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Chemometric characterization and classification of new wheat genotypes 小麦新基因型的化学计量学特征及其分类
Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1293
F. Kraic, J. Mocák
{"title":"Chemometric characterization and classification of new wheat genotypes","authors":"F. Kraic, J. Mocák","doi":"10.36547/nbc.1293","DOIUrl":"https://doi.org/10.36547/nbc.1293","url":null,"abstract":"The final goal of this work is development of new genotypes of wheat with better properties compared to the standard set. Prediction of optimal descriptors and properties related to the production characteristics is performed using several statistical and chemometrical tools, like correlation analysis, principal component analysis, cluster analysis and linear discriminant analysis. Optimisation of wheat genotypes is directed towards high food quality.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45077058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Zinc uptake and distribution in ivy (Hedera helix L.) leaves 常春藤叶片对锌的吸收与分布
Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1289
J. Marešová, M. Horník, M. Pipíška, J. Augustín
{"title":"Zinc uptake and distribution in ivy (Hedera helix L.) leaves","authors":"J. Marešová, M. Horník, M. Pipíška, J. Augustín","doi":"10.36547/nbc.1289","DOIUrl":"https://doi.org/10.36547/nbc.1289","url":null,"abstract":"Detached leaves of ivy (Hedera helix L.) were used as a model for the study of zinc uptake and transport in vascular plants. By the uptake via the surface of fully immersed leaves in 25 % Hoagland nutrient media (HM) spiked with 65ZnCl2 (50 μmol/dm3 ZnCl2), concentration in leaves 4.98 μg Zn/g (dry wt.), i. e. 2.6 μg Zn/dm2 leaf area after 7d exposition were obtained. By the uptake via immersed stalks of not immersed (transpiring) leaves concentrations up to 370 μg Zn/g (dry wt.) were obtained. When Zn enters into detached leaves via the surface of immersed leaf blades, zinc is uniformly distributed in leaf blades and leaf stalks. When zinc enters detached leaves via immersed stalks of non-immersed transpiring leaves, only small part of zinc is transported to leaf blades and the prevailing part remains in leaf stalks. Stalks act as a trap, able to prevent other leaf tissues against inhibitory effects of high Zn concentrations. Mineral nutrient salts in solutions mobilize Zn trapped in leaf stalks and facilitate Zn transport by transpiration stream to leaf blades, what means that Zn in stalks is bound in ion-exchageable forms.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43679130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of IUPAC limit of detection and ISO minimum detectable value - electrochemical determination of lead IUPAC检测限和ISO最小检测值的评价——铅的电化学测定
Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1291
J. Mocák, I. Janiga, Estera Rábarová
{"title":"Evaluation of IUPAC limit of detection and ISO minimum detectable value - electrochemical determination of lead","authors":"J. Mocák, I. Janiga, Estera Rábarová","doi":"10.36547/nbc.1291","DOIUrl":"https://doi.org/10.36547/nbc.1291","url":null,"abstract":"The way of calculating the limit of detection recommended by IUPAC is compared to the minimum detectable value used by ISO as one of the most important performance characteristics of a measurement process. In this work, theoretical analysis of both characteristics is given together with directions for their practical use. Calculations are exemplified using electrochemical trace analysis of lead in surface water.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49467611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Multiplex PCR method for detection of variability in genes encoding the alpha proteins of Streptococcus agalactiae 多重PCR检测无乳链球菌α蛋白基因变异的方法
Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1288
B. Vidová, M. Chotár, J. Timko, A. Godány
{"title":"Multiplex PCR method for detection of variability in genes encoding the alpha proteins of Streptococcus agalactiae","authors":"B. Vidová, M. Chotár, J. Timko, A. Godány","doi":"10.36547/nbc.1288","DOIUrl":"https://doi.org/10.36547/nbc.1288","url":null,"abstract":"The majority of Streptococcus agalactiae strains express one or more surface-anchored proteins that vary by strain. These proteins, which are characteristic for S. agalactiae, and are able to induce protective antibodies, include the alpha C, Rib proteins, alpha-like protein 2, and alpha-like protein 3. In this study was developed multiplex PCR method for detection of genes encoding these proteins, and its occurrence within a various S. agalactiae isolates of bovine origin. Also are reported two new genes from bovine isolates of S. agalactiae amplified by PCR, encoding other putative members of the family, alphalike protein 6, and alpha-like protein 7. They contain an overall genetic organization highly similar to that of the alpha C and Rib proteins.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44099989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Sorption of cationic dyes from aqueous solutions by moss Rhytidiadelphus squarrosus: Kinetics and equilibrium studies 苔藓Rhytidiadelphus squarosus从水溶液中吸附阳离子染料的动力学和平衡研究
Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1287
Lucia Remenárová, M. Horník, J. Augustín, J. Herdu
{"title":"Sorption of cationic dyes from aqueous solutions by moss Rhytidiadelphus squarrosus: Kinetics and equilibrium studies","authors":"Lucia Remenárová, M. Horník, J. Augustín, J. Herdu","doi":"10.36547/nbc.1287","DOIUrl":"https://doi.org/10.36547/nbc.1287","url":null,"abstract":"With the aim to investigate sorption properties of natural sorbent prepared from moss Rhytidiadelphus squarrosus we elucidated biosorption of cationic dyes Malachite green (BG4), Auramine O (BY2) and Thioflavine T (BY1) from aqueous solutions. The removal of dyes by moss biosorbent was found to be rapid at an initial stage and the equilibrium was reached within 1-2 hours. The pseudo-n-order kinetic model was successfully applied to the kinetic data and the order of adsorption reaction was calculated in the range from 1.7 to 2.6. The value of rate constant kn' ranged from 0.001 to 0.039 [min-1]/[μmol/g]1-n. The equilibrium data were fitted to the adsorption isotherms. The Freundlich isotherm was found to represent the measured sorption data of BG4, BY1 and BY2 well. The maximum sorption capacities of moss biomass from single dye solutions calculated by Langmuir equation were 354 μmol/g for BG4, 310 μmol/g for BY1 and 382 μmol/g for BY2. These results showed that the prepared biomass presents low-cost, natural and easy available sorbent which may be potentially used for removal of dyes from environment and also may be an alternative to more costly materials such as activated carbon.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41695748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Relationship between administration of statins and blood serum levels of selected biochemical parameters 他汀类药物给药与血清某些生化参数水平的关系
Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1290
J. Mocák, J. Balla, Gabriela Gromanová, K. Boronová
{"title":"Relationship between administration of statins and blood serum levels of selected biochemical parameters","authors":"J. Mocák, J. Balla, Gabriela Gromanová, K. Boronová","doi":"10.36547/nbc.1290","DOIUrl":"https://doi.org/10.36547/nbc.1290","url":null,"abstract":"Results of biochemical tests of 172 patient data (among them 84 men data and 88 women data, resp.) before and after administration of statins were studied. All monitored patients are characterized by lipoprotein metabolism failure or other kind of lipidaemia. The calculations were performed using several chemometrical methods pursuing visualization of most important biochemical parameters and classification of the patient blood samples by means of up-to-date software packages. The dependences of the content of most common biochemical parameters upon the treatment of patients by statins were elucidated in detail.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49530571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Linear and non-linear regression analysis for the biosorption kinetics of methylene blue 亚甲基蓝生物吸附动力学的线性和非线性回归分析
Nova Biotechnologica et Chimica Pub Date : 2021-11-22 DOI: 10.36547/nbc.1278
J. Augustín
{"title":"Linear and non-linear regression analysis for the biosorption kinetics of methylene blue","authors":"J. Augustín","doi":"10.36547/nbc.1278","DOIUrl":"https://doi.org/10.36547/nbc.1278","url":null,"abstract":"The nonviable biomass of Rhizopus sp. R-18, Penicillium candidum and Penicillium chrysogenum was studied for biosorption of methylene blue (MB). The sorption of MB was studied be performing batch kinetic experiments. Kinetic measurements showed that sorption of MB reached equilibrium in 4 hours. The batch sorption models, based on a pseudo-first, pseudo-second and pseudo-nth order were applied to predict the rate constant of sorption and the equilibrium capacity. The linear and nonlinear least-square methods were used to obtain the kinetic parameters. The best-fit model was identified using statistic analysis. The results showed that both linear and nonlinear form of pseudo- second order expression could be used to fit the experimental data but nonlinear method may be a better way to obtain the desired parameters. As well the pseudo n-th order kinetic model was successfully applied to the kinetic data. The order (n) of adsorption reaction was found for all employed biosorbents: for Rhizopus sp. R-18 it had value 3.1, P. candidum 3.0 and P. chrysogenum 3.8.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49087695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
High-performance liquid chromatographic detection of selected phenolic acidsin wine 葡萄酒中酚酸的高效液相色谱检测
Nova Biotechnologica et Chimica Pub Date : 2021-11-22 DOI: 10.36547/nbc.1157
Darinka Brodnjak Vončina, Maša Islamčivic Razboršek, M. Simonič
{"title":"High-performance liquid chromatographic detection of selected phenolic acidsin wine","authors":"Darinka Brodnjak Vončina, Maša Islamčivic Razboršek, M. Simonič","doi":"10.36547/nbc.1157","DOIUrl":"https://doi.org/10.36547/nbc.1157","url":null,"abstract":"The aim of this study was to develop a method for identification and quantification of phenolic acids in different wine samples. The simple reversed-phase HPLC-UV method for simultaneous determination of p-coumaric and ferulic acid was developed. The method was validated and working range, linearity, repeatability, accuracy, limit of quantitation LOQ and limit of detection LOD were determined. The linearity of the method was tested in concentration ranges 0.1-1 mg L-1 and 1-10 mg L-1. The correlation coefficients (r2) were greater than 0.996 and quality coefficients (QC) ≤ 5%. Detection limit for both compounds was 0.01 mg L-1. The method is precise (RSD","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49409816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Positive and negative aspects of suplhate-reducing bacteria in environment and industry 硫酸盐还原菌在环境和工业中的积极和消极方面
Nova Biotechnologica et Chimica Pub Date : 2021-11-22 DOI: 10.36547/nbc.1271
A. Luptáková, E. Mačingová, V. Harbuláková
{"title":"Positive and negative aspects of suplhate-reducing bacteria in environment and industry","authors":"A. Luptáková, E. Mačingová, V. Harbuláková","doi":"10.36547/nbc.1271","DOIUrl":"https://doi.org/10.36547/nbc.1271","url":null,"abstract":"The submitted work is oriented on the study of two aspects of the sulphate-reducing bacteria metabolism: the metals bioprecipitation and the concrete biodeterioration. The bioprecipitation of metals with the bacterially produced hydrogen sulphide by sulphate-reducing bacteria (SRB) represents the positive effect of the SRB existence in the environment. It allows the industrial exploitation in the area of the removal metals from industrial wastewaters. Referred method involves principal stages such as: hydrogen sulphide bacterial production, metals precipitation by biologically produced hydrogen sulphide, metal sulphides separation, setting pH of the filtrate from previous steps by 1M NaOH and metal hydroxides separation. The basis of the first stage i.e. the hydrogen sulphide \u0000bacterial production is the cultivation of SRB. In the laboratory conditions the sodium lactate is the energetic substrate for the growth of bacteria. Its price is not economic for the application in the practice and is needed investigate the alternative substitutes. Therefore was studied the cultivation of sulphate-reducing bacteria to using the selected energetic substrates such as: calcium lactate, glycerol and whey. Experimental studies confirm that all chosen substrates are suitable alternative substrates of sodium lactate for the bacterial sulphate-reduction. In the regard to the efficiency of bacterial sulphate reduction the calcium lactate is the best. The biodeterioration of the concrete presents the negative effect of the SRB existence in the environment. The research was oriented on the simulation of the biodeterioration of concrete samples under the simultaneous influence of the sulphur-oxidising bacteria genera Acidithiobacillus thiooxidans and sulphatereducing bacteria genera Desulfovibrio in the environs of the waste water, the acid mine drainage, the nutrient medium and the distilled water. The observation of the surface structure changes of concrete samples confirms the highest biodeterioration influences in the case of the acid mine drainage application.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46381499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Applications substituted 2-aminothiophenes in drug design 取代2-氨基噻吩在药物设计中的应用
Nova Biotechnologica et Chimica Pub Date : 2021-11-22 DOI: 10.36547/nbc.1274
Zita Puterová, D. Végh
{"title":"Applications substituted 2-aminothiophenes in drug design","authors":"Zita Puterová, D. Végh","doi":"10.36547/nbc.1274","DOIUrl":"https://doi.org/10.36547/nbc.1274","url":null,"abstract":"Highly substituted thiophene derivatives are important heterocycles found in numerous biologically active compounds. Title compounds are attractive derivatives because their applications in pharmaceuticals, agriculture and pesticides. They exhibit antimicrobial activity against various Gram(+) and Gram(-) bacteria and fungi. Many of these molecules act as allosteric enhancers of A1-adenosine receptor, glucagon antagonists as well as antioxidant and anti-inflammatory agents.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49108097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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