New biotechnology最新文献_第5页

WITHDRAWN: Catechol compounds and SpyCatcher-mediated immobilization of xylanase and lichenase chimera from cell lysates: A sustainable approach for biotechnological applications. 儿茶酚化合物和spycatch介导的细胞裂解物木聚糖酶和地衣酶嵌合体的固定化：生物技术应用的可持续途径。

IF 5.6 2区生物学

New biotechnology Pub Date : 2026-02-14 DOI: 10.1016/j.nbt.2026.02.003

Yanhong Zhou, Zijiao Yang, Yaxin Chen, Ruifang Zhang, Chun Yang, Zhiqiang Lin, Wei Jiang, Guangya Zhang

{"title":"WITHDRAWN: Catechol compounds and SpyCatcher-mediated immobilization of xylanase and lichenase chimera from cell lysates: A sustainable approach for biotechnological applications.","authors":"Yanhong Zhou, Zijiao Yang, Yaxin Chen, Ruifang Zhang, Chun Yang, Zhiqiang Lin, Wei Jiang, Guangya Zhang","doi":"10.1016/j.nbt.2026.02.003","DOIUrl":"10.1016/j.nbt.2026.02.003","url":null,"abstract":"<p><p>A mild and green immobilization strategy was proposed based on catechol compounds and elastin-like peptides-SpyCatcher (EC), where ECs could specifically bind to the target enzyme chimera (xylanase-SpyTag-lichenase, XL) and offer suitable arm length between the chimera and carriers. The EC-functionalized carriers (Poly(vinylidene fluoride), PVDF) exhibited excellent carrier stability. After incubation at 4 ℃ for 12 days, the retention rate of EC was as high as 92.3%. When applied for covalently immobilizing XL from the cell crude lysates, it achieved the enzyme load of 34.1μg/cm<sup>2</sup>. The immobilized XL showed enhanced affinity towards the substrates and excellent thermostability, where the half-lives of xylanase and lichenase in XL at 50 ℃ were significantly improved up to 632.8% and 1266.1%, respectively. The versatility of this strategy was also proved by the carriers of nylon and gauze, which also showed comparable kinetic parameters with those immobilized on PVDF. The encouraging results pave a sustainable and eco-friendly way for purification and immobilization of target enzymes in a single step, which offers a potentially renewable avenue for biocatalysis and other related biotechnological processes.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocrystallization: Harnessing natural scaffolds for stable protein crystal formulations 生物结晶：利用天然支架稳定的蛋白质晶体配方

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-10-25 DOI: 10.1016/j.nbt.2025.10.010

Nevin Jaison , Kavita Yadav , Huaiyu Yang , Vikash Yadav

{"title":"Biocrystallization: Harnessing natural scaffolds for stable protein crystal formulations","authors":"Nevin Jaison , Kavita Yadav , Huaiyu Yang , Vikash Yadav","doi":"10.1016/j.nbt.2025.10.010","DOIUrl":"10.1016/j.nbt.2025.10.010","url":null,"abstract":"<div><div>There is a growing interest in developing precisely engineered and reliable crystalline formulations of proteins for medical and biotechnological applications. However, protein crystallization remains a complex and often unpredictable process, influenced by numerous factors governing nucleation and crystal growth. Furthermore, industrial-scale crystallization and purification techniques are still underdeveloped, presenting a significant bottleneck for broad implementation. Consequently, innovative strategies are required to address the challenges associated with large-scale crystal particle formulation, particularly for fragile therapeutic and industrial relevant proteins. Certain natural protein scaffolds possess intrinsic sequence features that promote spontaneous crystal lattice formation, often serving functional roles in their native environment. These scaffolds can drive crystal formation either in native stage or with foreign cargo proteins through heterologous expression in recombinant host cells. By leveraging these properties, target proteins can be packed into stable crystalline matrices that support encapsulation, protection, and sustained release of functional cargo proteins. Such scaffold-based in cellulo systems offer advantages in terms of reproducibility, manipulability, and potential for nanoscale engineering. They are emerging as powerful platforms for drug delivery, protein immobilisation, and functional biomaterials. This review provides a comprehensive overview of scaffold-assisted in-cell biocrystallization of cargo proteins. We discussed naturally occurring crystalline scaffolds, their fusion design strategies, potential applications, and highlighted critical challenges and future directions. Together, these insights illustrate how harnessing natural crystallization mechanisms can enable the next generation of protein-based nanoformulations for healthcare, catalysis, and synthetic biology.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 257-267"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation at a liquid–liquid interface prevents phenotypic heterogeneity of human induced pluripotent stem-derived retinal pigment epithelial cells 在液-液界面培养可防止人诱导的多能性干源性视网膜色素上皮细胞的表型异质性。

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-10-14 DOI: 10.1016/j.nbt.2025.10.006

Rie Sonoi , Masamichi Kamihira

{"title":"Cultivation at a liquid–liquid interface prevents phenotypic heterogeneity of human induced pluripotent stem-derived retinal pigment epithelial cells","authors":"Rie Sonoi , Masamichi Kamihira","doi":"10.1016/j.nbt.2025.10.006","DOIUrl":"10.1016/j.nbt.2025.10.006","url":null,"abstract":"<div><div>Culturing retinal pigment epithelial (RPE) cells, a valuable source for drug discovery and cell transplantation therapies, on a polystyrene solid interface often induces spontaneous phenotypic heterogeneity, including cobblestone-shaped, dome-shaped, and stratified cells within a passaged cell population. Understanding and regulating these phenotypic changes is essential for producing high-quality and safe cell sources. In this study, we developed a cultivation strategy to promote the uniform maturation of human induced pluripotent stem (hiPS)-derived RPE cells by focusing on their behavior in a culture vessel. hiPS-RPE cells cultured at the solid–liquid interface exhibited phenotypic heterogeneity, characterized by cobblestone, dome-shaped, and stratified morphologies, indicating RPE phenotype shifts associated with cellular senescence. However, replacing the Rho-associated coiled-coil kinase (ROCK) inhibitor Y27632 with forskolin, which enhances cell-cell and cell-substrate adhesion, facilitated uniform maturation of confluent hiPS-RPE cells on a laminin-332-coated liquid–liquid interface. Quantitative analysis revealed that the levels of tight junction formation, <em>F</em><sub>Z</sub>, and the homogeneity index, i.e., the degree of uniform cell distribution, <em>H</em><sub>LN</sub>, were consistent between the central and peripheral regions of the culture vessel (<em>F</em><sub>Z</sub> = 0.97, <em>H</em><sub>LN</sub> = 0.95). These findings highlight the importance of using a liquid–liquid interface to suppress spontaneous phenotypic heterogeneity by promoting uniform cell distribution. Our study presents a novel methodology for efficiently achieving uniform maturation of functional hiPS-RPE cells at the liquid–liquid interface within a culture vessel.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 196-205"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reengineering of ergosterol biosynthesis in Saccharomyces cerevisiae and its sustainable application in citrus peel processing industry 酿酒酵母麦角甾醇生物合成的再造及其在柑橘果皮加工业中的可持续应用

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-10-08 DOI: 10.1016/j.nbt.2025.10.002

Hui Chen , Yasi Wang , Yan Zhang , Nan Peng , Suping Zhang , Yixuan She , Yuxian Yang , Xiuji Liu , Zhangshuang Deng , Xiaosong Gu

{"title":"Reengineering of ergosterol biosynthesis in Saccharomyces cerevisiae and its sustainable application in citrus peel processing industry","authors":"Hui Chen , Yasi Wang , Yan Zhang , Nan Peng , Suping Zhang , Yixuan She , Yuxian Yang , Xiuji Liu , Zhangshuang Deng , Xiaosong Gu","doi":"10.1016/j.nbt.2025.10.002","DOIUrl":"10.1016/j.nbt.2025.10.002","url":null,"abstract":"<div><div>Ergosterol is an important sterol primarily found in fungi, yeast, and certain plants, with particularly high concentrations in yeast and fungus. As the precursor to vitamin D<sub>2</sub>, it is converted into vitamin D<sub>2</sub> upon exposure to ultraviolet (UV) radiation. In this study, we successfully reengineered the ergosterol biosynthesis in <em>Saccharomyces cerevisiae</em> through a systematic synthetic biology strategy, and explored its sustainable application in citrus peel processing industry by using the citrus peel hydrolysate as a fermentation substrate. Through the MVA pathway module, the post-squalene pathway module, and the ergosterol biosynthesis module strategies, the titer of ergosterol was significantly increased to 23.38 mg/g DCW, which was nearly 19.15-fold higher than that of the original strain. The engineered strain was capable of producing 2.35 and 5.65 g/L of ethanol at 24 h in shake flasks and 5-L bioreactor fermentation by using the citrus peel hydrolysate as a fermentation substrate, respectively. The conversion rate of ethanol from citrus peel powder was approximately 3.3 %. To the best of our knowledge, this is the first report on the reengineering of ergosterol biosynthesis in <em>Saccharomyces cerevisiae</em> for resisting the stress of D-limonene, implying the engineered strain offers a promising avenue for the development of sustainable and eco-friendly bioenergy solutions in citrus peel processing industry.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 155-162"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A human genome wide screen identifies miRNAs to increase recombinant Adeno-associated virus production in HEK293 cells 人类基因组宽筛选鉴定mirna增加重组腺相关病毒在HEK293细胞的生产。

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-11-01 DOI: 10.1016/j.nbt.2025.10.009

Madina Burkhart , Bettina Finkbeiner , Jona Röscheise , Sabrina Reichl , Florian Sonntag , Markus Hörer , Andreas Schulze , Kerstin Otte

{"title":"A human genome wide screen identifies miRNAs to increase recombinant Adeno-associated virus production in HEK293 cells","authors":"Madina Burkhart , Bettina Finkbeiner , Jona Röscheise , Sabrina Reichl , Florian Sonntag , Markus Hörer , Andreas Schulze , Kerstin Otte","doi":"10.1016/j.nbt.2025.10.009","DOIUrl":"10.1016/j.nbt.2025.10.009","url":null,"abstract":"<div><div>Adeno-associated virus vectors have become increasingly important vehicles for gene therapy applications. However, the inability to produce vector with high yields and quality is a bottleneck hindering applicability to larger indications. microRNAs have successfully been utilized as cell engineering tools to optimize therapeutic protein production cell lines. The present study aimed at identifying miRNAs capable of increasing rAAV production in HEK293 cells by screening a miRNA mimic library. Out of 2428 miRNAs examined, 144 miRNAs increased functional rAAV titer. A secondary screen confirmed almost 50 % of miRNAs displaying the strongest positive effect. Further validation of six miRNA lead candidates hsa-miR-125a-5p, hsa-miR-135b-5p, hsa-miR-519c-5p, hsa-miR-563, hsa-miR-6857–3p and hsa-miR-3074–3p using plasmid-based miRNA expression and scale-up rAAV production in the Ambr®15 bioreactor system, confirmed their positive impact on functional rAAV titer without substantially compromising critical quality attributes. Bioinformatic analysis showed the miRNA-directed regulation of distinct viral sensing and signalling pathways, as well as apoptosis, which are hypothesized to be the underlying cause of the increased productivity. Our results emphasize the applicability of miRNAs as a powerful tool for cell line engineering capable of optimizing rAAV production in HEK293 cells.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 268-280"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How food wastes can be converted into new products: European legislation and analysis of enzymatic hydrolysis 如何将食物垃圾转化为新产品：欧洲立法和酶水解分析。

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-09-20 DOI: 10.1016/j.nbt.2025.09.005

Laura Esposito, Francesca Accardo, Barbara Prandi, Tullia Tedeschi

{"title":"How food wastes can be converted into new products: European legislation and analysis of enzymatic hydrolysis","authors":"Laura Esposito, Francesca Accardo, Barbara Prandi, Tullia Tedeschi","doi":"10.1016/j.nbt.2025.09.005","DOIUrl":"10.1016/j.nbt.2025.09.005","url":null,"abstract":"<div><div>The generated food waste has a significant economic and environmental impact. Since most of this is considered nutrient-rich substrate, it can be reduced or converted to avoid negative effects. In recent years, new technologies have increasingly focused on this aim by extracting and recovering valuable components for the formulation of new products. Herein, this review analyses food waste management strategies and emerging scientific advancements. Among these, enzymatic hydrolysis represents a promising sustainable alternative to traditional chemical extraction. Unlike chemical methods, it operates under mild conditions, reducing energy consumption and harmful by-products, while efficiently recovering fibres, proteins, phenolic compounds, and other biomolecules of interest from food waste. Furthermore, its effectiveness can be significantly enhanced when combined with other techniques. However, most of these applications are currently at the laboratory scale, and a thorough assessment of the potential benefits and the feasibility at an industrial level is required. In this context, integrating enzymatic hydrolysis within circular economy models can further improve resource efficiency by promoting waste valorisation in industrial applications. This approach aligns with sustainable development goals, fostering the creation of bio-based products and reducing dependence on non-renewable resources. Despite facing challenges such as regulatory constraints and the need for scalable, cost-effective processes, the development of innovative and sustainable practices can bring significant economic, social, and environmental benefits.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 122-133"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbially induced carbonate precipitation as an innovative technology for achieves efficient Sr2+ bioremediation 微生物诱导碳酸盐沉淀是实现高效Sr2+生物修复的创新技术。

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-10-30 DOI: 10.1016/j.nbt.2025.10.012

Zhimeng Su , Ting Chen , Fuchun Li , Xuelin Li , Fei Zhang , Yin Yi

{"title":"Microbially induced carbonate precipitation as an innovative technology for achieves efficient Sr2+ bioremediation","authors":"Zhimeng Su , Ting Chen , Fuchun Li , Xuelin Li , Fei Zhang , Yin Yi","doi":"10.1016/j.nbt.2025.10.012","DOIUrl":"10.1016/j.nbt.2025.10.012","url":null,"abstract":"<div><div>Microbes have demonstrated remarkable potential for Sr<sup>2+</sup> bioremediation under both laboratory and in situ conditions. However, the mechanism for efficient biological removal of toxic elements remains elusive. This study systematically investigated the efficacy and underlying mechanisms of Sr<sup>2+</sup> immobilization through microbially induced carbonate precipitation (MICP) using <em>Sporosarcina pasteurii</em> in a simulated contaminated system. Experimental results revealed that the strain achieved simultaneous and efficient immobilization of both Sr<sup>2+</sup> and Ca<sup>2+</sup> within 72 h, with removal efficiencies exceeding 99.95 %. Solution chemistry analysis identified high concentrations of carbonate and bicarbonate ions, generated through bacterial ureolysis, as the key drivers of the efficient precipitation process. XRD, EDS, and XPS analyses confirmed the incorporation of Sr<sup>2+</sup> into crystal lattices of calcite and strontianite crystal lattices through isomorphic substitution. Intriguingly, the Sr/Ca molar ratio (0.4–0.6) in early-stage precipitates was substantially different from that in the initial solution. Complementary analyses using FTIR and 3D-EEM fluorescence spectroscopy further demonstrated that bacterial-secreted organic matter selectively regulated the preferential precipitation of Ca<sup>2+</sup>. As a result, the strain's exceptional mineralization capacity effectively compensated for this preferential effect, ensuring efficient Sr<sup>2+</sup> immobilization. This study demonstrates the application advantages of microbes in MICP technology, providing critical insights for the remediation of radioactive contamination as well as a promising strategy for carbon sequestration of microbe-derived organic matter.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 281-289"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Public perception of new plant breeding techniques for sustainable production of feed and food in the Czech Republic 公众对捷克共和国饲料和食品可持续生产的新植物育种技术的看法

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-08-13 DOI: 10.1016/j.nbt.2025.08.002

Naděžda Čadová , Jaroslav Doležel , Ivo Frébort

{"title":"Public perception of new plant breeding techniques for sustainable production of feed and food in the Czech Republic","authors":"Naděžda Čadová , Jaroslav Doležel , Ivo Frébort","doi":"10.1016/j.nbt.2025.08.002","DOIUrl":"10.1016/j.nbt.2025.08.002","url":null,"abstract":"<div><div>A survey was conducted among 1676 Czech citizens in the autumn of 2024 to explore their attitudes toward different methods of crop improvement, which are based on the modification of genetic information. The study focused on three techniques: classical mutagenesis, insertion of foreign genetic material, and genome editing. The findings revealed that a significant part of the general public was unfamiliar with these technologies and their legal status. A majority of respondents mistakenly believed that currently permitted mutation breeding using radiation or chemical mutagens is illegal, while conversely assuming that techniques strictly regulated in Europe, such as transgenesis and genome editing, are allowed. Despite using different survey methods and question wording, the study pointed to a modest positive shift in public attitudes toward new genetic technologies compared to earlier surveys. For example, support for legalising genome editing rose from 22 % in 2019 to 47.6 % in 2024, and the proportion of respondents who would buy food containing genetically modified ingredients increased from 35 % to 47.4 %. Acceptance of these methods varied across demographic groups, with younger, more educated, and male respondents showing higher levels of support. While many acknowledge potential benefits such as reduced pesticide use, concerns about safety, ethics, and environmental impact remain. In general, the Czech public demonstrates cautious optimism, underscoring the importance of transparency and clear product labelling.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 13-19"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144829657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viability assessment and biomass valorization of recombinant Komagataella phaffii 重组法菲Komagataella phaffii的活力评价与生物量增值。

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-08-05 DOI: 10.1016/j.nbt.2025.07.005

Sarah Gangl , Maik Hilgarth , Christian Spielhaupter , Frank Schneider , Christiane Herrmann , Christina Recher , Tobias Baumgartner , Martina Geier , Birgit Wassermann , Anton Glieder

{"title":"Viability assessment and biomass valorization of recombinant Komagataella phaffii","authors":"Sarah Gangl , Maik Hilgarth , Christian Spielhaupter , Frank Schneider , Christiane Herrmann , Christina Recher , Tobias Baumgartner , Martina Geier , Birgit Wassermann , Anton Glieder","doi":"10.1016/j.nbt.2025.07.005","DOIUrl":"10.1016/j.nbt.2025.07.005","url":null,"abstract":"<div><div><em>Komagataella phaffii</em> has become a key player in biotechnology used for the production of heterologous proteins with numerous GRAS notices and QPS status released in the past years. While most of these descriptions and scientific studies focus on strain engineering, products and cultivation processes, this paper provides fundamental data about the general viability of <em>K. phaffii</em> after post-production biomass treatments and co-incubations with non-sterile soil and water samples. A comparison of different <em>K. phaffii</em> strains uncovered a high thermal sensitivity for all tested strains, which was further enhanced under alkaline conditions. A rapid reduction in the viable cell number started at approx. 52 °C and terminated in a full loss of 10<sup>9</sup> viable cells per milliliter within seconds at 70 °C. Survival rates of untreated non-GMO and GMO <em>K. phaffii</em> strains in water- and soil-slurry-microcosms demonstrated that the number of viable <em>K. phaffii</em> cells decreased rapidly and comparably to a <em>Saccharomyces cerevisiae</em> reference. No mating events were detected under these environmental conditions. These findings supported assumptions that <em>K. phaffii</em> is highly specialized and not a ubiquitous microorganism as described for other yeasts. Finally, intrigued by the low survival outside optimal laboratory conditions, <em>K. phaffii</em> biomass was evaluated in mesophilic anaerobic digestion processes for energy production. Enhanced methane yields and a concomitant loss of viable <em>K. phaffii</em> cells were demonstrated.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 1-12"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a hybrid integrated bottled photobioreactor system for enhanced biomass yield and CO2 fixation during Limnospira fusiformis cultivation 设计一种混合集成的瓶装光生物反应器系统，以提高毛状Limnospira fusformis培养过程中的生物量产量和二氧化碳固定。

IF 4.9 2区生物学

New biotechnology Pub Date : 2025-12-25 Epub Date: 2025-11-04 DOI: 10.1016/j.nbt.2025.10.014

Ameer Ali Kubar , Santosh Kumar , Shahid Mehmood , Michael Schagerl , Xinjuan Hu , Xiangru Xu , Feifei Zhu , Ebin Gao , Shuhao Huo

{"title":"Designing a hybrid integrated bottled photobioreactor system for enhanced biomass yield and CO2 fixation during Limnospira fusiformis cultivation","authors":"Ameer Ali Kubar , Santosh Kumar , Shahid Mehmood , Michael Schagerl , Xinjuan Hu , Xiangru Xu , Feifei Zhu , Ebin Gao , Shuhao Huo","doi":"10.1016/j.nbt.2025.10.014","DOIUrl":"10.1016/j.nbt.2025.10.014","url":null,"abstract":"<div><div>This study introduces a novel approach to optimizing the cultivation of <em>Limnospira fusiformis</em> through a hybrid Integrated Bottled Photobioreactor (ITB-PBR). By combining this ITB-PBR with an open raceway pond, the system achieved substantial improvements in biomass yield around 38 % and 55 % higher than hybrid traditional PBR and open raceway pond, respectively, and a 42 % higher areal productivity relative to the hybrid traditional PBR. The hybrid ITB-PBR enhanced CO<sub>2</sub> fixation and photosynthetic efficiency, due to its efficient mixing and mass transfer capabilities. The integration of bottles within the column PBR reduced mixing time by 13 % and increased the mass transfer coefficient by 16 %. Additionally, the optimized design of the hybrid ITB-PBR, through generation of vortices within its sections, facilitated efficient movement of microalgae between light and dark regions, leading to a 9 % boost in photochemical efficiency (Fv/Fm) and a 5 % increase in chlorophyll content. The hybrid ITB-PBR also showed a remarkable 79 % increase in CO<sub>2</sub> fixation compared to conventional reactor. The hybrid ITB-PBR ensured continuous access to both light and carbon sources, resulting in higher biomass yields and enhanced growth by overcoming the common challenges of hybrid traditional PBR, such as poor light penetration and insufficient CO<sub>2</sub> dissolution.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 290-297"},"PeriodicalIF":4.9,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145459361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0