Novel synthesis of a tetra-acridinyl peptide as a new DNA polyintercalator.

H Ueyama, M Waki, M Takagi, S Takenaka
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引用次数: 7

Abstract

Tetra-acridinyl peptide 2 was synthesized from Fmoc-Lys(Boc)-OH and Fmoc-Lys(Acr)-OH (1) with the peptide synthesizer. The CD measurement suggested that 2 forms a special organized structure by itself in buffered solution. Peptide 2 binds to double stranded DNA with a very large affinity constant, which is 10(3)-times larger than that of quinacrine. Spectrophotometric and hydrodynamic studies suggested that all of the acridinyl parts of 2 contribute to the intercalating interaction for the DNA binding. Our finding in this experiment demonstrates that polyintercalators such as 2 can be assembled quickly by the automated synthesizer.
新型DNA多插层化合物四吖啶基肽的新合成。
以Fmoc-Lys(Boc)-OH和Fmoc-Lys(Acr)-OH(1)为原料,利用多肽合成器合成了四吖啶基肽2。CD测量表明,2在缓冲溶液中自行形成特殊的组织结构。肽2与双链DNA的结合具有非常大的亲和力常数,比醌大10(3)倍。分光光度和流体动力学研究表明,2的所有吖啶基部分都参与了DNA结合的插层相互作用。我们在这个实验中的发现表明,像2这样的多插层可以通过自动合成器快速组装。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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