Nova Biotechnologica et Chimica最新文献_第7页

DNA damage by oxidized fatty acids detected by DNA/SPE biosensor DNA/SPE生物传感器检测氧化脂肪酸对DNA的损伤

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1307

Ľudmila Sirotová, Marcela Matulová

引用次数: 0

Computer-aided diagnosis of lung malignity using multidimensional analysis of tumour marker data 基于肿瘤标志物数据多维分析的肺恶性肿瘤计算机辅助诊断

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1309

V. Mrázová, J. Mocák, E. Varmusová, Denisa Kavková

引用次数: 0

Phage display – a tool for detection and prevention against pathogens. A review 噬菌体展示——一种检测和预防病原体的工具。综述

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1304

B. Vidová, A. Godány

引用次数: 0

Rapid detection methods of microbial pathogens in foods – a short survey 食品中微生物病原体的快速检测方法——一项简短的调查

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1303

B. Vidová, A. Godány

引用次数: 0

Influence of nutrients in soil and vine leaves and meteorological factors upon vine crop and must quality 土壤和葡萄叶片养分及气象因子对葡萄产量和果实品质的影响

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1310

F. Kraic, J. Mocák, Miroslav Argay

引用次数: 0

Bioaccumulation of 137Cs and 60Co in freshwater plants 137Cs和60Co在淡水植物中的生物累积

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1308

M. Horník, M. Pipíška, J. Augustín

{"title":"Bioaccumulation of 137Cs and 60Co in freshwater plants","authors":"M. Horník, M. Pipíška, J. Augustín","doi":"10.36547/nbc.1308","DOIUrl":"https://doi.org/10.36547/nbc.1308","url":null,"abstract":"Contamination of the aquatic environment by the heavy metals and radionuclides has become a serious concern in the world. In our study, gamma-spectrometry of freshwater plants Bacopa monnieri and Egeria densa growing in cultivation media spiked with 137CsCl and 60CoCl2 was used for quantitative determination of bioaccumulation kinetic and distribution Cs+ and Co2+ ions in plant tissues. We found, that bioaccumulation of Cs and Co by fully immersed B. monnieri in Hoagland media (HM) was dependent on ion concentration in medium. Approx. 5-times lower Cs uptake 2.9 nmol/g (d.w.) was obtained in plants cultivated in 20% HM than from deionized water. The maximal Co uptake was 4-times higher than cesium uptake at the same conditions. Both Cs and Co were localized mainly in roots. The highest immobilization from roots to shoots was found in the case of Co uptake from deionized water with concentration ratio [Co]leaves : [Co]stem : [Co]root = 1.00 : 5.33 : 56.8. Cesium uptake by submerged plant E. densa was also strongly dependent on nutrients concentration in medium. However, in the case of cobalt uptake this dependence was less pronounced. Nutrients concentration also had a significant influence on distribution of Cs between stems and leaves of E. densa. Cesium was localized in leaves, however with increasing of nutrients concentration in cultivation media Cs was localized for account of stem. On the other hand, cobalt was immobilized mainly in leaves in whole range of nutrients concentration. Obtained data can serve as a models for understanding of phytoaccumulation of radionuclides from open water ponds and water channels in the vicinity of nuclear power plants and monovalent and bivalent metals from industrial sources of contamination.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44632847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Formation of Streptomyces protoplasts during cultivation in liquid media with lytic enzyme 链霉菌原生质体在溶酶液体培养基中培养过程中的形成

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI: 10.36547/nbc.1305

Z. Brnáková, A. Rusnáková, A. Godány, J. Herdu

{"title":"Formation of Streptomyces protoplasts during cultivation in liquid media with lytic enzyme","authors":"Z. Brnáková, A. Rusnáková, A. Godány, J. Herdu","doi":"10.36547/nbc.1305","DOIUrl":"https://doi.org/10.36547/nbc.1305","url":null,"abstract":"Many streptomycetes strains are hardly or not at all transformable via protoplasts, or there is a problem with the regeneration of protoplasts. We found that protoplasts are formed directly in cultivation media under submerged conditions in the presence of lytic enzyme. Actinophage μ1/6 endolysin and lysozyme were used in this study. Streptomyces strains were cultivated in several media with glycine and lytic enzyme for 24 and 48h. The highest amounts of protoplasts (about 3 x 107 cfu/ml of cultivation medium) together with the highest regeneration (95%) and transformation frequency (about 2 x 106 – 107 cfu/μg DNA) were obtained reproducibly in YEME medium with high sucrose content. S. aureofaciens B96, as hardly transformable strain because of difficulties with protoplast preparation and their further regeneration, was used in this study. The same procedure was applied to S. lividans 66 TK24 and S. coelicolor A3(2), streptomycetes model strains, to confirm the general use of this method. Moreover, such cultivation process was appropriate for additional quick isolation of either chromosomal as well as plasmid DNA that could be further used in recombinant DNA techniques.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69625296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequences analysis of chloroplast psbA-trnH region in Citrus L. (Rutaceae) species from the Aegean region of Turkey 土耳其爱琴海地区柑橘属植物叶绿体psbA-trnH区序列分析

Nova Biotechnologica et Chimica Pub Date : 2021-11-30 DOI: 10.36547/nbc.877

E. Sevindik, Gaye Zeynep Canbolat, İlayda İrem Moral, M. Sujka

{"title":"Sequences analysis of chloroplast psbA-trnH region in Citrus L. (Rutaceae) species from the Aegean region of Turkey","authors":"E. Sevindik, Gaye Zeynep Canbolat, İlayda İrem Moral, M. Sujka","doi":"10.36547/nbc.877","DOIUrl":"https://doi.org/10.36547/nbc.877","url":null,"abstract":"In this study, sequences analysis of some Citrus species distributed in Turkey's Aegean region was based on the cpDNA psbA-trnH region. The sequences for psbA-trnH regions of the outgroups were retrieved from NCBI GenBank. Genomic DNA was isolated from healthy and green leaves. Total genomic DNA was extracted using GeneMark DNA isolation Plant Kit. The psbA-trnH region was amplified using primers psbA and trnH. DNA sequences were edited using the Sequencher 5.4.6. Sequencing data were analyzed using MEGA 6.0 software. Maximum likelihood (ML) tree was created to determine the relationships between Citrus taxa. cpDNA psbA-trnH sequences ranged between 426 and 470 nucleotides. Maximum likelihood phylogenetic tree is composed of two clades. The divergence values differed between 0.000 and 0.012. According to the results of the study, the separation of Citrus species in phylogenetic tree obtained with psbA-trnH sequence data was realized. However, it has been found that cpDNA psbA-trnH sequence populations of species belong together. In addition, the phylogenetic relationship between the sequence data of some species belonging to the Rutaceae family taken from NCBI and Citrus species was revealed.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42932143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Amylolytic enzymes - focus on the alpha-amylases from Archae and plants 淀粉酶-专注于来自古菌和植物的α淀粉酶

Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1284

Š. Janeček

{"title":"Amylolytic enzymes - focus on the alpha-amylases from Archae and plants","authors":"Š. Janeček","doi":"10.36547/nbc.1284","DOIUrl":"https://doi.org/10.36547/nbc.1284","url":null,"abstract":"Amylolytic enzymes represent a group of starch hydrolases and related enzymes that are active towards the α-glycosidic bonds in starch and related poly- and oligosaccharides. The three best known amylolytic enzymes are α-amylase, β-amylase and glucoamylase that, however, differ from each other by their amino acid sequences, three-dimensional structures, reaction mechanisms and catalytic machineries. In the sequence-based classification of all glycoside hydrolases (GHs) they have therefore been classified into the three independent families: GH13 (α-amylases), GH14 (β-amylases) and GH15 (glucoamylases). Some amylolytic enzymes have been placed to the families GH31 and GH57. The family GH13 together with the families GH70 and GH77 constitutes the clan GH-H, well-known as the α-amylase family. It contains more than 6,000 sequences and covers 30 various enzyme specificities sharing the conserved sequence regions, catalytic TIM-barrel fold, retaining reaction mechanism and catalytic triad. Among the GH13 α-amylases, those produced by plants and archaebacteria exhibit common sequence similarities that distinguish them from the α-amylases of the remaining taxonomic sources. Despite the close evolutionary relatedness between the plant and archaeal α-amylases, there are also specific differences that discriminate them from each other. These specific differences could be used in an effort to reveal the sequence-structural features responsible for the high thermostability of the α-amylases from Archaea.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46619897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Genetic improvement of wheat - A review 小麦遗传改良研究进展

Nova Biotechnologica et Chimica Pub Date : 2021-11-29 DOI: 10.36547/nbc.1285

E. Gregová

引用次数: 14