Y. Azuma, Kyoko Watanabe, Sinya Shirasu, M. Daito, K. Ohura
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引用次数: 0
摘要
腺苷和ATP参与了炎症反应的调节。此外,腺苷和ATP影响细胞生长、细胞分化和细胞凋亡。本研究旨在探讨腺苷和ATP对人单核细胞白血病THP-1细胞的诱导分化能力。通过评价CDllb的表达来评估10-3 M腺苷显著诱导白血病THP-1细胞分化。同样,ATP在10-3 M时显著诱导THP-1细胞分化。同时,10-3 M的腺苷和10-3 M的ATP显著抑制THP-1细胞的数量。接下来,我们评估了腺苷和ATP对由分化诱导剂全反式维甲酸(ATRA)、1,25-二羟基维生素D3 (VD3)和12-肉豆酸酯13-乙酸佛波酯(PMA)分化的THP-1细胞的能力。有趣的是,10-3 M的腺苷显著增强了atra诱导的CDllb表达。此外,ATRA显著增加了腺苷诱导的CDllb表达。同样,10-3 M ATP显著增强atra诱导的CDllb表达。此外,ATRA增强了atp诱导的CDllb表达。相比之下,VD3和PMA分别在ATP腺苷存在下进一步增加CDllb的表达。提示腺苷和ATP可诱导人白血病THP-1细胞分化,并可进一步增强atra诱导的THP-1细胞分化。
Induction of differentiation in leukemia THP-1 cells treated with adenosine and ATP
Adenosine and ATP have been implicated in the regulation of inflammatory responses. Furthermore, adenosine and ATP affect cell growth, cell differentiation, and apoptosis. The aim of this study was to evaluate differentiation inducing abilities of adenosine and ATP on human monocytic leukemia THP-1 cells. Adenosine at 10-3 M markedly induced the differentiation in leukemia THP-1 cells as assessed by evaluating the expression of CDllb. Similarly, ATP at 10-3 M significantly induced the differentiation in THP-1 cells. Simultaneously, adenosine at 10-3 M and ATP at 10-3 M significantly inhibited the cell number in THP-1 cells. Next, we have evaluated abilities of adenosine and ATP on THP-1 cells differentiated by differentiation inducers all-trans retinoic acid (ATRA), 1,25-dihydroxy-vitamin D3 (VD3) and phorbol 12-myristate 13-acetate (PMA). Interestingly, adenosine at 10-3 M significantly potentiated ATRA-induced CDllb expression. In addition, ATRA significantly increased adenosine-induced CDllb expression. Similarly, ATP at 10-3 M significantly potentiated ATRA-induced CDllb expression. In addition, ATRA potentiated ATP-induced CDllb expression. In contrast, VD3 and PMA further increased the expression of CDllb in the presence of adenosine of ATP, respectively. These results suggest that adenosine and ATP may induce the differentiation in human leukemia THP-1 cells, and may further increase the ATRA-induced differentiation in THP-1 cells.