Ophthalmology. Retina最新文献

{"title":"Clinical Evaluation of a Novel CRISPR-Cas12a-Based RID-MyC Assay for the Diagnosis of Fungal Endophthalmitis","authors":"Hanith Raj Deivarajan MSc ,&nbsp;Prabhakara Sethupathy R MSc ,&nbsp;Vignesh Elamurugan MBBS ,&nbsp;Akshayaa VS MSc ,&nbsp;Reega P MSc ,&nbsp;Dharani Chelliah MSc ,&nbsp;Hari Vignesh S MSc ,&nbsp;Elakkiya Nandhini GR MSc ,&nbsp;Kanmani M MSc ,&nbsp;Dharsini Nandhakumar BE ,&nbsp;Karvannan Sevugamurthi MSc ,&nbsp;Saravanan VR MBBS, DNB ,&nbsp;Anuradha K MBBS, FRCS ,&nbsp;Parag K. Shah MBBS, DNB ,&nbsp;Ram Rammohan PhD ,&nbsp;Abhishek Nair MBBS ,&nbsp;Krutin Shah MBBS ,&nbsp;Anju Jose MBBS, DNB ,&nbsp;Jaishree Pandian PhD ,&nbsp;Lalitha Prajna MBBS ,&nbsp;Siddharth Narendran MBBS","doi":"10.1016/j.oret.2024.11.003","DOIUrl":"10.1016/j.oret.2024.11.003","url":null,"abstract":"<div><h3>Objective</h3><div>This study evaluated the RID-MyC (Rapid Identification of Mycoses using clustered regularly interspaced short palindromic repeats [CRISPR]) assay, a CRISPR/Cas12a-based diagnostic tool, for its efficacy in diagnosing fungal endophthalmitis (FE) compared with panfungal polymerase chain reaction (PCR) and culture methods.</div></div><div><h3>Design</h3><div>A comparative cross-sectional study assessing the performance of the RID-MyC assay against established diagnostic modalities for FE.</div></div><div><h3>Subjects</h3><div>The study included 133 intraocular samples from 117 patients with suspected microbial endophthalmitis.</div></div><div><h3>Methods</h3><div>The study compared the sensitivity, specificity, positive predictive value, and negative predictive value of the RID-MyC assay against panfungal PCR and culture. The limit of detection for <em>Aspergillus flavus</em> and <em>Candida albicans</em> was determined for both RID-MyC and panfungal PCR across 3 different media: nuclease-free water, aqueous humor, and vitreous humor. Discrepancy analysis was conducted for discordant results, incorporating clinical outcomes and responses to antifungal treatment.</div></div><div><h3>Main Outcome Measures</h3><div>The study primarily assessed the sensitivity, specificity, positive predictive value, and negative predictive value for clinical samples. Time to diagnosis was also evaluated.</div></div><div><h3>Results</h3><div>The RID-MyC assay demonstrated a sensitivity of 88.24% (95% confidence interval [CI], 63.56%–98.54%) and specificity of 93.1% (95% CI, 86.86%–96.98%), with positive predictive value and negative predictive value of 65.22% (95% CI, 48.45%–78.91%) and 98.18% (95% CI, 93.62%–99.50%), respectively. Discrepancy analysis enhanced sensitivity to 90.48% (95% CI, 69.62%–98.83%) and specificity to 96.43% (95% CI, 91.11%–99.02%). The RID-MyC assay was 10- to 1000-fold more sensitive than panfungal PCR in detecting <em>A. flavus</em> and <em>C. albicans</em> in intraocular specimens. The time to diagnosis with the RID-MyC assay was consistently &lt;2 hours.</div></div><div><h3>Conclusions</h3><div>The RID-MyC assay may advance the rapid and precise diagnosis of FE, with possible relevance to other invasive fungal conditions.</div></div><div><h3>Financial Disclosure(s)</h3><div>Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.</div></div>","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Pages 444-452"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Macular Function: Should We Move Beyond Pragmatic Measures of Visual Acuity?","authors":"Marion R. Munk MD, PhD,&nbsp;Eleonora M. Lad MD, PhD,&nbsp;Usha Chakravarthy MD, PhD","doi":"10.1016/j.oret.2025.01.006","DOIUrl":"10.1016/j.oret.2025.01.006","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Pages 409-411"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy of B-Scan Ultrasonography in Detecting Vitreoretinal Pathology after Open-Globe Injury","authors":"Mahsaw Mansoor MD,&nbsp;Matthew S. Hunt MD,&nbsp;Elaine M. Binkley MD,&nbsp;Timothy M. Boyce MD,&nbsp;Ian C. Han MD,&nbsp;Elliott H. Sohn MD,&nbsp;Stephen R. Russell MD,&nbsp;H. Culver Boldt MD,&nbsp;Jonathan F. Russell MD, PhD","doi":"10.1016/j.oret.2024.10.028","DOIUrl":"10.1016/j.oret.2024.10.028","url":null,"abstract":"<div><h3>Purpose</h3><div>To assess the diagnostic accuracy of B-scan ultrasonography (echography) after open-globe injury (OGI) repair in detecting vitreoretinal pathology, as confirmed by intraoperative inspection during subsequent pars plana vitrectomy (PPV).</div></div><div><h3>Design</h3><div>Retrospective, single-center, consecutive case series.</div></div><div><h3>Participants</h3><div>Patients with OGI treated at the University of Iowa Hospitals and Clinics from February 2018 through December 2023 who underwent OGI repair and had at least 1 B-scan performed postrepair but before subsequent PPV.</div></div><div><h3>Methods</h3><div>B-scans were performed by an experienced echographer and reviewed by the managing vitreoretinal surgeon for the presence of vitreous hemorrhage (VH), retinal tear (RT), retinal detachment (RD), choroidal detachment, and vitreoretinal incarceration. B-scan findings were compared with findings on direct inspection during PPV, which served as the gold standard.</div></div><div><h3>Main Outcome Measures</h3><div>Sensitivity, specificity, and positive/negative predictive value of B-scan findings.</div></div><div><h3>Results</h3><div>The study included 62 eyes of 61 patients, predominantly with severe OGIs (mean presenting logarithm of the minimum angle of resolution visual acuity of 2.52 ± 0.41; 75% with an Ocular Trauma Score of 1 or 2). B-scan had excellent diagnostic accuracy for VH, but, for every other type of vitreoretinal pathology, there were significant false positives, false negatives, or both. B-scan sensitivity was particularly low for vitreoretinal incarceration (11%), RT (32%), and RD (78%).</div></div><div><h3>Conclusions</h3><div>This study identified much lower diagnostic accuracy of B-scan ultrasonography after OGI for all vitreoretinal pathologies except VH compared with previous, smaller studies that reported perfect accuracy (100% sensitivity and specificity). Ultrasonography provides useful clinical information but should not be solely relied upon to diagnose or rule out severe vitreoretinal pathology that may prompt vitreoretinal referral or PPV after OGI.</div></div><div><h3>Financial Disclosure(s)</h3><div>Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.</div></div>","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Pages 453-459"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: Cai et al.: Similar risk of kidney failure among patients with blinding diseases who receive ranibizumab, aflibercept, and bevacizumab: an observational health data sciences and informatics network study (Ophthalmol Retina. 2024;8:733-743)","authors":"Flora Lum MD","doi":"10.1016/j.oret.2025.01.013","DOIUrl":"10.1016/j.oret.2025.01.013","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Pages e39-e40"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Optic Disc Pit with Maculopathy","authors":"Savithiri Palanivel DOMS, DNB,&nbsp;Anand Rajendran FRCS, DNB","doi":"10.1016/j.oret.2024.08.023","DOIUrl":"10.1016/j.oret.2024.08.023","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Page e43"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymptomatic Juvenile White Retinal Arterioles","authors":"Colya N. Englisch ,&nbsp;Claus Englisch MD","doi":"10.1016/j.oret.2024.09.003","DOIUrl":"10.1016/j.oret.2024.09.003","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Page e44"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142471483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Area Deprivation Index and Retinopathy of Prematurity","authors":"Jalin A. Jordan BA ,&nbsp;Charis Turner MD ,&nbsp;Isdin Oke MD ,&nbsp;Julius T. Oatts MD","doi":"10.1016/j.oret.2024.12.023","DOIUrl":"10.1016/j.oret.2024.12.023","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Pages 495-498"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: Alshaikhsalama et al.: Association between obstructive sleep apnea and age-related macular degeneration development and progression (Ophthalmol Retina. 2024 Dec 9:S2468-6530(24)00580-3. doi: 10.1016/j.oret.2024.12.004. Online ahead of print.)","authors":"Isdin Oke MD, MPH ,&nbsp;Kenshiro Fuse MD ,&nbsp;Nils Krüger MD ,&nbsp;Vinicius A. Schoeps MD, MPH ,&nbsp;Camila R. Guetter MD, MPH","doi":"10.1016/j.oret.2025.02.004","DOIUrl":"10.1016/j.oret.2025.02.004","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Page e41"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electron Microscopy to Confirm Rhegmatogenous Cause of Retinal Detachments","authors":"Shivesh Varma MBBS, FRANZCO ,&nbsp;Chris Van Vliet MBChB, FRCPA ,&nbsp;Chandrakumar Balaratnasingam MD, PhD","doi":"10.1016/j.oret.2024.09.014","DOIUrl":"10.1016/j.oret.2024.09.014","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Page e48"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obliterative Vasculitis as the Presenting Sign of Systemic Lupus Erythematosus in a 12-Year-Old","authors":"Michael Bouaziz MD ,&nbsp;Jessica G. Lee MD","doi":"10.1016/j.oret.2024.09.004","DOIUrl":"10.1016/j.oret.2024.09.004","url":null,"abstract":"","PeriodicalId":19501,"journal":{"name":"Ophthalmology. Retina","volume":"9 5","pages":"Page e45"},"PeriodicalIF":4.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}