DNA/SPE生物传感器检测氧化脂肪酸对DNA的损伤

Q4 Agricultural and Biological Sciences

Nova Biotechnologica et Chimica Pub Date : 2021-12-04 DOI:10.36547/nbc.1307

Ľudmila Sirotová, Marcela Matulová

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引用次数: 0

摘要

电化学DNA/丝网印刷电极生物传感器(DNA/SPE生物传感器)用于检测DNA与脂肪酸氧化产物之间反应所形成的DNA变化。DNA与亚油酸和油酸氧化过程中产生的混合物的相互作用表现出DNA损伤，这取决于所测试的脂肪酸以及脂肪酸氧化后确定的氢过氧化物和硫代巴比妥酸反应物质(TBARS)的存在。与TBARS含量高的氧化亚油酸相互作用时，DNA损伤程度更大。研究结果提示了DNA/SPE生物传感器在检测DNA与脂肪酸氧化产物相互作用中的应用前景。

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DNA damage by oxidized fatty acids detected by DNA/SPE biosensor

Electrochemical DNA/screen-printed electrode biosensor (DNA/SPE biosensor) was tested for the detection of alterations in DNA formed as a consequence of the reaction between DNA and oxidative products of fatty acids. Interaction of DNA with a mixture of products generated during the oxidation of linoleic and oleic acids manifested DNA damage depending on a tested fatty acid and the presence of hydroperoxides and thiobarbituric acid reactive substances (TBARS) determined after the oxidation of fatty acids. A bigger extent of the DNA damage was registered in the case of the interaction with oxidized linoleic acid with the high content of TBARS. The results achieved suggest the possible application of DNA/SPE biosensor in the detection of an interaction between DNA and products of fatty acid oxidation.

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来源期刊

Nova Biotechnologica et Chimica Agricultural and Biological Sciences-Food Science

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

24 weeks