Novelty in Biomedicine最新文献

{"title":"Design and production of Arginine Deiminase-Azurin Recombinant Fusion Protein from Pseudomonas aeruginosa and its confirmation by Western blot","authors":"M. Hadi, M. Ghazi, P. Saffarian, Bahareh Hajikhani","doi":"10.22037/NBM.V1I1.26461","DOIUrl":"https://doi.org/10.22037/NBM.V1I1.26461","url":null,"abstract":"Background: Pseudomonas aeruginosa is a common Gram-negative, rod-shaped bacterium that has a unique genome that allowed the bacteria to produce various enzymes and proteins. Azurin and arginine deiminase are low molecular weight proteins that produced by P. aeruginosa. These proteins can be used alone or in combination together in order to become effective in cancer therapy or inhibiting of metastasis. This study aimed to design, express and purify the Azurin and Arginine Deiminease recombinant fusion protein. Materials and Methods: The sequences of Azurin and arginine deiminase from P. aeruginosa were studied for synthesis in a pET28a vector. The recombinant plasmid was transfected into the E. coli BL-21 strain and expression was induced by isopropyl-β-D-thio galactopyranoside (IPTG). The fusion protein expression was evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Purification of the recombinant product was performed by the Ni-NTA chromatography column, obtained product analysis with SDS-PAGE and Western blot technique. Results: Cloning was confirmed by observing bands from the enzyme digestion. The protein band with a molecular weight of 65 kDa on the SDS-PAGE gel was an indication of the correct expression of the protein. The single-band of this recombinant protein was confirmed by the western blot technique. Conclusion: In this study, due to the successful production of arginine deiminase-azurin fusion protein, and considering the separate anti-cancer properties of these compounds, which have been reported in previous studies, it is suggested that immunological assessments and effects of this fusion protein in different cancerous cell line be investigated.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"173 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73196686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a Comprehensive Evaluation Model for Health System Reform Plan in Iran: An Approach to Extended Balanced Scorecard","authors":"S. Janbazi, Mohammad Reza Rabiee Mandejin, Alireza Eslambulchi, A. Monfared","doi":"10.22037/NBM.V7I4.27831","DOIUrl":"https://doi.org/10.22037/NBM.V7I4.27831","url":null,"abstract":"Background: Health and striving to maintain and promote it has always been an important priority in governments, communities, and public policies. To implement health objectives through the Fifth Five-Year National Development Plan of Iran, the health system reform plan with three approaches to the financial protection of the public, creating equity in access to health services, and improving the quality of services has been on the government's agenda since 05.05.2014. Investigations revealed that the lack of a comprehensive and scientific model for evaluating this national program is evident. This study aimed to design a comprehensive model of health reform plan evaluation in Iran. What distinguishes this study from previous studies is the simultaneous attention to the challenges of performance evaluation, social responsibility, and examining them in a comprehensive and coherent model. M aterials and Methods: The present study considered as descriptive-survey research in terms of strategy. The research method was a combination that conducted from 2018 to 2019. The sample size of the qualitative part of the research consisted of 17 academic and executive health experts selected by purposive non-random sampling. In the quantitative part of the study, 400 health service providers selected by stratified random sampling. In this study, content analysis and Delphi technique, and Expert choice v24, SPSS V22 and AMOSV14 software were used to collect, classify, deduce and exploit data, and questionnaires. R es ults: The findings of this study resulted in the design of a 900-scorecard model of the comprehensive evaluation of the health system reform plan in Iran based on 5 dimensions (finance, social responsibility, growth and learning, clients and internal processes), 17 components and 70 indicators. C onclusion: The findings of the quantitative section of the study showed that the indicators, dimensions and levels of the comprehensive evaluation model for health system reform plan in Iran (CEHSRP-IR) qualified for evaluation of the effectiveness of this national program at different organizational levels and executing units of health system reform plan in Iran.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"2 1","pages":"187-200"},"PeriodicalIF":0.0,"publicationDate":"2019-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87727665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cell injection in two patients suffer from chronic discogenic low back pain","authors":"M. Hashemi, S. Moradi, M. Taheri, Siroos Momenzadeh, Alireza Shoae-Hassani, A. Ghamari, Hasti Hassani, M. Ghasemi, A. Solhpour, Payman Dadkhah","doi":"10.22037/NBM.V7I4.24886","DOIUrl":"https://doi.org/10.22037/NBM.V7I4.24886","url":null,"abstract":"Background: Discogenic low back pain is one of the leading causes of pain and disability across the world. A growing interest in the area of regenerative medicine, led by an improved understanding of the role of mesenchymal stem cells in tissue homeostasis and repair. Cases Report: We had two patients suffered from chronic discogenic low back pain. They were underwent injection of intra-discal 1.5 cc of Adipose Derived Mesenchymal Stem Cells (ADMSC) and followed up for 6 months. After this period of time, there was a significant reduction in both VAS and ODI scores in patients. Conclusion: These data warrant further studies so that we can enhance our understanding of the other unknown mechanisms, which may exist behind stem cell injection. If the effectiveness of such injection to reduce pain and improve function is shown in the upcoming studies , it may provide a new insight for increasing this method of treatment as a proper option in the near future. Cell Injection in Two Patients Suffer from Chronic Discogenic Low Back Pain. Novel Biomed. 2020; 8(2):95-7.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78686646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Assessment of Acupuncture and Exercise Therapy in Patients with Carpal Tunnel Syndrome: Randomized Clinical Trial","authors":"Shahin Salehi, Omid Hessami, Amir Rashed, M. Hassabi, Mehrshad PourSaeid Esfehani, Amirhosein Abedi Yakta, Hamid Mahdavi Mohtasham, S. Khosravi, Shahram Mohaghegh, M. Sohrabi","doi":"10.22037/NBM.V7I3.25274","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.25274","url":null,"abstract":"Background: The efficacy of acupuncture and exercise therapy in the treatment of carpal tunnel syndrome (CTS) has been investigated in limited studies with controversial results. The purpose of this study was to compare the short-term efficacy of acupuncture and exercise therapy in patients with mild to moderate CTS. M aterials and Methods: This study was a randomized controlled clinical trial study conducted on 60 patients (55 women) with mild to moderate CTS referring to Imam Hossein Hospital in Tehran in 2017. The patients were randomly divided in to 3 equal groups; groupa1: only night splint was used for 6 weeks (control), group2: splint with tendon and nerve gliding exercises (2 times a day for 6 weeks) were applied, group3: splint and electroacupuncture was performed for 12 sessions (2 sessions/week). The score on the Boston Carpal Tunnel Questionnaire (BCTQ) for Functional Status and Symptom Severity (BCTQ FUNCT and SYMPT) and visual analog scale (VAS) score were evaluated at baseline and after the treatment. R es ults: At the final follow up, significant improvements in all parameters of quality of life and VAS were found in third groups (p<0.05). Acupuncture affected the score on the BCTQ FUNCT and SYMPT, the VAS score more than exercise therapy. In addition, the efficacy of splint alone was less than intervention groups in all parameters. C onclusion: Acupuncture with splint has a high effect on reducing pain and improving the quality of life in patients with mild to moderate CTS and could be adopted in the management of these patients.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"126 1","pages":"201-209"},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76700967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Hundred-Year Efforts by the Pasteur Institute of Iran as a Part of the Rabies Infectious Control Puzzle in the World","authors":"M. Fazeli, A. Fayaz, R. Bashar","doi":"10.22037/NBM.V7I3.26554","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.26554","url":null,"abstract":"Rabies is a fatal zoonotic disease that occurs usually via animal bites. Rabies is currently an economic health problem in Iran and almost all of the provinces are more or less involved in this. For nearly a century, many researchers have tried to control rabies in Iran. To date, Iran has played a major role in the new scientific advances in the rabies treatment field. The Pasteur Institute of Iran was established in a 2.2 ha land to provide public health services for the prevention and treatment of infectious diseases. Since then, the official activity of the Pasteur Institute of Iran has begun. The operations of the Institute are still being developed quantitatively and qualitatively. The aim of this study is to introduce One-Hundred-Year Efforts by the Pasteur Institute of Iran as a Part of the Rabies Infectious Control Puzzle in the World.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"71 1","pages":"165-179"},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75981449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Explaining the structural model of social anxiety based on early maladaptive schemas and family performance","authors":"himan naderzadeh, M. Salehi, M. J. Roshan, R. Entezar","doi":"10.22037/NBM.V7I3.25239","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.25239","url":null,"abstract":"Background: The purpose of study was to determine the mediation role of early maladaptive schemas in the relationship between family performance and social anxiety features of students. Materials and Methods: This study utilized a descriptive correlational design and statistical population of this study was included of all students of Marivan city, with the quantity of 4134 in 2016-2017, through them by an available sampling, 400 students were selected. Research tools were family assessment device by Epstein et al (1983), schema questionnaire- short form by Young (1990), social anxiety by Connor et al (2000). Results: Results showed that early maladaptive schemas mediate the relationship between family performances and social anxiety features (β=0.52, p=0.001). Conclusion: Since parental behaviors is one of the most important affecting factors on early maladaptive schemas and formulation of social anxiety features subsequently; Therefore it is necessary for the prevention and treatment of social anxiety disorder pay special attention to the parent’s behaviors and early maladaptive schemas.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"23 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91492784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship between Shift Work and Burnout among ICU Nursing Staff in Hospitals of Shahid Beheshti University of Medical Sciences","authors":"Shakiba Bakhtom, P. Nassiri, H. Borgheipour","doi":"10.22037/NBM.V7I3.25667","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.25667","url":null,"abstract":"Background: Recent studies have indicated the high prevalence of burnout among nursing staff. Shift work is one of the characteristics of the nursing job; therefore, this study aimed to investigate the relationship between shift work and burnout among intensive care unit (ICU) nursing staff in hospitals of Shahid Beheshti University of Medical Sciences. Materials and Methods: This descriptive-analytic study of cross-sectional type was carried out the period of 2017-2018. The research population was of all nursing staff in the intensive care unit (ICU) in 7 public hospitals and 5 private hospitals. The sample size was 320 nursing staff that was surveyed by census method. To collect data, Maslach burnout inventory (with 78% and reliability and internal validity ranges from 71% to 92 %) and demographic profile questionnaire were used. All statistical analyses analyzed by SPSS software. Results: There was a significant relationship between emotional exhaustion and rotational shift work. There was no significant relationship between emotional exhaustion and night shift. There was a significant relationship between depersonalization and morning shifts in public hospitals. The shift component was only significant in the work shift of the evening and night of public hospitals with personal accomplishment as well. Conclusion: The shift work was considered as an effective factor in the burnout of nursing staff in ICU. By utilizing the results of the study, we can reduce the effects of shift work and make an appropriate intervention plan for promoting staff health.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"57 1","pages":"181-186"},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84103176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of association between flu-like syndrome induced by Beta Interferon drug and required drug response in patients with Multiple sclerosis","authors":"Abdollah Ghaferi, Maziar Shojaei, Arezoo Chouhdari","doi":"10.22037/NBM.V7I3.23085","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.23085","url":null,"abstract":"Background: Beta interferon is one of the important drugs for multiple sclerosis. Its common side effects are flu-like symptoms caused by drug injection. The purpose of this study was to evaluate the possibility of predicting the response rate of this drug based on the flu-like drug reaction. Materials and Methods: This case-control study performed in Loghman Hakim hospital in Tehran in 2017 and 110 patients with multiple sclerosis under beta interferon treatment studied. Patients were divided into two groups with and without flu-like drug reaction. A neurology resident according to the patient’s history and patient records filled in the questionnaires. The results of the two groups were compared by SPSS 16 software. Results: A total of 110 patients including 31 patients with flu-like drug reaction and 79 non-complicated patients were evaluated. These patients included 32 males and 78 females with an average age of 35.55 years. The mean duration of beta interferon use was 4.33 years in the case group and 4.34 years in the control group. Finally, a significant correlation between the flu-like drug reaction and the optimal response in the first year treatment was found (p=0.026). In addition, cause of drug discontinuation had significant correlation with presence of flu like drug reaction (p=0.028). There was no significant correlation between the disease annual attacks rate and flu like drug reaction. Conclusion: Flu-like drug reaction is a common complication of interferon beta drugs, which has a therapeutic difference in patients with and without this drug reaction.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"1 1","pages":"237-245"},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90681262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abdominal pain in opiate addicted patients and other industrial opiates in moddaress hospital 2017-2018","authors":"S. Khorrami, Foroogh Forghani","doi":"10.22037/NBM.V7I3.25283","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.25283","url":null,"abstract":"BackgroundRecognition of abdominal pain help to give better diagnostic and therapeutic approaches and prevention of unnecessary surgeries. The purpose of this study was to determine the causes of abdominal pain in opiate addicted patients and other industrial opiates.Materials and MethodsIn the observational study, a cross-sectional survey was used. A total of 130 consecutive addicted patients and other industrial opiates were enrolled from Modarres Hospital, Tehran, Iran in 2017-2018. Causes of abdominal pain in them were determined and compared across the other variables.ResultsResults of this study demonstrated that the causes of abdominal pain were: defecation problems and constipation,dyspepsia, peptic ulcer disease (PUD), acute abdomen, ileus, obstruction, and biliary problems and others (9.2%, 42.3%, 14.6%, 13.1%, 10%, 7.7%, and 3.1%, respectively).A significant relationship was found between high Lead level and constipation and dyspepsia (P<0.05).ConclusionAccording to the obtained results, it may be concluded that dyspepsia and PUD are most common causes of abdominal pain in addict subjects that is similar to general population and accordingly therapeutic approaches in addict subjects should be similar to the general population.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86557261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-Regulation of Tmevpg1 and Rmrp LncRNA Levels in Splenocytes and Brain of Mouse with Experimental Autoimmune Encephalomyelitis","authors":"E. Farahani, F. Sotoodehnejadnematalahi, Sanaz Mami, Anwar Fathollahi, M. Hajimolahoseini, R. Pouriran, F. Yeganeh","doi":"10.22037/NBM.V7I3.25442","DOIUrl":"https://doi.org/10.22037/NBM.V7I3.25442","url":null,"abstract":"Background: Two long noncoding (lnc) RNAs, which have been recognized as Tmevpg1/Ifng-AS1/NeST and Rmrp play indispensable roles in the differentiation of TH1 and TH17, respectively. The aim of the present scientific study was to analyze the expression levels of the aforementioned lncRNAs in experimental autoimmune encephalomyelitis (EAE) as an animal model for multiple sclerosis (MS).Materials and Methods: Initially, EAE was induced in C57BL/6 mice via immunization by using MOG peptide. The leukocyte infiltration rate and demyelination of neuronal axons were determined. Secondly, the expression levels of Tmevpg1, Rmrp, Tbx21, and Rorc were analyzed in the cultured splenocytes and brain lysates, by using Real-Time PCR assay; eventually, the levels of interferon-gamma and interleukin-17 evaluated by ELISA.Results: Gene expression analysis revealed that Rorc expression in the splenocytes of EAE mice in comparison to the controls was elevated; however, Tbx21 expression did not show any significant difference. Tmevpg1 and Rmrp levels increased in the splenocytes of EAE mice (4.48 times and 39.70 times, respectively, p = 0.0001). Besides, in the brain lysate, the entire genes that have been mentioned were higher than the controls (Tmevpg1: 3.35 times p = 0.02 and Rmrp 11.21 times, p = 0.0001).Conclusion: The marked up-regulation in Tmevpg1 and Rmrp transcripts suggested the essential roles of lncRNAs in the pathogenesis of EAE and multiple sclerosis indeed. Further investigations are necessary to evaluate the values of these lncRNAs as the target for the therapy or molecular marker for disease monitoring.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"44 1","pages":"246-253"},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87558558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}