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Evaluation of nucleotide derivatives and sugar nucleotide analogs as inhibitors of glycosyltransferases. 核苷酸衍生物和糖核苷酸类似物作为糖基转移酶抑制剂的评价。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.89
K Osumi, N Kawauchi, A H Lu, O Hindsgaul, M M Palcic
{"title":"Evaluation of nucleotide derivatives and sugar nucleotide analogs as inhibitors of glycosyltransferases.","authors":"K Osumi,&nbsp;N Kawauchi,&nbsp;A H Lu,&nbsp;O Hindsgaul,&nbsp;M M Palcic","doi":"10.1093/nass/44.1.89","DOIUrl":"https://doi.org/10.1093/nass/44.1.89","url":null,"abstract":"<p><p>The inhibition of glycosyltransferases was studied using uridine monophosphate derivatives and uridine diphosphate sugar analogs. Modification in the nucleoside portion caused selective inhibition of glycosyltransferases.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"89-90"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.89","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Automated, solid-phase coupling of rhodamine dye acids to 5' amino oligonucleotides. 罗丹明染料酸与5′氨基寡核苷酸的自动固相偶联。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.257
R Vinayak, H Tang
{"title":"Automated, solid-phase coupling of rhodamine dye acids to 5' amino oligonucleotides.","authors":"R Vinayak,&nbsp;H Tang","doi":"10.1093/nass/44.1.257","DOIUrl":"https://doi.org/10.1093/nass/44.1.257","url":null,"abstract":"<p><p>A convenient method has been developed for directly labeling the 5' amino group of oligonucleotides on solid-support with rhodamine dyes.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"257-8"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.257","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of human telomerase by nucleotide analogues bearing a hydrophobic group. 带有疏水基团的核苷酸类似物对人类端粒酶的抑制作用。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.175
T Yamaguchi, M Kawarai, Y Takeshita, F Ishikawa, M Saneyoshi
{"title":"Inhibition of human telomerase by nucleotide analogues bearing a hydrophobic group.","authors":"T Yamaguchi,&nbsp;M Kawarai,&nbsp;Y Takeshita,&nbsp;F Ishikawa,&nbsp;M Saneyoshi","doi":"10.1093/nass/44.1.175","DOIUrl":"https://doi.org/10.1093/nass/44.1.175","url":null,"abstract":"<p><p>Telomerase, which synthesizes telomeric DNA in eukaryotic cells, is classified as a reverse transcriptase. To clarify the susceptibility of telomerase to nucleoside 5'-triphosphates bearing a hydrophobic group on the base moiety, we studied the inhibitory effects of 2',3'-dideoxy-5-styryluridine 5'-triphosphate analogues and 9-(beta-D-arabinofuranosyl)-2-(p-n-butylanilino)purine 5'-triphosphate analogues on telomerase activity using a quantitative 'stretch PCR' assay. 2',3'-Dideoxy-5-styryluridine 5'-triphosphate (StddUTP) showed more potent inhibition than 2',3'-dideoxythymidine 5'-triphosphate (ddTTP). On the other hand, 9-(beta-D-arabinofuranosyl)-2-(p-n-butylphenyl)guanine 5'-triphosphate (BuParaGTP) showed no inhibition, even though 9-(beta-D-arabinofuranosyl)guanine 5'-triphosphate (araGTP) is a potent inhibitor of telomerase. The influence on telomerase of hydrophobic substituents on the base moieties of nucleotides is described.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"175-6"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22517916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Homogenous simultaneous detection of HCV RNA and internal control by two-color fluorescence real-time monitoring of isothermal sequence amplification with INAF probes. 用INAF探针等温扩增双色荧光实时监测HCV RNA的同质同时检测和内控。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.191
J Saitoh, T Taya, R Horie, T Hayashi, T Ishiguro
{"title":"Homogenous simultaneous detection of HCV RNA and internal control by two-color fluorescence real-time monitoring of isothermal sequence amplification with INAF probes.","authors":"J Saitoh,&nbsp;T Taya,&nbsp;R Horie,&nbsp;T Hayashi,&nbsp;T Ishiguro","doi":"10.1093/nass/44.1.191","DOIUrl":"https://doi.org/10.1093/nass/44.1.191","url":null,"abstract":"<p><p>We demonstrated the homogenous simultaneous detection of HCV RNA and internal control by two-color fluorescence real-time monitoring of isothermal sequence amplification with INAF probes.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"191-2"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22517924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New method for scanning of single-nucleotide polymorphisms (SNPs): recognition of guanine-guanine mismatches by dimeric naphthyridine. 扫描单核苷酸多态性的新方法:用二聚体萘啶识别鸟嘌呤-鸟嘌呤错配。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.119
S Sando, K Nakatani, I Saito
{"title":"New method for scanning of single-nucleotide polymorphisms (SNPs): recognition of guanine-guanine mismatches by dimeric naphthyridine.","authors":"S Sando,&nbsp;K Nakatani,&nbsp;I Saito","doi":"10.1093/nass/44.1.119","DOIUrl":"https://doi.org/10.1093/nass/44.1.119","url":null,"abstract":"<p><p>Dimeric naphthyridine was designed and synthesized as a novel ligand that specifically binds to G-G mismatch, one of four SNP types. In the presence of dimeric naphthyridine, CD spectra of the G-G mismatch containing duplex noticeably changed, being accompanied by the induced CD at 300-350 nm, whereas no CD spectral change was observed for normal duplex. DNaseI footprinting titration indicated a selective binding of dimeric naphthyridine to the G-G mismatch with a dissociation constant of 53 nM.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"119-20"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Enzymatic properties of Escherichia coli and human 7,8-dihydro-8-oxoguanine DNA glycosylases. 大肠杆菌和人7,8-二氢-8-氧鸟嘌呤DNA糖基酶的酶学性质。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.11
K Asagoshi, T Yamada, H Terato, Y Ohyama, H Ide
{"title":"Enzymatic properties of Escherichia coli and human 7,8-dihydro-8-oxoguanine DNA glycosylases.","authors":"K Asagoshi,&nbsp;T Yamada,&nbsp;H Terato,&nbsp;Y Ohyama,&nbsp;H Ide","doi":"10.1093/nass/44.1.11","DOIUrl":"https://doi.org/10.1093/nass/44.1.11","url":null,"abstract":"<p><p>Oxidative damage to DNA generates aberrant guanine bases such as 2,6-diamino-4-hydroxy-formamido-pyrimidine (Fapy) and 7,8-dihydro-8-oxoguanine (8-oxoG). Although synthetic oligonucleotides containing a single 8-oxoG have been widely used to study enzymatic processing of this lesion, the synthesis of oligonucleotides containing Fapy as a unique lesion has not been achieved to date. In this study, an oligonucleotide containing a single 2,6-diamino-4-hydroxy-5-(N-methyl)formamido-pyrimidine (me-Fapy, a methylated derivative of Fapy) was prepared by a DNA polymerase reaction and the subsequent alkali treatment. The repair activity of Fpg and hOGG1 proteins were compared using oligonucleotide substrates containing me-Fapy and 8-oxoG.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"11-2"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Study on highly diastereoselective synthesis of (2'R)-2'-deoxy[2'-2H]guanosine. 高度非对映选择性合成(2' r)-2'-脱氧[2'-2H]鸟苷的研究。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.25
E Kawashima, Y S Terui, Y Ishido, K Yokozeki
{"title":"Study on highly diastereoselective synthesis of (2'R)-2'-deoxy[2'-2H]guanosine.","authors":"E Kawashima,&nbsp;Y S Terui,&nbsp;Y Ishido,&nbsp;K Yokozeki","doi":"10.1093/nass/44.1.25","DOIUrl":"https://doi.org/10.1093/nass/44.1.25","url":null,"abstract":"<p><p>To develop an efficient method for the synthesis of a highly diasteroselective (2'R)-2'-deoxy[2'-2H]guanosine (1), studies of organic chemical conversion from 2'-bromo-2'-deoxy-N2-Isobutyryl-3',5'-O-TIPDS-guanosine (2) to 1 and a biological transdeoxyribofuranosylation of (2'R > 98% de)-2'-deoxy[2'-2H]uridine (4) were carried out. As the results, a highly diastereoselective synthesis of 1 was achieved by a biological transdeoxyribofuranosylation between 2,6-diaminopurine and 4 by the use of Enterobacter aerogenes AJ-11125, followed by treatment with adenosine deaminase. The results will be described in detail.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"25-6"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.25","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Random insertion and deletion mutagenesis for construction of protein library containing nonnatural amino acids. 非天然氨基酸蛋白文库构建的随机插入和删除突变。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.69
H Murakami, T Hohsaka, M Sisido
{"title":"Random insertion and deletion mutagenesis for construction of protein library containing nonnatural amino acids.","authors":"H Murakami,&nbsp;T Hohsaka,&nbsp;M Sisido","doi":"10.1093/nass/44.1.69","DOIUrl":"https://doi.org/10.1093/nass/44.1.69","url":null,"abstract":"<p><p>A new method was developed for the generation of a library of mutant proteins that contained nonnatural amino acids. The method, \"random insertion and deletion (RID) mutagenesis\", is based on the deletion of an arbitrary number of bases at random positions and, at the same time, the insertion of an arbitrary sequence into the same position. By using this method, randomly selected three consecutive bases in the gene of green fluorescence protein (GFP) were replaced by a CGGT 4-base codon. When this DNA library was expressed in E. coli, about 80% of colonies lost the fluorescence. The non-fluorescent colonies were picked up and the genes were sequenced. Replacement of three consecutive bases by CGGT 4-base codon was found in two of the four mutated genes.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"69-70"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.69","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22517646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Novel synthesis of a tetra-acridinyl peptide as a new DNA polyintercalator. 新型DNA多插层化合物四吖啶基肽的新合成。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.133
H Ueyama, M Waki, M Takagi, S Takenaka
{"title":"Novel synthesis of a tetra-acridinyl peptide as a new DNA polyintercalator.","authors":"H Ueyama,&nbsp;M Waki,&nbsp;M Takagi,&nbsp;S Takenaka","doi":"10.1093/nass/44.1.133","DOIUrl":"https://doi.org/10.1093/nass/44.1.133","url":null,"abstract":"Tetra-acridinyl peptide 2 was synthesized from Fmoc-Lys(Boc)-OH and Fmoc-Lys(Acr)-OH (1) with the peptide synthesizer. The CD measurement suggested that 2 forms a special organized structure by itself in buffered solution. Peptide 2 binds to double stranded DNA with a very large affinity constant, which is 10(3)-times larger than that of quinacrine. Spectrophotometric and hydrodynamic studies suggested that all of the acridinyl parts of 2 contribute to the intercalating interaction for the DNA binding. Our finding in this experiment demonstrates that polyintercalators such as 2 can be assembled quickly by the automated synthesizer.","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"133-4"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
NMR solution structure of the L 9.1a region of Tetrahymena group I intron. 四膜虫I族内含子l9.1 a区的核磁共振溶液结构。
Nucleic acids symposium series Pub Date : 2000-01-01 DOI: 10.1093/nass/44.1.281
M Amano
{"title":"NMR solution structure of the L 9.1a region of Tetrahymena group I intron.","authors":"M Amano","doi":"10.1093/nass/44.1.281","DOIUrl":"https://doi.org/10.1093/nass/44.1.281","url":null,"abstract":"<p><p>The solution structure of 20 mer RNA contained of the loop 9.1a region of Tetrahymena group I intron was studied by NMR. This RNA oligomer has hairpin and duplex structures at high concentration (1 mM) of the sample even at low NaCl concentration (5 mM). In the hairpin structure, GC base pairs by the loop-loop interaction are formed. As study of NOESY measurements, and by the compared with the sequence, this loop region is presumed to interact with the loop 5c.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":" 44","pages":"281-2"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22518602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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