Open Biology最新文献_第2页

The biology and pathophysiology of the proatherogenic metalloprotease ADAMTS7. 促动脉粥样硬化金属蛋白酶ADAMTS7的生物学和病理生理学研究。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-04-01 DOI: 10.1098/rsob.250111

Shiwali Goyal, James Muniu, Mohd Akbar Bhat, Eli Oldham, Derrick W Pruitt, Luis Frias Perez, Frida Z Miranda, Joshua Muia

{"title":"The biology and pathophysiology of the proatherogenic metalloprotease ADAMTS7.","authors":"Shiwali Goyal, James Muniu, Mohd Akbar Bhat, Eli Oldham, Derrick W Pruitt, Luis Frias Perez, Frida Z Miranda, Joshua Muia","doi":"10.1098/rsob.250111","DOIUrl":"https://doi.org/10.1098/rsob.250111","url":null,"abstract":"The ADAMTS (a disintegrin and metalloproteinase with thrombospondin type-1 motifs) metalloprotease family comprises 19 members, and they play critical roles in development, angiogenesis and coagulation. These enzymes are also pivotal in the turnover of extracellular matrix proteins in various tissues, and their dysregulation has been linked to diseases such as arthritis, atherosclerosis, cancer and inflammation. These secreted zinc metalloproteases are composed of multiple domains and are localized to the extracellular matrix and present in circulation. The metalloprotease member 7 of this family or ADAMTS7 was cloned in 2004. However, in 2011, it gained significant attention when its gene locus was associated with an increased risk of atherosclerosis and coronary artery disease. Despite its strong association with human diseases, the biological functions, substrate targets and processing of ADAMTS7 remain poorly understood, limiting our comprehension of its roles in both health and disease. This review highlights the structural organization of ADAMTS7, its expression and regulation, known substrates, and its involvement in various pathophysiological processes. Additionally, perspectives on future research directions and priorities are presented. ADAMTS7 remains a compelling target for drug and biomarker discovery, though much about this metalloprotease remains to be uncovered.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147593782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of stress sensitivity in memory formation: why do some animals learn while others do not? Lessons from Lymnaea stagnalis. 探索应激敏感性在记忆形成中的作用：为什么有些动物能学习而有些动物不能？从停滞林的经验教训。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-25 DOI: 10.1098/rsob.250283

Veronica Rivi, Kate Yakubets, Grace Pele, Anuradha Batabyal, Johanna M C Blom, Fabio Tascedda, Cristina Benatti, Ken Lukowiak

{"title":"Exploring the role of stress sensitivity in memory formation: why do some animals learn while others do not? Lessons from Lymnaea stagnalis.","authors":"Veronica Rivi, Kate Yakubets, Grace Pele, Anuradha Batabyal, Johanna M C Blom, Fabio Tascedda, Cristina Benatti, Ken Lukowiak","doi":"10.1098/rsob.250283","DOIUrl":"https://doi.org/10.1098/rsob.250283","url":null,"abstract":"The ability to learn and form memory is critical for survival, yet even genetically similar individuals can vary considerably in their cognitive performance. Using the pond snail Lymnaea stagnalis, we investigated how individual sensitivity to stress influences configural learning-a higher-order form of associative learning in which the simultaneous exposure to two contrasting stimuli, such as a predatory odour and an appetitive taste, results in the appetitive stimulus becoming associated with risk and evoking anti-predator behaviours. We used freshly collected, predator-naive snails from Margo Lake, Canada. While group-level data suggested the Margo strain failed to learn, individual-level analysis revealed that some snails successfully formed configural memories, while others did not. We hypothesized that this divergence reflects differences in individual (predator-related) stress responsiveness, which may modulate the engagement of memory-related molecular pathways. To test this, we measured expression levels of selected genes in the central ring ganglia. Snails that formed configural memories showed significantly higher expression of stress-responsive genes, components of the serotonin pathway and markers of neuroplasticity, along with increased endocannabinoid turnover. These findings suggest that individual variation in stress reactivity can drive adaptive differences in cognitive performance, offering new insights into the molecular and behavioural mechanisms underlying learning and memory.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STIM1 and endoplasmic reticulum-plasma membrane contact sites oscillate independently of calcium-induced calcium release. STIM1和内质网-质膜接触位点的振荡独立于钙诱导的钙释放。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-18 DOI: 10.1098/rsob.250220

Ding Xiong, Cheesan Tong, Suet Yin Sarah Fung, Samantha McClellan, Yang Yang, Jeffery Yong, Min Wu

{"title":"STIM1 and endoplasmic reticulum-plasma membrane contact sites oscillate independently of calcium-induced calcium release.","authors":"Ding Xiong, Cheesan Tong, Suet Yin Sarah Fung, Samantha McClellan, Yang Yang, Jeffery Yong, Min Wu","doi":"10.1098/rsob.250220","DOIUrl":"10.1098/rsob.250220","url":null,"abstract":"Calcium (Ca²+) release from intracellular stores, Ca²+ entry across the plasma membrane and their coordination via store-operated Ca²+ entry (SOCE) are critical for receptor-activated Ca²+ oscillations. However, the precise mechanism of Ca²+ oscillations and whether their control loop resides at the plasma membrane or intracellularly remains unresolved. By examining the dynamics of stromal interaction molecule 1 (STIM1), an endoplasmic reticulum (ER)-localized Ca²+ sensor that activates the Orai1 channel on the plasma membrane for SOCE, in mast cells, we found that a significant proportion of cells exhibited STIM1 oscillations with the same periodicity as Ca²+ oscillations. These cortical oscillations, shared with ER-plasma membrane (ER-PM) contact site proteins, were only detectable using total internal reflection fluorescence microscopy. Notably, STIM1 oscillations could occur independently of Ca²+ oscillations. Simultaneous imaging of cytoplasmic Ca²+ and ER Ca²+ with CEPIA1er revealed that receptor activation does not deplete ER Ca²+, whereas receptor activation without extracellular Ca²+ influx induces cyclic ER Ca²+ depletion. However, under such non-physiological conditions, cyclic ER Ca²+ oscillations lead to sustained STIM1 recruitment, indicating that oscillatory Ca²+ release is neither necessary nor sufficient for STIM1 oscillations. Using optogenetic tools to manipulate ER-PM contact site dynamics, we found that persistent ER-PM contact sites reduced the amplitude of Ca²+ oscillations without alteration of oscillation frequency. Together, these findings suggest an active cortical mechanism governs the rapid dissociation of ER-PM contact sites, thereby controlling amplitude of oscillatory Ca²+ dynamics during receptor-induced Ca²+ oscillations.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Know thyself. 认识你自己。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-11 DOI: 10.1098/rsob.260065

Jonathon Pines

引用次数: 0

Transcriptome-wide identification and expression profiling of chemosensory genes in rice grasshopper Oxya intricata (Orthoptera: Acrididae). 稻蝗化学感觉基因转录组鉴定及表达谱分析（直翅目：蝗科）。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-11 DOI: 10.1098/rsob.250373

Ran Li, Chao Xue, Dongkai Liu, Dan Zhang, Liyingzi He, Yujian Li, Xianfeng Yi

{"title":"Transcriptome-wide identification and expression profiling of chemosensory genes in rice grasshopper Oxya intricata (Orthoptera: Acrididae).","authors":"Ran Li, Chao Xue, Dongkai Liu, Dan Zhang, Liyingzi He, Yujian Li, Xianfeng Yi","doi":"10.1098/rsob.250373","DOIUrl":"https://doi.org/10.1098/rsob.250373","url":null,"abstract":"Chemosensory systems play an essential role in insect survival and reproduction, mediating behaviours such as host location, mating and oviposition. The rice grasshopper Oxya intricata is an important rice pest in Asia, yet the molecular basis of its olfaction remains poorly understood. In this study, we sequenced antennal transcriptomes from adult males and females and systematically identified chemosensory-related genes. A total of 104 candidates were identified, including 14 odorant-binding proteins (OBPs), 12 chemosensory proteins (CSPs), 67 odorant receptors (ORs), nine ionotropic receptors (IRs) and two sensory neuron membrane proteins (SNMPs). Expression profiling by quantitative real-time PCR revealed broad antennal enrichment across families, with subsets showing antenna-specific or sex-biased patterns, suggesting differential chemosensory deployment between tissues and sexes. Several OBPs, CSPs and ORs were also elevated in maxillary palps or tarsi, raising the possibility that these genes may be associated with contact or near-field chemosensation. Collectively, these results provide the first comprehensive survey of chemosensory genes in O. intricata and establish a molecular resource for future functional analyses of odour coding. The identified gene set offers tractable targets for ligand screening and behavioural assays, and may inform future efforts to develop semiochemical-based monitoring and management strategies for this economically important pest.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CDCA2-MYC positive feedback loop controls cancer cells survival. CDCA2-MYC正反馈回路控制着癌细胞的存活。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-11 DOI: 10.1098/rsob.250075

Konstantinos Stamatiou, Lorena Ligammari, Malki Bothota, Antonia Tsavou, Ezgi Gokhan, Ylenia Cicirò, Mariano Zacarias, Laura Soucek, Arturo Sala, Paola Vagnarelli

{"title":"A CDCA2-MYC positive feedback loop controls cancer cells survival.","authors":"Konstantinos Stamatiou, Lorena Ligammari, Malki Bothota, Antonia Tsavou, Ezgi Gokhan, Ylenia Cicirò, Mariano Zacarias, Laura Soucek, Arturo Sala, Paola Vagnarelli","doi":"10.1098/rsob.250075","DOIUrl":"https://doi.org/10.1098/rsob.250075","url":null,"abstract":"Cellular myelocytomatosis oncogene (MYC) transcription factors are encoded by a family of genes that include the prototype member MYC, MYCN and MYCL, and most human cancers display expression alterations of MYC genes. MYC is regulated at multiple levels, and its stability and activity are modulated by protein phosphorylation. Although there is a reasonable knowledge of the kinases required for MYC modifications, the counteracting phosphatases have been understudied. Here, we have investigated the role of the chromatin-associated protein phosphatase 1 (PP1) regulatory subunit CDCA2, also known as Repo-Man, in the regulation of MYC proteins in cancer cells. Using RNA interference and degron-mediated degradation of CDCA2, we have demonstrated that the PP1 subunit is required for cMYC and MYCN stabilization and viability of triple-negative breast cancer, neuroblastoma and colon cancer cells. Proximity ligation assays indicate that both cMYC and MYCN are in close proximity to CDCA2 in vivo. Furthermore, we have shown that CDC2A is a bona fide MYC target gene in cancer cells, revealing a reciprocal regulatory loop that could be exploited for therapeutic purposes.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FRET-based fluorescent sensors for cytosolic and mitochondrial Ca2+ imaging in Drosophila melanogaster. 基于fret的荧光传感器用于黑腹果蝇细胞质和线粒体Ca2+成像。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-11 DOI: 10.1098/rsob.250197

Manuela Santalla, Sonia Sonda, Ambra Bertocco, Alejandro Ciocci Pardo, Elisa Greotti, Diana Pendin

引用次数: 0

Using deep learning to predict the sex of human embryos. 利用深度学习来预测人类胚胎的性别。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-04 DOI: 10.1098/rsob.250419

Mingshu Liang, Magdalena Zernicka-Goetz, Adiyant Lamba, Dilan Gokyer, Catherine King, Maciej Meglicki, María Luisa Pardiñas, María José de Los Santos, Changhuei Yang

{"title":"Using deep learning to predict the sex of human embryos.","authors":"Mingshu Liang, Magdalena Zernicka-Goetz, Adiyant Lamba, Dilan Gokyer, Catherine King, Maciej Meglicki, María Luisa Pardiñas, María José de Los Santos, Changhuei Yang","doi":"10.1098/rsob.250419","DOIUrl":"10.1098/rsob.250419","url":null,"abstract":"The existence of sex differences in human pre-implantation development remains an open question, with previous attempts based on human observations yielding inconclusive results. In this study, we combined manual annotation and deep learning analysis of a dataset comprising 515 time-lapse embryo movies to investigate whether birth sex influences early developmental dynamics. While manual assessment did not identify any developmental timing parameters that reliably distinguish male from female embryos, a deep learning model trained and tested on these videos achieves a statistically significant sex prediction accuracy of 61%. Importantly, our analyses identified the period after the eight-cell stage as critical for accurate prediction, indicating that subtle sex-related differences may begin to emerge around day 3 of human embryogenesis. Studying sex differences at this early stage may enhance our understanding of why some embryos fail to develop and why sex ratios can be skewed in the context of in vitro fertilization. More broadly, our findings open the possibility for an early, non-invasive detection tool that could assist in identifying and addressing sex-related embryonic developmental abnormalities.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histidine and tyrosine residues are targets for SIRT6 ADP-ribosylation activity. 组氨酸和酪氨酸残基是SIRT6 adp核糖基化活性的靶标。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-03-04 DOI: 10.1098/rsob.250181

Johannes Gregor Matthias Rack, Evgeniia Prokhorova, Raffaella Lauro, Anna Georgina Kopasz, Domagoj Baretić, Roberto Raggiaschi, Jovana Mutabdzija, Dmitri V Filippov, Gyula Timinszky, Orsolya Leidecker, Ivan Ahel, Luca Palazzo

{"title":"Histidine and tyrosine residues are targets for SIRT6 ADP-ribosylation activity.","authors":"Johannes Gregor Matthias Rack, Evgeniia Prokhorova, Raffaella Lauro, Anna Georgina Kopasz, Domagoj Baretić, Roberto Raggiaschi, Jovana Mutabdzija, Dmitri V Filippov, Gyula Timinszky, Orsolya Leidecker, Ivan Ahel, Luca Palazzo","doi":"10.1098/rsob.250181","DOIUrl":"10.1098/rsob.250181","url":null,"abstract":"SIRT6, a highly conserved member of the sirtuin family, plays a critical role in diverse cellular processes, including gene regulation, DNA damage response and maintaining nuclear lamina integrity. These functions are essential in contexts such as differentiation, metabolic regulation, cancer development and ageing. Given the multifaceted influence of SIRT6 on cellular activities, there is an increasing interest in elucidating the regulatory mechanisms governing its enzymatic functions. SIRT6 exhibits two NAD+-dependent activities: deacetylation and ADP-ribosylation, with current research predominantly focusing on the former. However, the latter-its (ADP-ribosyl)transferase activity-remains underexplored, particularly concerning the specific amino acid targets it modifies and the (ADP-ribosyl)hydrolases that can reverse these modifications. In this study, we have utilized biochemical assays and proteomic techniques to investigate these aspects, revealing that SIRT6 transfers ADP-ribosyl moieties onto histidine and tyrosine residues. In addition, we reveal that the (ADP-ribosyl)hydrolase ARH3 has significant activity in erasing SIRT6-derived ADP-ribosylation in cells.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular spike waveform analysis reveals cell type-specific changes in the superior colliculus of fragile X mice. 细胞外尖峰波形分析揭示了脆性X小鼠上丘细胞类型特异性变化。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-02-25 DOI: 10.1098/rsob.250269

Gourav Sharma, Ashley L Russell, Karen G Dixon, Romina Fusha, Jason W Triplett

{"title":"Extracellular spike waveform analysis reveals cell type-specific changes in the superior colliculus of fragile X mice.","authors":"Gourav Sharma, Ashley L Russell, Karen G Dixon, Romina Fusha, Jason W Triplett","doi":"10.1098/rsob.250269","DOIUrl":"10.1098/rsob.250269","url":null,"abstract":"Sensory processing deficits are common in neurodevelopmental disorders (NDDs); however, we lack a full understanding of the circuits impacted. The superior colliculus (SC) is a sensorimotor region that directs complex behaviours, which recent work suggests is adversely impacted in NDDs. However, our understanding of cellular diversity in the SC lags in comparison to other regions, limiting our ability to parse circuit changes in NDDs. A goal of neuroscience has been to elucidate the diversity of neurons in the brain. Analysis of action potential shape in extracellular recordings has revealed subpopulations in several regions, allowing for insights into subtype-specific function in the intact brain. Here, we utilized semi-automated clustering methods to classify neurons in the mouse SC based on features of extracellularly recorded waveforms to identify five putative cell types. Secondary analysis of firing statistics and visual tuning properties supported cluster segregation. Interestingly, the proportions of units assigned to each cluster differed in a mouse model of fragile X syndrome (Fmr1-/y). Furthermore, we observed changes in waveform properties and firing statistics between genotypes in a subtype-specific manner. Taken together, these data add to our understanding of neuronal diversity in the SC and alterations of visual circuit organization and function in NDDs.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147318193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0