Nucleosides, Nucleotides & Nucleic Acids最新文献_第6页

The role of miR-1180-3p in breast cancer and its effect on the cell functions of breast cancer cells. miR-1180-3p在乳腺癌中的作用及其对乳腺癌细胞功能的影响。

IF 1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-12-09 DOI: 10.1080/15257770.2025.2580336

Jiayuan Mao, Yinxiang Xu, Hongxia Lin, Weixia Zhou, Man Tang

{"title":"The role of miR-1180-3p in breast cancer and its effect on the cell functions of breast cancer cells.","authors":"Jiayuan Mao, Yinxiang Xu, Hongxia Lin, Weixia Zhou, Man Tang","doi":"10.1080/15257770.2025.2580336","DOIUrl":"10.1080/15257770.2025.2580336","url":null,"abstract":"Objective: This investigation sought to characterize the clinical relevance and functional impact of miR-1180-3p in breast cancer (BRCA) pathogenesis while delineating its underlying mechanistic basis.Methods: MiR-1180-3p expression in tumor tissues from 139 BRCA patients and cells was assessed by using polymerase chain reaction (PCR). Correlation analysis evaluated its association with clinicopathological characteristics. In vitro, the functional consequences of miR-1180-3p expression on cell proliferation, migration, and invasion of MCF7 and MDA-MB-231 were determined by Cell Counting Kit-8 and Transwell assay. Bioinformatic analysis predicted miR-1180-3p target genes, with dual luciferase reporter assays validated the interaction. Rescue studies were conducted to confirm the effect of the miR-1180-3p/ZNF83 axis on BRCA cell functions.Results: Significant miR-1180-3p downregulation was observed in BRCA. Low miR-1180-3p levels correlated with poor prognosis and shorter survival, and were associated with ultrasound features like tumor size and lymph node metastasis. Functional studies showed miR-1180-3p overexpression inhibited BRCA cell proliferation, migration, and invasion, while its downstream target gene ZNF83 exhibited significantly elevated expression levels in BRCA cells, and overexpression of ZNF83 abrogated miR-1180-3p-mediated suppression of proliferative, migratory, and invasive capacities in BRCA cells.Conclusion: In summary, miR-1180-3p served as a prognostic biomarker for BRCA and regulated cellular functions, thereby being involved in tumor progression.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1070-1085"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ELF3-AS1 promotes the carcinogenesis of hepatocellular carcinoma cells by inhibiting miR-98-5p/CPSF4 axis. ELF3-AS1通过抑制miR-98-5p/CPSF4轴促进肝癌细胞的癌变。

IF 1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-11-24 DOI: 10.1080/15257770.2025.2590583

PengXin Ge, Sensen Niu, Min Fang, Qianru Xu, Wei Zhang, Jiliang Xu, Feng Yang, Yangkui Wang, Tianlu Shi, Hongjin Liu

{"title":"ELF3-AS1 promotes the carcinogenesis of hepatocellular carcinoma cells by inhibiting miR-98-5p/CPSF4 axis.","authors":"PengXin Ge, Sensen Niu, Min Fang, Qianru Xu, Wei Zhang, Jiliang Xu, Feng Yang, Yangkui Wang, Tianlu Shi, Hongjin Liu","doi":"10.1080/15257770.2025.2590583","DOIUrl":"10.1080/15257770.2025.2590583","url":null,"abstract":"Hepatocellular carcinoma (HCC), which predominantly manifests as a malignant form of primary liver cancer, remains resistant to existing therapies in many patients. Therefore, elucidating the cellular processes and signaling networks driving HCC progression and discovering novel treatment targets remain key areas of research. ELF3-AS1, miR-98-5p, and CPSF4 are closely associated with liver cancer, but the relationship between them is unclear. ELF3-AS1, miR-98-5p, and CPSF4 levels were quantified using RT-qPCR. CPSF4 expression was analyzed through Western blotting. Liver tumor cell viability was assessed using CCK8 assays. The metastatic potential of cells was primarily evaluated using wound healing and Transwell assays. ELF3-AS1's impact on tumors was validated through live animal experiments by inducing subcutaneous tumor formation in nude mice. RNAhybrid and TargetScan analyzed potential miR-98-5p target regions in CPSF4 and ELF3-AS1. Our study demonstrates that reducing ELF3-AS1 expression can inhibit hepatoma cell proliferation, migration, and invasive abilities. Specifically, knocking down ELF3-AS1 can reduce the expression of CPSF4. Knocking down ELF3-AS1 can suppress liver cancer development in live animal models. In addition, miR-98-5p can bind ELF3-AS1 and CPSF4 and down-regulate the expression of CPSF4. In summary, ELF3-AS1 promotes the proliferation, migration, and invasion of HCC cells by inhibiting miR-98-5p/CPSF4 axis.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1086-1105"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145588496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precise control model of the epidemic: a cross-sectional study. 流行病的精确控制模型：横断面研究。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-03 DOI: 10.1080/15257770.2025.2470736

Mingyun Jiang, Ruiqi Liu, Weiping Yao, Shuang Li, Xiaodong Liang, Haibo Zhang

{"title":"Precise control model of the epidemic: a cross-sectional study.","authors":"Mingyun Jiang, Ruiqi Liu, Weiping Yao, Shuang Li, Xiaodong Liang, Haibo Zhang","doi":"10.1080/15257770.2025.2470736","DOIUrl":"10.1080/15257770.2025.2470736","url":null,"abstract":"Objectives: Coronavirus disease 2019 (COVID-19) is the most influential public health emergency worldwide. Controlling viral infection with people's health and reducing the impact on people's freedom is difficult at present. The precise control of COVID-19 in a city may be a suitable solution.Methods: An anonymous cross-sectional survey was conducted among Hangzhou people between 1 January and 28 February 2022. We organized the classification, incidence rate and mortality of COVID-19. And we introduced the discovery process of Omicron, health code of four colors, the epidemiological investigation and the policies of government in Hangzhou. This paper discusses various measures taken against Omicron in Hangzhou, China, which are effective methods to deal with such public health emergencies.Conclusions: Hangzhou quickly controlled the epidemic through precise control. As of February 1, Hangzhou had 115 confirmed cases with total population is 12.204 million. The rate of severe and death is 0%. Hangzhou's new model of precise control provides an important reference for the global city's response to COVID-19 and the reduction in losses caused by COVID-19. The COVID-19 Omicron variant outbreak indicates that people will still face unpredictable health risks in the future. Precise control is one of the best ways to effectively manage an epidemic, minimize its severity, and reduce losses in all aspects.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"575-588"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ACE I/D and ATIR A1166C variants in patients with diabetes mellitus with and without peripheral neuropathy in Turkish patients. 土耳其伴有和不伴有周围神经病变的糖尿病患者中ACE I/D和ATIR A1166C变异的评估

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-01-17 DOI: 10.1080/15257770.2025.2451382

Payam Amiri Dashatan, Huseyin Soylu, Mehmet Elbistan, Aysegul Atmaca, Adem Keskin, Zulfinaz Betul Celik, Serbulent Yigit

{"title":"Evaluation of ACE I/D and ATIR A1166C variants in patients with diabetes mellitus with and without peripheral neuropathy in Turkish patients.","authors":"Payam Amiri Dashatan, Huseyin Soylu, Mehmet Elbistan, Aysegul Atmaca, Adem Keskin, Zulfinaz Betul Celik, Serbulent Yigit","doi":"10.1080/15257770.2025.2451382","DOIUrl":"10.1080/15257770.2025.2451382","url":null,"abstract":"Objective: Type 2 Diabetes Mellitus (T2DM) can lead to long-term vascular complications such as diabetic peripheral neuropathy (DPN). This study aimed to investigate the role of angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) and angiotensin II type 1 receptor (AT1R) A1166C variants in the predisposition to T2DM in the Turkish population and their association with DPN.Methods: The study included 90 T2DM patients (42 with DPN) and 50 healthy individuals. ACE I/D and ATIR A1166C gene regions were analyzed for the variant. Both the general genotype distribution of these variants and the observed genotype ratios were examined separately.Results: In the T2DM group, the proportion of individuals with the AA genotype of the AT1R A1166C variant was lower than in the control group, and the proportion of individuals with the AC genotype was higher. There was no significant difference in the genotype distribution between the groups for the ACE I/D variant. There was no significant difference in the genotype distribution of the ACE I/D and ATIR A1166C variants in patients with and without DPN.Conclusion: In the Turkish population, no significant difference was observed in the overall genotype distribution of ACE I/D and AT1R A1166C variants between T2DM patients and healthy individuals, whereas the AC genotype of the AT1R A1166C variant was more frequent in T2DM patients, and the AA genotype was less frequent. For both variants, no significant difference was observed in the genotype distribution between T2DM patients with and without DPN.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"103-112"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of MBL2 gene polymorphisms with type 2 diabetes and its complications in Moroccan population. 摩洛哥人群中MBL2基因多态性与2型糖尿病及其并发症的关系

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-02-17 DOI: 10.1080/15257770.2025.2466429

Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Wajih Rhalem, Lahcen Wakrim, Imane Ettaki, Hassan Ghazal, Najib Al Idrissi, Omar Abidi, Fadel Bakkali, Abderrahim Naamane, Naima Khlil, Salsabil Hamdi

{"title":"Association of MBL2 gene polymorphisms with type 2 diabetes and its complications in Moroccan population.","authors":"Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Wajih Rhalem, Lahcen Wakrim, Imane Ettaki, Hassan Ghazal, Najib Al Idrissi, Omar Abidi, Fadel Bakkali, Abderrahim Naamane, Naima Khlil, Salsabil Hamdi","doi":"10.1080/15257770.2025.2466429","DOIUrl":"10.1080/15257770.2025.2466429","url":null,"abstract":"The MBL2 gene encodes the mannose-binding lectin protein (MBL), which is secreted by the liver. Several variants of MBL2 have been found to be associated with altered serum levels and susceptibility to various chronic diseases. Defects in MBL protein polymerization that result in functional impairments and/or low serum levels may influence genetic susceptibility to type 2 diabetes (T2D) and its complications. Therefore, the present case-control study was conducted to assess the potential association of six MBL2 gene variants and haplotypes with susceptibility to T2D and its complications in Morocco. The MBL2 gene was genotyped by PCR-sequencing for the promoting, non-coding, and coding regions in 435 individuals. Our findings revealed a significant association between the heterozygous CG and homozygous recessive GG genotypes of the variant at position -221 C > G in the MBL2 gene promoter with an increased risk of T2D. Similarly, for +4 C > T in the non-coding region, statistical analysis indicates a strong association with T2D risk, particularly with the heterozygous CT and homozygous recessive TT genotypes. The LYQC haplotype is also found to be associated with T2D risk. Furthermore, the heterozygous CT genotype, and recessive T allele of the variant at position +4 C > T, and heterozygous GA genotype of codon Gly54Asp of the MBL2 gene, are associated with protection against hypertension in T2D patients. However, no association was observed between MBL2 variants and dyslipidemia in T2D patients. The study concludes that -221 C > G and +4 C > T variants of the MBL2 gene significantly contribute to T2D susceptibility in Morocco.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"131-152"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable synthesis of benzimidazole-based Schiff base using reusable CaAl₂O₄ nanophosphors catalyst: Insights into metal(II) complexes and DNA interactions. 使用可重复使用的CaAl2O4纳米磷光催化剂可持续合成苯并咪唑基希夫碱：金属（II）配合物和DNA相互作用的见解。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-01-19 DOI: 10.1080/15257770.2025.2451375

M Manjunath, F H Sujata, A H Shridhara, B Vinay Kumar, K Prashantha, K Yogendra, N Madhusudhana

{"title":"Sustainable synthesis of benzimidazole-based Schiff base using reusable CaAl2O4 nanophosphors catalyst: Insights into metal(II) complexes and DNA interactions.","authors":"M Manjunath, F H Sujata, A H Shridhara, B Vinay Kumar, K Prashantha, K Yogendra, N Madhusudhana","doi":"10.1080/15257770.2025.2451375","DOIUrl":"10.1080/15257770.2025.2451375","url":null,"abstract":"This article presents a new and facile method for the synthesis of Schiff base compounds with a benzimidazole group using a low-cost and reusable calcium aluminate nanophosphorus catalyst (CaAl2O4). This approach avoids harmful solvents and reactants, supporting a more environmentally friendly synthesis process. The catalyst maintained its activity and heterogeneity over four cycles with minimal loss of efficiency. The synthesis process was straightforward and eliminated the need for column chromatography. The Schiff base ligand (HL=(E)-N-((6-(thiophen-2-yl)pyridin-2-yl)methylene)-1H-benzo[d]imidazol-2-amine)) was synthesized by the reaction of 6-(thiophen-2-yl)pyridine-2-carbaldehyde with 1H-benzimidazole-2-amine. Subsequently, metal(II) complexes of Co(II), Ni(II), and Cu(II) were prepared using this ligand. Structural analysis of both the ligand and its metal complexes was carried out using various physicochemical and spectroscopic methods. Ni(II) and Co(II) complexes were found to adopt an octahedral geometry, while the Cu(II) complex exhibited a square-planar structure. Binding studies with calf thymus DNA (CT-DNA) at pH 7.2 were performed using UV-visible spectroscopy, viscosity measurements, and thermal denaturation studies and showed that the metal complexes intercalate into the DNA and produced a distinct binding pattern. Molecular docking simulations with AutoDock Vina provided insights into the interaction of these complexes with the B-DNA dodecamer. Furthermore, the ligand and its metal complexes showed UV-visible photonuclease activity against pUC19 DNA. Agarose gel electrophoresis showed that the metal complexes exhibit photoinduced nuclease activity, confirming their ability to cleave DNA upon exposure to light.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"238-260"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano calcium zincate-assisted synthesis of benzo[d]thiazol-2-yl phenylisoxazoles: quantum computational, in silico molecular docking simulations and DNA interaction. 纳米锌酸钙辅助合成苯并[d]噻唑-2-基苯基异恶唑：量子计算、硅分子对接模拟和DNA相互作用。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-06 DOI: 10.1080/15257770.2025.2473442

A K Smitha, V Srinivasa Murthy, B Vinay Kumar, M Sennappan, A H Shridhar, Lohit Naik, K Yogendra, N Madhusudhana

{"title":"Nano calcium zincate-assisted synthesis of benzo[d]thiazol-2-yl phenylisoxazoles: quantum computational, in silico molecular docking simulations and DNA interaction.","authors":"A K Smitha, V Srinivasa Murthy, B Vinay Kumar, M Sennappan, A H Shridhar, Lohit Naik, K Yogendra, N Madhusudhana","doi":"10.1080/15257770.2025.2473442","DOIUrl":"10.1080/15257770.2025.2473442","url":null,"abstract":"This study introduces a new and simple method for the synthesis of a series of 3-(benzo[d]thiazol-2-yl)-5-phenylisoxazole derivatives 3(a-f), and examines its potential interactions with DNA. The synthesis includes the reaction of 2-aminobenzenethiol (1) with a variety of substituted 5-phenylisoxazole-3-carbaldehydes 2(a-f) in the presence of a cost-effective and reusable nanocatalyst, Calcium-Zincate (CaZnO2). The CaZnO2 catalyst showed a consistent and long-lasting catalytic activity over several reaction cycles and retained its unique heterogeneous properties. The resulting compounds were characterized in detail using various spectroscopic and analytical techniques in order to confirm their structures. In addition, the interaction of these synthesized compounds with calf thymus-DNA (CT-DNA) using absorption spectroscopy and viscosity measurements was assessed. In silico docking studies were performed to predict their binding affinity with human DNA (PDB ID: 1G3X). The compounds were further analyzed using the Density Functional Theory (DFT) with the B3LYP functional and the 6-31 G(d) basis set in chloroform, with the results aligning closely with the experimental findings. Furthermore, the compounds ability to cleave PUC19 DNA was assessed, along with their photoinduced nuclease activity under UV-visible light, confirmed by photo-induced cleavage assays.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"261-286"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-766-3p promotes non-small cell lung cancer development through suppression of NR3C2. miR-766-3p通过抑制NR3C2促进非小细胞肺癌的发展。

IF 1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-07-20 DOI: 10.1080/15257770.2025.2533391

Xuexi Zhang, Lei Cao, Gang Zhang

{"title":"miR-766-3p promotes non-small cell lung cancer development through suppression of NR3C2.","authors":"Xuexi Zhang, Lei Cao, Gang Zhang","doi":"10.1080/15257770.2025.2533391","DOIUrl":"10.1080/15257770.2025.2533391","url":null,"abstract":"The incidence of non-small cell lung cancer (NSCLC) has exhibited an elevated trend yearly, seriously threatening human health. However, its molecular mechanism is still unknown. The objective of this experiment was to investigate the expression and prognostic value of miR-766-3p in the tissues of NSCLC patients, as well as to provide possible targets for the healing of NSCLC. In this study, miR-766-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2) were detected in tissues of NSCLC patients using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Survival analysis was estimated with Kaplan-Meier and Cox proportional hazards model. In vitro experiments included Cell Counting Kit-8 (CCK-8) viability assay, flow cytometry for apoptosis, Transwell assay for migration and invasion, and luciferase reporter assay for target genes. miR-766-3p levels were clearly elevated in tumor tissues, and high miR-766-3p levels were considered to be a poor prognostic factor. NR3C2 was clearly down-regulated in the serum of NSCLC patients. miR-766-3p overexpression stimulated the proliferation and enhanced metastatic spread of lung cancer cells, whereas down-regulation of NR3C2 reversed the effect of miR-766-3p on cellular activity. miR-766-3p promotes NSCLC development by inhibiting NR3C2 levels and is a potential biomarker. Furthermore, high levels of miR-766-3p are likely to predict poor prognosis in NSCLC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"838-848"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A positive correlation between the pseudorotational phase angle P and the δ_H torsion angle (H4'-C4'-C3'-H3') in nucleosides and nucleic acids. 核苷和核酸的赝旋相角P与δH扭角（H4′-C4′-C3′-H3′）呈正相关。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-06-11 DOI: 10.1080/15257770.2025.2516597

Jesse Vanloon, Alexander Y Yan, Hongbin Yan

引用次数: 0

Biophysical and electrochemical studies on the interaction of arbutin drug with calf-thymus DNA. 熊果苷类药物与小牛胸腺DNA相互作用的生物物理和电化学研究。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-06-02 DOI: 10.1080/15257770.2025.2512857

D S Bhuvaneshwari, Kandasamy Pavithra, Kuppanagounder P Elango

{"title":"Biophysical and electrochemical studies on the interaction of arbutin drug with calf-thymus DNA.","authors":"D S Bhuvaneshwari, Kandasamy Pavithra, Kuppanagounder P Elango","doi":"10.1080/15257770.2025.2512857","DOIUrl":"10.1080/15257770.2025.2512857","url":null,"abstract":"Understanding the interaction of therapeutic drugs with DNA is crucial for designing highly selective DNA-targeted medicines that could overcome the current therapeutic limitations. In this endeavour, the DNA binding behaviour of arbutin (ATN) was explored using multi-spectroscopic, electrochemical and computational studies. The UV-Vis spectral studies authenticated the complexation of ATN with CT-DNA and exposed ATN as a moderately strong DNA binder with a binding constant of 8.029 × 103 M-1. The findings of fluorescence spectral studies not only revealed the spontaneous ground state complex formation between ATN and CT-DNA, but also emphasised the role of hydrogen bonding and Van der Waals interactions in stabilising the ATN/CT-DNA complex. Since the competitive dye displacement assay strongly excluded the plausibility of classical intercalation and conventional groove binding mode of ATN, viscosity studies provided clues regarding the external binding mode of ATN. The appreciable enhancement resulted in the fluorescence emission of the ATN/CT-DNA complex upon increasing NaCl concentration, which certified ATN as an external binder. The CD spectral results exposed the ATN-induced moderate conformational alterations in CT-DNA. Remarkably, the voltammetric titration results labelled the glucopyranoside moiety of ATN as a DNA binding unit with a formation constant of 2.57 × 104 M-1 rather than the hydroquinone moiety of ATN. Molecular docking and metadynamics simulation outcomes served as pictorial evidence of experimental results. They revealed the predominant contribution of hydrogen bonding interactions in stabilising ATN/DNA complexation.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"666-688"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0