG antibody levels.</p><p><strong>Conclusion: </strong>The results suggest that A. actinomycetemcomitans infection may elicit anti-SIRVYK IgG antibodies and modify the anti-TLRVYK antibody response in patients with periodontitis by molecular mimicry with beta2GPI.</p>","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"23 5","pages":"401-5"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00442.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27675536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}