New method for scanning of single-nucleotide polymorphisms (SNPs): recognition of guanine-guanine mismatches by dimeric naphthyridine.

S Sando, K Nakatani, I Saito
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引用次数: 1

Abstract

Dimeric naphthyridine was designed and synthesized as a novel ligand that specifically binds to G-G mismatch, one of four SNP types. In the presence of dimeric naphthyridine, CD spectra of the G-G mismatch containing duplex noticeably changed, being accompanied by the induced CD at 300-350 nm, whereas no CD spectral change was observed for normal duplex. DNaseI footprinting titration indicated a selective binding of dimeric naphthyridine to the G-G mismatch with a dissociation constant of 53 nM.

扫描单核苷酸多态性的新方法:用二聚体萘啶识别鸟嘌呤-鸟嘌呤错配。
二聚萘啶被设计和合成为一种新的配体,可以特异性结合四种SNP类型之一的G-G错配。在二聚萘啶的存在下,含G-G错配双相的CD光谱在300-350 nm处发生了明显的变化,而正常双相的CD光谱没有变化。dna足迹滴定表明二聚萘啶选择性结合G-G错配,解离常数为53 nM。
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