Odontology最新文献

{"title":"In vitro biocompatibility evaluation of three-dimensionally printed resin crowns for primary molars.","authors":"Ju Ri Ye, Kyu Hwan Kwack, Hyo-Jin An, Ji-Won Choi, Yong Kwon Chae, Mi Sun Kim, Hyeonjong Lee, Hyo-Seol Lee, Sung Chul Choi, Ok Hyung Nam","doi":"10.1007/s10266-025-01307-x","DOIUrl":"10.1007/s10266-025-01307-x","url":null,"abstract":"<p><p>This study aimed to assess the in vitro biocompatibility of two types of three-dimensionally (3D)-printed resin crowns (RCs) for primary molars. Three types of crowns were tested: (i) stainless steel crowns (SSCs), (ii) prefabricated zirconia crowns (PZCs), and (iii) 3D-printed RCs, which included two types: DIOnavi-P. MAX (RC1), and NextDent C&B Micro Filled Hybrid (RC2). Human gingival fibroblast (HGF) cells were exposed to extracts obtained from the crowns. Cell viability, proliferation, and migration were evaluated using the Cell Counting Kit-8 assay, flow cytometry, and a scratch wound healing assay. Oxidative stress levels were analyzed using a reactive oxygen species (ROS) assay. Expression of inflammatory biomarkers was assessed using a nitric oxide assay and reverse transcription quantitative polymerase chain reaction, and further confirmed by Western blotting. RC groups had a limited effect on HGF cell viability, proliferation, and migration. ROS levels were significantly lower in the RC groups than in the lipopolysaccharide group (p < 0.05). No significant upregulation of inflammatory biomarkers was observed in the RC groups compared to the other crown groups (p > 0.05). Within the limits of this study, 3D-printed RCs showed biocompatibility. These findings highlight the possibility of the clinical application of 3D-printed RCs for primary molars. Our results support that 3D-printed RCs for primary molars can offer an esthetic option with biocompatibility in gingival tissues, causing minimal inflammation.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2405-2414"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of contamination stage and adhesive strategy on the durability of repair bond strength of blood-saliva contaminated aged composite resin.","authors":"Cemile Yılmaz, İsmail Haktan Çelik","doi":"10.1007/s10266-026-01543-9","DOIUrl":"10.1007/s10266-026-01543-9","url":null,"abstract":"<p><p>This study evaluated whether the stage of contamination (before vs. after adhesive polymerization) and the application strategy of a universal adhesive (GLUMA Bond Universal, Kulzer, Hanau, Germany) used in total-etch (TE) or self-etch (SE) mode affect the durability of repair shear bond strength (RSBS) in aged composite after immediate storage (24 h) and artificial aging (10.000 thermocycles). One hundred twenty composite specimens were thermocycled (5.000 cycles) and assigned to 12 groups based on contamination stage (control, pre-adhesive, post-adhesive), adhesive strategy (TE or SE), and aging period (24 h or 10.000 cycles). A fresh blood/saliva mixture was applied for 1 min and rinsed. In post-adhesive groups, re-bonding was performed, with additional re-etching in the TE subgroup. RSBS was measured at a crosshead speed of 1 mm/min. Data were analyzed by three-way robust ANOVA based on 20% trimmed means (α = 0.05). Effect sizes were estimated using bootstrap partial eta squared (η²p) with 95% confidence intervals. Failure modes were analyzed using Fisher's Exact Test. All main effects were significant: contamination stage (η²p = 0.191, 95% CI: 0.093-0.309), adhesive strategy (η²p = 0.360, 95% CI: 0.263-0.478), and aging period (η²p = 0.805, 95% CI: 0.799-0.838) (all p ≤ 0.001). Significant interactions emerged between contamination stage and aging (p = 0.020, η²p = 0.070, 95% CI: 0.012-0.161) and between adhesive strategy and aging (p = 0.001, η²p = 0.133, 95% CI: 0.047-0.254). The three-way interaction was not significant (p = 0.078). Pre-adhesive contamination significantly reduced bond strength compared to the control and post-adhesive conditions (p < 0.001). TE showed higher RSBS than SE (p < 0.001), whereas aging reduced RSBS (p = 0.001). The highest RSBS was observed in the control + TE + 24 h group (31.11 ± 1.32 MPa), and the lowest in the pre-adhesive + SE + 10.000 TC group (5.33 ± 0.56 MPa). Failure mode distribution shifted from cohesive to adhesive after aging. Re-bonding after post-adhesive contamination restored bond strength to control levels. In conclusion, pre-adhesive blood/saliva contamination is most detrimental to repair durability; the total-etch protocol produced higher RSBS values than the self-etch protocol, although both strategies demonstrated significant degradation after thermal aging.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2775-2788"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tropomyosin-1 positively regulates TGF-β/Smad3 signaling through STUB1 in arecoline-induced oral submucosal fibrosis.","authors":"Rong Zhu, Lin Yin, Rong Zhou, Xin Zhang","doi":"10.1007/s10266-025-01244-9","DOIUrl":"10.1007/s10266-025-01244-9","url":null,"abstract":"<p><p>Oral submucous fibrosis (OSF) is a precancerous condition primarily caused by arecoline in betel nuts. The transforming growth factor (TGF)-β/mothers against decapentaplegic homolog 3 (Smad3) signaling pathway plays a pivotal role in its pathogenesis. This study explores the interaction between tropomyosin-1 (TPM1) and STIP1 homology and U-Box-containing protein 1 (STUB1) in regulating this pathway and its impact on OSF progression. We found that arecoline dose and time-dependently upregulates the expression of TPM1 mRNA and protein in human oral fibroblasts (HOrF). Treating HOrF with 20 μg/mL arecoline for 48 h could effectively drive fibroblast proliferation, fibrosis, migration and invasion. TPM1 knockdown reversed these effects. Mechanistically, arecoline upregulates TPM1 by activating the TGF-β/Smad3 signaling pathway. Notably, TPM1 overexpression rescued TGF-β/Smad3 activity even in the presence of the TGF-β inhibitor SB431542, revealing a positive feedback loop. Additionally, Western blotting and co-immunoprecipitation (Co-IP) analyses showed that arecoline downregulates STUB1, thereby inhibiting the ubiquitination of TGF-β/Smad3 in HOrF. Meanwhile, TPM1 competitively binds to STUB1, blocking its interaction with TGF-β/Smad3 and thereby stabilizing the pathway, which exacerbates fibrosis. Statistical analysis (one-way ANOVA with Tukey's HSD post-hoc test or independent-samples t-test) confirmed the significance of all major findings (p < 0.05). In conclusion, activation of the TGF-β/Smad3 pathway upregulates TPM1, which in turn blocks STUB1-mediated ubiquitination of the same cascade, establishing a positive-feedback loop that exacerbates arecoline-induced OSF. These findings improve our understanding of OSF's molecular pathogenesis and offer a potential target for its prevention and treatment.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1873-1886"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the wide applications of microfluidic devices in peri-implantitis, periodontitis, and endodontics.","authors":"Mitra Rostami, Fatemeh Ostadkhalil, Naghmeh Shenasa, Nawfal Yousif Jamil, Karar H Alfarttoosi, Sada Ghalib Taher, Mariem Alwan, Mahmood Jawad, Hiba Mushtaq, Ailar Yousefbeigi","doi":"10.1007/s10266-026-01311-9","DOIUrl":"10.1007/s10266-026-01311-9","url":null,"abstract":"<p><p>Periodontitis (PD) is a chronic inflammatory disease driven by a dysbiotic oral biofilm, leading to the progressive destruction of the tooth-supporting tissues. Peri-implantitis (PI) involves a similar plaque-induced inflammatory process that results in the loss of bone around dental implants. Endodontic diseases are primarily infections of the dental pulp and periapical tissues. The effective management of these conditions depends on accurate diagnosis and the removal of microbial biofilms from root canals, periodontal pockets, and implant surfaces. However, the development of advanced regenerative therapies has been hindered by a lack of preclinical models that can faithfully replicate the complex pathophysiology of human dental tissues. The integration of microfluidic devices (MFDs) offers a transformative approach for diagnostics and therapeutic screening. These platforms enable the precise analysis of microbial communities and host-derived biomarkers with high sensitivity. Concurrently, organ-on-a-chip (OOAC) technology provides a sophisticated in vitro methodology to model the dynamic, multi-tissue environments of the oral cavity, enabling real-time study of host-microbe interactions, disease progression, and treatment responses. This review examines the converging roles of MFD-based diagnostic tools and OOAC disease models in advancing the understanding and treatment of PD, PI, and endodontic infections. We highlight how these technologies address critical gaps in current dental research and discuss their potential to bridge diagnostic discovery with regenerative therapy development.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1801-1819"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146011370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects of different surface modification techniques on the optical properties of monolithic zirconia in simulated gastric acid and neutral media.","authors":"Kevser Karakaya, Özer İşisağ, Oğuzhan Evcin, Ayşe Aksu","doi":"10.1007/s10266-025-01249-4","DOIUrl":"10.1007/s10266-025-01249-4","url":null,"abstract":"<p><p>Monolithic zirconia has become increasingly popular in prosthodontics due to its favorable mechanical and esthetic properties. However, exposure to acidic conditions, such as simulated gastric acid, may compromise its optical and surface characteristics. This in vitro study aimed to evaluate the effects of three different surface modification technique mechanical polishing, polishing followed by glaze application, and polishing followed by zirconia-silica coating on the color stability and surface roughness of monolithic zirconia under acidic and neutral conditions. Forty-eight monolithic zirconia specimens were randomly assigned to six groups according to the surface modification technique and immersion medium (distilled water or simulated gastric acid). Color differences (ΔE₀₀) were calculated using the CIEDE2000 formula, and surface roughness was assessed using Atomic Force Microscopy (AFM). Data were analyzed using two-way ANOVA and Tukey's HSD post hoc test (α = 0.05). Surface modification technique significantly affected ΔE₀₀ values (p = 0.00015), whereas neither the immersion medium nor the interaction between the two factors was statistically significant. The mechanically polished group exhibited the highest color change. Regarding surface roughness, exposure to acidic conditions resulted in smoother surfaces in the mechanically polished and polished + glaze groups, whereas an increase in roughness was observed in the zirconia-silica-coated group. Surface modification techniques play a critical role in the esthetic and structural integrity of monolithic zirconia restorations. Glazing and zirconia-silica coating improved color stability and maintained surface characteristics more effectively than mechanical polishing alone. The influence of solution pH was minimal. These findings support the use of appropriate finishing techniques to enhance the clinical longevity of zirconia restorations.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1914-1924"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enamel remineralization potential of bioactive glass-containing toothpastes: an in vitro study.","authors":"Pakize Rana Çetin, Elif Alkan, Dilek Tağtekin, Ali İhsan Çetin, Nihal Şehkar Oktay","doi":"10.1007/s10266-026-01337-z","DOIUrl":"10.1007/s10266-026-01337-z","url":null,"abstract":"<p><p>This study evaluated the remineralization potential of experimental containing bioactive glass, hydroxyapatite, protein-calcium glycerophosphate, and their fluoride-enriched formulations on artificially demineralized enamel. Eighty extracted human molars were sectioned into enamel specimens, and artificial caries lesions were induced. Specimens were randomly divided into eight groups (n = 10): experimental formulations (bioactive glass (MB), bioactive glass + fluoride (MBF), hydroxyapatite (MH), hydroxyapatite + fluoride (MHF), protein + calcium glycerophosphate (MP)), commercial toothpastes (Sensodyne Repair & Protect with NovaMin (MS), R.O.C.S. Sensitive (MR)), and a control group. All specimens underwent a 14-day pH-cycling protocol simulating daily demineralization and remineralization. Remineralization efficacy was assessed at baseline, post-demineralization, and post-treatment using DIAGNOdent Pen, FluoreCam imaging, and Vickers microhardness testing. Data were analyzed using ANOVA. Significance level was set at p < 0.05. The highest microhardness recovery occurred in MHF (60.65 ± 21.92) and MS (57.07 ± 24.14), while the least increase was observed in the control (9.46 ± 14.28) and MP (15.5 ± 17.23) (p < 0.001). FluoreCam analysis revealed the greatest mineral gain in MHF (4.22 ± 2.24), MS (4.12 ± 1.57), and MBF (4.02 ± 2.73); however, no significance was detected between treatment groups (p = 0.419). DIAGNOdent Pen values were lowest post-treatment in MR (6.7 ± 0.9), MBF (6.9 ± 0.94), and MS (6.9 ± 1.14), while all treatment groups had significantly lower values than the control (11.1 ± 1.45) (p < 0.001). Experimental toothpastes containing bioactive glass, hydroxyapatite, and protein- calcium glycerophosphate, as well as commercial products containing NovaMin and hydroxyapatite, supported enamel remineralization. The addition of fluoride enhanced this effect. Clinical significance This study demonstrates the potential of various remineralization agents to strengthen early enamel lesions, offering clinicians alternative non-invasive options for early caries management.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2669-2682"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bleaching agents containing nano-hydroxyapatite on sound and demineralized enamel: an in vitro study.","authors":"Merve Haberal, Çiğdem Çelik","doi":"10.1007/s10266-026-01328-0","DOIUrl":"10.1007/s10266-026-01328-0","url":null,"abstract":"<p><p>This in vitro study aimed to evaluate the effects of office and home bleaching agents containing nano-hydroxyapatite (n-HAP) on the surface roughness, microhardness, and micromorphology of sound and demineralized enamel. A total of 120 enamel specimens were obtained from extracted human third molars, half of which underwent a demineralization cycle to simulate initial enamel demineralization. The specimens were divided into five subgroups (n = 12) based on the bleaching agent applied: no bleaching (control), 40% hydrogen peroxide (HP), 16% carbamide peroxide (CP), 40% HP + n-HAP, and 6% HP + n-HAP. Surface roughness was measured using non-contact optical profilometry, and microhardness was assessed via the Vickers hardness test. Micromorphological changes were examined by scanning electron microscopy (SEM) at × 1,000 and × 4,000 magnifications. Statistical analyses were performed using the Shapiro-Wilk, Mann-Whitney U, Kruskal-Wallis H, and Bonferroni post hoc tests (p < 0.05). In the demineralized groups, surface roughness significantly increased while microhardness decreased (p < 0.05). The highest surface roughness was observed in the demineralized group treated with 16% CP, whereas microhardness significantly increased in the group treated with 6% HP + n-HAP. SEM findings revealed pronounced structural degradation in groups treated with high-concentration HP, while n-HAP-containing groups exhibited mineral deposition and preservation of the prismatic enamel structure. Although no remineralization protocol was used before bleaching, the findings indicate that low-concentration HP agents containing n-HAP may protect enamel with initial demineralization. These results highlight the potential clinical benefit of such formulations in reducing bleaching-related enamel damage.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2563-2579"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of addition of hydroxyapatite nanofibers to glass ionomer cement on fracture toughness, microhardness, and fluoride release.","authors":"Mai Samy Sheta, El-Refaie Kenawy, Samar A Khattab","doi":"10.1007/s10266-026-01308-4","DOIUrl":"10.1007/s10266-026-01308-4","url":null,"abstract":"<p><p>The current study attempted to investigate the effect of hydroxyapatite nanofibers (HANFs) added in different percentages upon the qualities of glass ionomer cement (GIC): fracture toughness, vickers microhardness and fluoride release. Synthesis and characterization of hydroxyapatite nanofibers were carried out before being added to the powder of high-viscosity conventional GIC in three varied weight ratios (1, 3 and 5%) resulting into 3 experimental groups which were next compared with basic GIC as a control group. Fracture toughness, Vickers microhardness and fluoride release on day1 and day 7 were evaluated. Results indicated significant improvement in fracture toughness, microhardness and fluoride release of GIC modified by hydroxyapatite nanofibers at varied tested ratios and this increase was directly proportional to their ratio of addition. According to these findings, glass ionomer cement modified with hydroxyapatite nanofibers in varying proportions could be a dependable restorative material with enhanced fracture toughness, microhardness and fluoride release.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2445-2454"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical performance of BioCoat™ and Fissurit FX: a randomized 18-month split-mouth study.","authors":"Esra Ergün, Canan Bayraktar Nahir","doi":"10.1007/s10266-026-01332-4","DOIUrl":"10.1007/s10266-026-01332-4","url":null,"abstract":"<p><p>Pit and fissure sealants (PFS) are effective in preventing occlusal caries. This randomized split-mouth clinical study aimed to compare the 18-month clinical performance of the bioactive resin-based PFS (BioCoat™) with a fluoride-containing conventional resin-based PFS (Fissurit FX) and to assess the influence of patient-related factors on material success. Sixty-three pediatric patients (aged 7-9) requiring non-invasive PFS were included. BioCoat™ (Group 1) and Fissurit FX (Group 2) were applied to contralateral mandibular permanent first molars. Clinical evaluations were performed at 3rd, 6th, 9th, 12th, and 18th months using modified USPHS criteria. Kaplan-Meier survival analysis and logistic regression were applied. At 18 months, success rates were 90.2% for BioCoat™ and 88.2% for Fissurit FX, with no statistically significant difference between groups (p > 0.05). Both materials showed similar performance in marginal discoloration-adaptation, retention and secondary caries (p > 0.05). Median survival times were 17.72 months (BioCoat™) and 17.49 months (Fissurit FX), showing no significant difference (p = 0.744). No significant effect of patient-related factors on material success was determined (p > 0.05). BioCoat™ demonstrated comparable long-term clinical performance to Fissurit FX. Its bioactive properties may provide additional preventive benefits, supporting its safe use in pediatric dentistry.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2637-2647"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146106519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of vitamins A/C primed human gingival mesenchymal stem/progenitor cells in preventing medication-related osteonecrosis of extraction socket: an animal study.","authors":"Fatma I Elfaiedi, Nahed S Korany, Karim Fawzy El-Sayed, Nermeen AbuBakr","doi":"10.1007/s10266-025-01288-x","DOIUrl":"10.1007/s10266-025-01288-x","url":null,"abstract":"<p><p>Human gingival mesenchymal stem/progenitor cells (hGMSCs) exert remarkable tissue regeneration properties. A combination of vitamins C and A at specified concentrations enhanced cellular dedifferentiation and multipotency of hGMSCs. To our knowledge, vitamins A/C-primed hGMSCs in the prevention of medication-related osteonecrosis of the jaw (MRONJ) have not been investigated. Thus, this study evaluated the impact of vitamins A/C in pluripotency-inductive concentrations on the regenerative/reparative potential of hGMSCs in preventing MRONJ development in rats. Thirty albino rats (n = 10/group) were administered zoledronic acid and dexamethasone three times a week for 4 weeks. The upper first molar was extracted 2 weeks following the first administration. Immediately after extraction, rats received normal saline (group Ι), hGMSCs (group ΙΙ), and hGMSCs primed with vitamins A/C (group ΙΙΙ). Rats were euthanized 2 and 4 weeks after extraction. The maxillae were analyzed using dual-energy X-ray absorptiometry, histologically, histomorphometrically, and by quantitative real-time polymerase chain reaction analysis. Groups II and III displayed elevated bone mineral density, with a significant rise in group ΙΙΙ relative to group ΙΙ at 4 weeks. Histomorphometrically, groups II and III revealed an elevation in area percentage of newly generated bone, with a significant rise in group ΙΙΙ at both time intervals. Gene expression of transforming growth factor-β1 (TGF-β1) and runt-related transcription factor 2 (Runx2) presented a significant elevation in groups II and III, with a significant rise in group ΙΙΙ at both time intervals. In conclusion, vitamins A/C-primed hGMSCs at the tested pluripotency-inductive concentrations prevented MRONJ development, primarily through encouraging wound healing and bone repair in the extraction sockets of rats.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2214-2225"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145896645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}