多重PCR检测无乳链球菌α蛋白基因变异的方法

Q4 Agricultural and Biological Sciences

Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI:10.36547/nbc.1288

B. Vidová, M. Chotár, J. Timko, A. Godány

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引用次数: 1

摘要

大多数无乳链球菌菌株表达一种或多种表面锚定蛋白，这些蛋白因菌株而异。这些蛋白质是无乳链球菌的特征，并且能够诱导保护性抗体，包括αC、肋蛋白、α样蛋白2和α样蛋白3。在这项研究中，开发了一种多重PCR方法来检测编码这些蛋白质的基因，以及它在各种牛源无乳双歧杆菌分离株中的出现。此外，还报道了两个来自无乳链球菌牛分离株的新基因，通过PCR扩增，编码该家族的其他假定成员，α样蛋白6和α样蛋白7。它们包含一个与阿尔法C和肋骨蛋白高度相似的整体遗传组织。

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Multiplex PCR method for detection of variability in genes encoding the alpha proteins of Streptococcus agalactiae

The majority of Streptococcus agalactiae strains express one or more surface-anchored proteins that vary by strain. These proteins, which are characteristic for S. agalactiae, and are able to induce protective antibodies, include the alpha C, Rib proteins, alpha-like protein 2, and alpha-like protein 3. In this study was developed multiplex PCR method for detection of genes encoding these proteins, and its occurrence within a various S. agalactiae isolates of bovine origin. Also are reported two new genes from bovine isolates of S. agalactiae amplified by PCR, encoding other putative members of the family, alphalike protein 6, and alpha-like protein 7. They contain an overall genetic organization highly similar to that of the alpha C and Rib proteins.

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来源期刊

Nova Biotechnologica et Chimica Agricultural and Biological Sciences-Food Science

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

24 weeks