Neurourology and Urodynamics最新文献

{"title":"Urodynamics in the Evaluation and Management of Bothersome Lower Urinary Tract Symptoms in Men With a Prostate.","authors":"Joshua A Cohn","doi":"10.1002/nau.70076","DOIUrl":"https://doi.org/10.1002/nau.70076","url":null,"abstract":"<p><strong>Introduction: </strong>Despite their utility, urodynamics (UDS) are associated with medical risks and patient discomfort. Therefore, it is crucial to select patients for UDS where the results are likely to change clinical decision-making or solidify diagnostic confidence. This narrative review aims to assess the indications, benefits and limitations of UDS in men with a prostate who have refractory lower urinary tract symptoms.</p><p><strong>Methods: </strong>A comprehensive review of the literature was conducted, focusing on studies evaluating the utility, patient perspectives, and clinical guidelines related to the use of UDS.</p><p><strong>Results: </strong>UDS are beneficial in specific clinical scenarios, such as in patients with neurogenic lower urinary tract dysfunction (NLUTD) or when the likelihood of successful intervention is uncertain. For men with suspected BPH, routine UDS before surgical intervention may not be necessary unless there are comorbid conditions such as frailty or a history of radiation that exacerbate risk of surgery or in the presence of significant diagnostic uncertainty, such as in men with suspected neurogenic lower urinary tract dysfunction.</p><p><strong>Conclusions: </strong>UDS should be selectively utilized in clinical practice to maximize their diagnostic and therapeutic benefits while minimizing patient discomfort and risks. In men with suspected BPH, UDS should be considered in cases with diagnostic uncertainty or in the presence of significant comorbidities. Further research is needed to refine the indications for UDS and to develop noninvasive alternatives that can provide similar diagnostic insights.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urodynamics for Female Stress Urinary Incontinence: When and Why.","authors":"Courtney K Pfeuti, Mikolaj Przydacz, Brian J Linder","doi":"10.1002/nau.70073","DOIUrl":"https://doi.org/10.1002/nau.70073","url":null,"abstract":"<p><strong>Aims: </strong>To discuss the use of urodynamic testing for female stress urinary incontinence and provide a narrative review of the current evidence regarding the utility of urodynamics across the spectrum of clinical presentations.</p><p><strong>Methods: </strong>A nonsystemic, extensive literature review via PubMed was performed on the use of urodynamics for female stress urinary incontinence.</p><p><strong>Results: </strong>Findings from several large multicenter trials and subsequent secondary analyses have significantly influenced treatment patterns away from the routine use of urodynamics in female patients with uncomplicated stress urinary incontinence. Much of the current literature focuses on selected patients with uncomplicated stress urinary incontinence that are subsequently treated with midurethral sling placement. These studies comprise the foundational evidence supporting multiple national and international guidelines on the topic. In uncomplicated patients, these guidelines do not support the routine use of urodynamics. However, in more complicated presentations, urodynamic studies are more often valuable for diagnosis and management.</p><p><strong>Conclusions: </strong>Routine use of urodynamic testing is not currently recommended in guidelines for evaluating uncomplicated female stress urinary incontinence. It is important to recognize that much of the evidence supporting these recommendations is derived from a select population of uncomplicated patients, which may limit generalizability to more complex cases. In such cases, urodynamic testing may be more useful to guide diagnosis and management, though further research is needed to identify which findings have the greatest impact on clinical outcomes.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Holm and Cordoba Urinary Tract Infection Score: Translation, Linguistic and Content Validation of the German Version of a Patient-Reported Outcome Measure to Assess Symptoms, Bothersomeness and Impact of Uncomplicated Urinary Tract Infections in Women.","authors":"Katharina Piontek, Sophie Nestler, Ebru Özkan, Anne Holm, John Brandt Brodersen, Christian Apfelbacher","doi":"10.1002/nau.70066","DOIUrl":"https://doi.org/10.1002/nau.70066","url":null,"abstract":"<p><strong>Background: </strong>The Danish Holm and Cordoba Urinary Tract Infection Score (HCUTI) assessing symptom severity, bothersomeness and impact of uncomplicated urinary tract infections (uUTIs) on daily activities in women is a promising patient-reported outcome measure (PROM) for use in future research. For potential application in Germany, the present study aimed (i) to perform translation and linguistic validation of the HCUTI, and (ii) to assess content validity of the German version of the HCUTI.</p><p><strong>Methods: </strong>Translation and linguistic validation was performed using the dual-panel method. A bilingual panel of nonprofessional Danish-German speaking persons translated the HCUTI, and the translated version was evaluated regarding comprehensibility by a lay panel of native German-speaking women with past uUTI. Content validity of the German version of the HCUTI was assessed according to the criteria of the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) methodology. In individual cognitive interviews, women with a history of uUTI and experts from different medical fields rated the instructions, items, response options and recall period of the HCUTI in terms of relevance, comprehensiveness and comprehensibility.</p><p><strong>Results: </strong>Translation and linguistic validation resulted in a German version of the HCUTI which lay persons considered easy to understand. In content validity assessments, participants rated the questionnaire as generally relevant and comprehensive. One item was removed due to lack of relevance. The response options were linguistically modified, and a dichotomous scale was introduced for 10 items on symptoms. To enhance comprehensibility, eight items on symptoms and bothersomeness, and one item on daily activities were slightly reformulated. After modification, the German version of the HCUTI includes 18 items on symptoms, 18 items on bothersomeness, and six items on impact on daily activities. The study team reviewed and linguistically standardized the final version of the questionnaire to ensure consistency in wording and sentence structure across all items.</p><p><strong>Conclusions: </strong>The German version of the HCUTI is a suitable tool to assess symptoms, bothersomeness and impact of uUTIs in women. Data on psychometric properties of the instrument will be collected in a subsequent survey among women with uUTIs.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Mechanisms of Microenergy Acoustic Pulse Therapy for the Treatment of Female Stress Urinary Incontinence-Part II.","authors":"Guiting Lin, Liangyu Zhao, Chen Yang, Emily Xing, Tian Bai, Thomas Cao, Guifang Wang, Lia Banie, Yingchun Zhang, Yuxin Tang, Tom F Lue","doi":"10.1002/nau.70048","DOIUrl":"https://doi.org/10.1002/nau.70048","url":null,"abstract":"<p><strong>Aims: </strong>While previous studies showed that micro-energy acoustic pulse (MAP) therapy restores urethral structure and function in adult stress urinary incontinence (SUI) rats, the underlying mechanisms remain unclear. This study aimed to explore the effect of MAP therapy in a rat model of vaginal birth injury-induced SUI at single cell resolution.</p><p><strong>Methods: </strong>Rat model of SUI was established using vaginal balloon dilation plus ovariectomy combined with β-aminopropionitrile treatment. Subsequently, MAP therapy was administered twice weekly for a total of 4 weeks. At the end of the treatment, single-cell sequencing was performed to analyze changes of cell heterogeneity and signaling in the urethral tissue microenvironment. Functional evaluations, including leak point pressure (LPP) measurements, electromyography, and electrophysiological studies, were conducted to confirm the effects of MAP therapy on urethral repair and muscle reinnervation.</p><p><strong>Results: </strong>MAP enhanced the transcription levels of various cell proliferation markers, including Pcna, Mki67, and Ccne2. The MAP treatment group exhibited higher muscle fiber content and structural integrity in the urethral sphincter compared to the untreated SUI model group. MAP also improved LPP, reduced sudden jumps in the CMAP value, and activated pathways such as \"ribosome\" in muscle cells. Additionally, MAP reduced senescence-associated beta-galactosidase levels in the urethral epithelium and influenced the expression of multiple transcription factors.</p><p><strong>Conclusions: </strong>MAP therapy effectively promotes urethral repair by modulating cellular aging and improving muscle reinnervation. These findings provide valuable insights into urethral repair mechanisms and lay the groundwork for developing novel therapies for SUI.</p><p><strong>Clinical trial registration: </strong>This study is based on an animal model and does not require a clinical trial registration.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor: Deep Learning and Numerical Analysis for Bladder Outflow Obstruction and Detrusor Underactivity Diagnosis in Men: A Novel Urodynamic Evaluation Scheme.","authors":"Wouter van Dort, Thomas R F van Steenbergen, Peter F W M Rosier","doi":"10.1002/nau.70049","DOIUrl":"https://doi.org/10.1002/nau.70049","url":null,"abstract":"","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging the Gap in Patient Guidance: Quality Analysis of Kegel Exercise Videos on YouTube.","authors":"Nazım Furkan Gunay, Sedat Cakmak, Mucahit Gelmis, Caglar Dizdaroglu","doi":"10.1002/nau.70070","DOIUrl":"https://doi.org/10.1002/nau.70070","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the content and quality of YouTube videos on Kegel exercises, focusing on their reliability for patients managing conditions such as premature ejaculation, urinary incontinence, and post-pelvic surgery recovery.</p><p><strong>Methods: </strong>A cross-sectional analysis was conducted using the keyword \"Kegel exercises\" on YouTube. The top 100 videos were screened, and 60 met the inclusion criteria. Videos were evaluated using the Kegel Video Evaluation Score (KVES) and the Global Quality Scale (GQS). Video metrics such as duration, views, likes, and comments were recorded, and statistical analyses were performed to assess correlations and inter-rater reliability.</p><p><strong>Results: </strong>The mean KVES and GQS scores were 17.26 ± 4.38 and 3.36 ± 1.09, respectively, with 60% of videos categorized as high quality. Nonphysician health professionals uploaded the highest-quality videos for instructional content. A strong correlation between KVES and GQS was observed (Spearman's ρ = 0.924, p < 0.001), validating the evaluation tool. Video duration positively impacted content comprehensiveness, while views and likes did not correlate with quality.</p><p><strong>Conclusions: </strong>In contrast to previous studies highlighting low-quality medical content on YouTube, this study shows that high-quality Kegel exercise videos are prevalent, suggesting their potential as reliable educational resources. Clinician involvement is essential in guiding patients to trustworthy content and fostering collaboration with content creators to improve digital health information.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Application of T1ρ Magnetic Resonance Imaging for Noninvasive Assessment of Bladder Hyperpermeability Biomarkers: A Focus on Glycosaminoglycan Content.","authors":"Julie A Suyama, Sheronda M Statum, Aurea V R Mohana-Borges, Xin Cheng, Yajun Ma, Jeffrey D Esko, Jennifer T Anger, Christine B Chung, Marianna Alperin","doi":"10.1002/nau.70058","DOIUrl":"https://doi.org/10.1002/nau.70058","url":null,"abstract":"<p><strong>Purpose: </strong>Bladder wall hyperpermeability due to glycosaminoglycan depletion is implicated in interstitial cystitis/bladder pain syndrome pathogenesis. This study sought to validate T1ρ MRI as a noninvasive imaging sequence for assessing bladder wall hyperpermeability biomarkers, with a focus on bladder glycosaminoglycan content, in protamine sulfate-induced models of interstitial cystitis/bladder pain syndrome.</p><p><strong>Materials and methods: </strong>Rat bladders (n = 8) treated with saline (control), protamine sulfate, pentosan polysulfate, or protamine sulfate + pentosan polysulfate (rescue) were imaged in situ using T1ρ and standard MRI sequences. Predominant bladder glycosaminoglycans, chondroitin and heparan sulfate, were measured in subsequently harvested rat bladders via aniline tagging coupled with mass spectrometry. Human bladder biopsies (n = 12) were similarly imaged before and after protamine sulfate treatment, and post-imaging glycosaminoglycan analysis was performed. Data were compared between groups using one-way ANOVA or paired Student t-test. T1ρ relaxivity was correlated with chemically measured glycosaminoglycan content using linear regression.</p><p><strong>Results: </strong>Protamine sulfate-treated rat bladders had decreased glycosaminoglycans and higher T1ρ relaxivity relative to controls. Pentosan polysulfate also decreased glycosaminoglycans versus controls and did not mitigate protamine-mediated glycosaminoglycan depletion. Importantly, T1ρ relaxivity correlated with chemical glycosaminoglycan quantification (chondroitin sulfate: r = 0.86, p < 0.01; heparan sulfate r = 0.80, p = 0.02). In human biopsies, T1ρ relaxivity increased after protamine sulfate treatment versus baseline (154.2 ± 5.9 vs. 131.0 ± 4.4 ms, p < 0.001), consistent with decreased glycosaminoglycans, while chemical analyses failed to capture statistically significant changes in bladder glycosaminoglycans.</p><p><strong>Conclusions: </strong>T1ρ MRI accurately measured glycosaminoglycans in rat bladders and differentiated protamine sulfate-treated bladder biopsies from unperturbed specimens in humans. T1ρ MRI warrants further investigation as a novel biomarker of bladder glycosaminoglycan content in interstitial cystitis/bladder pain syndrome.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time Bladder Volume Monitoring for Pediatric Patients Using a Commercially Available Wearable Ultrasound Device.","authors":"Peter Y Cai, Tanya Logvinenko, Badar Omar, Siam Oottamasathien, Michael Kurtz, Carlos Estrada, Stuart Bauer, Caleb P Nelson","doi":"10.1002/nau.70060","DOIUrl":"https://doi.org/10.1002/nau.70060","url":null,"abstract":"<p><strong>Introduction: </strong>We tested whether a commercially available, wearable bladder ultrasound device (DFree^Ⓡ, Triple W Japan Inc.) could accurately estimate bladder volume in children with urologic conditions by comparing sensor readings to instilled volume during cystometrography (CMG).</p><p><strong>Materials and methods: </strong>We prospectively enrolled 40 children (6 months to 18 years old). The device was taped to the suprapubic region and volume measurements obtained during CMG. Primary outcome was device accuracy, defined as device indicating ≥ 8 (0-10 scale) when the instilled volume was ≥ 80% of CMG-determined capacity. Mixed effects linear regression modeled the relationship between sensor indication and instilled volume after adjusting for clinical variables.</p><p><strong>Results: </strong>Median age was 7.2 years, 20 (50%) were male, 27 (67.5%) were on clean intermittent catheterization, 30 (75%) had spina bifida. The sensor accurately measured capacity in 46.2% of patients with a sensitivity of 0.70, specificity of 0.13, positive predictive value of 0.53, and negative predictive value of 0.22. Device readings were statistically associated with instilled volume; single-digit (10%) increase in sensor reading was associated with an average increase of 10% (95% CI: 9% to 12%, p < 0.001) in bladder volume.</p><p><strong>Conclusion: </strong>A commercially available, wearable ultrasound bladder sensor accurately measured bladder capacity in 46.2% of children undergoing UDS. Our findings caution clinicians in recommending these types of direct-to-consumer devices. Repeated measures testing is needed to determine if the accurate device indicators observed in a minority of patients herein, are reproducible.</p><p><strong>Trial registration: </strong>Our study did not need to be registered per the International Committee of Medical Journal Editors Uniform Requirements.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between Urinary Incontinence, Mortality, and Frailty: Insights From a Large Cohort Study.","authors":"Niharika Malviya, Tracy Cheung, Joseph Kim, Kelli Aibel, Chihiro Okada, Nitya Abraham","doi":"10.1002/nau.70064","DOIUrl":"https://doi.org/10.1002/nau.70064","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to explore the relationship between urinary incontinence (UI) and mortality in women, with a focus on the potential role of frailty and variations across UI subtypes.</p><p><strong>Methods: </strong>We analyzed 8967 women aged 40 and above who were part of the National Health and Nutrition Examination Survey (NHANES). Kaplan-Meier survival curves and Cox regression analyses were performed to assess the relationship between urge UI (UUI), stress UI (SUI), other UI (OUI) and all-cause mortality. These models were adjusted for demographic variables and frailty scores.</p><p><strong>Results: </strong>Approximately 45.8% of participants reported UI in the past year. Initial analysis showed a significant association between UI and increased mortality risk (hazard ratio [HR] = 1.478, 95% confidence interval [CI] 1.317-1.657, p < 0.001), persisting after controlling for frailty (HR = 1.151, 95% CI 1.022-1.297, p = 0.021). However, after adjusting for frailty and demographic factors, this association became non-significant.</p><p><strong>Conclusion: </strong>Women with UI exhibited a significantly increased risk of all-cause mortality, but this association did not hold after accounting for both key demographics and frailty.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Fusion Protein 2-Modified Bone Marrow Mesenchymal Stem Cells Improved Hyperglycemia-Induced Schwann Cell Injury via Regulating Mitochondria-Associated Endoplasmic Reticulum Membranes.","authors":"Housheng Fu, Zhewen Ou, Fei Wang, Weifu Wang, Zhongyao Wang","doi":"10.1002/nau.70067","DOIUrl":"https://doi.org/10.1002/nau.70067","url":null,"abstract":"<p><strong>Objective: </strong>High glucose damages rat Schwann cells (SCs), which is closely related to the dysfunction of mitochondria-associated endoplasmic reticulum membranes (MAMs). Therefore, the present study aimed to investigate the protective effects and mechanisms of modified bone marrow mesenchymal stem cells (BMSCs) and mitochondrial fusion protein 2 (Mfn2) modified BMSCs against SCs injury.</p><p><strong>Methods: </strong>The Mfn2-modified BMSCs were constructed after culturing with neural-induced differentiation solution. MAP-2 (microtubule-associated protein-2, neuron marker) and GFAP (glial fibrillary acidic protein, astrocytes marker) immunofluorescence staining was used to observe changes in the differentiation potential of neural-like BMSCs. SCs (RSC96) cells cultured under high glucose conditions were cocultured with Mfn2-modified BMSCs. Changes in functional protein expression of MAMs were detected by Western Blot. Transmission electron microscopy (TEM) was used to observe the microscopic morphology of MAMs, mitochondria and endoplasmic reticulum.</p><p><strong>Results: </strong>The expression level of Mfn2 was significantly increased in BMSCs transfected with Mfn2. The fluorescence densities of MAP-2 and GFAP were significantly upregulated in Mfn2-BMSCs after induction by neural inducible differentiation solution. When RSC96 was incubated with high glucose and Mfn2-modified/non-modified BMSCs, the expression level of Mfn2 in RSC96 was significantly increased, while PERK, IP3R and Drp1 expressions were significantly reduced. And the Mfn2-modified BMSCs showed more significant effects comparing to Mfn2-non-modified BMSCs. The TEM showed the structural integrity of MAMs, clear structure of mitochondrial cristae and obvious and structurally intact extension of endoplasmic reticulum in Mfn2-BMSC group.</p><p><strong>Conclusions: </strong>Mfn2 transfection promoted neural-like cell differentiation in BMSCs. Mfn2-modified BMSCs modulated the structural and functional homeostasis of MAMs by regulating the expression levels of MAMs functional proteins.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}