Oral Therapeutics and Pharmacology最新文献

{"title":"A simple and useful method for evaluating biocompatibility of titanium alloy with heterotopic bone induced by demineralized bone matrix (DBM) pellet in mice","authors":"M. Arai, T. Nagasawa, Yoshihiko Tsunashima, Tomohiro Matsuda, H. Kawamura, M. Mogi, A. Togari","doi":"10.11263/JSOTP1982.22.117","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.117","url":null,"abstract":"Titanium (Ti) and Ti alloys are widely used for dental materials. Recently, an aluminium and vanadium free titanium alloy, Ti-29Nb-13Ta-4.6Zr alloy, was developed. In the present study, we used the demineralized bone matrix (DBM) pellet for evaluating biocompatibility of Ti, Ti-6A1-4V alloy (the most widely used alloy) and Ti-29Nb-13Ta-4.6Zr alloy. The DBM pellet made of mouse long bones was implanted on the dorsal muscle of mice under ether anesthesia. After the DBM pellet had been implanted for 4 weeks, the expression of mRNA for osteoblastic and osteoclastic phenotypes and mineralization were observed, suggesting that the DBM could heterotopically induce bone. The DBM pellet combined with a disk of Ti, Ti-6A1-4V alloy and Ti-29Nb-13Ta-4.6Zr alloy was implanted in the mouse in the same way. Four weeks after the implantation, the calcium contents accumulated in the DBM pellet with Ti, Ti-6A1-4V alloy and Ti-29Nb-13Ta-4.6Zr alloy were 36.6±8.0 p g, 50.4± 13.3 p g and 75.6± 16.9 p g, respectively. A significant difference was observed between Ti and Ti-29Nb-13Ta-4.6Zr alloy (p<0.05). This result seemed to suggest the increase of the mineralization in DBM-induced heterotopic bone by Ti-29Nb-13Ta-4.6Zr alloy, because Ti particles showed no toxicities in mouse osteoblastic cells, in mouse bone marrow cells, and in isolated rabbit osteoclasts. These findings suggested that Ti-29Nb-13Ta-4.6Zr alloy might be a higher biocompatible material with bone tissue, and showed that the heterotopic bone induced by the DBM pellet in mice could be a simple and useful method for evaluating biocompatibility of titanium alloys.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"117-126"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of differentiation in leukemia THP-1 cells treated with adenosine and ATP","authors":"Y. Azuma, Kyoko Watanabe, Sinya Shirasu, M. Daito, K. Ohura","doi":"10.11263/JSOTP1982.22.127","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.127","url":null,"abstract":"Adenosine and ATP have been implicated in the regulation of inflammatory responses. Furthermore, adenosine and ATP affect cell growth, cell differentiation, and apoptosis. The aim of this study was to evaluate differentiation inducing abilities of adenosine and ATP on human monocytic leukemia THP-1 cells. Adenosine at 10-3 M markedly induced the differentiation in leukemia THP-1 cells as assessed by evaluating the expression of CDllb. Similarly, ATP at 10-3 M significantly induced the differentiation in THP-1 cells. Simultaneously, adenosine at 10-3 M and ATP at 10-3 M significantly inhibited the cell number in THP-1 cells. Next, we have evaluated abilities of adenosine and ATP on THP-1 cells differentiated by differentiation inducers all-trans retinoic acid (ATRA), 1,25-dihydroxy-vitamin D3 (VD3) and phorbol 12-myristate 13-acetate (PMA). Interestingly, adenosine at 10-3 M significantly potentiated ATRA-induced CDllb expression. In addition, ATRA significantly increased adenosine-induced CDllb expression. Similarly, ATP at 10-3 M significantly potentiated ATRA-induced CDllb expression. In addition, ATRA potentiated ATP-induced CDllb expression. In contrast, VD3 and PMA further increased the expression of CDllb in the presence of adenosine of ATP, respectively. These results suggest that adenosine and ATP may induce the differentiation in human leukemia THP-1 cells, and may further increase the ATRA-induced differentiation in THP-1 cells.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"127-135"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenosine and ATP affect LPS-induced cytokine production by macrophages","authors":"Kyoko Watanabe, Y. Azuma, S. Shirasu, M. Daito, K. Ohura","doi":"10.11263/JSOTP1982.22.105","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.105","url":null,"abstract":"Macrophages are essential for controlling the majority of infections, and are mediators of natural immunity. During infection, lipopolysaccharide (LPS) stimulates macrophages to produce pro-inflammatory cytokines. Adenosine and ATP released into the extracellular space by immunological stimuli have been shown to regulate various immune functions. We evaluate immunological effects of adenosine and ATP on the production of cytokines related to inflammation and Thl/Th2 balance by rat peritoneal macrophages. Adenosine and ATP respectively increased the production of IL-10 without affecting the production of IL-1 ƒÀand IL-12 by macrophages. In addition, adenosine and ATP prevented the production of IL-1 iS and IL-12 by LPSstimulated macrophages. In contrast, adenosine inhibited IL-10 production by LPSstimulated macrophages, whereas ATP potentiated IL-10 production by LPS-stimulated macrophages. These results suggest that conditions related to increased adenosine and/or ATP may play an important role in .a wide range of immune reactions including the Thl and Th2 immune response.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"105-110"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DP receptor signaling increased LPS-induced TNF-α production by macrophages","authors":"Kiyoshi Daito, Y. Azuma, Kyoko Watanabe, S. Shirasu, Mikio Kato, M. Daito, K. Ohura","doi":"10.11263/JSOTP1982.22.153","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.153","url":null,"abstract":"","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"153-159"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations of nuclear localization of transcription factor c-Jun in brain of diabetic rats with hyperglycemia","authors":"Kiyoshi Daito, Y. Azuma, Kyoko Watanabe, S. Shirasu, Mikio Kato, M. Shinohara, M. Daito, K. Ohura","doi":"10.11263/JSOTP1982.22.136","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.136","url":null,"abstract":"De novo protein synthesis in eukaryotes is mainly controlled at the level of gene transcription by transcription factors in the nucleus. In this communication, the expression and nuclear localization of the activator protein-1 (AP1) family member c-Jun in nuclear and cytosolic fractions of brain and retina from streptozotocin (STZ)-injected diabetic rats were determined in order to evaluate the possibility that transportation of AP1 family members from the cytoplasm to the nucleus could play a role in diabetes. Immunoblotting assays with a specific antibody revealed a much higher expression of immunoreactive c-Jun in nuclear fractions than in cytosolic fractions of the cerebral cortex, hippocampus, striatum and cerebellum after diabetes induction by administration of STZ. In contrast, administration of STZ failed to affect the nuclear localization of c-Jun detected in the hypothalamus, midbrain, medulla-pons and retina. These results suggest that the STZ-injected diabetic rat shows differential alterations in nuclear localization of c-Jun protein in the brain and retina.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"136-144"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible mechanisms underlying acceleration of proliferation by 15-deoxy-Δ12, 14-prostaglandin J2 and the precursors in human T acute lymphoblastic leukemia cell line CCRF-CEM","authors":"Y. Azuma, Kyoko Watanabe, M. Date, S. Shirasu, M. Daito, K. Ohura","doi":"10.11263/JSOTP1982.22.160","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.160","url":null,"abstract":"","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"57 1","pages":"160-168"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of prototype low viscid toothpaste containing cetylpyridinium chloride with interproximal brushes on gingivitis","authors":"T. Nomura, Takashi Yanagisawa, M. Yuasa, K. Kamo, Yoshio Saito, Noriaki Yoshida, T. Arai","doi":"10.11263/JSOTP1982.22.111","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.111","url":null,"abstract":"There have been many applications of toothpastes and gargles containing antibiotic ingredients to periodontal treatment. Cetylpyridinium chloride (CPC) is one such anti-plaque ingredient with a content of 0.05%. This study evaluated the effects of interproximal brushes with a prototype low viscid toothpaste containing 0.05% of CPC on slight periodontitis and gingivitis at interproximal areas. The subjects were 60 patients who had not received general and/or local antibiotics or periodontal therapy for at least 3 months prior to this study. They had at least 1 interproximal area where interproximal brushes could be inserted on the maxillar or mandibular incisors to second premolars without prosthesis. Sampling sites were interproximal areas in each subject. The subjects in the experimental group were instructed as to brushing method and used interproximal brushes with the prototype low viscid toothpaste containing 0.05% of CPC. The other subjects in the placebo group used a prototype low viscid toothpaste without CPC and were given the same instructions as the experimental group of subjects. Mondified gingival index (MGI), modified plaque index (MPI) , amount of gingival cervical fluid (GCF) , probing depth (PD), clinical attachment level (CAL) and ratio of subgingival microflora were measured at baseline, and after 1 month and 2 months. The data from 23 patients in the experimental group and 25 patients in the placebo group were adopted in this study. MGI, MPI, GCF and PD in both groups showed a significant reduction with time. GCF and PD in the experimental group were significantly lower than those of the placebo group at 1 month. CAL in both groups did not change at any period. Ratio of cocci increased with time in both groups, accordingly rods decreased with time. Spirochetes and motile rods were scarcely detected at every period. These results suggested that the use of low viscid toothpaste containing 0.05% of cetypyridinium chloride with interproximal brushes could quickly improve gingivitis.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"111-116"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of pathological fracture secondary to osteomyelitis of the mandible","authors":"K. Asada, S. Tochihara, S. Yamachika, A. Nakayama, K. Ishibashi","doi":"10.11263/JSOTP1982.22.69","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.69","url":null,"abstract":"","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Change of subgingival bacterial flora and level of drug concentration in gingiva of periodontal patients systemically administered azithromycin","authors":"Naoya Shibukawa, K. Gomi, F. Iino, M. Kanazashi, J. Suzuki, T. Ohshima, N. Maeda, T. Arai","doi":"10.11263/JSOTP1982.22.83","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.83","url":null,"abstract":"Azithromycin is an azalide antibiotic, which has a strong effect on a wide variety of oral bacteria. It is taken up by phagocytes and is released over a long period in the inflamed tissue. This study investigated the clinical and microbiological effects, and measured the drug concentration in gingival of periodontal patients systemically administered azithromycin. The subjects were 26 adults diagnosed with periodontitis and given azithromycin (zithromacðF) 500 mg once daily for 3 days. Clinical parameters such as PD, GI, BOP and GCF were examined at days 0, 7, and 14, and subgingival plaque was collected by paper points at days 0, 4, 7 and 14. The total number of cultivable bacteria was counted and six of the periodontopathic bacteria •Porphyromonas gingivalis, Actino bacillus actinomysetemcomitans, Bacteroides forsythus, Prevotella intermedia, Prevo tella nigresence, and Toreponema denticola•were assessed by the PCR method. The lining gingiva of periodontal pocket was collected by pocket curettage at days 4 and 7. The azithromycin concentration was measured by agar diffusion bioassay. The total number of bacteria was significantly decreased at days 4 and 7, but was slightly increased at day 14. Continuous reduction of the six bacteria was recognized until day 14 by PCR. These bacteria were not detected at day 14 without Porphyro monas gingivalis. This result might be due to improvement of anaerobic condition caused by pocket reduction. The azithromycin concentration in the lining gingival tissues of periodontal pocket was 2.92•}1.97ƒÊg/g at day 4 and 1.27•}0.95ƒÊg/g at day 7. Half the drug concentration was still detected at 7 days after the first administration in inflamed gingiva. These results indicate that azithromycin may be a useful adjunct agent for adult periodontitis. In the future, we will consider a new periodontal therapy using this behavior of azithromycin.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"74 1","pages":"83-90"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A clinical evaluation of Azithromycin and questionnaire survey in patients with acute dental infection","authors":"N. Terakado, S. Shintani, S. Hara, H. Shinohara, Junya Yano, H. Kayahara, K. Nakashiro, S. Hino, H. Hamakawa","doi":"10.11263/JSOTP1982.22.76","DOIUrl":"https://doi.org/10.11263/JSOTP1982.22.76","url":null,"abstract":"The efficacy of azithromycin as a chemoprophylactic agent for acute dental infection was assessed, and a questionnaire survey about the impression of azithromycin was conducted. METHODS: Eighty-three patients were given 500 mg of azithromycin for three days. Seven days after the onset of the treatment, we evaluated the clinical symptoms and collected the questionnaires. RESULTS: Clinical efficacy was observed in 89.2% of the patients, and that by questionnaire was 86.7%. Diarrhea as a side effect was seen in two patients. In the questionnaire, 79% of patients noted its effect from the second to fourth day after the medication. Although half of the patients did not care about the convenience, of taking the medication once a day, which is one of the advantages of azithromycin, most of the patients were satisfied with its efficacy. Furthermore, 72.3% of patients considered azithromycin to be significantly more acceptable compared with other antibiotics. CONCLUSION : Azithromycin is a useful therapeutic regimen for the treatment of acute dental infections.","PeriodicalId":19590,"journal":{"name":"Oral Therapeutics and Pharmacology","volume":"22 1","pages":"76-82"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}