Open Biology最新文献_第2页

Mitochondrial-derived gene expression in hibernation: tissue-specific responses in the thirteen-lined ground squirrel. 冬眠中线粒体来源的基因表达：十三行地鼠的组织特异性反应。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-13 DOI: 10.1098/rsob.240255

Sarah Viola Emser, Eva Millesi

{"title":"Mitochondrial-derived gene expression in hibernation: tissue-specific responses in the thirteen-lined ground squirrel.","authors":"Sarah Viola Emser, Eva Millesi","doi":"10.1098/rsob.240255","DOIUrl":"10.1098/rsob.240255","url":null,"abstract":"Hibernation is a remarkable physiological adaptation in many mammals, characterized by prolonged torpor and profound metabolic suppression. Despite its importance, the molecular mechanisms regulating mitochondrial-derived gene expression during hibernation remain poorly understood. In this study, we analysed mitochondrial gene expression across multiple tissues of the hibernating thirteen-lined ground squirrel (Ictidomys tridecemlineatus) using publicly available RNA sequencing (RNA-seq) data. We assessed all known mitochondrial DNA-derived transcripts-including mitochondrial mRNAs, mitochondrial-derived peptides and proteins (MDPs), rRNAs, and long non-coding RNAs (lncRNAs)-in the liver, adrenal gland, three brain regions, and brown adipose tissue (BAT) across different hibernation states. Our findings reveal distinct tissue-specific expression patterns of mitochondrial transcripts. Differential expression was observed in three of the six tissues analysed (liver, adrenal gland, and BAT) while no significant changes were detected in the three brain regions. In tissues exhibiting differential expression, a consistent pattern emerged: lncRNAs such as Mdl1, Mdl1as, and lncCyb were generally upregulated, whereas mRNAs, including a novel transcript encoding the putative mitochondrial protein Rudel, were predominantly downregulated. These results provide new insights into mitochondrial gene regulation during hibernation and highlight tissue-specific adaptations at the level of mitochondrial gene expression.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"240255"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double trouble: cytosolic and nuclear IKKα in cancer. 双重麻烦：癌症的细胞质和核IKKα。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-06 DOI: 10.1098/rsob.240375

Kirsty Tinto, Margaret Cunningham, Robin Plevin

{"title":"Double trouble: cytosolic and nuclear IKKα in cancer.","authors":"Kirsty Tinto, Margaret Cunningham, Robin Plevin","doi":"10.1098/rsob.240375","DOIUrl":"10.1098/rsob.240375","url":null,"abstract":"IκB kinase alpha (IKKα) is a serine/threonine kinase originally known for its role in nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signalling, which integrates inflammatory processes and cancer. IKKα can function within the IKK complex in canonical NF-κB signalling, alongside its homologous family member, IKKβ, and regulatory subunit IKKγ (NEMO). However, a key role for IKKα is its ability to promote non-canonical NF-κB signalling. Additionally, the dynamic ability of IKKα to shuttle between the cytosol and nucleus, mediates NF-κB-independent effects which further its role in inflammation and tumour progression. More recently, an endosomal-generated, nuclear-active IKKα isoform, p45-IKKα has been discovered and implicated in cancer and chemoresistance. This review focuses on current knowledge of the complex and intricate roles of nuclear and cytosolic IKKα in promoting tumour progression. By highlighting the molecular roles of IKKα in several cancer subtypes, and its integral roles in many of the hallmarks of cancer throughout this review, we highlight the therapeutic potential of IKKα as a future anti-cancer drug target.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"240375"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain amino acid sensing for organismal amino acid homeostasis. 脑氨基酸感知对机体氨基酸稳态的影响。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-06 DOI: 10.1098/rsob.250092

Anthony H Tsang, Liubou Samson, Clemence Blouet

{"title":"Brain amino acid sensing for organismal amino acid homeostasis.","authors":"Anthony H Tsang, Liubou Samson, Clemence Blouet","doi":"10.1098/rsob.250092","DOIUrl":"10.1098/rsob.250092","url":null,"abstract":"Amino acids are essential for normal physiological functions, and disruptions in their circulating concentrations are implicated in the pathophysiology of various diseases. Therefore, understanding the mechanisms that regulate circulating amino acid levels in normal physiology is of critical importance. Evidence indicates that in healthy mammals, post-absorptive circulating levels of essential amino acids are maintained within a range that varies little from day to day or following bidirectional changes in dietary protein intake. This suggests the presence of homeostatic control mechanisms. Here, we propose a conceptual framework for the homeostatic regulation of essential amino acid availability, emphasizing the role of the brain in generating feedback controls to restore baseline levels acutely after a meal and during chronic changes in dietary protein intake. We review current evidence supporting brain amino acid sensing as a component of this regulatory system, integrating peripheral and central signals to modulate dietary protein intake and peripheral amino acid metabolism. We highlight major knowledge gaps regarding the specific neural circuits, molecular mechanisms and physiological outcomes of brain amino acid sensing. Future inquiry using the proposed framework and addressing these gaps will significantly enhance our understanding of the pathways involved in the maintenance of circulating amino acid availability and the regulation of lean mass in health, disease states or in response to therapeutic strategies for metabolic diseases.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"250092"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional disruption of oxytocin projections participates atypical social and anxiety-like behaviours in BTBR mouse model of autism. 在BTBR自闭症小鼠模型中，催产素投射的功能破坏参与了非典型社交和焦虑样行为。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-27 DOI: 10.1098/rsob.240387

Yuki Higuchi, Akihiko Ozawa, Ryuki Kobayashi, Toshihiro Konno, Hiroyuki Arakawa

{"title":"Functional disruption of oxytocin projections participates atypical social and anxiety-like behaviours in BTBR mouse model of autism.","authors":"Yuki Higuchi, Akihiko Ozawa, Ryuki Kobayashi, Toshihiro Konno, Hiroyuki Arakawa","doi":"10.1098/rsob.240387","DOIUrl":"https://doi.org/10.1098/rsob.240387","url":null,"abstract":"Oxytocin (OXT) neurons in the paraventricular nucleus of the hypothalamus (PVN), which send projections to the medial amygdala (MeA) and the bed nucleus of the stria terminalis (BnST), are implicated in regulation of prosocial-emotional behaviours and abnormalities resembling autism spectrum disorders (ASD). Compared with standard C57BL6J (B6) mice, BTBR mice, a behaviour-based ASD model, exhibited decreased densities of OXTPVN neurons and attenuated OXT neuronal responses to a social encounter. OXT receptor mRNA expressions in the MeA and BnST as a response to a social encounter were blunted in BTBR mice. OXT promoter retrograde viral tracing revealed that the OXTPVN→BnST projections were defective in those BTBR mice. Thus, chemogenetic excitation of OXTPVN→MeA neurons using OXT promoter adeno-associated viruses (AAV) enhanced anxiety-like behaviour and facilitated social investigation in both strains, while excitation of OXTPVN→BnST neurons attenuated anxiety-like behaviour along with social investigation in B6 mice and failed to induce a change in their socio-emotional behaviours in BTBR mice. Altogether, OXT circuits serve as a key regulator for socio-emotional behaviour; MeA-OXT projection facilitates social investigation and anxiety-like behaviour, while BnST-OXT projection conversely attenuates these behaviours; hence a defect of the OXTPVN→BnST circuits contributes to the development of ASD-like social phenotypes in BTBR mice.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"240387"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective loss of ATP carriers in favour of SLC25A43 orthologues in metamonad mitochondria adapted to anaerobiosis. 在适应厌氧的异单胞体线粒体中，ATP载体的选择性丧失有利于SLC25A43同源物。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-13 DOI: 10.1098/rsob.240202

Natalia Janowicz, Vít Dohnálek, Justyna Zítek, Priscila Peña-Diaz, Eva Pyrihová, Martin S King, Michaela Husová, Vojtěch Žárský, Edmund Kunji, Alena Zikova, Vladimír Hampl, Pavel Dolezal

{"title":"Selective loss of ATP carriers in favour of SLC25A43 orthologues in metamonad mitochondria adapted to anaerobiosis.","authors":"Natalia Janowicz, Vít Dohnálek, Justyna Zítek, Priscila Peña-Diaz, Eva Pyrihová, Martin S King, Michaela Husová, Vojtěch Žárský, Edmund Kunji, Alena Zikova, Vladimír Hampl, Pavel Dolezal","doi":"10.1098/rsob.240202","DOIUrl":"10.1098/rsob.240202","url":null,"abstract":"Metamonada is a eukaryotic supergroup of free-living and parasitic anaerobic protists. Their characteristic feature is the presence of highly reduced mitochondria that have lost the ability to produce ATP by oxidative phosphorylation and in some cases even by substrate phosphorylation, with all ATP being imported from the cytosol. Given this striking difference in cellular ATP metabolism when compared to aerobic mitochondria, we studied the presence of mitochondrial carrier proteins (MCPs) mediating the transport of ATP across the inner mitochondrial membrane. Our bioinformatic analyses revealed remarkable reduction of MCP repertoire in Metamonada with striking loss of the major ADP/ATP carrier (AAC). Instead, nearly all species retained carriers orthologous to human SLC25A43 protein, a little-characterized MCP. Heterologous expression of metamonad SLC25A43 carriers confirmed their mitochondrial localization, and functional analysis revealed that SLC25A43 orthologues represent a distinct group of ATP transporters, which we designate as ATP-importing carriers (AIC). Together, our findings suggest that AIC facilitate the ATP import into highly reduced anaerobic mitochondria, compensating for their diminished or absent energy metabolism.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"240202"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of unique mitotic mechanisms in Paradiplonema papillatum. 乳突旁体独特有丝分裂机制的发现。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-06 DOI: 10.1098/rsob.250096

Bungo Akiyoshi, Drahomíra Faktorová, Julius Lukeš

{"title":"Discovery of unique mitotic mechanisms in Paradiplonema papillatum.","authors":"Bungo Akiyoshi, Drahomíra Faktorová, Julius Lukeš","doi":"10.1098/rsob.250096","DOIUrl":"10.1098/rsob.250096","url":null,"abstract":"Diplonemids are highly diverse and abundant marine plankton with significant ecological importance. However, little is known about their biology, even in the model diplonemid Paradiplonema papillatum whose genome sequence is available. Examining the subcellular localization of proteins using fluorescence microscopy is a powerful approach to infer their putative function. Here, we report a plasmid-based method that enables YFP-tagging of a gene at the endogenous locus. By examining the localization of proteins whose homologs are involved in chromosome organization or segregation in other eukaryotes, we discovered several notable features in mitotically dividing P. papillatum cells. Cohesin is enriched on condensed interphase chromatin. During mitosis, chromosomes organize into two rings (termed mitotic rings herein) that surround the elongating nucleolus and align on a bipolar spindle. Homologs of chromosomal passenger complex components (INCENP, two Aurora kinases and KIN-A), a CLK1 kinase, meiotic chromosome axis protein SYCP2L1, spindle checkpoint protein Mad1 and microtubule regulator XMAP215 localize in between the two mitotic rings. In contrast, a Mad2 homolog localizes near basal bodies as in trypanosomes. By representing the first molecular characterization of mitotic mechanisms in P. papillatum and raising many questions, this study forms the foundation for dissecting mitotic mechanisms in diplonemids.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"250096"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using diapause as a platform to understand the biology of dormancy. 用滞育作为一个平台来理解休眠的生物学。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-20 DOI: 10.1098/rsob.250104

Nathaniel A Sweet, Chi-Kuo Hu

{"title":"Using diapause as a platform to understand the biology of dormancy.","authors":"Nathaniel A Sweet, Chi-Kuo Hu","doi":"10.1098/rsob.250104","DOIUrl":"10.1098/rsob.250104","url":null,"abstract":"Diapause is a fascinating form of biological dormancy that is employed by a broad array of animals as a survival strategy to endure adverse environmental conditions. This unique dormant state can suspend organismal development until a more favourable condition arises, giving the species the greatest chance to survive as a whole. Remarkably, while following the same principle of suspending development, diapause exists in different forms and can occur at various stages before reaching the adult form. Functionally, with multiple evolutionary origins across the animal kingdom, diapause demonstrates the ability to respond to diverse environmental challenges while converging to maintain the same core function of suspending development. At the physiological level, these different diapause states share a similar metabolic adaptation to conserve resources and energy throughout dormancy. Underneath, the same genes have been repeatedly identified as regulators and effectors of diapause at different developmental stages in both invertebrates and vertebrates. This suggests the presence of a conserved molecular programme comprised of the same set of key genes repeatedly reprogrammed and utilized at the core of diapause. The knowledge of diapause from the organismal to molecular levels, together, should serve as a useful window to better understand the biology of dormancy.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"250104"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional regulation as a dose-dependent process: insights from transcription factor tuning. 转录调节作为一个剂量依赖的过程：从转录因子调节的见解。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-06 DOI: 10.1098/rsob.240328

Gemma Noviello

引用次数: 0

The curious family of cytochrome P450 4F fatty acid ω-hydroxylases: recent developments in their function and relevance for human health. 细胞色素P450 4F脂肪酸ω-羟化酶的奇特家族：其功能及其与人类健康的相关性的最新进展。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-27 DOI: 10.1098/rsob.250115

Brisa Caroline Alves Chagas, Huiting Jia, Bjoern Brixius, Simone Brixius-Anderko

引用次数: 0

Developmental remodelling of Drosophila flight muscle sarcomeres: a scaled myofilament lattice model based on multiscale morphometrics. 果蝇飞行肌肌节的发育重塑：基于多尺度形态计量学的比例肌丝晶格模型。

IF 3.6 3区生物学

Open Biology Pub Date : 2025-08-01 Epub Date: 2025-08-13 DOI: 10.1098/rsob.250182

Péter Görög, Tibor Novák, Tamás F Polgár, Péter Bíró, Adél Gutheil, Csaba Kozma, Tamás Gajdos, Krisztina Tóth, Alexandra Tóth, Miklós Erdélyi, József Mihály, Szilárd Szikora

{"title":"Developmental remodelling of Drosophila flight muscle sarcomeres: a scaled myofilament lattice model based on multiscale morphometrics.","authors":"Péter Görög, Tibor Novák, Tamás F Polgár, Péter Bíró, Adél Gutheil, Csaba Kozma, Tamás Gajdos, Krisztina Tóth, Alexandra Tóth, Miklós Erdélyi, József Mihály, Szilárd Szikora","doi":"10.1098/rsob.250182","DOIUrl":"10.1098/rsob.250182","url":null,"abstract":"The indirect flight muscle is a widely used model for studying sarcomere structure and muscle development due to its extremely regular architecture. Nevertheless, precise measurement of the basic sarcomeric parameters remains a challenge even in this greatly ordered tissue. In this study, we identified several factors affecting measurement reliability and developed a software tool for precise, high-throughput measurement of sarcomere length and myofibril width. The accuracy of this new tool was validated against simulated images and blinded manual measurements. To extend the scope of this morphometric analysis to the sub-sarcomeric scale, we used electron and super-resolution microscopy to quantify myofilament number and filament length during myofibrillogenesis. Our findings revealed the dynamics of thin and thick filament elongation, as well as the addition of myofilaments at the sarcomere periphery during myofibrillogenesis. We precisely measured the dimensions of the Z-disc, I-band and H-zone during development, enabling us to construct refined models of sarcomere growth at the level of individual myofilaments, providing a spatial framework for interpreting nanoscopic localization data. These models deepen our understanding of sarcomere growth and lay the groundwork for future studies on the molecular mechanisms driving myofilament elongation and assembly.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 8","pages":"250182"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0