Neuroscience Letters最新文献_第2页

Bilateral transport of neuronal tracer after unilateral administration: Investigation of potential peripheral mechanism

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-21 DOI: 10.1016/j.neulet.2025.138219

Jaime Fabillar Jr. , Lutfi Putra Perdana , Swarnalakshmi Raman , Junhel Dalanon , Masamitsu Oshima , Otto Baba , Yoshizo Matsuka

{"title":"Bilateral transport of neuronal tracer after unilateral administration: Investigation of potential peripheral mechanism","authors":"Jaime Fabillar Jr. , Lutfi Putra Perdana , Swarnalakshmi Raman , Junhel Dalanon , Masamitsu Oshima , Otto Baba , Yoshizo Matsuka","doi":"10.1016/j.neulet.2025.138219","DOIUrl":"10.1016/j.neulet.2025.138219","url":null,"abstract":"<div><div>Previous studies have shown pain attenuation after the administration of botulinum neurotoxin type A into the whisker pad contralateral to the nerve injury. It further showed localization in the neurons of the bilateral trigeminal ganglia, indicating a possible connection between the trigeminal nerves. Thus, this study aimed to investigate the potential link between the right and left trigeminal ganglia, considering their anatomical proximity and the possible neural interactions between these structures. The fluorogold (FG) neuronal tracer was localized in the neurons of both the right and left trigeminal ganglia (TG) after unilateral injection into the whisker pad. In contrast, there was a substantial decrease in FG localization in the contralateral ganglion following colchicine (CCh) administration or after midline incision surgery. Moreover, FG injected directly into the trigeminal ganglion was exclusively localized on the ipsilateral side. Additionally, systemic administration of FG revealed its localization not only within the bilateral TG neurons but also in the bilateral dorsal root ganglia (DRG) neurons, mirroring the findings following whisker pad injection. These findings suggest that bilateral transport of fluorogold occurs at the peripheral level, possibly through its diffusion from the injection site to the contralateral whisker pad and/or nerve axonal crossover through the midline. Further studies are required to clarify the specific peripheral pathways and explore the bilateral nerve connections.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"854 ","pages":"Article 138219"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of G6PD activity with a modified hormonal environment during the critical period of hypothalamic sexual differentiation in Sprague Dawley rats

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-21 DOI: 10.1016/j.neulet.2025.138206

Chávez García Ricardo , Ortega Camarillo Clara , Contreras Ramos Alejandra , Díaz Rosas Guadalupe , Avalos Rodríguez Alejandro , J.J. Pérez-Rivero , Vergara Onofre Marcela

{"title":"Comparison of G6PD activity with a modified hormonal environment during the critical period of hypothalamic sexual differentiation in Sprague Dawley rats","authors":"Chávez García Ricardo , Ortega Camarillo Clara , Contreras Ramos Alejandra , Díaz Rosas Guadalupe , Avalos Rodríguez Alejandro , J.J. Pérez-Rivero , Vergara Onofre Marcela","doi":"10.1016/j.neulet.2025.138206","DOIUrl":"10.1016/j.neulet.2025.138206","url":null,"abstract":"<div><div>The neuronal cells that make up the dysmorphic nuclei located in the hypothalamus of both female and male mammals are susceptible to the hormonal environment in which individuals find themselves during the critical period of cerebral sexual differentiation. Thus, endogenous and exogenous steroids influence the remodeling process of hypothalamic structures using apoptosis or cell proliferation processes, which are implicit in the mechanism of cerebral sexual differentiation. In this study, the enzymatic kinetics and the relative expression of G6PD mRNA and the concentration of GSH were compared to verify whether the redox state modified by the exogenous administration of synthetic hormones, such as tamoxifen in males and testosterone propionate in females, influences the apoptosis process (Bax and Bcl-2) within the mechanism of hypothalamic sexual differentiation in Sprague Dawley rats. It was observed that the G6PD activity for the F-CTR group was higher at 1 and 3 h compared to the M-TAM organisms. The F-TP group registered more significant values at 3 h compared to the M-CTR group. For the GSH concentrations, the F-CTR and M-CTR groups exhibited the highest concentrations at 12, 24, and 48 h. In the experimental groups, the GSH concentrations remained at decreased levels from the first hour to 48 h with a slight increase at 3600 h in the F-TP. In the case of the M-TAM, the GSH levels rose from hour 3 to hour 12.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"855 ","pages":"Article 138206"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct movement related changes in EEG and ECeG power during finger and foot movement 在手指和脚的运动过程中，脑电图和心电图功率会发生与运动相关的明显变化。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-19 DOI: 10.1016/j.neulet.2025.138207

Neil P.M. Todd , Sendhil Govender , Daniel Hochstrasser , Peter E. Keller , James G. Colebatch

{"title":"Distinct movement related changes in EEG and ECeG power during finger and foot movement","authors":"Neil P.M. Todd , Sendhil Govender , Daniel Hochstrasser , Peter E. Keller , James G. Colebatch","doi":"10.1016/j.neulet.2025.138207","DOIUrl":"10.1016/j.neulet.2025.138207","url":null,"abstract":"<div><div>Voluntary movement is known to be associated with cerebrally generated movement-related slow potentials and parallel changes in spectral power. The cerebellar and cerebral cortices are powerfully connected via reciprocal, crossed projections which mediate their coordination in motor, as well as cognitive and affective processes. The cerebellum participates in movement and the question remains as to the nature of movement related changes in power if any which might occur over the cerebellum. In a sample of six healthy adult subjects, we recorded EEG and the electro-cerebellogram (ECeG) with a 10 % cerebellar extension montage during voluntary left and right index finger and foot movements. EMG was recorded from finger extensors and flexors and from the tibialis anterior and soleus muscles and was used to generate triggers for movement related averaging (−2000 to + 2000 ms). Wavelet power was computed over the 4 s epoch for each electrode. For statistical analysis, cerebral and cerebellar grids centred around Cz and SIz were investigated, using both average and linked-ear references. Statistically significant movement related changes were observed in both cerebral and cerebellar power, most significantly in the high delta band (1.5 to 3 Hz), for both montages. In contrast to Cz where power increased premovement, power was reduced over the cerebellum. High-frequency pause-bursting was also observed in the ECeG around the time of movement. Our results indicate that recordings over the cerebellum show distinct changes from those over Cz and in particular show a fall in power during the premovement period.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"853 ","pages":"Article 138207"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collateral projections from the lateral parabrachial nucleus to the bed nucleus of the stria terminalis and the central amygdala in mice

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-18 DOI: 10.1016/j.neulet.2025.138204

Li Zhang , Shuai Zhou , Yuen Fen Tan , Quan Fu Gan , Teoh Hoon Koon , Zhiqiang Wang , Shiqing Zhao , Yixuan Chen , Yi Sun , Pooi Pooi Leong

{"title":"Collateral projections from the lateral parabrachial nucleus to the bed nucleus of the stria terminalis and the central amygdala in mice","authors":"Li Zhang , Shuai Zhou , Yuen Fen Tan , Quan Fu Gan , Teoh Hoon Koon , Zhiqiang Wang , Shiqing Zhao , Yixuan Chen , Yi Sun , Pooi Pooi Leong","doi":"10.1016/j.neulet.2025.138204","DOIUrl":"10.1016/j.neulet.2025.138204","url":null,"abstract":"<div><h3>Background</h3><div>The lateral parabrachial nucleus (LPBn) serves a critical hub for processing and transmitting pain signals. It has two main efferent pathways, the LPBn-CeA and LPBn-BNST, which are crucial for pain regulation and the management of negative emotions.</div></div><div><h3>Methods</h3><div>In our study, we investigated the projections from the LPBn by performing stereotaxic injections of AAV2/2Retro-hSyn-EGFP (abbreviated as EGFP) into the bed nucleus of the stria terminalis (BNST) and AAV2/2Retro-hSyn-tdTomato (abbreviated as tdTomato) into the central amygdala (CeA) in mice. The animals subsequently underwent spared nerve injury (SNI) surgery on the contralateral side to the AAV injections. To examine the expression of calcitonin gene-related peptide (CGRP) and c-Fos, we conducted immunofluorescent histochemistry.</div></div><div><h3>Results</h3><div>Our results indicated that approximately 26 % of the LPBn neurons retrogradely labeled with either EGFP or tdTomato were dual-labeled with both markers. Moreover, a significant majority (85.49 %) of these double-labeled neurons were CGRP positive (CGRP<sup>+</sup>). In mice subjected to SNI, nearly all of these neurons (93.25 %) were c-Fos positive (c-Fos<sup>+</sup>), indicating that they were activated.</div></div><div><h3>Conclusion</h3><div>These findings suggest that a subset of CGRP<sup>+</sup> neurons in the LPBn projects to both the BNST and CeA via axon collaterals. Notably, under SNI conditions, these neurons may play a critical role in the transmission of chronic pain.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"853 ","pages":"Article 138204"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of atrial natriuretic peptide receptor A depresses hypothalamic PVN CRFergic neuronal excitability by inhibiting IH in vitro in mice

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-17 DOI: 10.1016/j.neulet.2025.138202

Yong-Rui Piao , Yang Liu , Chun-Ping Chu , Yu-Zi Li , De-Lai Qiu

{"title":"Activation of atrial natriuretic peptide receptor A depresses hypothalamic PVN CRFergic neuronal excitability by inhibiting IH in vitro in mice","authors":"Yong-Rui Piao , Yang Liu , Chun-Ping Chu , Yu-Zi Li , De-Lai Qiu","doi":"10.1016/j.neulet.2025.138202","DOIUrl":"10.1016/j.neulet.2025.138202","url":null,"abstract":"<div><div>Atrial natriuretic peptide (ANP) and its receptor are abundantly distributed in hypothalamic paraventricular nucleus (PVN), and contribute to regulation of corticotropin releasing factor (CRF) release in the brain. However, the mechanism by which ANP regulates the activity of PVN CRFergic neurons remains unclear. Here, we investigated the mechanism by which ANP modulates the neuronal activity related to PVN CRF-mRNA expression <em>in vitro</em> in mice using whole-cell patch-clamp recording, single-cell RT-PCR, immunofluorescence, and pharmacological methods. Our data demonstrated that ANP significantly reduced the membrane excitability of PVN neurons expressing CRF-mRNA. This effect was abolished by an antagonist of ANP receptor A (NPR − A), namely A71915. Furthermore, ANP significantly suppressed the hyperpolarization − activated cationic currents (I<sub>h</sub>) of neurons expressing CRF-mRNA. Blockade of either I<sub>h</sub> or NPR − A completely prevented this inhibitory effect. Immunohistochemistry results indicated that NPR − A immunoreactivity was present in PVN CRF-positive neurons. These findings suggest that ANP decreases the excitability of PVN CRFergic neurons via NPR − A − mediated inhibition of I<sub>h</sub> channel activity <em>in vitro</em> in mice.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"853 ","pages":"Article 138202"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circuit-specific neural perturbations and recovery in methamphetamine addiction in a mouse model

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-16 DOI: 10.1016/j.neulet.2025.138201

Pongpanot Wongveerakul , Dania Cheaha , Ekkasit Kumarnsit , Nifareeda Samerphob

{"title":"Circuit-specific neural perturbations and recovery in methamphetamine addiction in a mouse model","authors":"Pongpanot Wongveerakul , Dania Cheaha , Ekkasit Kumarnsit , Nifareeda Samerphob","doi":"10.1016/j.neulet.2025.138201","DOIUrl":"10.1016/j.neulet.2025.138201","url":null,"abstract":"<div><div>Drug addiction is characterized by profound brain adaptations, including altered neural circuit dynamics in reward-related regions, which reinforce compulsive drug-seeking behavior. This study investigated the effects of 5 mg/kg methamphetamine (METH) administration on brain activity measured by local field potentials (LFPs) in the nucleus accumbens (NAc) and dorsal hippocampus (dHP) of C57BL/6 mice. The study further examined the sensitivity of these brain regions during an abstinent period on day 8 and following a low-dose METH challenge. METH administration reduced theta power activity and enhanced gamma activity in the NAc, but decreased alpha2 power with specific high gamma increases in the dHP during conditioning and challenge phases. The sleep analysis revealed a reduction in NREM during the conditioning and challenge phases, however, these parameters returned to normal after 8 days abstinence from METH. These findings suggest that repeated METH administration induces neural sensitization and alters sleep architecture. However, the minimization of adverse neural changes, particularly in sleep regulation, highlights potential avenues for therapeutic applications in managing addiction and promoting recovery.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"853 ","pages":"Article 138201"},"PeriodicalIF":2.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studying the effect of Chlamydia trachomatis, Helicobacter pylori, and Varicella zoster microorganisms in stimulating the expression of cytokines TNFα, IFNɤ, TGFβ, IL-10 in Alzheimer and non-Alzheimer patients

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-15 DOI: 10.1016/j.neulet.2025.138192

Sima Taheri , Mansoureh Paknejadi , Davoud Esmaeili , Atousa Ferdousi , Mohammad Hassan Shahhosseiny

{"title":"Studying the effect of Chlamydia trachomatis, Helicobacter pylori, and Varicella zoster microorganisms in stimulating the expression of cytokines TNFα, IFNɤ, TGFβ, IL-10 in Alzheimer and non-Alzheimer patients","authors":"Sima Taheri , Mansoureh Paknejadi , Davoud Esmaeili , Atousa Ferdousi , Mohammad Hassan Shahhosseiny","doi":"10.1016/j.neulet.2025.138192","DOIUrl":"10.1016/j.neulet.2025.138192","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to use the real-time RT-PCR method to detect the gene expression cytokines IL-10, TNFα, IFN-γ, and TGF-β in the serum of Alzheimer’s patients.</div></div><div><h3>Methods</h3><div>This study was conducted on 100 serum samples of Alzheimer’s patients. DNA extraction was performed on the samples with the Cinnaclone kit and PCR techniques were used to detect the presence of <em>Helicobacter pylori</em>, <em>Chlamydia trachomatis</em>, and <em>Varicella zoster virus</em>. Real-time RT-PCR was performed to measure the expression of TNFα, IFNɤ, TGFβ, and IL-10 genes with a Smobio kit.</div></div><div><h3>Results</h3><div><strong>T</strong>he relative changes in the expression of TNFα, IFNɤ, TGFβ, and IL-10 genes were observed in Alzheimer’s patients compared to the control samples without microorganisms, and a significant increase was observed (P < 0.05).</div></div><div><h3>Conclusion</h3><div>This study showed that the cytokines TNFα, IFNɤ, TGFβ, and IL-10, have an increase in Alzheimer’s patients(P < 0.05). Therefore, the presence of the microorganisms accompanied by the rise and inducing the expression of cytokines compared to the groups without the mentioned microorganisms causes a significant increase in the production of cytokines effective in the occurrence or exacerbation of Alzheimer’s disease.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"852 ","pages":"Article 138192"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galbanic acid delays the development of seizures by modulating the expression of TNFα, IL1β, and TLR4 genes and reducing hippocampal nitrite levels and may be useful in the treatment of epilepsy 高良姜酸通过调节 TNFα、IL1β 和 TLR4 基因的表达，降低海马亚硝酸盐水平，从而延缓癫痫发作的发展，可能有助于治疗癫痫。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-15 DOI: 10.1016/j.neulet.2025.138200

Najmeh Asgharzadeh , Saeid Ghavamnia , Hossein Amini-Khoei , Zahra Lorigooini , Marzieh Mardani , Elham Bijad , Diana shahrani , Mohamad shahrani , Mehrdad Shahrani korrani

{"title":"Galbanic acid delays the development of seizures by modulating the expression of TNFα, IL1β, and TLR4 genes and reducing hippocampal nitrite levels and may be useful in the treatment of epilepsy","authors":"Najmeh Asgharzadeh , Saeid Ghavamnia , Hossein Amini-Khoei , Zahra Lorigooini , Marzieh Mardani , Elham Bijad , Diana shahrani , Mohamad shahrani , Mehrdad Shahrani korrani","doi":"10.1016/j.neulet.2025.138200","DOIUrl":"10.1016/j.neulet.2025.138200","url":null,"abstract":"<div><h3>Introduction and Objective</h3><div>Epilepsy is one of the most common chronic brain disorders worldwide. This study aimed to determine the effect of Galbanic acid on seizures.</div></div><div><h3>Materials and Methods</h3><div>In this study, 40 NMRI mice were used and divided into 4 groups. The control group received 10 mg/kg normal saline. Three groups received Galbanic acid at doses of 10, 20, and 40 mg/kg. The positive control group received diazepam with a dose of 10 mg/kg. All injections were made intraperitoneally for one week. Seizures were induced in all mice by injection of 90 mg/kg Pentylenetetrazole (PTZ). Then the latency of seizure onset in each of the experimental groups was recorded. After anesthesia with Ketamine 100 mg/kg and Xylazine 10 mg/kg, blood samples were taken and after decapitation, the hippocampus was isolated and the expression of αTNF-, IL1β, and TLR4 genes in this region was measured.</div></div><div><h3>Results</h3><div>Galbanic acid increased the delay in seizure onset, decreased brain and serum nitrite levels, and decreased the expression of αTNF-, IL1β, and TLR4 genes in brain tissue compared to the control group (P < 0.01, p < 0.001).</div></div><div><h3>Conclusion</h3><div>Galbanic acid reduced nitrite and the expression of αTNF-, IL1β, and TLR4, leading to a decrease in neuroinflammation and, as a result, a delay in seizure onset in mice.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"853 ","pages":"Article 138200"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to “Abnormal innervation patterns in the anorectum of ETU-induced fetal rats with anorectal malformations” [Neurosci. Lett. 495 (2011) 88–92]

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-15 DOI: 10.1016/j.neulet.2025.138196

Weilin Wang , Huimin Jia , Hailan Zhang , Qingjiang Chen , Tao Zhang , Yuzuo Bai , Zhengwei Yuan

引用次数: 0

Linking oxytocin to nicotine dependence: An experimental study of the brain, behavior, and gut microbiota in rats

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-15 DOI: 10.1016/j.neulet.2025.138198

Ruiyan Wang , Guanglin Liu , Huan Chen , Hongwei Hou , Qingyuan Hu

{"title":"Linking oxytocin to nicotine dependence: An experimental study of the brain, behavior, and gut microbiota in rats","authors":"Ruiyan Wang , Guanglin Liu , Huan Chen , Hongwei Hou , Qingyuan Hu","doi":"10.1016/j.neulet.2025.138198","DOIUrl":"10.1016/j.neulet.2025.138198","url":null,"abstract":"<div><div>Oxytocin (OXT) is a hypothalamic neuropeptide, and numerous studies have indicated that exposure to addictive substances, such as opioids, cocaine, etc., can result in decreased function of the OXT system. The study also found that OXT can reduce addictive behavior for certain drugs, including methamphetamine, alcohol, and cocaine, suggesting a close relationship between the OXT system and drug abuse. Although nicotine is the main addictive substance in tobacco, its interaction with the OXT system is unknown and requires further study. Therefore, OXT levels in plasma and brain regions associated with addiction were measured by enzyme-linked immunosorbent assay (ELISA) using chronic nicotine administration via a slow-release pump. In addition, the effects of OXT injection on nicotine self-administration behavior, motor activity, and intestinal microbiota in rats were examined by nicotine self-administration experiment, open field experiment, and 16S sequencing experiment. By depleting gut microbiota with oral antibiotics, this study aims to investigate whether gut microbiota mediates oxytocin effect on the nicotine self-administration behavior in rats. This study shows that chronic nicotine administration can reduce OXT levels in plasma and brain regions such as the paraventricular nucleus (PVN), ventral tegmental area (VTA), and caudate putamen (CPU). OXT at a dose of 1.0 mg/kg significantly reduced the number of nicotine infusions and the abundance of Lactobacillus in rats. Notably, our findings indicate that other mechanisms besides gut microbes are involved in the effect of peripheral OXT administration on the inhibition of nicotine self-administration.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"852 ","pages":"Article 138198"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0