Odontology最新文献

{"title":"Illuminant-dependent color inconstancy and metameric behavior of anodized titanium through silicone-based gingival simulation layers of varying thickness.","authors":"Akanay Copuroglu, Umur Davut, Ozge Doganay Ozyilmaz, Ozgun Yusuf Ozyilmaz","doi":"10.1007/s10266-026-01564-4","DOIUrl":"https://doi.org/10.1007/s10266-026-01564-4","url":null,"abstract":"<p><p>This in vitro study evaluated how gingival thickness and anodization color affect color perception of Grade V titanium discs under different light sources. Ninety-six titanium discs were anodized yellow, pink, or purple, or left non-anodized. Silicone gingival masks of 1 mm and 2 mm simulated soft tissue. A single spectral reflectance measurement was recorded for each specimen using a benchtop spectrophotometer, from which color coordinates under five standardized illuminants (D65, D50, A, F2, F11) were computationally derived. Color differences (ΔE₀₀) were calculated using the CIEDE2000 formula to evaluate color inconstancy, and metamerism was quantified using a CIEDE2000-based metamerism index (MI), the mean absolute difference between ΔE₀₀ under the test illuminants (D50, A, F2, F11) and D65. Data were analyzed with one-way ANOVA, Tukey HSD post hoc tests, and independent t-tests (α = 0.05). All anodized colors at 1 mm exceeded the CIEDE2000 clinical acceptability threshold (ΔE₀₀ >1.8), with purple showing the highest inconstancy, particularly under fluorescent lighting (P<.05). Increasing thickness to 2 mm significantly reduced ΔE₀₀ across all groups (P<.05), although values remained above the acceptability threshold in every group, with purple showing the greatest relative stability under all illuminants. Metameric behavior remained imperceptible under most conditions, except for pink at 1 mm under F2. Thickness and lighting significantly affect color stability of anodized titanium, although the effect of illuminant type was color-dependent: fluorescent (F2) illumination increased color inconstancy for purple and, at 2 mm, yellow, whereas pink showed the opposite pattern, with its lowest ΔE₀₀ values occurring under F2 rather than D65 at both thicknesses. Thinner tissue intensifies color shifts, while ≥ 2 mm thickness improves consistency. Pink performs better in thin tissue; purple shows greatest stability with thicker coverage. Metamerism was imperceptible under most conditions, except for pink at 1 mm under F2, which reached a perceptible but clinically acceptable level.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149112615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KeratoDynamics of transdifferentiation and dyskeratosis in odontogenic keratocysts.","authors":"Krishnasamy Nitya, Ramadoss Ramya, Suresh Vemuri","doi":"10.1007/s10266-025-01247-6","DOIUrl":"10.1007/s10266-025-01247-6","url":null,"abstract":"<p><p>Odontogenic keratocysts (OKCs) exhibit paradoxical biological behavior, displaying both cystic architecture and neoplastic potential. Their recurrence and local aggressiveness are believed to stem from epithelial plasticity and aberrant keratinization. This study introduces the concept of KeratoDynamics, a molecular framework integrating the processes of transdifferentiation and dyskeratosis in OKCs, through the analysis of three key biomarkers-Ki-67, c-Myc, and CK19. Formalin-fixed, paraffin-embedded samples of recurrent and non-recurrent OKCs were examined histologically and immunohistochemically. Quantitative assessment revealed significantly higher Ki-67 (42%) and c-Myc (38%) expression in recurrent lesions compared with non-recurrent cases (25% and 20%, respectively; p < 0.05). CK19 exhibited diffuse cytoplasmic positivity, with greater intensity in recurrent OKCs, indicating disrupted epithelial differentiation. A strong correlation between Ki-67 and c-Myc (r = 0.68, p < 0.01) reflected a proliferative-transcriptional coupling, while CK19 expression correlated moderately with delayed recurrence (r = 0.52, p < 0.05). Histologically, recurrent lesions demonstrated epithelial budding, dyskeratosis, and focal thickening, consistent with molecular reprogramming and epithelial instability. The collective expression of these markers defines the KeratoDynamics panel, which encapsulates the continuum between proliferation, lineage reprogramming, and keratinization that underlies OKC pathogenesis. This integrative approach enhances diagnostic precision, aids in recurrence prediction, and offers new perspectives for biologically guided management of odontogenic keratocysts.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1897-1902"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro biocompatibility evaluation of three-dimensionally printed resin crowns for primary molars.","authors":"Ju Ri Ye, Kyu Hwan Kwack, Hyo-Jin An, Ji-Won Choi, Yong Kwon Chae, Mi Sun Kim, Hyeonjong Lee, Hyo-Seol Lee, Sung Chul Choi, Ok Hyung Nam","doi":"10.1007/s10266-025-01307-x","DOIUrl":"10.1007/s10266-025-01307-x","url":null,"abstract":"<p><p>This study aimed to assess the in vitro biocompatibility of two types of three-dimensionally (3D)-printed resin crowns (RCs) for primary molars. Three types of crowns were tested: (i) stainless steel crowns (SSCs), (ii) prefabricated zirconia crowns (PZCs), and (iii) 3D-printed RCs, which included two types: DIOnavi-P. MAX (RC1), and NextDent C&B Micro Filled Hybrid (RC2). Human gingival fibroblast (HGF) cells were exposed to extracts obtained from the crowns. Cell viability, proliferation, and migration were evaluated using the Cell Counting Kit-8 assay, flow cytometry, and a scratch wound healing assay. Oxidative stress levels were analyzed using a reactive oxygen species (ROS) assay. Expression of inflammatory biomarkers was assessed using a nitric oxide assay and reverse transcription quantitative polymerase chain reaction, and further confirmed by Western blotting. RC groups had a limited effect on HGF cell viability, proliferation, and migration. ROS levels were significantly lower in the RC groups than in the lipopolysaccharide group (p < 0.05). No significant upregulation of inflammatory biomarkers was observed in the RC groups compared to the other crown groups (p > 0.05). Within the limits of this study, 3D-printed RCs showed biocompatibility. These findings highlight the possibility of the clinical application of 3D-printed RCs for primary molars. Our results support that 3D-printed RCs for primary molars can offer an esthetic option with biocompatibility in gingival tissues, causing minimal inflammation.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2405-2414"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of contamination stage and adhesive strategy on the durability of repair bond strength of blood-saliva contaminated aged composite resin.","authors":"Cemile Yılmaz, İsmail Haktan Çelik","doi":"10.1007/s10266-026-01543-9","DOIUrl":"10.1007/s10266-026-01543-9","url":null,"abstract":"<p><p>This study evaluated whether the stage of contamination (before vs. after adhesive polymerization) and the application strategy of a universal adhesive (GLUMA Bond Universal, Kulzer, Hanau, Germany) used in total-etch (TE) or self-etch (SE) mode affect the durability of repair shear bond strength (RSBS) in aged composite after immediate storage (24 h) and artificial aging (10.000 thermocycles). One hundred twenty composite specimens were thermocycled (5.000 cycles) and assigned to 12 groups based on contamination stage (control, pre-adhesive, post-adhesive), adhesive strategy (TE or SE), and aging period (24 h or 10.000 cycles). A fresh blood/saliva mixture was applied for 1 min and rinsed. In post-adhesive groups, re-bonding was performed, with additional re-etching in the TE subgroup. RSBS was measured at a crosshead speed of 1 mm/min. Data were analyzed by three-way robust ANOVA based on 20% trimmed means (α = 0.05). Effect sizes were estimated using bootstrap partial eta squared (η²p) with 95% confidence intervals. Failure modes were analyzed using Fisher's Exact Test. All main effects were significant: contamination stage (η²p = 0.191, 95% CI: 0.093-0.309), adhesive strategy (η²p = 0.360, 95% CI: 0.263-0.478), and aging period (η²p = 0.805, 95% CI: 0.799-0.838) (all p ≤ 0.001). Significant interactions emerged between contamination stage and aging (p = 0.020, η²p = 0.070, 95% CI: 0.012-0.161) and between adhesive strategy and aging (p = 0.001, η²p = 0.133, 95% CI: 0.047-0.254). The three-way interaction was not significant (p = 0.078). Pre-adhesive contamination significantly reduced bond strength compared to the control and post-adhesive conditions (p < 0.001). TE showed higher RSBS than SE (p < 0.001), whereas aging reduced RSBS (p = 0.001). The highest RSBS was observed in the control + TE + 24 h group (31.11 ± 1.32 MPa), and the lowest in the pre-adhesive + SE + 10.000 TC group (5.33 ± 0.56 MPa). Failure mode distribution shifted from cohesive to adhesive after aging. Re-bonding after post-adhesive contamination restored bond strength to control levels. In conclusion, pre-adhesive blood/saliva contamination is most detrimental to repair durability; the total-etch protocol produced higher RSBS values than the self-etch protocol, although both strategies demonstrated significant degradation after thermal aging.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2775-2788"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tropomyosin-1 positively regulates TGF-β/Smad3 signaling through STUB1 in arecoline-induced oral submucosal fibrosis.","authors":"Rong Zhu, Lin Yin, Rong Zhou, Xin Zhang","doi":"10.1007/s10266-025-01244-9","DOIUrl":"10.1007/s10266-025-01244-9","url":null,"abstract":"<p><p>Oral submucous fibrosis (OSF) is a precancerous condition primarily caused by arecoline in betel nuts. The transforming growth factor (TGF)-β/mothers against decapentaplegic homolog 3 (Smad3) signaling pathway plays a pivotal role in its pathogenesis. This study explores the interaction between tropomyosin-1 (TPM1) and STIP1 homology and U-Box-containing protein 1 (STUB1) in regulating this pathway and its impact on OSF progression. We found that arecoline dose and time-dependently upregulates the expression of TPM1 mRNA and protein in human oral fibroblasts (HOrF). Treating HOrF with 20 μg/mL arecoline for 48 h could effectively drive fibroblast proliferation, fibrosis, migration and invasion. TPM1 knockdown reversed these effects. Mechanistically, arecoline upregulates TPM1 by activating the TGF-β/Smad3 signaling pathway. Notably, TPM1 overexpression rescued TGF-β/Smad3 activity even in the presence of the TGF-β inhibitor SB431542, revealing a positive feedback loop. Additionally, Western blotting and co-immunoprecipitation (Co-IP) analyses showed that arecoline downregulates STUB1, thereby inhibiting the ubiquitination of TGF-β/Smad3 in HOrF. Meanwhile, TPM1 competitively binds to STUB1, blocking its interaction with TGF-β/Smad3 and thereby stabilizing the pathway, which exacerbates fibrosis. Statistical analysis (one-way ANOVA with Tukey's HSD post-hoc test or independent-samples t-test) confirmed the significance of all major findings (p < 0.05). In conclusion, activation of the TGF-β/Smad3 pathway upregulates TPM1, which in turn blocks STUB1-mediated ubiquitination of the same cascade, establishing a positive-feedback loop that exacerbates arecoline-induced OSF. These findings improve our understanding of OSF's molecular pathogenesis and offer a potential target for its prevention and treatment.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1873-1886"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the wide applications of microfluidic devices in peri-implantitis, periodontitis, and endodontics.","authors":"Mitra Rostami, Fatemeh Ostadkhalil, Naghmeh Shenasa, Nawfal Yousif Jamil, Karar H Alfarttoosi, Sada Ghalib Taher, Mariem Alwan, Mahmood Jawad, Hiba Mushtaq, Ailar Yousefbeigi","doi":"10.1007/s10266-026-01311-9","DOIUrl":"10.1007/s10266-026-01311-9","url":null,"abstract":"<p><p>Periodontitis (PD) is a chronic inflammatory disease driven by a dysbiotic oral biofilm, leading to the progressive destruction of the tooth-supporting tissues. Peri-implantitis (PI) involves a similar plaque-induced inflammatory process that results in the loss of bone around dental implants. Endodontic diseases are primarily infections of the dental pulp and periapical tissues. The effective management of these conditions depends on accurate diagnosis and the removal of microbial biofilms from root canals, periodontal pockets, and implant surfaces. However, the development of advanced regenerative therapies has been hindered by a lack of preclinical models that can faithfully replicate the complex pathophysiology of human dental tissues. The integration of microfluidic devices (MFDs) offers a transformative approach for diagnostics and therapeutic screening. These platforms enable the precise analysis of microbial communities and host-derived biomarkers with high sensitivity. Concurrently, organ-on-a-chip (OOAC) technology provides a sophisticated in vitro methodology to model the dynamic, multi-tissue environments of the oral cavity, enabling real-time study of host-microbe interactions, disease progression, and treatment responses. This review examines the converging roles of MFD-based diagnostic tools and OOAC disease models in advancing the understanding and treatment of PD, PI, and endodontic infections. We highlight how these technologies address critical gaps in current dental research and discuss their potential to bridge diagnostic discovery with regenerative therapy development.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1801-1819"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146011370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects of different surface modification techniques on the optical properties of monolithic zirconia in simulated gastric acid and neutral media.","authors":"Kevser Karakaya, Özer İşisağ, Oğuzhan Evcin, Ayşe Aksu","doi":"10.1007/s10266-025-01249-4","DOIUrl":"10.1007/s10266-025-01249-4","url":null,"abstract":"<p><p>Monolithic zirconia has become increasingly popular in prosthodontics due to its favorable mechanical and esthetic properties. However, exposure to acidic conditions, such as simulated gastric acid, may compromise its optical and surface characteristics. This in vitro study aimed to evaluate the effects of three different surface modification technique mechanical polishing, polishing followed by glaze application, and polishing followed by zirconia-silica coating on the color stability and surface roughness of monolithic zirconia under acidic and neutral conditions. Forty-eight monolithic zirconia specimens were randomly assigned to six groups according to the surface modification technique and immersion medium (distilled water or simulated gastric acid). Color differences (ΔE₀₀) were calculated using the CIEDE2000 formula, and surface roughness was assessed using Atomic Force Microscopy (AFM). Data were analyzed using two-way ANOVA and Tukey's HSD post hoc test (α = 0.05). Surface modification technique significantly affected ΔE₀₀ values (p = 0.00015), whereas neither the immersion medium nor the interaction between the two factors was statistically significant. The mechanically polished group exhibited the highest color change. Regarding surface roughness, exposure to acidic conditions resulted in smoother surfaces in the mechanically polished and polished + glaze groups, whereas an increase in roughness was observed in the zirconia-silica-coated group. Surface modification techniques play a critical role in the esthetic and structural integrity of monolithic zirconia restorations. Glazing and zirconia-silica coating improved color stability and maintained surface characteristics more effectively than mechanical polishing alone. The influence of solution pH was minimal. These findings support the use of appropriate finishing techniques to enhance the clinical longevity of zirconia restorations.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1914-1924"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of individual sulfasalazine administration and in combination with esomeprazole on buccal mucosa of albino rats: histological, immunohistochemical, and molecular study.","authors":"Mona Bahaa Morsy, Marwa M Abd Elhameed, Reham Ahmed Mohamed Abdrabo, Safaa Ismail Hussein","doi":"10.1007/s10266-026-01350-2","DOIUrl":"10.1007/s10266-026-01350-2","url":null,"abstract":"<p><p>The study aims to investigate the effect of sulfasalazine (SFZN) individually and in combination with esomeprazole (ESOm) on the buccal mucosa of albino rats. SFZN is a widely used drug for the management of various autoimmune diseases which has been reported to cause renal injury in humans and a dose of 600 mg/kg/day has been described to cause renal injury in rats. ESOm is a commonly used proton pump inhibitor. Few studies have investigated their effects on oral and paraoral tissues. Three groups were designed out of 27 male albino rats. The control group was given distilled water, the SFZN group was given SFZN (600 mg/kg/day), and the ESOm group was given SFZN (600 mg/kg/day) and ESOm (30 mg/kg body weight); the drugs were dissolved in distilled water. The experiment was conducted for 14 days. Buccal mucosae were evaluated for keratin thickness, area % of iron deposition, area% of immunoreactivity to nuclear factor erythroid 2-related factor 2 (Nrf2) and glutathione (GSH) tissue level using one-way ANOVA and post hoc tests. The level of statistical significance was set at p < 0.05. SFZN individually has significantly increased keratin thickness, disrupted Nrf2 machinery and glutathione tissue level, while SFZN in combination with ESOm showed significant increase in area% of iron deposition. DNA degradation using comet assay was evaluated using Kruskal-Wallis and Dunn's test which revealed no significant difference of tail length and tail moment in SFZN group and ESOm group but these parameters were significantly different in these two groups in relation to the control group (p < 0.05).</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2693-2703"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147494478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative numerical finite element analysis of tooth-implant- versus implant-implant-supported three-unit fixed prostheses under oblique loading.","authors":"Hussain D Alsayed, Islam G Shahin, Noha M Anany, Mohamed I El-Anwar, Tarek M Elshazly, Abdulaziz Alhotan","doi":"10.1007/s10266-026-01336-0","DOIUrl":"10.1007/s10266-026-01336-0","url":null,"abstract":"<p><p>To investigate the biomechanical performance of fixed dental prostheses supported by either tooth-implant or implant-implant configurations, fabricated from zirconia or polyetherketoneketone (PEKK), under oblique loading using three-dimensional finite element analysis (FEA). Three mandibular posterior models were developed: M1 (implant at the first molar and natural tooth at the first premolar), M2 (implant at the first premolar and natural tooth at the first molar), and M3 (implants at both the first premolar and first molar). Each model was restored with a three-unit prosthesis fabricated from either zirconia or PEKK. The digital workflow included anatomical segmentation using Mimics software, refinement of bone and soft tissues with 3-Matic software, and integration of implants, abutments, and prosthetic components, followed by meshing and FEA using ANSYS software. All models were subjected to oblique loading of 50 N at 30° and 45°, and von Mises stress and deformation were recorded. M3 exhibited the most favorable biomechanical behavior, showing the lowest von Mises stress across almost all components. The hybrid tooth-implant models (M1 and M2) showed higher stress concentrations, particularly M2 with PEKK (loading angle 45°), which recorded the highest implant stress (312 MPa) and the greatest deformation (75 µm). The highest screw stress was recorded in M1 with PEKK, reaching 483 MPa. Zirconia exhibited higher stress within the prosthesis, but lower deformation, whereas PEKK resulted in greater deformation in the tooth and periodontal ligament (PDL). Implant-implant-supported prostheses showed the most favorable biomechanical behavior under oblique loading. Tooth-implant models, especially with anterior implant placement, exhibited higher stress and deformation. Zirconia offered greater rigidity and dimensional stability, while PEKK provided better stress dissipation but required careful design to manage its flexibility.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2648-2657"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of a 45S5 bioactive glass-modified self-etch adhesive on sound and artificially demineralized dentin: mechanical, interfacial, and chemical analyses.","authors":"Ana Laura Mendes Mota, Marcelo Victor Sidou Lemos, Mateus Soares de Araújo, Victoria Patrício de Oliveira Costa, Juliano Sartori Mendonça, Sérgio Lima Santiago","doi":"10.1007/s10266-026-01326-2","DOIUrl":"10.1007/s10266-026-01326-2","url":null,"abstract":"<p><p>To evaluate the effects on the bonding interface and remineralization potential of a self-etch adhesive containing 10% (w/v) 45S5 bioactive glass (BAG) in sound dentin (SD) and artificially demineralized dentin (ADD). Sound third molars were selected and stored in 0.01% thymol solution, and part of them was subjected to a pH-cycling protocol to induce controlled demineralization. The experimental groups were: SD, ADD, BAG + SD, and BAG + ADD. Microtensile bond strength (n = 8) was assessed after 24 h and six months. Nanoleakage (n = 3), micropermeability (n = 3), Knoop microhardness (n = 3 after 24 h and three months), and FT-IR spectroscopy (n = 3 immediately and after 30 days) were also performed. All specimens were stored in artificial saliva at 37 °C with monthly renewal. Data were analyzed using ANOVA and Tukey's test (p < 0.05). The BAG + ADD group maintained bond strength after 6 months (p = 0.887). BAG reduced nanoleakage in both SD and ADD. Micropermeability showed no fluorescein uptake in SD, and reduced absorption in ADD when treated with BAG. After 3 months, BAG + ADD significantly increased microhardness at all tested depths, while BAG + SD showed improvements at 70 µm and 100 µm (p = 0.006 and p = 0.015, respectively) FT-IR analysis showed phosphate and carbonate bands in BAG-containing adhesives, with increased definition after 30 days, indicating time-dependent ion release and apatite maturation. Incorporation of BAG into a self-etch adhesive (Clearfil SE Bond) preserved long-term bond strength, enhanced dentin microhardness, promoted mineral precipitation at the adhesive interface, and improved hybrid layer, sealing-particularly in artificially demineralized dentin.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2591-2603"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}