Nigerian Journal of Natural Products and Medicine最新文献

{"title":"Hepatoprotective effect of methanol seed extract of citrus tangerina on paracetamol-induced hepatotoxicity in Wistar rats","authors":"E. G. Moke, K. Anachuna, K. E. Edje, M. Ojezele","doi":"10.4314/njnpm.v23i1.11","DOIUrl":"https://doi.org/10.4314/njnpm.v23i1.11","url":null,"abstract":"This study investigated the hepatoprotective effect of methanol seed extract of Citrus tangerina on liver damage induced by paracetamol in laboratory rats. Wistar rats were used in this study and categorized into five groups. Groups 1 and 2 received 10 ml/kg normal saline orally, groups 3 and 4 were administered 200 mg/kg and 400 mg/kg respectively of Citrus tangerina seed extract orally, while silymarin 100 mg/kg served as standard drug treatment for group 5. Following six (6) days of pretreatment with the extract, hepatotoxicity was induced with paracetamol 3 g/kg (orally) in all the groups except the positive control group. At the end of the experiment (24 hours after induction), blood samples were collected under diethyl ether anaesthesia for biochemical markers of liver enzymes and antioxidative stress and the liver was harvested for histopathological studies. Both doses (200 mg/kg and 400 mg/kg) of Citrus tangerina seed extract significantly (p < 0.05) reduced the liver enzymes level, but significantly (p < 0.05) increased antioxidant enzymes when compared with the negative control group. Liver histology showed that the Citrus tangerina seed extract prevented hepatic injury induced by paracetamol. The methanol seed extract of Citrus tangerina possesses antioxidative and hepatoprotective effects.","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"57 1","pages":"83-87"},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86939099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical constituents of the stem bark of Acacia AuriculiformisA.Cunn ex. Benth. (fabaceae)","authors":"A. Ahmadu, B. A. Lawal, B. Olanipekun, A. Udobre, N. Tsafantakis, N. Fokialakis","doi":"10.4314/njnpm.v23i1.12","DOIUrl":"https://doi.org/10.4314/njnpm.v23i1.12","url":null,"abstract":"The genus Acacia has been known to be a rich source of many secondary metabolites. This study was carried to isolate chemical constituents present in the stem bark of Acacia auriculiformis. The dichloromethane extract of the stem bark of Acacia auriculiformis was obtained by maceration. The extract obtained was subjected to silica gel column chromatography and preparative TLC. The isolated compounds were identified by spectroscopic analysis. This led to the isolation of ferulic acid ester (I), along with a steroid (II) and a triterpenoid (III). The structure of compound I was established using spectroscopic analysis (UV, IR, NMR and mass spectrometry) and identified to be dodecyl-4-hydroxy-3-methoxy-trans-cinnamate (I), compounds II and III were found to be a-spinasterol and lupenol respectively, based on the comparison of their spectral data NMR and MS with literature report. Compound I is being reported for the first time in the genus Acacia.","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"34 1","pages":"88-91"},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82723630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioassay guided phytochemistry of aerial part of Laportea Aestuans (l.) chew (urticaceae)","authors":"S. Oguntimehin, A. Oriola, E. Obuotor, A. J. Aladesanmi","doi":"10.4314/njnpm.v23i1.2","DOIUrl":"https://doi.org/10.4314/njnpm.v23i1.2","url":null,"abstract":"There are evidences that free radicals mediated damages play important role in the aetiology of several diseases necessitating search for  antioxidants especially from natural origin. Free radical scavenging activities of Laportea aestuans (Urticaceae) is well documented, therefore this study aims at evaluating the safety of L. aestuans and isolating free radical scavenging compound(s) from the plant extract. The aerial (leaves, stem and inflorescence) parts of L. aestuans were collected together and extracted with 80% methanol. The toxicity of the plant extract was evaluated using brine shrimp lethality assay and acute toxicity study while Thin Layer Chromatography (TLC) bioautography with 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH•) radical as detection reagent was used to guide the isolation of compounds from the plant extract. The LC50 of L. aestuans extract was 4276.87 µg/mL, suggesting that the extract is nontoxic. This was further confirmed by the healthiness of study rats following administration of the extract at 5000 mg/kg b.wt in the acute toxicity study. TLC bioautography showed that hexane fraction had the most pronounced radical scavenging activities. Three compounds identified as hexadecanoic acid butyl ester, heptadecanoic and octadecane were characterized using IR, 1H, 13C NMR, ESI – MS and GC – MS analysis.","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"29 1","pages":"6-12"},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80851960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute and sub-acute toxicity of antisickling polyherbal extract in Wistar albino rats","authors":"O. O. Amujoyegbe, M. Idu, J. Agbedahunsi, E. Obuotor, I. Olawuni, O. Oyasowo, G. E. Ogundepo","doi":"10.4314/njnpm.v23i1.6","DOIUrl":"https://doi.org/10.4314/njnpm.v23i1.6","url":null,"abstract":"Piper guineense, Gongronema latifolium and Cymbopogon citratus (PGC) serve as an effective polyherbal antisickling extract used in the  management of sickle cell disorder. This present study assessed the toxicity effect of the ethanol leaf extract of PGC in rats. The acute oral toxicity  test of the polyherbal was evaluated in albino rats using a single-dose based on behavioral changes and mortality. Sub-acute toxicological  evaluation of PGC was examined using biochemical, hematological and histopathological methods. Biochemical analysis was carried out using liver markers enzymes, kidney markers enzymes, and lipid profiles. The hematological measurement includes white blood cell counts (WBC),  Lymphocytes (LYM), monocytes (MON), granulocytes (GRAN), Red blood cell count (RBC). The organs (liver, kidney, and heart) were collected and prepared using standard protocols with hematoxylin and eosin for histopathological evaluation. The acute toxicity study of ethanol leaf extract of PGC up to the limit dose of 2000 mg/kg body weight of the animals used did not produce any signs and symptoms of toxic effects or mortality after 48 hrs. of oral administration. There were no significant differences (p < 0.001) in the observed values between the control and the treated groups for all the biochemical and hematological parameters analyzed. Histopathological evaluation of the organs demonstrated mild degeneration in the kidney and liver while the heart revealed no pathological changes in the treated group of rats. The result of acute toxicity indicates that the  combined antisickling polyherbal PGC extract appeared to be safe and non- toxic. Our findings for the 28 days daily oral administration of PGC extract was dose dependent and well tolerated by the animals. Although, slight changes were observed in some biochemical parameters and histology of the kidney and liver at high doses when compared with control rats. Therefore, the consumption of the antisickling polyherbal PGC extract orally should be used encouraged at lower doses and high doses should be avoided for the management of sickle cell disorder until subjected to further cytotoxicity evaluation. \u0000Keywords: Polyherbal combination, acute toxicity, subacute toxicity, biochemical parameter, hematological parameter, histopathological parameters.","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"48 11","pages":"41-53"},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91498815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemoscopic, macromorphological and micromorphological evaluation of the leaves of Crescentia cujetLinn","authors":"M. B. Olaniyi, I. Lawal, M. I. Adeniyi","doi":"10.4314/njnpm.v23i1.8","DOIUrl":"https://doi.org/10.4314/njnpm.v23i1.8","url":null,"abstract":"The leaves of Crescentia cujete Linn belonging to Bignoniaceae family have been reported to exhibit some important medicinal properties such as antioxidant, antimicrobial, anti-tumour and anti-inflammatory activities. This study therefore evaluated the chemoscopic, macro-morphological andmicromorphological attributes of powdered, fresh and anatomical sections of Crescentia cujete Linn leaves using standard methods. The result ofchemoscopic study confirmed presence of calcium oxalate crystals, fat deposits and traces of lignin in numbers on epidermal surfaces. Macro-morphological study revealed that C. cujete leaves are deep green in colour with faint odour, sour taste and smooth-feel with non-granular  surfaces. Micromorphological examinations indicated that epidermal cells are irregular, rectangular to polygonal in shape on the abaxial surface but epidermal cells on the adaxial surface were straight, slightly undulating with thick anticlinal cell walls. Paracytic stomata arrangement was found only on the abaxial surfaces. Quantitative leaf microscopy confirmed that epidermal cells had average sizes of 37.52 ± 4.62 – 39.23 ± 4.73 μm in  length while cell width was 25.51± 0.86 – 27.76 ± 4.21μm. The stomata length was 15.66 ± 2.04 and 6.29 ±0.93 in width. Organoleptic and other  parameters evaluated on C. cujete shows that it has optimum potential for herbal drug development and a baseline for species identification.","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"1 1","pages":"63-68"},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83044961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute and sub-chronic toxicity studies of aqueous extract of Icacina trichantha Oliv. leaves in rodents","authors":"O. Timothy, I. Igbe, M. Idu, G. Eze, O. Edosuyi","doi":"10.4314/njnpm.v22i1","DOIUrl":"https://doi.org/10.4314/njnpm.v22i1","url":null,"abstract":"This study evaluated the acute and sub-chronic oral toxicity of aqueous extract of the leaves of Icacina trichantha Oliv. The powdered leaves (2 kg) were extracted by decoction in 2 L of distilled water to obtain the aqueous extract. Acute toxicity test was conducted by a single oral administration of the extract in graded dose of 1, 2, 4 and 8 g/kg p.o to four groups of albino mice respectively, while control group received 10 ml/kg p.o. of distilled water. Sub-chronic toxicity test was carried out in Wistar rats of both sexes after daily administration of the extract for 28 days at 100 mg/kg, 500 mg/kg and 1000 mg/kg p.o. Control group received 4 ml/kg p.o. of distilled water. All animals were observed for signs of toxicity and mortality. Changes in body weights, haematology, biochemical parameters, organ to body weight ratio, and organ histology were also examined. Acute and sub-chronic toxicity recorded no mortalities in both male and female animals. Body weight profile and organ histology among experimental groups were not significantly different (P>0.05) compared with control. Platelet counts, serum alkaline phosphatase and relative liver weight index were altered significantly (P 0.05) from the control. There was no significant difference (P>0.05) in electrolytes, urea and creatinine levels. Histopathological studies revealed mild congestion in the heart, liver and lungs, while the kidneys and spleen had no abnormalities. Aside the changes noticed in the platelets and liver, high doses of the aqueous extract of I. trichantha leaves were tolerated in single and repeated 28 days daily administration. However, more specific tests are required to eliminate the possibility of toxicity on liver cells.Keywords: Acute, sub-chronic, toxicity, aqueous extract, Icacina trichantha, leaves","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"2 1","pages":"103-114"},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78408379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative total phenolic content, anti-lipase and antioxidant activities of two Nigerian Aframomum species","authors":"T. Ajayi, J. Moody, I. M. Abiose, N. Ezeoku","doi":"10.4314/NJNPM.V20I1.3","DOIUrl":"https://doi.org/10.4314/NJNPM.V20I1.3","url":null,"abstract":"The anti-obesity drug development is presently not a bright story. So far, drugs reported to be effective have stimulated controversies due to side effects they elicit. Obesity and its co-morbidities continue however to constitute major problems in both developed and developing countries. This has resulted in a continuous search for novel, cost-effective, safe and potent alternatives. This study investigated the ethanolic extracts of two Nigerian Aframomum species for their anti-lipase and anti-oxidant activities as well as estimates of their polyphenol contents. Lipase activity was determined using glyceryltrioleate emulsion as a substrate and measuring the release rate of oleic acid from it. Percentage inhibition of lipase by the methanolic extracts of plants was determined spectrophotometrically at T 0 and T 30 (30 minutes after incubation at 37 o C). DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity of the extracts and that of gallic acid as control was measured using the stable radical DPPH method and absorbance at 515 nm using a spectrophotometer. The IC 50 (half-maximal inhibitory concentration) value was calculated by linear regression analysis and the total phenolic content was determined by the Folin-Ciocalteau method at 765 nm. The standard curve was prepared by solutions of Gallic acid in methanol: water (50:50, v/v). Total phenol values are expressed in terms of Gallic acid equivalent (w/w of dry mass). Aframomum melegueta exhibited the highest phenolic content of 60.4 ± 2.36 mgGAE/g, a percentage antioxidant activity of 86.6 % at 200μg/ml and percentage lipase inhibition of 89% at 1mg/ml while Aframomum danielli revealed a total phenolic content of33.3 ± 2.71mgGAE/g, a percentage antioxidant activity of 77.3% at 200μg/ml and percentage lipase inhibition of 73% at 1 mg/ml. The result provides some justifications for the use of these plants in ethno-medicine for the management of obesity. The species exhibited properties that are beneficial to health and therefore could find use as an alternative and/or complementary strategy in managing associated co-morbidities of obesity, and also as possible template for future anti-obesity drug development. Keywords: Pancreatic lipase, Aframomum , Orlistat ® , Obesity","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"13 1","pages":"12-19"},"PeriodicalIF":0.0,"publicationDate":"2017-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76089413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antisickling and antioxidant properties of aqueous and ethanol extracts of fifty selected plants used in the management of sickle cell disorder in southern Nigeria","authors":"O. O. Amujoyegbe, M. Idu, J. Agbedahunsi, E. Obuotor, G. Bazuaye","doi":"10.4314/njnpm.v20i1.23","DOIUrl":"https://doi.org/10.4314/njnpm.v20i1.23","url":null,"abstract":"Sickle Cell Disease (SCD) is an ailment with enormous social and economic burden for patients and care givers. The study evaluated the in vitro antisickling and antioxidant properties of aqueous and ethanol extracts of fifty selected plants used in the management of sickle cell disease (SCD) with the aim of justifying their use in the management of the disease in southwestern Nigeria. Aqueous and 70% ethanol extracts of fifty (50) surveyed plants were subjected to in vitro antisickling activities and forty plants with above 50% activity levels in both inhibitory and reversal models were later tested for their antioxidant assay involving four tests namely DPPH, FRAP, Fe-chelating and total antioxidant content using standard methods. Significant mean values were separated using the Least Significant Difference at 0.05 % level of probability. Among all the plants with above 50% activity levels in both inhibitory and reversal models, three plants which are Gongronema latifolium , Cymbopogon citratus and Piper guineense had the highest value of 89.81, 89.72 and 84.48 % respectively in ethanol extracts. The least activity for both aqueous and ethanol extracts was found in Amaranthus spinosus and Amaranthus viridis . It can be inferred from the result of the study that 80 % the plants evaluated possessed high antisickling and antioxidant activities and may thus justified their use for the management of SCD in the South – West, Nigeria. Keywords: Sickle cell disease, antisickling, inhibition, reversal, antioxidant","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"12 1","pages":"146-160"},"PeriodicalIF":0.0,"publicationDate":"2017-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87771867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of the areas of specialization of Traditional Medicine practitioners (TMPs) on the types of formulation prescribed in the management of memory loss","authors":"J. Oiseoghaede, G. Ajayi, O. Odukoya, A. Sowemimo, N. F. Mustapha","doi":"10.4314/njnpm.v20i1.6","DOIUrl":"https://doi.org/10.4314/njnpm.v20i1.6","url":null,"abstract":"Traditional Medicine Practitioners (TMPs) make use of plants in alleviation of many illnesses including memory loss. Their specialization categories could include traditional healers, herbalists, herb sellers, etc. Most of them learnt the trade as apprentices from their trainers or their parents. This study was designed to investigate if there was an association between the areas of specialization of the TMP and the choice of formulation (mono- or multicomponent herbal) in the management of memory loss. A hundred TMPs consisting of 18 traditional healers and 82 herb sellers in Lagos State were randomly interviewed using a structured questionnaire. Various data on age, sex, areas of specialization of the TMPs, plants used, mode of administration, life forms of plants used and the types of herbal formulation prescribed for memory loss were obtained. The two variables involved (areas of specialization of the TMPs and formulation type) were compared using Chi square statistical tool to measure their association and inferences were drawn from the results. About 88% of all respondents have used multi-component herbal formulations while only 12% have used mono-component herbal formulations. About 82% of all the respondents were herb sellers and 18% of them were traditional healers. About 89% of literate respondents have used multi-component herbal formulations while 11% of them have used mono-component herbal formulations. Similarly, about 89% of herb sellers have used multi-component herbal formulations while about 11% of them have used mono-component herbal formulations. About 83% of traditional healers have used multi-component herbal formulations while about 17% of them have used mono-component herbal formulations. There was no significant association between the types of occupation of the TMPs interviewed and the types of formulation used (p< 0.05). This study shows that the occupations (trades) of TMPs did not influence their choice of type of formulations. Keywords:  Mono-component herbal, multi-component herbal, traditional medicine practitioners, occupation","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"1 1","pages":"35-36"},"PeriodicalIF":0.0,"publicationDate":"2017-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90924584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary antifertility activity of Bambusa vulgaris leaf extract in male wistar rats","authors":"G. Alade, T. Alade, Olanrewaju R Awotona, J. Moody","doi":"10.4314/NJNPM.V20I1.11","DOIUrl":"https://doi.org/10.4314/NJNPM.V20I1.11","url":null,"abstract":"The aqueous extract of the leaf of Bambusa vulgaris is used in ethnomedicine as an antifertility agent in man. This study aimed at validating or disproving this folkloric use by evaluating the effect of 50 % methanolic extract on sperm concentration and some male reproductive hormones in rats after 14 and 28- day administration. Male wistar rats were administered with 250 and 500 mg/kg of the extract for 14 and 28 days and were sacrificed at the end of these days separately. They were evaluated for sperm concentration, motility, testosterone (T), leutinizing (LH) and follicle stimulating hormones (FSH), and histology of the testes was also carried out. Two groups of six rats each administered with 250 mg/kg for 28 days were left for the next 14 and 28 days, respectively and sperm concentration and motility were evaluated for probable reversal of activity. The same was repeated for those two groups administered with 500 mg/kg extract. There were 42 and 31 % reduction in sperm count at 14 and 28 days respectively, in rats administered with 250 mg/kg while at 500 mg/kg dose, the percentage reduction in sperm count was 60 % at both 14 and 28 days. There was almost a complete reversal of activity 14 days after cessation of treatment. The result justified the ethnomedicinal claim of the use of B. vulgaris leaf as an antifertility agent. Keywords: Bamboo, sperm, contraception, fertility, hormone","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"256 1","pages":"61-66"},"PeriodicalIF":0.0,"publicationDate":"2017-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82944119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}